The real in time computerized tomography (CT) testing of the meso damage propagation law of the whole sandstone failure process under triaxial compression has been completed using the newest specified triaxial loading...The real in time computerized tomography (CT) testing of the meso damage propagation law of the whole sandstone failure process under triaxial compression has been completed using the newest specified triaxial loading equipment corresponding to the CT machine. Through the CT scanning, the clear CT images which include from the microcracks compressed stage to growth stage, bifurcation stage, development stage, crack fracture stage,the rock sample failure until to unloading stage in the different stress states were obtained. The CT values, CT images and the other data have been analyzed. Based on the results of the CT testing of meso damage evolution law of rock,the stress threshold value of meso damage of rock is given, and the stress strain complete process curve of rock is divided into some sections. The initial rock damage propagation law is given in this paper.展开更多
Although various strategies have been proposed for enrichment of circulating tumor cells(CTCs),the clinical outcomes of CTC detection are far from satisfactory.The prevailingmethodologies for CTC detection are general...Although various strategies have been proposed for enrichment of circulating tumor cells(CTCs),the clinical outcomes of CTC detection are far from satisfactory.The prevailingmethodologies for CTC detection are generally oriented towardnaturallyoccurring targets;however,misdetection and interference are prevalent due to the diverse phenotypes and subpopulations of CTCs,which are highly heterogeneous.Here,a CTC isolation system based on the“labelcapture-release”process is demonstrated for the precise and highly efficient enrichment of CTCs fromclinical blood samples.On the basis of the abnormal glycometabolism of tumor cells,the surface of CTCs can be decorated with artificial azido groups.By utilizing bio-orthogonal plates designed with dibenzocyclooctane(DBCO)and disulfide groups,withthe aid of anti-fouling effects,CTCs labeled with azido groups can be captured through a copper-free click reaction and subsequently released via disulfide reduction.The technique has been shown to label tumor cells with the epithelial cell adhesion molecule(EpCAM)+and EpCAM~phenotypes in both adherent and suspended states.Moreover,it effectively isolates all epithelial,interstitial,and hybrid phenotypes of CTCs from clinical blood samples collected from dozens of patients across more than 10 cancer types.Compared to the clinically approved CTC detection system,our strategy demonstrates superior performance from the perspective of broad-spectrum and accurate recognition of heterogeneous CTCs.More importantly,most of the captured CTCs can be released with the retention of living activity,making this technique well suited for downstream applications such as drug susceptibility tests involving viable CTCs.展开更多
基金FundofStateKeyLaboratoryofFrozenSoilEngineeringofChina !(No 980 2 No 2 0 0 3 )
文摘The real in time computerized tomography (CT) testing of the meso damage propagation law of the whole sandstone failure process under triaxial compression has been completed using the newest specified triaxial loading equipment corresponding to the CT machine. Through the CT scanning, the clear CT images which include from the microcracks compressed stage to growth stage, bifurcation stage, development stage, crack fracture stage,the rock sample failure until to unloading stage in the different stress states were obtained. The CT values, CT images and the other data have been analyzed. Based on the results of the CT testing of meso damage evolution law of rock,the stress threshold value of meso damage of rock is given, and the stress strain complete process curve of rock is divided into some sections. The initial rock damage propagation law is given in this paper.
基金supported by the National Key R&D Program of China(2021YFB3800800)the National Natural Science Foundation of China(81903057,82073284,32000962,82402491,and 82272157)+2 种基金Shenzhen Science and Technology Research Funding(JCYJ20200109115601720)the Hong Kong PDFS-RGC Postdoctoral Fellowship Scheme(PDFS2122-1S08 and CityU 9061014)Hong Kong HMRF(Health and Medical Research Fund)(2120972 and CityU 9211320)。
文摘Although various strategies have been proposed for enrichment of circulating tumor cells(CTCs),the clinical outcomes of CTC detection are far from satisfactory.The prevailingmethodologies for CTC detection are generally oriented towardnaturallyoccurring targets;however,misdetection and interference are prevalent due to the diverse phenotypes and subpopulations of CTCs,which are highly heterogeneous.Here,a CTC isolation system based on the“labelcapture-release”process is demonstrated for the precise and highly efficient enrichment of CTCs fromclinical blood samples.On the basis of the abnormal glycometabolism of tumor cells,the surface of CTCs can be decorated with artificial azido groups.By utilizing bio-orthogonal plates designed with dibenzocyclooctane(DBCO)and disulfide groups,withthe aid of anti-fouling effects,CTCs labeled with azido groups can be captured through a copper-free click reaction and subsequently released via disulfide reduction.The technique has been shown to label tumor cells with the epithelial cell adhesion molecule(EpCAM)+and EpCAM~phenotypes in both adherent and suspended states.Moreover,it effectively isolates all epithelial,interstitial,and hybrid phenotypes of CTCs from clinical blood samples collected from dozens of patients across more than 10 cancer types.Compared to the clinically approved CTC detection system,our strategy demonstrates superior performance from the perspective of broad-spectrum and accurate recognition of heterogeneous CTCs.More importantly,most of the captured CTCs can be released with the retention of living activity,making this technique well suited for downstream applications such as drug susceptibility tests involving viable CTCs.
