Amino acid bioconjugation technology has emerged as a pivotal tool for linking small-molecule fragments with proteins,antibodies,and even cells.The study in Nature by Chang and Toste introduces a redox-based strategy ...Amino acid bioconjugation technology has emerged as a pivotal tool for linking small-molecule fragments with proteins,antibodies,and even cells.The study in Nature by Chang and Toste introduces a redox-based strategy for tryptophan bioconjugation,employing N-sulfonyloxaziridines as oxidative cyclization reagents,demonstrating high efficiency comparable to traditional click reactions.Meanwhile,this tool provides feasible methods for investigating the mechanisms underlying functional tryptophan-related biochemical processes,paving the way for protein function exploration,activity-based proteomics for functional amino acid identification and characterization,and even the design of covalent inhibitors.展开更多
基金financial support from the National Natural Science Foundation of China(82374020 and 82374155)Science and Technology Department of Sichuan Province(2023ZYD0054 and 2024NSFTD0023)the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202209).
文摘Amino acid bioconjugation technology has emerged as a pivotal tool for linking small-molecule fragments with proteins,antibodies,and even cells.The study in Nature by Chang and Toste introduces a redox-based strategy for tryptophan bioconjugation,employing N-sulfonyloxaziridines as oxidative cyclization reagents,demonstrating high efficiency comparable to traditional click reactions.Meanwhile,this tool provides feasible methods for investigating the mechanisms underlying functional tryptophan-related biochemical processes,paving the way for protein function exploration,activity-based proteomics for functional amino acid identification and characterization,and even the design of covalent inhibitors.
