Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino...Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino acids(LAA)on Ca digestibility,absorption and reabsorption in pigs fed with VLP diets.Forty-eight piglets were assigned to six treatments:positive control(PC),negative control(NC),and NC containing LAA 25%,LAA 50%,LAA+BCAA 25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Results:Relative to PC or NC,LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus(P),but lower plasma vitamin D3.LB50 tended to increase vitamin D receptor transcript and protein in the gut,but decreased mRNA or protein abundance of parathyroid hormone 1 receptor(PTH1R),calbindin 1(CALB1),cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine.LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5,CALB1 and solute carrier family 17 member 4 in kidney.Conclusion:Overall,BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.展开更多
BACKGROUND Conservative treatments have been reported to diminish or resolve clinical symptoms of lumbar intervertebral disc herniation(LIDH)within a few weeks.CASE SUMMARY Computed tomography and magnetic resonance i...BACKGROUND Conservative treatments have been reported to diminish or resolve clinical symptoms of lumbar intervertebral disc herniation(LIDH)within a few weeks.CASE SUMMARY Computed tomography and magnetic resonance imaging(MRI)of the lumbar region of a 25-yearold male diagnosed with LIDH showed prolapse of the L5/S2 disc.The disc extended 1.0 cm beyond the vertebral edge and hung along the posterior vertebral edge.The patient elected a conservative treatment regimen that included traditional Chinese medicine(TCM),acupuncture,and massage.During a follow-up period of more than 12 mo,good improvement in pain was reported without complications.MRI of the lumbar region after 12 mo showed obvious reabsorption of the herniation.CONCLUSION A conservative treatment regimen of TCM,acupuncture,and massage promoted reabsorption of a prolapsed disc.展开更多
In this editorial,we discussed the article published in the recent issue of the World Journal of Diabetes.To understand the effect of mizagliflozin on kidney injury induced by diabetes,we focused on the mechanisms by ...In this editorial,we discussed the article published in the recent issue of the World Journal of Diabetes.To understand the effect of mizagliflozin on kidney injury induced by diabetes,we focused on the mechanisms by which high glucose triggers oxidative stress and contributes to kidney injury in diabetes.The high level of unmetabolized glucose reaching the kidney triggers glucose reabsorption by renal tubules,which elevates the cellular glucose level of renal cells.High glucose induces lactate dehydrogenase overexpression and thus shifts glucose metabolism,which causes mitochondrial dysfunction.Mitochondria generate approximately 90%of the reactive oxygen species in cells,whose dysfunction further alters glucose metabolism and enhances reactive oxygen species generation.Oxidative stress stimulates proinflammatory factor production and kidney inflammatory injury.Mizagliflozin decreases glucose reabsorption and thus ameliorates diabetes-induced kidney injury.展开更多
目的观察中药复方通心络对自发性2型糖尿病KK-Ay小鼠肾小管重吸收功能及肾组织megalin和cubilin的影响,探讨中药复方防治糖尿病肾病的作用机制。方法采用KK-Ay小鼠建立糖尿病肾病模型,随机分为模型组、通心络组、缬沙坦组各15只,设C57BL...目的观察中药复方通心络对自发性2型糖尿病KK-Ay小鼠肾小管重吸收功能及肾组织megalin和cubilin的影响,探讨中药复方防治糖尿病肾病的作用机制。方法采用KK-Ay小鼠建立糖尿病肾病模型,随机分为模型组、通心络组、缬沙坦组各15只,设C57BL/6J小鼠15只为正常组,给药干预12周后,测定空腹血糖(FPG)、24 h尿微量白蛋白排泄率(UAER)、尿α_1-微球蛋白(α_1-MG)、β_2微球蛋白(β_2-MG)、尿N-乙酰-β-D-葡萄糖苷酶(NAG)含量,采用Western Blot及Realtime PCR方法分别检测肾组织megalin和cubilin的蛋白及基因表达水平。结果与正常组比较,模型组24 h UAER、α_1-MG、β_2-MG、NAG均明显升高(P<0.05),肾组织megalin和cubilin的蛋白和基因表达明显降低(P<0.05);与模型组比较,用药组FPG无明显变化(P>0.05),但24 h UAER、α_1-MG、β_2-MG、NAG等含量明显降低(P<0.05),肾组织megalin和cubilin的蛋白和基因表达上调(P<0.05)。结论通心络可减少2型糖尿病小鼠尿蛋白,改善肾小管重吸收功能,其作用机制可能与上调肾组织中的megalin和cubilin的表达有关。展开更多
基金funded by Animal Health and Production and Animal Products:Improved Nutritional Performance,Growth,and Lactation of Animals,grant no. 2021–67016-33399/project accession no. 1024334Hatch project accession no. 1012889 from the USDA National Institute of Food and Agriculture (NIFA)
文摘Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino acids(LAA)on Ca digestibility,absorption and reabsorption in pigs fed with VLP diets.Forty-eight piglets were assigned to six treatments:positive control(PC),negative control(NC),and NC containing LAA 25%,LAA 50%,LAA+BCAA 25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Results:Relative to PC or NC,LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus(P),but lower plasma vitamin D3.LB50 tended to increase vitamin D receptor transcript and protein in the gut,but decreased mRNA or protein abundance of parathyroid hormone 1 receptor(PTH1R),calbindin 1(CALB1),cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine.LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5,CALB1 and solute carrier family 17 member 4 in kidney.Conclusion:Overall,BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.
基金Supported by National Nature Science Foundation of China,No.82004495Natural Science Foundation of Shandong Province,China,No.ZR2020QH318+1 种基金The 69th batch of a grant from China Postdoctoral Foundation,No.2021M691985Taishan Scholars Young Experts Program,China,No.tsqn202211349.
文摘BACKGROUND Conservative treatments have been reported to diminish or resolve clinical symptoms of lumbar intervertebral disc herniation(LIDH)within a few weeks.CASE SUMMARY Computed tomography and magnetic resonance imaging(MRI)of the lumbar region of a 25-yearold male diagnosed with LIDH showed prolapse of the L5/S2 disc.The disc extended 1.0 cm beyond the vertebral edge and hung along the posterior vertebral edge.The patient elected a conservative treatment regimen that included traditional Chinese medicine(TCM),acupuncture,and massage.During a follow-up period of more than 12 mo,good improvement in pain was reported without complications.MRI of the lumbar region after 12 mo showed obvious reabsorption of the herniation.CONCLUSION A conservative treatment regimen of TCM,acupuncture,and massage promoted reabsorption of a prolapsed disc.
基金Supported by The Basic Research Project of Wenzhou Municipal Science and Technology Bureau,No.Y20240008The Medical Health Science and Technology Project of Zhejiang Provincial Health Commission,No.2024KY138The Key Laboratory of School of Laboratory Medicine and Life Sciences,Wenzhou Medical University of China,No.JS2023003。
文摘In this editorial,we discussed the article published in the recent issue of the World Journal of Diabetes.To understand the effect of mizagliflozin on kidney injury induced by diabetes,we focused on the mechanisms by which high glucose triggers oxidative stress and contributes to kidney injury in diabetes.The high level of unmetabolized glucose reaching the kidney triggers glucose reabsorption by renal tubules,which elevates the cellular glucose level of renal cells.High glucose induces lactate dehydrogenase overexpression and thus shifts glucose metabolism,which causes mitochondrial dysfunction.Mitochondria generate approximately 90%of the reactive oxygen species in cells,whose dysfunction further alters glucose metabolism and enhances reactive oxygen species generation.Oxidative stress stimulates proinflammatory factor production and kidney inflammatory injury.Mizagliflozin decreases glucose reabsorption and thus ameliorates diabetes-induced kidney injury.
文摘目的观察中药复方通心络对自发性2型糖尿病KK-Ay小鼠肾小管重吸收功能及肾组织megalin和cubilin的影响,探讨中药复方防治糖尿病肾病的作用机制。方法采用KK-Ay小鼠建立糖尿病肾病模型,随机分为模型组、通心络组、缬沙坦组各15只,设C57BL/6J小鼠15只为正常组,给药干预12周后,测定空腹血糖(FPG)、24 h尿微量白蛋白排泄率(UAER)、尿α_1-微球蛋白(α_1-MG)、β_2微球蛋白(β_2-MG)、尿N-乙酰-β-D-葡萄糖苷酶(NAG)含量,采用Western Blot及Realtime PCR方法分别检测肾组织megalin和cubilin的蛋白及基因表达水平。结果与正常组比较,模型组24 h UAER、α_1-MG、β_2-MG、NAG均明显升高(P<0.05),肾组织megalin和cubilin的蛋白和基因表达明显降低(P<0.05);与模型组比较,用药组FPG无明显变化(P>0.05),但24 h UAER、α_1-MG、β_2-MG、NAG等含量明显降低(P<0.05),肾组织megalin和cubilin的蛋白和基因表达上调(P<0.05)。结论通心络可减少2型糖尿病小鼠尿蛋白,改善肾小管重吸收功能,其作用机制可能与上调肾组织中的megalin和cubilin的表达有关。
