Objective: To analyze the phytochemical constituents responsible for the plausible antioxidant effect of methanolic extract of the seed, pulp and peel of Baccaurea ramiflora Lour. Methods: Fresh seed, pulp, and peel o...Objective: To analyze the phytochemical constituents responsible for the plausible antioxidant effect of methanolic extract of the seed, pulp and peel of Baccaurea ramiflora Lour. Methods: Fresh seed, pulp, and peel of Baccaurea ramiflora fruits were extracted with methanol(MEBRse, MEBRpu, MEBRpe) and evaluated by phytochemical analysis for their content of innumerable metabolites(primary and secondary) viz. carbohydrates, alkaloids, glycosides, tannins, phenols, terpenoids, flavonoids, proteins, and fixed oils. The antioxidant efficacy was assessed through different assay methods viz. 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity, total antioxidant capacity(TAC) and reducing power capacity(RPC). Estimation of total phenolic content(TPC), and total flavonoid content(TFC) was also done to confirm the presence of these phytochemicals. Results: It was revealed from the phytochemical analysis of MEBRse that alkaloids, glycosides, carbohydrates, phenols, and flavonoids were present, while that of MEBRpu showed the existence of carbohydrates, proteins, alkaloids, glycosides, phenols, saponins, flavonoids, and fixed oils. Presence of carbohydrates, alkaloids, phenols, tannins, flavonoids, and terpenoids were found in the MEBRpe. A significant antioxidant activity was revealed by the MEBRpu [EC_(50):(27.612 ± 1.375) μg/mL], compared to MEBRpe, and MEBRse in DPPH assay. The ranking order for RPC was MEBRpu > MEBRpe > MEBRse respectively. The EC_(50) value of TAC of the MEBRpu, MEBRpe, and MEBRse were(25.107 ±0.744) μg/mL,(241.127 ± 7.463) μg/mL and(372.364 ± 11.030) μg/mL, respectively. Quantity of TPC and TFC were the highest in the MEBRpu(124.360 ± 2.078 mg GAE/g and 107.527 ±1.900 mg QRE/g extract) rather than MEBRpe and MEBRse extracts. Conclusions: This study suggests that MEBRpu has a significantly higher antioxidant property than MEBRpe and MEBRse. These extracts might be advantageous in prevention or decelerating the progress of different diseases related to oxidative-stress/damage. Moreover, detailed analysis of these extracts is required to identify the presence of promising compound(s) responsible for their antioxidant activity.展开更多
Among the numerous fruit species of Baccaurea ramiflora Lour.,‘LR’(white flesh)and‘BR’(pink flesh)are two kinds of local strains with high edibility.In order to study the metabolic causes of taste differences,we p...Among the numerous fruit species of Baccaurea ramiflora Lour.,‘LR’(white flesh)and‘BR’(pink flesh)are two kinds of local strains with high edibility.In order to study the metabolic causes of taste differences,we performed the non-targeted metabonomics analysis of‘LR’and‘BR’using LC-MS/MS.541 metabolites were totally identified,and 45 kinds of metabolites(carbohydrates,fatty acids,flavonoids and terpenoids,etc.)were different between the two strains.The results indicate L-sorbose,D-(+)-glucose,citric acid,L-phenylalanine and oleamide,α-eleostearic acid were the main primary metabolites.The significant difference existed in pathways of unsaturated fatty acids between the studied two strains by pathway enrichment analysis.The results demonstrate that the different in composition,as well as the abundance of primary and secondary metabolites may be the potential causes of taste differences,which provides a new insight into the possible metabolic factors setting off the changing taste of B.ramiflora.展开更多
[Objectives]The paper was to understand the species and harm of diseases and insect pests of Baccaurea ramiflora Lour.in Guangxi,and to provide basis for the prevention and control of diseases and insect pests.[Method...[Objectives]The paper was to understand the species and harm of diseases and insect pests of Baccaurea ramiflora Lour.in Guangxi,and to provide basis for the prevention and control of diseases and insect pests.[Methods]From 2018 to 2021,a systematic investigation on diseases and insect pests of B.ramiflora was conducted in Dongxing City,Fangchenggang City,Longzhou County,Pingxiang City,Jingxi City and Napo County in Guangxi.[Results]There were 22 species of diseases and insect pests that harmed B.ramiflora in Guangxi,7 of which were diseases and 15 were insect pests.Leaf blight(Fuasrium spp.)was the main disease at seedling stage,causing severe damage,and anthracnose(Colletotrichum gloeosporioides Penz.)caused moderate damage.Cyclosia papilionaris Drury and Chrysochus chinensis Baly had high frequency of occurrence,wide damage area and severe damage.Cyclosia panthono Stoll and Lcerya aegyptiaca Douglas caused moderate damage,while other insect pests caused light damage.[Conclusions]The study lays a foundation for the scientific prevention and control of B.ramiflora diseases and insect pests,and promotes the sustainable and rapid development of B.ramiflora industry in Guangxi.展开更多
The stem bark of </span><i><span style="font-family:Verdana;">Baccaurea ramiflora</span></i><span style="font-family:Verdana;"> was studied. Four aldehydes named...The stem bark of </span><i><span style="font-family:Verdana;">Baccaurea ramiflora</span></i><span style="font-family:Verdana;"> was studied. Four aldehydes named as 3</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">methoxy</span><span style="font-family:""> </span><span style="font-family:Verdana;">4</span><span style="font-family:""> </span><span style="font-family:Verdana;">hydroxy</span><span style="font-family:Verdana;">-</span><span style="font-family:Verdana;">cinnamaldehyde (coniferyl aldehyde);3,</span><span style="font-family:""> </span><span style="font-family:Verdana;">4,</span><span style="font-family:""> </span><span style="font-family:Verdana;">5</span><span style="font-family:""> </span><span style="font-family:Verdana;">trimethoxy</span><span style="font-family:""> </span><span style="font-family:Verdana;">cinnamaldehyde;3,</span><span style="font-family:""> </span><span style="font-family:Verdana;">4,</span><span style="font-family:""> </span><span style="font-family:Verdana;">5</span><span style="font-family:""> </span><span style="font-family:Verdana;">trimethoxy</span><span style="font-family:""> </span><span style="font-family:Verdana;">benzaldehyde and 3,4</span><span style="font-family:""> </span><span style="font-family:Verdana;">dimethoxy</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">benzaldehyde) (veratraldehyde) have been isolated and then identified by NMR spectroscopy. All of them are first time reported for this plant. Here </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> biological investigations include antioxidant and cytotoxicity study. Among all fractions, the chloroform soluble fraction exhibited strong free radical scavenging activity having IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value of 12.87 μg/ml compared to BHT (IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value 5.64 μg/ml). On the contrary, aqueous soluble fraction exhibited most toxicity towards brine shrimp compared with vincristine sulphate having LC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value of 1.44 and 0.9258 μg/ml respectively.展开更多
文摘Objective: To analyze the phytochemical constituents responsible for the plausible antioxidant effect of methanolic extract of the seed, pulp and peel of Baccaurea ramiflora Lour. Methods: Fresh seed, pulp, and peel of Baccaurea ramiflora fruits were extracted with methanol(MEBRse, MEBRpu, MEBRpe) and evaluated by phytochemical analysis for their content of innumerable metabolites(primary and secondary) viz. carbohydrates, alkaloids, glycosides, tannins, phenols, terpenoids, flavonoids, proteins, and fixed oils. The antioxidant efficacy was assessed through different assay methods viz. 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity, total antioxidant capacity(TAC) and reducing power capacity(RPC). Estimation of total phenolic content(TPC), and total flavonoid content(TFC) was also done to confirm the presence of these phytochemicals. Results: It was revealed from the phytochemical analysis of MEBRse that alkaloids, glycosides, carbohydrates, phenols, and flavonoids were present, while that of MEBRpu showed the existence of carbohydrates, proteins, alkaloids, glycosides, phenols, saponins, flavonoids, and fixed oils. Presence of carbohydrates, alkaloids, phenols, tannins, flavonoids, and terpenoids were found in the MEBRpe. A significant antioxidant activity was revealed by the MEBRpu [EC_(50):(27.612 ± 1.375) μg/mL], compared to MEBRpe, and MEBRse in DPPH assay. The ranking order for RPC was MEBRpu > MEBRpe > MEBRse respectively. The EC_(50) value of TAC of the MEBRpu, MEBRpe, and MEBRse were(25.107 ±0.744) μg/mL,(241.127 ± 7.463) μg/mL and(372.364 ± 11.030) μg/mL, respectively. Quantity of TPC and TFC were the highest in the MEBRpu(124.360 ± 2.078 mg GAE/g and 107.527 ±1.900 mg QRE/g extract) rather than MEBRpe and MEBRse extracts. Conclusions: This study suggests that MEBRpu has a significantly higher antioxidant property than MEBRpe and MEBRse. These extracts might be advantageous in prevention or decelerating the progress of different diseases related to oxidative-stress/damage. Moreover, detailed analysis of these extracts is required to identify the presence of promising compound(s) responsible for their antioxidant activity.
基金Forestry Science and Technology Innovation Project of Guangdong province(2018KJCX023)National Science Foundation for Young Scientists of China(31801742)+2 种基金Guangdong Key Laboratory for Functional Substances in Medicinal Edible Resources and Healthcare Products(2021B1212040015)Science and Technology Major Project of Guangxi(Guike AA17204045-4)Science and Technology Project of Chongzuo,Guangxi(Chongkegong 2019029).
文摘Among the numerous fruit species of Baccaurea ramiflora Lour.,‘LR’(white flesh)and‘BR’(pink flesh)are two kinds of local strains with high edibility.In order to study the metabolic causes of taste differences,we performed the non-targeted metabonomics analysis of‘LR’and‘BR’using LC-MS/MS.541 metabolites were totally identified,and 45 kinds of metabolites(carbohydrates,fatty acids,flavonoids and terpenoids,etc.)were different between the two strains.The results indicate L-sorbose,D-(+)-glucose,citric acid,L-phenylalanine and oleamide,α-eleostearic acid were the main primary metabolites.The significant difference existed in pathways of unsaturated fatty acids between the studied two strains by pathway enrichment analysis.The results demonstrate that the different in composition,as well as the abundance of primary and secondary metabolites may be the potential causes of taste differences,which provides a new insight into the possible metabolic factors setting off the changing taste of B.ramiflora.
基金Supported by Fundamental Research Project of Guangxi Academy of Agricultural Sciences(GNK 2020YM45)Stable Funding Team Project of Guangxi Academy of Agricultural Sciences(GK 2021YT168)。
文摘[Objectives]The paper was to understand the species and harm of diseases and insect pests of Baccaurea ramiflora Lour.in Guangxi,and to provide basis for the prevention and control of diseases and insect pests.[Methods]From 2018 to 2021,a systematic investigation on diseases and insect pests of B.ramiflora was conducted in Dongxing City,Fangchenggang City,Longzhou County,Pingxiang City,Jingxi City and Napo County in Guangxi.[Results]There were 22 species of diseases and insect pests that harmed B.ramiflora in Guangxi,7 of which were diseases and 15 were insect pests.Leaf blight(Fuasrium spp.)was the main disease at seedling stage,causing severe damage,and anthracnose(Colletotrichum gloeosporioides Penz.)caused moderate damage.Cyclosia papilionaris Drury and Chrysochus chinensis Baly had high frequency of occurrence,wide damage area and severe damage.Cyclosia panthono Stoll and Lcerya aegyptiaca Douglas caused moderate damage,while other insect pests caused light damage.[Conclusions]The study lays a foundation for the scientific prevention and control of B.ramiflora diseases and insect pests,and promotes the sustainable and rapid development of B.ramiflora industry in Guangxi.
文摘The stem bark of </span><i><span style="font-family:Verdana;">Baccaurea ramiflora</span></i><span style="font-family:Verdana;"> was studied. Four aldehydes named as 3</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">methoxy</span><span style="font-family:""> </span><span style="font-family:Verdana;">4</span><span style="font-family:""> </span><span style="font-family:Verdana;">hydroxy</span><span style="font-family:Verdana;">-</span><span style="font-family:Verdana;">cinnamaldehyde (coniferyl aldehyde);3,</span><span style="font-family:""> </span><span style="font-family:Verdana;">4,</span><span style="font-family:""> </span><span style="font-family:Verdana;">5</span><span style="font-family:""> </span><span style="font-family:Verdana;">trimethoxy</span><span style="font-family:""> </span><span style="font-family:Verdana;">cinnamaldehyde;3,</span><span style="font-family:""> </span><span style="font-family:Verdana;">4,</span><span style="font-family:""> </span><span style="font-family:Verdana;">5</span><span style="font-family:""> </span><span style="font-family:Verdana;">trimethoxy</span><span style="font-family:""> </span><span style="font-family:Verdana;">benzaldehyde and 3,4</span><span style="font-family:""> </span><span style="font-family:Verdana;">dimethoxy</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">benzaldehyde) (veratraldehyde) have been isolated and then identified by NMR spectroscopy. All of them are first time reported for this plant. Here </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> biological investigations include antioxidant and cytotoxicity study. Among all fractions, the chloroform soluble fraction exhibited strong free radical scavenging activity having IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value of 12.87 μg/ml compared to BHT (IC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value 5.64 μg/ml). On the contrary, aqueous soluble fraction exhibited most toxicity towards brine shrimp compared with vincristine sulphate having LC</span><sub><span style="font-family:Verdana;">50</span></sub><span style="font-family:Verdana;"> value of 1.44 and 0.9258 μg/ml respectively.
