This paper focuses on the performance analysis of flexible reactive systems. The performance analysis consists of two phases： first system modeling, second performance evalua-tion. The paper models the flexible react...This paper focuses on the performance analysis of flexible reactive systems. The performance analysis consists of two phases： first system modeling, second performance evalua-tion. The paper models the flexible reactive system by the stochas-tic statecharts method, and uses the simulation method to evalu-ate the performance. To make use of the feature of event-triggered state transitions in the statecharts, a new method of simulation is proposed based on the techniques of the discrete-event system simulation. The new method solves the problem of computer imple-mentation of stochastic events, probabilistic transition, concurrent states, paral el actions, and broadcast communication mechanism in the stochastic statecharts. An example of a flexible manufactur-ing system is presented. The simulation result of the example is consistent with the analytical result, which shows the feasibility of the proposed new simulation method.展开更多
Reactive oxygen species(ROS)are essential in various pathological and physiological processes.Developing nanosystems that generate ROS in a controlled manner is of great interest for nanomedicine.DNA nanotechnology of...Reactive oxygen species(ROS)are essential in various pathological and physiological processes.Developing nanosystems that generate ROS in a controlled manner is of great interest for nanomedicine.DNA nanotechnology offers a promising approach to constructing programmable ROS-generating platforms.By incorporating photosensitizers or metal ions,DNA nanostructures can be designed to produce ROS in a spatially and temporally desired fashion.DNA-based ROS-generating nanosystems hold great potential in intracellular homeostasis regulation,drug release,and cancer therapy.This review summarizes recent advances in developing DNA-based ROS-generating nanosystems,highlights their emerging biomedical applications,and discusses the opportunities and challenges for further applications.DNA nanotechnology provides a versatile toolkit to construct biocompatible ROS-generating platforms for next-generation nanomedicines.展开更多
Astrocytes are the most abundant type of glial cell in the central nervous system.Upon injury and inflammation,astrocytes become reactive and undergo morphological and functional changes.Depending on their phenotypic ...Astrocytes are the most abundant type of glial cell in the central nervous system.Upon injury and inflammation,astrocytes become reactive and undergo morphological and functional changes.Depending on their phenotypic classification as A1 or A2,reactive astrocytes contribute to both neurotoxic and neuroprotective responses,respectively.However,this binary classification does not fully capture the diversity of astrocyte responses observed across different diseases and injuries.Transcriptomic analysis has revealed that reactive astrocytes have a complex landscape of gene expression profiles,which emphasizes the heterogeneous nature of their reactivity.Astrocytes actively participate in regulating central nervous system inflammation by interacting with microglia and other cell types,releasing cytokines,and influencing the immune response.The phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway is a central player in astrocyte reactivity and impacts various aspects of astrocyte behavior,as evidenced by in silico,in vitro,and in vivo results.In astrocytes,inflammatory cues trigger a cascade of molecular events,where nuclear factor-κB serves as a central mediator of the pro-inflammatory responses.Here,we review the heterogeneity of reactive astrocytes and the molecular mechanisms underlying their activation.We highlight the involvement of various signaling pathways that regulate astrocyte reactivity,including the PI3K/AKT/mammalian target of rapamycin(mTOR),αvβ3 integrin/PI3K/AKT/connexin 43,and Notch/PI3K/AKT pathways.While targeting the inactivation of the PI3K/AKT cellular signaling pathway to control reactive astrocytes and prevent central nervous system damage,evidence suggests that activating this pathway could also yield beneficial outcomes.This dual function of the PI3K/AKT pathway underscores its complexity in astrocyte reactivity and brain function modulation.The review emphasizes the importance of employing astrocyte-exclusive models to understand their functions accurately and these models are essential for clarifying astrocyte behavior.The findings should then be validated using in vivo models to ensure real-life relevance.The review also highlights the significance of PI3K/AKT pathway modulation in preventing central nervous system damage,although further studies are required to fully comprehend its role due to varying factors such as different cell types,astrocyte responses to inflammation,and disease contexts.Specific strategies are clearly necessary to address these variables effectively.展开更多
A new gas preparation system(GasPS-RCS)is proposed to solve two tasks:(A)to heat helium gas for tank pressurization;(B)to prepare gas for the Launch Vehicle(LV)Reactive Control System(RCS)at the LV orientation and sta...A new gas preparation system(GasPS-RCS)is proposed to solve two tasks:(A)to heat helium gas for tank pressurization;(B)to prepare gas for the Launch Vehicle(LV)Reactive Control System(RCS)at the LV orientation and stabilization sections of the LV on passive parts of the flight trajectory,to provide conditions for launching the Liquid Rocket Engine(LRE).The system includes a gas generator based on hydrogen peroxide,a separator for water separation,heat exchangers independent of the LRE,and gas-jet nozzles.The proposed new system allowed to reduce the length of pressurizing gas lines and reduce the increased helium gas consumption during the heat exchanger warm-up interval of the LRE during its launch.A special advantage of the proposed system is the possibility of ground testing of heat exchangers without an operating LRE.A mathematical model based on the first law of thermodynamics was used to perform a comparative analysis of GasPS-RCS with traditional pressurization and RCS systems.To validate the mathematical model,the experimental studies of helium pressurizing of a liquid nitrogen tank were conducted.The results show that the deviation of experimental and calculated values for pressure is 1.1%and for temperature up to 2%.According to the results of comparative analysis,the GasPS-RCS is 259 kg lighter for the first stage and 80 kg lighter for the second stage of the LV.展开更多
Considering the uncertainty of grid connection of electric vehicle charging stations and the uncertainty of new energy and residential electricity load,a spatio-temporal decoupling strategy of dynamic reactive power o...Considering the uncertainty of grid connection of electric vehicle charging stations and the uncertainty of new energy and residential electricity load,a spatio-temporal decoupling strategy of dynamic reactive power optimization based on clustering-local relaxation-correction is proposed.Firstly,the k-medoids clustering algorithm is used to divide the reduced power scene into periods.Then,the discrete variables and continuous variables are optimized in the same period of time.Finally,the number of input groups of parallel capacitor banks(CB)in multiple periods is fixed,and then the secondary static reactive power optimization correction is carried out by using the continuous reactive power output device based on the static reactive power compensation device(SVC),the new energy grid-connected inverter,and the electric vehicle charging station.According to the characteristics of the model,a hybrid optimization algorithm with a cross-feedback mechanism is used to solve different types of variables,and an improved artificial hummingbird algorithm based on tent chaotic mapping and adaptive mutation is proposed to improve the solution efficiency.The simulation results show that the proposed decoupling strategy can obtain satisfactory optimization resultswhile strictly guaranteeing the dynamic constraints of discrete variables,and the hybrid algorithm can effectively solve the mixed integer nonlinear optimization problem.展开更多
The penetration-deflagration coupling damage performance of rod-like reactive shaped charge pene-trator(RRSCP)impacting thick steel plates is investigated by theoretical analysis and experiments.A penetration-deflagra...The penetration-deflagration coupling damage performance of rod-like reactive shaped charge pene-trator(RRSCP)impacting thick steel plates is investigated by theoretical analysis and experiments.A penetration-deflagration coupling damage model is developed to predict the penetration depth and cratering diameter.Four type of aluminum-polytetrafluoroethylene-copper(Al-PTFE-Cu)reactive liners with densities of 2.3,2.7,3.5,and 4.5 g·cm^(-3) are selected to conduct the penetration experiments.The comparison results show that model predictions are in good agreement with the experimental data.By comparing the penetration depth and cratering diameter in the inert penetration mode and the penetration-deflagration coupling mode,the influence mechanism that the penetration-induced chemical response is unfavorable to penetration but has an enhanced cratering effect is revealed.From the formation characteristics,penetration effect and penetration-induced chemical reaction be-haviors,the influence of reactive liner density on the penetration-deflagration performance is further analyzed.The results show that increasing the density of reactive liner significantly increases both the kinetic energy and length of the reactive penetrator,meanwhile effectively reduces the weakened effect of penetration-induced chemical response,resulting in an enhanced penetration capability.However,due to the decreased diameter and potential energy content of reactive penetrator,the cratering capa-bility is weakened significantly.展开更多
Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder characterized by cognitive impairment and distinct neuropathological features,including amyloid-βplaques,neurofibrillary tangles,and reactive astrog...Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder characterized by cognitive impairment and distinct neuropathological features,including amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis.Developing effective diagnostic,preventative,and therapeutic strategies for AD necessitates the establishment of animal models that accurately recapitulate the pathophysiological processes of the disease.Existing transgenic mouse models have significantly contributed to understanding AD pathology but often fail to replicate the complexity of human AD.Additionally,these models are limited in their ability to elucidate the interplay among amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis due to the absence of spatially and temporally specific genetic manipulation.In this study,we introduce a novel AD mouse model(APP/PS1-TauP301L-Adeno mice)designed to rapidly induce pathological symptoms and enhance understanding of AD mechanisms.Neurofibrillary tangles and severe reactive astrogliosis were induced by injecting AAVDJ-EF1a-hTauP301L-EGFP and Adeno-GFAP-GFP viruses into the hippocampi of 5-month-old APP/PS1 mice.Three months post-injection,these mice exhibited pronounced astrogliosis,substantial amyloid-βplaque accumulation,extensiveneurofibrillarytangles,accelerated neuronal loss,elevated astrocytic GABA levels,and significant spatial memory deficits.Notably,these pathological features were less severe in AAVTauP301L-expressing APP/PS1 mice without augmented reactive astrogliosis.These findings indicate an exacerbating role of severe reactive astrogliosis in amyloid-βplaque and neurofibrillary tangle-associated pathology.The APP/PS1-TauP301L-Adeno mouse model provides a valuable tool for advancing therapeutic research aimed at mitigating the progression of AD.展开更多
A high-density tungsten-zirconium-titanium(W-Zr-Ti)reactive alloy was prepared by powder metallurgy.This alloy exhibits high density,high strength,and violent energy release characteristics,resulting in outstanding pe...A high-density tungsten-zirconium-titanium(W-Zr-Ti)reactive alloy was prepared by powder metallurgy.This alloy exhibits high density,high strength,and violent energy release characteristics,resulting in outstanding penetration and ignition abilities.Dynamic impact experiment demonstrated its strain rate hardening effect,and the energetic characteristics were investigated by digital image processing technique and thermal analysis experiment.The results show that W-Zr-Ti reactive alloy performs compressive strength of 2.25 GPa at 5784 s^(-1)strain rate,and its exothermic reaction occurs at about 961 K.Based on the explosion test and shock wave theory,thresholds of enhanced damage effect are less than 35.77 GPa and 5.18×10^(4)kJ/m^(2)for shock pressure and energy,respectively.Furthermore,the transformation of fracture behavior and failure mechanism is revealed,which causes the increase in compressive strength and reaction intensity under dynamic loading.展开更多
Reactive oxygen species(ROS),including singlet oxygen(^(1)O_(2)),hydroxyl radicals(·OH),and superoxide anions(O_(2)^(·-)),are highly reactive molecules that play central roles in many chemical,biological,and...Reactive oxygen species(ROS),including singlet oxygen(^(1)O_(2)),hydroxyl radicals(·OH),and superoxide anions(O_(2)^(·-)),are highly reactive molecules that play central roles in many chemical,biological,and environmental processes due to their strong oxidative power[1].Generating ROS in a controlled manner under mild conditions is essential for achieving selective oxidation reactions.Light-driven methods are especially appealing for this purpose,as they offer precise control over where and when ROS are produced.展开更多
Aiming to solve the problem of large discharge and severe pollution of reactive dyeing wastewater for wool fabrics,peroxodisulfate(SPS)was used for the degradation and recycling of dyeing wastewater containing reactiv...Aiming to solve the problem of large discharge and severe pollution of reactive dyeing wastewater for wool fabrics,peroxodisulfate(SPS)was used for the degradation and recycling of dyeing wastewater containing reactive dye Lanasol Red CE.The process of degrading the reactive dye was determined by using the dye residual rate as the evaluation index.The feasibility of reactive dyeing of wool fabrics using recycled dyeing wastewater was confirmed by measuring the dye uptake,exhaustion and fixation rates,as well as color parameters and fastness of the dyed fabrics.The results showed that the appropriate conditions for degrading Lanasol Red CE were 0.2 g/L SPS,an initial pH value of 3 and 100℃for 30 min.Under these conditions,the dye degradation rate was as high as 93.14%.When the recycled dyeing wastewater was used for dyeing of wool fabrics,the exhaustion rate of Lanasol Red CE exceeded 99%,and the fixation rate was higher than that achieved by the conventional dyeing process.Under the same dyeing conditions,the recycled-dyed fabrics appeared darker.When the number of cycles was fewer than five,the effect on color fastness was not obvious.Although the color fastness to rubbing and washing of the fabrics dyed in the 10th cycle decreased by half a grade and 1 grade,respectively,compared to that of the fabrics dyed with the conventional dyeing process,they still met the production requirements.展开更多
BACKGROUND Colon cancer is one of the most common malignancies worldwide,and chemo-therapy is a widely used strategy in colon cancer clinical therapy.Chemotherapy resistance is the main cause of recurrence and progres...BACKGROUND Colon cancer is one of the most common malignancies worldwide,and chemo-therapy is a widely used strategy in colon cancer clinical therapy.Chemotherapy resistance is the main cause of recurrence and progression in colon cancer.Thus,novel drugs for treatment are urgently needed.Tetramethylpyrazine(TMP),a component of the traditional Chinese medicine Chuanxiong Hort,has been proven to exhibit a beneficial effect in tumors.AIM To investigate the potential anticancer activity of TMP in colon cancer and the underlying mechanisms.METHODS Colon cancer cells were incubated with different concentrations of TMP.Cell viability was evaluated by crystal violet staining assay,and cell apoptosis was assessed by flow cytometry.Apoptosis-associated protein expression was measured using Western blot analysis.Intracellular reactive oxygen species(ROS)levels were assessed by flow cytometry using DCF fluorescence intensity.Xeno-grafts were established by the subcutaneous injection of colon cancer cells into nude mice;tumor growth was monitored and intracellular ROS was detected in tumors by malondialdehyde assay.RESULTS TMP induced apoptosis of colon cancer cells via the activation of the mitochon-drial pathway.TMP increased the generation of intracellular ROS and triggered mitochondria-mediated apoptosis in a caspase-dependent manner.CONCLUSION Our study demonstrates that TMP induces the apoptosis of colon cancer cells and increases the generation of intracellular ROS.TMP triggers mitochondria-mediated apoptosis in a caspase-dependent manner.The accumu-lation of intracellular ROS is involved in TMP-induced apoptosis.Our findings suggest that TMP may be a potential therapeutic drug for the treatment of colon cancer.展开更多
Ca^(2+)signaling plays crucial roles in plant stress responses,including defense against insects.To counteract insect feeding,different parts of a plant deploy systemic signaling to communicate and coordinate defense ...Ca^(2+)signaling plays crucial roles in plant stress responses,including defense against insects.To counteract insect feeding,different parts of a plant deploy systemic signaling to communicate and coordinate defense responses,but little is known about the underlying mechanisms.In this study,micrografting,in vivo imaging of Ca^(2+)and reactive oxygen species(ROS),quantification of jasmonic acid(JA)and defensive metabolites,and bioassay were used to study how Arabidopsis seedlings regulate systemic responses in leaves after hypocotyls are wounded.We show that wounding hypocotyls rapidly activated both Ca^(2+)and ROS signals in leaves.RBOHD,which functions to produce ROS,along with two glutamate receptors GLR3.3 and GLR3.6,but not individually RBOHD or GLR3.3 and GLR3.6,in hypocotyls regulate the dynamics of systemic Ca^(2+)signals in leaves.In line with the systemic Ca^(2+)signals,after wounding hypocotyl,RBOHD,GLR3.3,and GLR3.6 in hypocotyl also cooperatively regulate the transcriptome,hormone jasmonic acid,and defensive secondary metabolites in leaves of Arabidopsis seedlings,thus controlling the systemic resistance to insects.Unlike leaf-to-leaf systemic signaling,this study reveals the unique regulation of wounding-induced hypocotyl-to-leaf systemic signaling and sheds new light on how different plant organs use complex signaling pathways to modulate defense responses.展开更多
The electrokinetic(EK)process has been proposed for soil decontamination from heavy metals and organic matter.The advantages of the EK process include the low operating energy,suitability for fine-grained soil deconta...The electrokinetic(EK)process has been proposed for soil decontamination from heavy metals and organic matter.The advantages of the EK process include the low operating energy,suitability for fine-grained soil decontamination,and no need for excavation.During the last three decades,enhanced and hybrid EK systems were developed and tested for improving the efficiency of contaminants removal from soils.Chemically enhanced-EK processes exhibited excellent efficiency in removing contaminants by controlling the soil pH or the chemical reaction of contaminants.EK hybrid systems were tested to overcome environmental hurdles or technical drawbacks of decontamination technologies.Hybridization of the EK process with phytoremediation,bioremediation,or reactive filtermedia(RFM)improved the remediation process performance by capturing contaminants or facilitating biological agents’movement in the soil.Also,EK process coupling with solar energy was proposed to treat off-grid contaminated soils or reduce the EK energy requirements.This study reviews recent advancements in the enhancement and hybrid EK systems for soil remediation and the type of contaminants targeted by the process.The study also covered the impact of operating parameters,imperfect pollution separation,and differences in the physicochemical characteristics and microstructure of soil/sediment on the EK performance.Finally,a comparison between various remediation processes was presented to highlight the pros and cons of these technologies.展开更多
In the current study novel magnetic BiOBr-Gd^(3+)doped CoFe_(2)O_(4)heterojunction nanocomposites fabricated by chemical precipitation method for Acid Violet 7(AV 7)and Reactive Red 120(RR 120)dye degradation under su...In the current study novel magnetic BiOBr-Gd^(3+)doped CoFe_(2)O_(4)heterojunction nanocomposites fabricated by chemical precipitation method for Acid Violet 7(AV 7)and Reactive Red 120(RR 120)dye degradation under sunlight are presented.The samples were well characterized by X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM),scanning electron microscopy(SEM),energy dispersive X-ray analysis(EDAX),UV-visible near infrared(NIR),photoluminescence(PL),TRF,EIS,Mott-Schottky,and BET analysis.X-ray diffractograms of BiOBr-Gd^(3+)doped CoFe_(2)O_(4)nanocomposites authenticate the formation of the composite composed of diffraction peaks of both BiOBr and Gd^(3+)doped CoFe_(2)O_(4)samples.Photoluminescence(PL),Time Resolved Fluorescence Spectrocopy(TRF)and Electrochemical Impedance Spectroscopy(EIS)investigations were explored to examine the transfer efficiency of photoinduced charge carriers.Among the synthesized materials,the BiOBr-Gd^(3+)doped CoFe_(2)O_(4)-2 nanocomposite presents superior degradation capability for RR 120 of 87.34%and AV 7 dyes of 98.41%.The apparent rate constant(k)value of 0.1317 min^(-1)for AV 7 is sixteen times higher and RR 120(0.0135 min^(-1))is 2 times higher than pristine BiOBr.The radical trapping experiments also proved the role of photogenerated h^(+),O_(2)·^(-),and·OH radicals in the degradation mechanism.The superior performance of BiOBr-Gd^(3+)doped CoFe_(2)O_(4)-2 is ascribed to the formation of the heterostructure,the in-built electric field,and energy-band alignment,which thermodynamically and kinetically favors the photoactivity and stability of the heterojunction.展开更多
Herein, the electrochemical behaviors of Sr on inert W electrode and reactive Zn/Al electrodes were systematically investig-ated in LiCl–KCl–SrCl2molten salts at 773 K using various electrochemical methods. The chem...Herein, the electrochemical behaviors of Sr on inert W electrode and reactive Zn/Al electrodes were systematically investig-ated in LiCl–KCl–SrCl2molten salts at 773 K using various electrochemical methods. The chemical reaction potentials of Li and Sr on re-active Zn/Al electrodes were determined. We observed that Sr could be extracted by decreasing the activity of the deposited metal Sr onthe reactive electrode, although the standard reduction potential of Sr(II)/Sr was more negative than that of Li(I)/Li. The electrochemicalextraction products of Sr on reactive Zn and Al electrodes were Zn13Sr and Al4Sr, respectively, with no codeposition of Li observed.Based on the density functional theory calculations, both Zn13Sr and Al4Sr were identified as stable intermetallic compounds with Zn-/Al-rich phases. In LiCl–KCl molten salt containing 3wt% SrCl2, the coulombic efficiency of Sr in the Zn electrode was ~54%. The depolar-ization values for Sr on Zn and Al electrodes were 0.864 and 0.485 V, respectively, exhibiting a stronger chemical interaction between Znand Sr than between Al and Sr. This study suggests that using reactive electrodes can facilitate extraction of Sr accumulated while elec-trorefining molten salts, thereby enabling the purification and reuse of the salt and decreasing the volume of the nuclear waste.展开更多
BACKGROUND High levels of acetaminophen(APAP)consumption can result in significant liver toxicity.Mogroside V(MV)is a bioactive,plant-derived triterpenoid known for its various pharmacological activities.However,the i...BACKGROUND High levels of acetaminophen(APAP)consumption can result in significant liver toxicity.Mogroside V(MV)is a bioactive,plant-derived triterpenoid known for its various pharmacological activities.However,the impact of MV on acute liver injury(ALI)is unknown.AIM To investigate the hepatoprotective potential of MV against liver damage caused by APAP and to examine the underlying mechanisms.METHODS Mice were divided into three groups:Saline,APAP and APAP+MV.MV(10 mg/kg)was given intraperitoneally one hour before APAP(300 mg/kg)administration.Twenty-four hours after APAP exposure,serum transaminase levels,liver necrotic area,inflammatory responses,nitrotyrosine accumulation,and c-jun-N-terminal kinase(JNK)activation were assessed.Additionally,we analyzed reactive oxygen species(ROS)levels,JNK activation,and cell death in alpha mouse liver 12(AML12)cells.RESULTS MV pre-treatment in vivo led to a reduction in the rise of aspartate transaminase and alanine transaminase levels,mitigated liver damage,decreased nitrotyrosine accumulation,and blocked JNK phosphorylation resulting from APAP exposure,without affecting glutathione production.Similarly,MV diminished the APAP-induced increase in ROS,JNK phosphorylation,and cell death in vitro.CONCLUSION Our study suggests that MV treatment alleviates APAP-induced ALI by reducing ROS and JNK activation.展开更多
Reactive oxygen species(ROS)are closely related to cell death,proliferation and inflammation.However,excessive ROS levels may exceed the cellular oxidative capacity and cause irreversible damage.Organisms are often in...Reactive oxygen species(ROS)are closely related to cell death,proliferation and inflammation.However,excessive ROS levels may exceed the cellular oxidative capacity and cause irreversible damage.Organisms are often inadvertently exposed to nanomaterials(NMs).Therefore,elucidating the specific routes of ROS generation induced by NMs is crucial for comprehending the toxicity mechanisms of NMs and regulating their potential applications.This paper provides a comprehensive review of the toxicity mechanisms and applications of NMs from three perspectives:(1)Organelle perspective.Investigating the impact of NMmediated ROS onmitochondria,unravelingmechanisms at the organelle level.(2)NMs’perspective.Exploring the broad applications and biosafety considerations of Nanozymes,a unique class of NMs.(3)Cellular system.Examining the toxic effects and mechanisms of NMs in cells at a holistic cellular level.Expanding on these perspectives,the paper scrutinizes the regulation of Fenton reactions by NMs in organisms.Furthermore,it introduces diseases resulting fromNM-mediated ROS at the organism level.This comprehensive review aims to provide valuable insights for studying NM-mediated mechanisms at both cellular and organism levels,offering considerations for the safe design of nanomaterials.展开更多
Advanced oxidation processes are promising for degradation of the highly chemical stability and refractory methylisothiazolinone(MIT) bactericides in relevant industrial wastewater.In order to assemble a low cost and ...Advanced oxidation processes are promising for degradation of the highly chemical stability and refractory methylisothiazolinone(MIT) bactericides in relevant industrial wastewater.In order to assemble a low cost and high performance electrochemical oxidation system for wastewater treatment,granular active carbon(GAC) was decorated by doping Ce,Sn,Sb to synthesize Sn-Sb-Ce/GAC using sol-gel method as particle electrode filled into a three-dimensional(3D) electrochemical reactor.Scanning electron microscopy(SEM),energy-dispersive spectroscopy(EDS) and X-ray diffraction(XRD) experiments revealed that the Sn-Sb-Ce/GAC particle electrode crystal particles were compact and uniform,and the surface structure was improved.The ten cyclic experiments indicated that the Sn-Sb-Ce/GAC particle electrode had high stability and low dissolution of the loaded active substance.The degradation mechanism of MIT was studied under the optimal working conditions of 3D electrode system with GAC of 5 g/L,current density of 20 mA/cm^(2),initial pH 5,electrolyte concentration of Na_(2)SO_(4)0.02 mol/L and reaction time of 120 min.The indirect electrochemical degradation of MIT was dominated by active substance pathway that active chlorine rather than free radicals(·OH) played the main role.Comparing with conventional two-dimensional(2D) electrode system,the 3D electrochemical system has larger active electrode area,higher treatment efficiency and lower energy consumption than the former.The 3D electrochemical system could remove 96.5% of MIT from the actual high-salt reverse osmosis concentrate wastewater in 30 min.It has a certain removal effect on UV_(254)in wastewater,but has a better removal effect on fluorescent substances.This study proposed a new strategy to develop transition metal and rare earth metal particle electrodes using carbon-based materials for high efficient electrocatalytic oxidation in the electrochemical treatment system.展开更多
Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in m...Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remain unclear.In this study,we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line NIL-Rf6 using hematoxylin-eosin staining and 4ʹ,6-diamidino-2-phenylindole(DAPI)staining.HL-CMS is characterized by gametophytic sterility,where the aborted pollen grains are empty,and the tapetal layer remains intact.Transmission electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage,revealing significant mitochondrial alterations,characterized by the formation of'large spherical mitochondria',occurred at the binucleate stage in the YA line.Additionally,metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid pathways.Notably,the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species(ROS)levels.Therefore,we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites,with impaired mitochondrial energy production and reduced flavonoid biosynthesis as the main causes of ROS accumulation and pollen abortion in rice.展开更多
Approximately 30%of the global population struggles with access to potable water,and 60%lacks adequate sanitation.Effective disinfection is crucial,however,heterogeneous systems,despite their benefits,often exhibit lo...Approximately 30%of the global population struggles with access to potable water,and 60%lacks adequate sanitation.Effective disinfection is crucial,however,heterogeneous systems,despite their benefits,often exhibit lower efficacy compared to homogeneous method,presenting a significant challenge[1].In heterogeneous catalysis,photocatalytic disinfection holds immense promise for various applications.However,two key factors significantly impact the efficacy of photocatalytic disinfection:the generation of reactive oxygen species(ROSs)by the photocatalyst and the interaction between ROS and bacteria.展开更多
基金supported by the National Natural Science Foundation of China(61171120)
文摘This paper focuses on the performance analysis of flexible reactive systems. The performance analysis consists of two phases： first system modeling, second performance evalua-tion. The paper models the flexible reactive system by the stochas-tic statecharts method, and uses the simulation method to evalu-ate the performance. To make use of the feature of event-triggered state transitions in the statecharts, a new method of simulation is proposed based on the techniques of the discrete-event system simulation. The new method solves the problem of computer imple-mentation of stochastic events, probabilistic transition, concurrent states, paral el actions, and broadcast communication mechanism in the stochastic statecharts. An example of a flexible manufactur-ing system is presented. The simulation result of the example is consistent with the analytical result, which shows the feasibility of the proposed new simulation method.
基金financial support provided by the National Key R&D Program of China(No.2022YFC2603800)the National Natural Science Foundation of China(No.22274113)。
文摘Reactive oxygen species(ROS)are essential in various pathological and physiological processes.Developing nanosystems that generate ROS in a controlled manner is of great interest for nanomedicine.DNA nanotechnology offers a promising approach to constructing programmable ROS-generating platforms.By incorporating photosensitizers or metal ions,DNA nanostructures can be designed to produce ROS in a spatially and temporally desired fashion.DNA-based ROS-generating nanosystems hold great potential in intracellular homeostasis regulation,drug release,and cancer therapy.This review summarizes recent advances in developing DNA-based ROS-generating nanosystems,highlights their emerging biomedical applications,and discusses the opportunities and challenges for further applications.DNA nanotechnology provides a versatile toolkit to construct biocompatible ROS-generating platforms for next-generation nanomedicines.
基金supported by Fondo Nacional de Desarrollo Científico y Tecnológico(FONDECYT)#1200836,#1210644,and#1240888,and Agencia Nacional de Investigación y Desarrollo(ANID)-FONDAP#15130011(to LL)FONDECYT#3230227(to MFG).
文摘Astrocytes are the most abundant type of glial cell in the central nervous system.Upon injury and inflammation,astrocytes become reactive and undergo morphological and functional changes.Depending on their phenotypic classification as A1 or A2,reactive astrocytes contribute to both neurotoxic and neuroprotective responses,respectively.However,this binary classification does not fully capture the diversity of astrocyte responses observed across different diseases and injuries.Transcriptomic analysis has revealed that reactive astrocytes have a complex landscape of gene expression profiles,which emphasizes the heterogeneous nature of their reactivity.Astrocytes actively participate in regulating central nervous system inflammation by interacting with microglia and other cell types,releasing cytokines,and influencing the immune response.The phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway is a central player in astrocyte reactivity and impacts various aspects of astrocyte behavior,as evidenced by in silico,in vitro,and in vivo results.In astrocytes,inflammatory cues trigger a cascade of molecular events,where nuclear factor-κB serves as a central mediator of the pro-inflammatory responses.Here,we review the heterogeneity of reactive astrocytes and the molecular mechanisms underlying their activation.We highlight the involvement of various signaling pathways that regulate astrocyte reactivity,including the PI3K/AKT/mammalian target of rapamycin(mTOR),αvβ3 integrin/PI3K/AKT/connexin 43,and Notch/PI3K/AKT pathways.While targeting the inactivation of the PI3K/AKT cellular signaling pathway to control reactive astrocytes and prevent central nervous system damage,evidence suggests that activating this pathway could also yield beneficial outcomes.This dual function of the PI3K/AKT pathway underscores its complexity in astrocyte reactivity and brain function modulation.The review emphasizes the importance of employing astrocyte-exclusive models to understand their functions accurately and these models are essential for clarifying astrocyte behavior.The findings should then be validated using in vivo models to ensure real-life relevance.The review also highlights the significance of PI3K/AKT pathway modulation in preventing central nervous system damage,although further studies are required to fully comprehend its role due to varying factors such as different cell types,astrocyte responses to inflammation,and disease contexts.Specific strategies are clearly necessary to address these variables effectively.
基金supported by the Ministry of Education and Science of the Russian Federation(No.2019-0251).
文摘A new gas preparation system(GasPS-RCS)is proposed to solve two tasks:(A)to heat helium gas for tank pressurization;(B)to prepare gas for the Launch Vehicle(LV)Reactive Control System(RCS)at the LV orientation and stabilization sections of the LV on passive parts of the flight trajectory,to provide conditions for launching the Liquid Rocket Engine(LRE).The system includes a gas generator based on hydrogen peroxide,a separator for water separation,heat exchangers independent of the LRE,and gas-jet nozzles.The proposed new system allowed to reduce the length of pressurizing gas lines and reduce the increased helium gas consumption during the heat exchanger warm-up interval of the LRE during its launch.A special advantage of the proposed system is the possibility of ground testing of heat exchangers without an operating LRE.A mathematical model based on the first law of thermodynamics was used to perform a comparative analysis of GasPS-RCS with traditional pressurization and RCS systems.To validate the mathematical model,the experimental studies of helium pressurizing of a liquid nitrogen tank were conducted.The results show that the deviation of experimental and calculated values for pressure is 1.1%and for temperature up to 2%.According to the results of comparative analysis,the GasPS-RCS is 259 kg lighter for the first stage and 80 kg lighter for the second stage of the LV.
基金funded by the“Research and Application Project of Collaborative Optimization Control Technology for Distribution Station Area for High Proportion Distributed PV Consumption(4000-202318079A-1-1-ZN)”of the Headquarters of the State Grid Corporation.
文摘Considering the uncertainty of grid connection of electric vehicle charging stations and the uncertainty of new energy and residential electricity load,a spatio-temporal decoupling strategy of dynamic reactive power optimization based on clustering-local relaxation-correction is proposed.Firstly,the k-medoids clustering algorithm is used to divide the reduced power scene into periods.Then,the discrete variables and continuous variables are optimized in the same period of time.Finally,the number of input groups of parallel capacitor banks(CB)in multiple periods is fixed,and then the secondary static reactive power optimization correction is carried out by using the continuous reactive power output device based on the static reactive power compensation device(SVC),the new energy grid-connected inverter,and the electric vehicle charging station.According to the characteristics of the model,a hybrid optimization algorithm with a cross-feedback mechanism is used to solve different types of variables,and an improved artificial hummingbird algorithm based on tent chaotic mapping and adaptive mutation is proposed to improve the solution efficiency.The simulation results show that the proposed decoupling strategy can obtain satisfactory optimization resultswhile strictly guaranteeing the dynamic constraints of discrete variables,and the hybrid algorithm can effectively solve the mixed integer nonlinear optimization problem.
基金supported by the National Natural Science Foundation of China(Grant No.12172052)the Foundation of State Key Laboratory of Explosion Science and Safety Protection(Grant No.QKKT24-02).
文摘The penetration-deflagration coupling damage performance of rod-like reactive shaped charge pene-trator(RRSCP)impacting thick steel plates is investigated by theoretical analysis and experiments.A penetration-deflagration coupling damage model is developed to predict the penetration depth and cratering diameter.Four type of aluminum-polytetrafluoroethylene-copper(Al-PTFE-Cu)reactive liners with densities of 2.3,2.7,3.5,and 4.5 g·cm^(-3) are selected to conduct the penetration experiments.The comparison results show that model predictions are in good agreement with the experimental data.By comparing the penetration depth and cratering diameter in the inert penetration mode and the penetration-deflagration coupling mode,the influence mechanism that the penetration-induced chemical response is unfavorable to penetration but has an enhanced cratering effect is revealed.From the formation characteristics,penetration effect and penetration-induced chemical reaction be-haviors,the influence of reactive liner density on the penetration-deflagration performance is further analyzed.The results show that increasing the density of reactive liner significantly increases both the kinetic energy and length of the reactive penetrator,meanwhile effectively reduces the weakened effect of penetration-induced chemical response,resulting in an enhanced penetration capability.However,due to the decreased diameter and potential energy content of reactive penetrator,the cratering capa-bility is weakened significantly.
基金supported by the National Research Foundation of Korea (NRF)funded by the Ministry of Science,ICT&Future Planning (2022R1A2C2006229,2022R1A6A3A01086868)Korea Dementia Research Project through the Korea Dementia Research Center (KDRC)funded by the Ministry of Health&Welfare and Ministry of Science and ICT,Republic of Korea (RS-2024-00345328)KIST Institutional Grant (2E32851)。
文摘Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder characterized by cognitive impairment and distinct neuropathological features,including amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis.Developing effective diagnostic,preventative,and therapeutic strategies for AD necessitates the establishment of animal models that accurately recapitulate the pathophysiological processes of the disease.Existing transgenic mouse models have significantly contributed to understanding AD pathology but often fail to replicate the complexity of human AD.Additionally,these models are limited in their ability to elucidate the interplay among amyloid-βplaques,neurofibrillary tangles,and reactive astrogliosis due to the absence of spatially and temporally specific genetic manipulation.In this study,we introduce a novel AD mouse model(APP/PS1-TauP301L-Adeno mice)designed to rapidly induce pathological symptoms and enhance understanding of AD mechanisms.Neurofibrillary tangles and severe reactive astrogliosis were induced by injecting AAVDJ-EF1a-hTauP301L-EGFP and Adeno-GFAP-GFP viruses into the hippocampi of 5-month-old APP/PS1 mice.Three months post-injection,these mice exhibited pronounced astrogliosis,substantial amyloid-βplaque accumulation,extensiveneurofibrillarytangles,accelerated neuronal loss,elevated astrocytic GABA levels,and significant spatial memory deficits.Notably,these pathological features were less severe in AAVTauP301L-expressing APP/PS1 mice without augmented reactive astrogliosis.These findings indicate an exacerbating role of severe reactive astrogliosis in amyloid-βplaque and neurofibrillary tangle-associated pathology.The APP/PS1-TauP301L-Adeno mouse model provides a valuable tool for advancing therapeutic research aimed at mitigating the progression of AD.
基金National Natural Science Foundation of China(12002045)Supported by State Key Laboratory of Explosion Science and Safety Protection,Beijing Institute of Technology(QNKT22-09)。
文摘A high-density tungsten-zirconium-titanium(W-Zr-Ti)reactive alloy was prepared by powder metallurgy.This alloy exhibits high density,high strength,and violent energy release characteristics,resulting in outstanding penetration and ignition abilities.Dynamic impact experiment demonstrated its strain rate hardening effect,and the energetic characteristics were investigated by digital image processing technique and thermal analysis experiment.The results show that W-Zr-Ti reactive alloy performs compressive strength of 2.25 GPa at 5784 s^(-1)strain rate,and its exothermic reaction occurs at about 961 K.Based on the explosion test and shock wave theory,thresholds of enhanced damage effect are less than 35.77 GPa and 5.18×10^(4)kJ/m^(2)for shock pressure and energy,respectively.Furthermore,the transformation of fracture behavior and failure mechanism is revealed,which causes the increase in compressive strength and reaction intensity under dynamic loading.
文摘Reactive oxygen species(ROS),including singlet oxygen(^(1)O_(2)),hydroxyl radicals(·OH),and superoxide anions(O_(2)^(·-)),are highly reactive molecules that play central roles in many chemical,biological,and environmental processes due to their strong oxidative power[1].Generating ROS in a controlled manner under mild conditions is essential for achieving selective oxidation reactions.Light-driven methods are especially appealing for this purpose,as they offer precise control over where and when ROS are produced.
基金Youth Foundation of Hebei Province Department of Education Fund,China(No.QN2023090)Opening Project of Textile Ecological Dyeing and Finishing Key Laboratory of Sichuan Province(Chengdu Textile College),China(No.2024DF-AO2)Innovation and Entrepreneurship Training Program for College Students,China(No.202410082023)。
文摘Aiming to solve the problem of large discharge and severe pollution of reactive dyeing wastewater for wool fabrics,peroxodisulfate(SPS)was used for the degradation and recycling of dyeing wastewater containing reactive dye Lanasol Red CE.The process of degrading the reactive dye was determined by using the dye residual rate as the evaluation index.The feasibility of reactive dyeing of wool fabrics using recycled dyeing wastewater was confirmed by measuring the dye uptake,exhaustion and fixation rates,as well as color parameters and fastness of the dyed fabrics.The results showed that the appropriate conditions for degrading Lanasol Red CE were 0.2 g/L SPS,an initial pH value of 3 and 100℃for 30 min.Under these conditions,the dye degradation rate was as high as 93.14%.When the recycled dyeing wastewater was used for dyeing of wool fabrics,the exhaustion rate of Lanasol Red CE exceeded 99%,and the fixation rate was higher than that achieved by the conventional dyeing process.Under the same dyeing conditions,the recycled-dyed fabrics appeared darker.When the number of cycles was fewer than five,the effect on color fastness was not obvious.Although the color fastness to rubbing and washing of the fabrics dyed in the 10th cycle decreased by half a grade and 1 grade,respectively,compared to that of the fabrics dyed with the conventional dyeing process,they still met the production requirements.
文摘BACKGROUND Colon cancer is one of the most common malignancies worldwide,and chemo-therapy is a widely used strategy in colon cancer clinical therapy.Chemotherapy resistance is the main cause of recurrence and progression in colon cancer.Thus,novel drugs for treatment are urgently needed.Tetramethylpyrazine(TMP),a component of the traditional Chinese medicine Chuanxiong Hort,has been proven to exhibit a beneficial effect in tumors.AIM To investigate the potential anticancer activity of TMP in colon cancer and the underlying mechanisms.METHODS Colon cancer cells were incubated with different concentrations of TMP.Cell viability was evaluated by crystal violet staining assay,and cell apoptosis was assessed by flow cytometry.Apoptosis-associated protein expression was measured using Western blot analysis.Intracellular reactive oxygen species(ROS)levels were assessed by flow cytometry using DCF fluorescence intensity.Xeno-grafts were established by the subcutaneous injection of colon cancer cells into nude mice;tumor growth was monitored and intracellular ROS was detected in tumors by malondialdehyde assay.RESULTS TMP induced apoptosis of colon cancer cells via the activation of the mitochon-drial pathway.TMP increased the generation of intracellular ROS and triggered mitochondria-mediated apoptosis in a caspase-dependent manner.CONCLUSION Our study demonstrates that TMP induces the apoptosis of colon cancer cells and increases the generation of intracellular ROS.TMP triggers mitochondria-mediated apoptosis in a caspase-dependent manner.The accumu-lation of intracellular ROS is involved in TMP-induced apoptosis.Our findings suggest that TMP may be a potential therapeutic drug for the treatment of colon cancer.
基金National Natural Science Foundation of China(U23A20199)Yunnan Revitalization Talent Support Program“Yunling Scholar”and Yunnan Fundamental Research Projects(202201AS070056)。
文摘Ca^(2+)signaling plays crucial roles in plant stress responses,including defense against insects.To counteract insect feeding,different parts of a plant deploy systemic signaling to communicate and coordinate defense responses,but little is known about the underlying mechanisms.In this study,micrografting,in vivo imaging of Ca^(2+)and reactive oxygen species(ROS),quantification of jasmonic acid(JA)and defensive metabolites,and bioassay were used to study how Arabidopsis seedlings regulate systemic responses in leaves after hypocotyls are wounded.We show that wounding hypocotyls rapidly activated both Ca^(2+)and ROS signals in leaves.RBOHD,which functions to produce ROS,along with two glutamate receptors GLR3.3 and GLR3.6,but not individually RBOHD or GLR3.3 and GLR3.6,in hypocotyls regulate the dynamics of systemic Ca^(2+)signals in leaves.In line with the systemic Ca^(2+)signals,after wounding hypocotyl,RBOHD,GLR3.3,and GLR3.6 in hypocotyl also cooperatively regulate the transcriptome,hormone jasmonic acid,and defensive secondary metabolites in leaves of Arabidopsis seedlings,thus controlling the systemic resistance to insects.Unlike leaf-to-leaf systemic signaling,this study reveals the unique regulation of wounding-induced hypocotyl-to-leaf systemic signaling and sheds new light on how different plant organs use complex signaling pathways to modulate defense responses.
文摘The electrokinetic(EK)process has been proposed for soil decontamination from heavy metals and organic matter.The advantages of the EK process include the low operating energy,suitability for fine-grained soil decontamination,and no need for excavation.During the last three decades,enhanced and hybrid EK systems were developed and tested for improving the efficiency of contaminants removal from soils.Chemically enhanced-EK processes exhibited excellent efficiency in removing contaminants by controlling the soil pH or the chemical reaction of contaminants.EK hybrid systems were tested to overcome environmental hurdles or technical drawbacks of decontamination technologies.Hybridization of the EK process with phytoremediation,bioremediation,or reactive filtermedia(RFM)improved the remediation process performance by capturing contaminants or facilitating biological agents’movement in the soil.Also,EK process coupling with solar energy was proposed to treat off-grid contaminated soils or reduce the EK energy requirements.This study reviews recent advancements in the enhancement and hybrid EK systems for soil remediation and the type of contaminants targeted by the process.The study also covered the impact of operating parameters,imperfect pollution separation,and differences in the physicochemical characteristics and microstructure of soil/sediment on the EK performance.Finally,a comparison between various remediation processes was presented to highlight the pros and cons of these technologies.
文摘In the current study novel magnetic BiOBr-Gd^(3+)doped CoFe_(2)O_(4)heterojunction nanocomposites fabricated by chemical precipitation method for Acid Violet 7(AV 7)and Reactive Red 120(RR 120)dye degradation under sunlight are presented.The samples were well characterized by X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM),scanning electron microscopy(SEM),energy dispersive X-ray analysis(EDAX),UV-visible near infrared(NIR),photoluminescence(PL),TRF,EIS,Mott-Schottky,and BET analysis.X-ray diffractograms of BiOBr-Gd^(3+)doped CoFe_(2)O_(4)nanocomposites authenticate the formation of the composite composed of diffraction peaks of both BiOBr and Gd^(3+)doped CoFe_(2)O_(4)samples.Photoluminescence(PL),Time Resolved Fluorescence Spectrocopy(TRF)and Electrochemical Impedance Spectroscopy(EIS)investigations were explored to examine the transfer efficiency of photoinduced charge carriers.Among the synthesized materials,the BiOBr-Gd^(3+)doped CoFe_(2)O_(4)-2 nanocomposite presents superior degradation capability for RR 120 of 87.34%and AV 7 dyes of 98.41%.The apparent rate constant(k)value of 0.1317 min^(-1)for AV 7 is sixteen times higher and RR 120(0.0135 min^(-1))is 2 times higher than pristine BiOBr.The radical trapping experiments also proved the role of photogenerated h^(+),O_(2)·^(-),and·OH radicals in the degradation mechanism.The superior performance of BiOBr-Gd^(3+)doped CoFe_(2)O_(4)-2 is ascribed to the formation of the heterostructure,the in-built electric field,and energy-band alignment,which thermodynamically and kinetically favors the photoactivity and stability of the heterojunction.
基金financially supported by the National Postdoctoral Program for Innovative Talents, China (No. BX2021327)the National Natural Science Foundation of China (Nos. 22206194 and U2267222)+1 种基金the Ningbo Natural Science Foundation of China (No. 2023J337)the Yongjiang Talent Introduction Programme, China (No. 2 021A-161-G)。
文摘Herein, the electrochemical behaviors of Sr on inert W electrode and reactive Zn/Al electrodes were systematically investig-ated in LiCl–KCl–SrCl2molten salts at 773 K using various electrochemical methods. The chemical reaction potentials of Li and Sr on re-active Zn/Al electrodes were determined. We observed that Sr could be extracted by decreasing the activity of the deposited metal Sr onthe reactive electrode, although the standard reduction potential of Sr(II)/Sr was more negative than that of Li(I)/Li. The electrochemicalextraction products of Sr on reactive Zn and Al electrodes were Zn13Sr and Al4Sr, respectively, with no codeposition of Li observed.Based on the density functional theory calculations, both Zn13Sr and Al4Sr were identified as stable intermetallic compounds with Zn-/Al-rich phases. In LiCl–KCl molten salt containing 3wt% SrCl2, the coulombic efficiency of Sr in the Zn electrode was ~54%. The depolar-ization values for Sr on Zn and Al electrodes were 0.864 and 0.485 V, respectively, exhibiting a stronger chemical interaction between Znand Sr than between Al and Sr. This study suggests that using reactive electrodes can facilitate extraction of Sr accumulated while elec-trorefining molten salts, thereby enabling the purification and reuse of the salt and decreasing the volume of the nuclear waste.
基金Supported by Guangxi Natural Science Foundation of China,No.2024GXNSFAA010040Special Fund of the Central Government Guiding Local Scientific and Technological Development by Guangxi Science and Technology Department,No.GuikeZY21195024National Natural Science Foundation of China,No.82260499 and No.82460463.
文摘BACKGROUND High levels of acetaminophen(APAP)consumption can result in significant liver toxicity.Mogroside V(MV)is a bioactive,plant-derived triterpenoid known for its various pharmacological activities.However,the impact of MV on acute liver injury(ALI)is unknown.AIM To investigate the hepatoprotective potential of MV against liver damage caused by APAP and to examine the underlying mechanisms.METHODS Mice were divided into three groups:Saline,APAP and APAP+MV.MV(10 mg/kg)was given intraperitoneally one hour before APAP(300 mg/kg)administration.Twenty-four hours after APAP exposure,serum transaminase levels,liver necrotic area,inflammatory responses,nitrotyrosine accumulation,and c-jun-N-terminal kinase(JNK)activation were assessed.Additionally,we analyzed reactive oxygen species(ROS)levels,JNK activation,and cell death in alpha mouse liver 12(AML12)cells.RESULTS MV pre-treatment in vivo led to a reduction in the rise of aspartate transaminase and alanine transaminase levels,mitigated liver damage,decreased nitrotyrosine accumulation,and blocked JNK phosphorylation resulting from APAP exposure,without affecting glutathione production.Similarly,MV diminished the APAP-induced increase in ROS,JNK phosphorylation,and cell death in vitro.CONCLUSION Our study suggests that MV treatment alleviates APAP-induced ALI by reducing ROS and JNK activation.
基金supported by the National Natural Science Foundation of China(No.22176206).
文摘Reactive oxygen species(ROS)are closely related to cell death,proliferation and inflammation.However,excessive ROS levels may exceed the cellular oxidative capacity and cause irreversible damage.Organisms are often inadvertently exposed to nanomaterials(NMs).Therefore,elucidating the specific routes of ROS generation induced by NMs is crucial for comprehending the toxicity mechanisms of NMs and regulating their potential applications.This paper provides a comprehensive review of the toxicity mechanisms and applications of NMs from three perspectives:(1)Organelle perspective.Investigating the impact of NMmediated ROS onmitochondria,unravelingmechanisms at the organelle level.(2)NMs’perspective.Exploring the broad applications and biosafety considerations of Nanozymes,a unique class of NMs.(3)Cellular system.Examining the toxic effects and mechanisms of NMs in cells at a holistic cellular level.Expanding on these perspectives,the paper scrutinizes the regulation of Fenton reactions by NMs in organisms.Furthermore,it introduces diseases resulting fromNM-mediated ROS at the organism level.This comprehensive review aims to provide valuable insights for studying NM-mediated mechanisms at both cellular and organism levels,offering considerations for the safe design of nanomaterials.
基金the financial supports from Major Science and Technology project of China Power Engineering Consulting Group Co., Ltd. "Research on Green and digital Intelligent Technology of Sewage Treatment Plant" (No. CEEC2023-ZDYF-09)Technology Innovation Ability Improvement Project of Shandong Province, China (No. 2022TSGC1247)。
文摘Advanced oxidation processes are promising for degradation of the highly chemical stability and refractory methylisothiazolinone(MIT) bactericides in relevant industrial wastewater.In order to assemble a low cost and high performance electrochemical oxidation system for wastewater treatment,granular active carbon(GAC) was decorated by doping Ce,Sn,Sb to synthesize Sn-Sb-Ce/GAC using sol-gel method as particle electrode filled into a three-dimensional(3D) electrochemical reactor.Scanning electron microscopy(SEM),energy-dispersive spectroscopy(EDS) and X-ray diffraction(XRD) experiments revealed that the Sn-Sb-Ce/GAC particle electrode crystal particles were compact and uniform,and the surface structure was improved.The ten cyclic experiments indicated that the Sn-Sb-Ce/GAC particle electrode had high stability and low dissolution of the loaded active substance.The degradation mechanism of MIT was studied under the optimal working conditions of 3D electrode system with GAC of 5 g/L,current density of 20 mA/cm^(2),initial pH 5,electrolyte concentration of Na_(2)SO_(4)0.02 mol/L and reaction time of 120 min.The indirect electrochemical degradation of MIT was dominated by active substance pathway that active chlorine rather than free radicals(·OH) played the main role.Comparing with conventional two-dimensional(2D) electrode system,the 3D electrochemical system has larger active electrode area,higher treatment efficiency and lower energy consumption than the former.The 3D electrochemical system could remove 96.5% of MIT from the actual high-salt reverse osmosis concentrate wastewater in 30 min.It has a certain removal effect on UV_(254)in wastewater,but has a better removal effect on fluorescent substances.This study proposed a new strategy to develop transition metal and rare earth metal particle electrodes using carbon-based materials for high efficient electrocatalytic oxidation in the electrochemical treatment system.
基金supported by the National Natural Science Foundation of China(Grant No.32472185)the Key Research and Development Program of Hubei Province,China(Grant No.2022BFE003)the Hubei Agriculture Science and Technology Innovation Center program,and the National Rice Industry Technology System,China(Grant No.CARS-01-07).
文摘Honglian type-cytoplasmic male sterility(HL-CMS)is caused by the inter-communication between the nucleus and mitochondria.However,the mechanisms by which sterility genes regulate metabolic alterations and changes in mitochondrial morphology in the pollen of HL-CMS remain unclear.In this study,we compared the morphological differences between the pollen of the male sterile line YA and the near-isogenic line NIL-Rf6 using hematoxylin-eosin staining and 4ʹ,6-diamidino-2-phenylindole(DAPI)staining.HL-CMS is characterized by gametophytic sterility,where the aborted pollen grains are empty,and the tapetal layer remains intact.Transmission electron microscopy was employed to observe mitochondrial morphological changes at the microspore stage,revealing significant mitochondrial alterations,characterized by the formation of'large spherical mitochondria',occurred at the binucleate stage in the YA line.Additionally,metabolomics analysis revealed decreased levels of metabolites associated with the carbohydrate and flavonoid pathways.Notably,the decrease in flavonoids was found to contribute to an elevation in reactive oxygen species(ROS)levels.Therefore,we propose a model in which rice fertility is modulated by the levels of pollen carbohydrates and flavonoid metabolites,with impaired mitochondrial energy production and reduced flavonoid biosynthesis as the main causes of ROS accumulation and pollen abortion in rice.
基金supported by the Taishan Scholar Project of Shandong Province(tsqn202211168)the National Natural Science Foundation of China(52422213,52272212)the Natural Science Foundation of Shandong Province(ZR2022JQ20).
文摘Approximately 30%of the global population struggles with access to potable water,and 60%lacks adequate sanitation.Effective disinfection is crucial,however,heterogeneous systems,despite their benefits,often exhibit lower efficacy compared to homogeneous method,presenting a significant challenge[1].In heterogeneous catalysis,photocatalytic disinfection holds immense promise for various applications.However,two key factors significantly impact the efficacy of photocatalytic disinfection:the generation of reactive oxygen species(ROSs)by the photocatalyst and the interaction between ROS and bacteria.
