With the acceleration of life pace and the increase of environmental stress,skin aging has become a major concern.Traditional anti-aging methods,such as laser therapy and injections,are effective but have limitations ...With the acceleration of life pace and the increase of environmental stress,skin aging has become a major concern.Traditional anti-aging methods,such as laser therapy and injections,are effective but have limitations including side effects,complex procedures,high costs,and long recovery periods.In contrast,a topical cellular re-energizing technology using a combination system of 1%Spherical PDRN(Polydeoxyribonucleotide,a registered product name with the following composition:55%mannitol,30%trehalose,10%sodium DNA,and 5%sodium hyaluronate)and 1%Spherical Collagen(a registered product name with the following composition:55%mannitol,30%trehalose,10%collagen,and 5%sodium hyaluronate)stands out with its natural,convenient,non-invasive,and long-lasting advantages.It fundamentally addresses skin aging and provides consumers with a safer,more comfortable,and comprehensive anti-aging experience.This study prepared a combination system of Spherical PDRN and Collagen via a matrix incorporation method.It investigated mitochondrial function and morphology changes in H_(2)O_(2)-stimulated fibroblasts by measuring mitochondrial membrane potential(a key function indicator),ATP content(reflecting energy production),and mitochondrial morphology(related to cell state and stress response).These analyses provided insights into fibroblast energy metabolism and cellular mechanisms.Additionally,a precise method assessed UVA-induced NAD⁺concentration changes in fibroblasts,reflecting metabolic and antioxidant states under stress.In H_(2)O_(2)-stimulated fibroblasts,the combination system of Spherical PDRN and Spherical Collagen at a concentration of 2.5%significantly increased the average value of mitochondrial membrane potential(polymer/monomer ratio)by 448.28%,and the content of ATP by 108.06%.The mitochondrial morphology in cells appeared as elongated rods,with normal fusion and fission occurring.In UVA-irradiated fibroblasts,the combination system of Spherical PDRN and Spherical Collagen at a concentration of 2.5%significantly increased the concentration of NAD^(+) per unit protein by 35.93%.This study investigated how the combination system of Spherical PDRN and Collagen affects mitochondrial function in fibroblasts.It was found that this system significantly boosts mitochondrial membrane potential,implying a marked rise in mitochondrial activity and energy conversion efficiency.This suggests optimized respiratory chain function and energy metabolism,which are vital for normal cellular functions and stress responses.Moreover,the system significantly raises intracellular ATP levels,directly reflecting enhanced mitochondrial energy production.This not only promotes cellular energy metabolism but also boosts cell vitality and stress resistance.Additionally,the system improves mitochondrial morphology,which is crucial for maintaining mitochondrial dynamics and function.展开更多
文摘With the acceleration of life pace and the increase of environmental stress,skin aging has become a major concern.Traditional anti-aging methods,such as laser therapy and injections,are effective but have limitations including side effects,complex procedures,high costs,and long recovery periods.In contrast,a topical cellular re-energizing technology using a combination system of 1%Spherical PDRN(Polydeoxyribonucleotide,a registered product name with the following composition:55%mannitol,30%trehalose,10%sodium DNA,and 5%sodium hyaluronate)and 1%Spherical Collagen(a registered product name with the following composition:55%mannitol,30%trehalose,10%collagen,and 5%sodium hyaluronate)stands out with its natural,convenient,non-invasive,and long-lasting advantages.It fundamentally addresses skin aging and provides consumers with a safer,more comfortable,and comprehensive anti-aging experience.This study prepared a combination system of Spherical PDRN and Collagen via a matrix incorporation method.It investigated mitochondrial function and morphology changes in H_(2)O_(2)-stimulated fibroblasts by measuring mitochondrial membrane potential(a key function indicator),ATP content(reflecting energy production),and mitochondrial morphology(related to cell state and stress response).These analyses provided insights into fibroblast energy metabolism and cellular mechanisms.Additionally,a precise method assessed UVA-induced NAD⁺concentration changes in fibroblasts,reflecting metabolic and antioxidant states under stress.In H_(2)O_(2)-stimulated fibroblasts,the combination system of Spherical PDRN and Spherical Collagen at a concentration of 2.5%significantly increased the average value of mitochondrial membrane potential(polymer/monomer ratio)by 448.28%,and the content of ATP by 108.06%.The mitochondrial morphology in cells appeared as elongated rods,with normal fusion and fission occurring.In UVA-irradiated fibroblasts,the combination system of Spherical PDRN and Spherical Collagen at a concentration of 2.5%significantly increased the concentration of NAD^(+) per unit protein by 35.93%.This study investigated how the combination system of Spherical PDRN and Collagen affects mitochondrial function in fibroblasts.It was found that this system significantly boosts mitochondrial membrane potential,implying a marked rise in mitochondrial activity and energy conversion efficiency.This suggests optimized respiratory chain function and energy metabolism,which are vital for normal cellular functions and stress responses.Moreover,the system significantly raises intracellular ATP levels,directly reflecting enhanced mitochondrial energy production.This not only promotes cellular energy metabolism but also boosts cell vitality and stress resistance.Additionally,the system improves mitochondrial morphology,which is crucial for maintaining mitochondrial dynamics and function.
