Numerous investigations have uncovered the outstanding therapeutic effect of tea extract on ulcerative colitis(UC).However,the therapeutic potential on UC of Pu’er raw tea extract(PRTE)remains debated and need to be ...Numerous investigations have uncovered the outstanding therapeutic effect of tea extract on ulcerative colitis(UC).However,the therapeutic potential on UC of Pu’er raw tea extract(PRTE)remains debated and need to be investigated.In our study,PRTE(100 and 300 mg/kg)was applied to the DSS-induced colitis mice via intra-gastric administration.Results have shown that PRTE treatment significantly ameliorated DSS-induced weight loss,loose stool,hematochezia,colon atrophy,splenomegaly and colon pathological injury.It could also inhibit UC by down-regulating pro-inflammatory cytokines(IL-1β,IL-6 and TNF-α)and increasing the expression of tight junction protein(zonula occludens-1,ZO-1)to reinforce intestinal barrier.Fecal microbiota transplantation has further confirmed that PRTE could maintain intestinal homeostasis and relieve UC by improve the abundance of beneficial bacteria such as Lactobacillus,Muribaculum,Lachnospiraceae and Rikenellaceae,and reduce the abun-dance of harmful bacteria such as Turicibacter and Romboutsia.This study suggested that PRTE exhibited a noteworthy therapeutic effect on UC via restoring intestinal barrier function and maintaining gut microbiota homeostasis,which develops a safe and efficient novel strategy for UC prophylaxis and therapy,and provides theoretical support for medical application of Pu’er raw tea in the future.展开更多
Non-alcoholic fatty liver disease(NAFLD)is a clinical syndrome associated with steatosis and excessive deposition in the liver,while safe,green and efficient curative therapy is still lacking.Current researches have s...Non-alcoholic fatty liver disease(NAFLD)is a clinical syndrome associated with steatosis and excessive deposition in the liver,while safe,green and efficient curative therapy is still lacking.Current researches have shown that plant polyphenols possess superior lipid-lowering and liver protective activities.Pu'er raw tea,a widely consumed tea with increasing fans,has been reported with various healthcare functions,such as blood lipids-lowering,weight loss,anti-microbes and anti-cancer.Therefore,in this study,polyphenols were extracted,purified and identified from Pu'er raw tea.The beneficial effects of Pu'er raw tea extract(PTE)on lipidoses in both LO2 cells and Caenorhabditis elegans were investigated.In vitro,PTE manifested beneficial effects in protecting cells from oxidative stress,alleviating lipid accumulation and expression of metabolic transcription factors such as Pparα,CD36,Plin2,Scd1,and activating the SIRT1-FOXO pathway to inhibit the expression of SREBP-1c and FAS.In vivo,PTE regulated the abnormal athletic ability of C.elegans and inhibited lipidosis in the whole body induced by oleic acid(OA).Therefore,PTE has exhibited superior bioactivity in alleviating lipid deposition both in vivo and in vitro,and could be regarded as a potential therapeutic strategy for NAFLD in the future.展开更多
基金supported by the program of China Poverty Allevia-tion through Education 2021,the National Natural Science Foundation of China(22206167)the Natural Science Foundation of Zhejiang Province(Z23C160017)the National Natural Science Foundation of China(32102103).
文摘Numerous investigations have uncovered the outstanding therapeutic effect of tea extract on ulcerative colitis(UC).However,the therapeutic potential on UC of Pu’er raw tea extract(PRTE)remains debated and need to be investigated.In our study,PRTE(100 and 300 mg/kg)was applied to the DSS-induced colitis mice via intra-gastric administration.Results have shown that PRTE treatment significantly ameliorated DSS-induced weight loss,loose stool,hematochezia,colon atrophy,splenomegaly and colon pathological injury.It could also inhibit UC by down-regulating pro-inflammatory cytokines(IL-1β,IL-6 and TNF-α)and increasing the expression of tight junction protein(zonula occludens-1,ZO-1)to reinforce intestinal barrier.Fecal microbiota transplantation has further confirmed that PRTE could maintain intestinal homeostasis and relieve UC by improve the abundance of beneficial bacteria such as Lactobacillus,Muribaculum,Lachnospiraceae and Rikenellaceae,and reduce the abun-dance of harmful bacteria such as Turicibacter and Romboutsia.This study suggested that PRTE exhibited a noteworthy therapeutic effect on UC via restoring intestinal barrier function and maintaining gut microbiota homeostasis,which develops a safe and efficient novel strategy for UC prophylaxis and therapy,and provides theoretical support for medical application of Pu’er raw tea in the future.
基金supported by the program of China Poverty Alleviation through Education 2021.
文摘Non-alcoholic fatty liver disease(NAFLD)is a clinical syndrome associated with steatosis and excessive deposition in the liver,while safe,green and efficient curative therapy is still lacking.Current researches have shown that plant polyphenols possess superior lipid-lowering and liver protective activities.Pu'er raw tea,a widely consumed tea with increasing fans,has been reported with various healthcare functions,such as blood lipids-lowering,weight loss,anti-microbes and anti-cancer.Therefore,in this study,polyphenols were extracted,purified and identified from Pu'er raw tea.The beneficial effects of Pu'er raw tea extract(PTE)on lipidoses in both LO2 cells and Caenorhabditis elegans were investigated.In vitro,PTE manifested beneficial effects in protecting cells from oxidative stress,alleviating lipid accumulation and expression of metabolic transcription factors such as Pparα,CD36,Plin2,Scd1,and activating the SIRT1-FOXO pathway to inhibit the expression of SREBP-1c and FAS.In vivo,PTE regulated the abnormal athletic ability of C.elegans and inhibited lipidosis in the whole body induced by oleic acid(OA).Therefore,PTE has exhibited superior bioactivity in alleviating lipid deposition both in vivo and in vitro,and could be regarded as a potential therapeutic strategy for NAFLD in the future.
