OBJECTIVE: Exploring the effect of Optimized New Shengmai powder(优化新生脉散方, ONSMP) on myocardial fibrosis in heart failure(HF) based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated pro...OBJECTIVE: Exploring the effect of Optimized New Shengmai powder(优化新生脉散方, ONSMP) on myocardial fibrosis in heart failure(HF) based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated protein kinase kinase(MEK)/extracellular regulated protein kinases(ERK) signaling pathway. METHODS: Randomized 70 Sprague-Dawley rats into sham(n = 10) and operation(n = 60) groups, then established the HF rat by ligating the left anterior descending branch of the coronary artery. We randomly divided the operation group rats into the model, ONSMP [including low(L), medium(M), and high(H) dose], and enalapril groups. After the 4-week drug intervention, echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight. Finally, we observed the degree of myocardial fibrosis by pathological sections, determined myocardium collagen(COL) Ⅰ and COL Ⅲ content by enzyme-linked immunosorbent assay, detected the m RNA levels of COL Ⅰ, COL Ⅲ, α-smooth muscle actin(α-SMA), and c-Fos proto-oncogene(c-Fos) by universal real-time, and detected the protein expression of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ETS-like-1 transcription factor(p-ELK1), p-c-Fos, α-SMA, COL Ⅰ, and COL Ⅲ by Western blot. RESULTS: ONSMP can effectively improve HF rat's cardiac function, decrease cardiac organ coefficient, COL volume fraction, and COL Ⅰ/Ⅲ content, down-regulate the m RNA of COL Ⅰ/Ⅲ, α-SMA and c-Fos, and the protein of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ELK1, c-Fos, COL Ⅰ/Ⅲ, and α-SMA. CONCLUSIONS: ONSMP can effectively reduce myocardial fibrosis in HF rats, and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.展开更多
In this work,hybrid material CNTs@MIL-101(Cr) was synthesized in 2 h using multi-walled carbon nanotubes(MWCNTs) as the crystal growth accelerator with hydrothermal method.The characteristic differences between the cr...In this work,hybrid material CNTs@MIL-101(Cr) was synthesized in 2 h using multi-walled carbon nanotubes(MWCNTs) as the crystal growth accelerator with hydrothermal method.The characteristic differences between the crystals of CNTs@MIL-101(Cr) and MIL-101 were investigated by N_2 adsorption–desorption isotherms,X-ray diffraction(XRD),scanning electron microscope(SEM) and thermogravimetric analyzer(TGA).The results showed that MWCNTs embedding in the hybrid material provide more mesoporous volumes than that of MIL-101.Moreover,the fast synthesized crystals of CNTs@MIL-101(Cr) still preserve the octahedral shape like MIL-101 and have a larger size ranging from 1.5 to 2.0 μm which were approximately three times larger than that of MIL-101.In the proposed mechanism,the roles of MWCNTs played in the crystallization were discussed where MWCNTs can be seen as coaxial cylindrical tubes composed of multi-layer graphenes and the place where nucleation and crystal growth processes occur at the tubes' out surface.Then,a crystal seeding layer bonding with the MWCNTs may be easily formed which accelerates the growth rate of MIL-101 crystals.Thus,larger crystals of CNTs@MIL-101(Cr) were formed due to the faster crystal growth rate of MIL-101.展开更多
基金Innovation Team Development Program of the Ministry of Education:Research on the Prevention and Treatment of Cardiovascular Diseases with Traditional Chinese Medicine (IRT-16R54)。
文摘OBJECTIVE: Exploring the effect of Optimized New Shengmai powder(优化新生脉散方, ONSMP) on myocardial fibrosis in heart failure(HF) based on rat sarcoma(RAS)/rapidly accelerated fibrosarcoma(RAF)/mitogen-activated protein kinase kinase(MEK)/extracellular regulated protein kinases(ERK) signaling pathway. METHODS: Randomized 70 Sprague-Dawley rats into sham(n = 10) and operation(n = 60) groups, then established the HF rat by ligating the left anterior descending branch of the coronary artery. We randomly divided the operation group rats into the model, ONSMP [including low(L), medium(M), and high(H) dose], and enalapril groups. After the 4-week drug intervention, echocardiography examines the cardiac function and calculates the ratios of the whole/left heart to the rat's body weight. Finally, we observed the degree of myocardial fibrosis by pathological sections, determined myocardium collagen(COL) Ⅰ and COL Ⅲ content by enzyme-linked immunosorbent assay, detected the m RNA levels of COL Ⅰ, COL Ⅲ, α-smooth muscle actin(α-SMA), and c-Fos proto-oncogene(c-Fos) by universal real-time, and detected the protein expression of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ETS-like-1 transcription factor(p-ELK1), p-c-Fos, α-SMA, COL Ⅰ, and COL Ⅲ by Western blot. RESULTS: ONSMP can effectively improve HF rat's cardiac function, decrease cardiac organ coefficient, COL volume fraction, and COL Ⅰ/Ⅲ content, down-regulate the m RNA of COL Ⅰ/Ⅲ, α-SMA and c-Fos, and the protein of p-RAS, p-RAF, p-MEK1/2, p-ERK1/2, p-ELK1, c-Fos, COL Ⅰ/Ⅲ, and α-SMA. CONCLUSIONS: ONSMP can effectively reduce myocardial fibrosis in HF rats, and the mechanism may be related to the inhibition of the RAS/RAF/MEK/ERK signaling pathway.
基金Supported by the National Natural Science Foundation of China(21006053)
文摘In this work,hybrid material CNTs@MIL-101(Cr) was synthesized in 2 h using multi-walled carbon nanotubes(MWCNTs) as the crystal growth accelerator with hydrothermal method.The characteristic differences between the crystals of CNTs@MIL-101(Cr) and MIL-101 were investigated by N_2 adsorption–desorption isotherms,X-ray diffraction(XRD),scanning electron microscope(SEM) and thermogravimetric analyzer(TGA).The results showed that MWCNTs embedding in the hybrid material provide more mesoporous volumes than that of MIL-101.Moreover,the fast synthesized crystals of CNTs@MIL-101(Cr) still preserve the octahedral shape like MIL-101 and have a larger size ranging from 1.5 to 2.0 μm which were approximately three times larger than that of MIL-101.In the proposed mechanism,the roles of MWCNTs played in the crystallization were discussed where MWCNTs can be seen as coaxial cylindrical tubes composed of multi-layer graphenes and the place where nucleation and crystal growth processes occur at the tubes' out surface.Then,a crystal seeding layer bonding with the MWCNTs may be easily formed which accelerates the growth rate of MIL-101 crystals.Thus,larger crystals of CNTs@MIL-101(Cr) were formed due to the faster crystal growth rate of MIL-101.
