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明恩溥的无本译写:Chinese Characteristics的版本流变与人物特质
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作者 苏蕊 亚晓晗 《外国语文研究》 2025年第2期55-71,共17页
Chinese Characteristics作为重要的域外文献,以他者视野译写了晚清时期中国社会面貌及中国人特征。本研究以无本译写为主体理据,首先通过版本流变考察明恩溥在译写语言上的特点,由此反观其译写意图及思想来源,并深入剖析其思维认知的改... Chinese Characteristics作为重要的域外文献,以他者视野译写了晚清时期中国社会面貌及中国人特征。本研究以无本译写为主体理据,首先通过版本流变考察明恩溥在译写语言上的特点,由此反观其译写意图及思想来源,并深入剖析其思维认知的改变;进而针对1894年修订版进行碎片式底本溯源。选取文本中的典型案例,分析其译写语言内部的中国人特质,以期挖掘明恩溥译写现象背后的社会文化因素。本研究对Chinese Characteristics的考释,不仅有助于了解西方人眼中的中国国民特质,还可以此为镜,揭示明恩溥他塑中国形象中的正与偏,为今后借力使力,塑造可信、可敬、可爱的中国形象提供借鉴和参考。 展开更多
关键词 无本译写 底本溯源 版本流变 国民特质 他者视野
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SiO_(2)'-CaO/(CaO+K_(2)O)(S'CK)Diagram as a Petrochemical Tool for Deciphering the Tectono-Magmatic Characterization of Granitoid Suites 被引量:1
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作者 SUN Mingming WANG Yang 《Acta Geologica Sinica(English Edition)》 2025年第2期598-610,共13页
The SiO_(2)'-CaO/(CaO+K_(2)O)(S'CK)diagram is an empirically derived major element-based equivalent to the modal IUGS alkali feldspar-quartz-plagioclase classification scheme for granitoids.It employs the cont... The SiO_(2)'-CaO/(CaO+K_(2)O)(S'CK)diagram is an empirically derived major element-based equivalent to the modal IUGS alkali feldspar-quartz-plagioclase classification scheme for granitoids.It employs the content of SiO_(2)and CaO/(CaO+K_(2)O)ratio to approximate the IUGS classification diagram and a normative-based Q'-ANOR plot.Four trends have been superimposed onto the SiO_(2)'-CaO/(CaO+K_(2)O)diagram based on published datasets from the Peninsular Ranges(calcic:C),Tuolumne(calc-alkalic:CA),Sherman(alkali-calcic:AC),and Bjerkreim-Sokndal(alkalic:A)batholiths,which were employed to constrain the positions of the C-CA,CA-AC and AC-A suite boundaries on the SiO_(2)versus(Na_(2)O+K_(2)O-CaO)(or modified alkali-lime index,MALI)granitic classification diagram.A merit of the SiO_(2)'-CaO/(CaO+K_(2)O)plot is identifying rock types comprising a suite and their relative abundances.The distinguished projections of five typical granitoid assemblages,which are summarized by Bonin et al.(2020),demonstrate the ability of SiO_(2)'-CaO/(CaO+K_(2)O)diagram to decipher their petrogenesis.The SiO_(2)'-CaO/(CaO+K_(2)O)plots for the plutonic suites of'known'tectonic settings can reveal their evolution paths and the lithological statistics.Accordingly,it is suggested that the SiO_(2)'-CaO/(CaO+K_(2)O)plot can distinguish the tectonic environments of plutonic suits by comparing the plutonic suites or batholiths of'unknown'tectonic context to the published datasets from granitoid suites formed within'known'tectonic settings.The modified SiO_(2)'-CaO/(CaO+K_(2)O)diagram links the bulk chemical composition of granitoid suites to the likely source,magmatic evolution,and tectonic setting;thus,it may be a useful tectono-magmatic classification scheme for granitoid suites. 展开更多
关键词 classification of granitoid major-element chemistry SiO_(2)'–CaO/(CaO+K_(2)O)diagram tectono-magmatic character
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A Convolutional Neural Network Based Optical Character Recognition for Purely Handwritten Characters and Digits
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作者 Syed Atir Raza Muhammad Shoaib Farooq +3 位作者 Uzma Farooq Hanen Karamti Tahir Khurshaid Imran Ashraf 《Computers, Materials & Continua》 2025年第8期3149-3173,共25页
Urdu,a prominent subcontinental language,serves as a versatile means of communication.However,its handwritten expressions present challenges for optical character recognition(OCR).While various OCR techniques have bee... Urdu,a prominent subcontinental language,serves as a versatile means of communication.However,its handwritten expressions present challenges for optical character recognition(OCR).While various OCR techniques have been proposed,most of them focus on recognizing printed Urdu characters and digits.To the best of our knowledge,very little research has focused solely on Urdu pure handwriting recognition,and the results of such proposed methods are often inadequate.In this study,we introduce a novel approach to recognizing Urdu pure handwritten digits and characters using Convolutional Neural Networks(CNN).Our proposed method utilizes convolutional layers to extract important features from input images and classifies them using fully connected layers,enabling efficient and accurate detection of Urdu handwritten digits and characters.We implemented the proposed technique on a large publicly available dataset of Urdu handwritten digits and characters.The findings demonstrate that the CNN model achieves an accuracy of 98.30%and an F1 score of 88.6%,indicating its effectiveness in detecting and classifyingUrdu handwritten digits and characters.These results have far-reaching implications for various applications,including document analysis,text recognition,and language understanding,which have previously been unexplored in the context of Urdu handwriting data.This work lays a solid foundation for future research and development in Urdu language detection and processing,opening up new opportunities for advancement in this field. 展开更多
关键词 Image processing natural language processing handwritten Urdu characters optical character recognition deep learning feature extraction CLASSIFICATION
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Analysis of the Complexity and Symbolic Significance of Lensky’s Character in Eugene Onegin
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作者 Shiwei Liang 《Journal of Contemporary Educational Research》 2025年第3期67-72,共6页
The character of Lensky in Eugene Onegin is one of the most discussed figures in the novel.He is the friend of Eugene Onegin and represents a contradictory personality.In the novel,Lensky displays a complex set of cha... The character of Lensky in Eugene Onegin is one of the most discussed figures in the novel.He is the friend of Eugene Onegin and represents a contradictory personality.In the novel,Lensky displays a complex set of characteristics,appearing both elegant and noble on the outside,while concealing a deep inner loneliness and conflict.His attitude toward love and his dissatisfaction with society make him a dramatic and profound character in the story.By analyzing the character of Lensky,we can explore his role in the novel and his relationships with other characters.Lensky’s presence not only enriches the plot but also presents a figure filled with inner contradictions and emotional struggles.His friendship and rivalry with Eugene Onegin,as well as his admiration and helplessness in regard to Olga,showcase his complex inner world and reflections on life.Furthermore,the character of Lensky carries a certain symbolic significance.He can be seen as a metaphor for Russian society and culture at the time.His loneliness and inner conflict symbolize the limitations of Russian society and culture,while also reflecting Pushkin’s idealized pursuit of love and friendship,as well as his critical view of reality.In conclusion,through a deep analysis of Lensky’s character,we can better understand the portrayal of characters and the development of the plot in Eugene Onegin.It also provides readers with a perspective on Pushkin’s thoughts and observations on human nature,society,and culture. 展开更多
关键词 character ANALYSIS PERSONALITY Symbolic significance
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Rediscovery and Revision of the Diagnostic Characters of Opisthotropis daovantieni Orlov, Darevsky, and Murphy, 1998(Squamata:Natricidae)from Southern Vietnam, with Notes on its Hemipenial Morphology and Defensive Behavior
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作者 Zongyuan GAO Yong ZHANG +4 位作者 Vu Dang Hoang NGUYEN Ke JIANG Tao Thien NGUYEN Jiatang LI Jinlong REN 《Asian Herpetological Research》 2025年第1期49-65,共17页
The Tien's Mountain Stream Snake,Opisthotropis daovantieni Orlov, Darevsky, and Murphy, 1998, has been represented solely by its type series, with no additional specimens reported in the past two decades. As a res... The Tien's Mountain Stream Snake,Opisthotropis daovantieni Orlov, Darevsky, and Murphy, 1998, has been represented solely by its type series, with no additional specimens reported in the past two decades. As a result, limited data exist and O. daovantieni remains one of the least studied members of its genus. Based on a re-examination of the type series, analysis of newly collected topotypic specimens, and a review of museum collections, this study provides an updated and comprehensive morphological characterization of O. daovantieni including detailed descriptions of hemipenial morphology, revised diagnostic characters,phylogenetic positioning, and ecological insights.Based on morphological comparisons with congeners, we also define the informal Opisthotropis spenceri group to facilitate future taxonomic work. In addition, this study documents a previously unreported defensive behavior involving tail-poking,observed in the field and thus far unique within the genus Opisthotropis. 展开更多
关键词 diagnostic characters HEMIPENIS Opisthotropis spenceri group sulcus spermaticus tail-poking behavior
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Multiparameter generalized universal characters and multiparameter generalized B-type universal characters
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作者 Jingfan Wang Zhaowen Yan 《Chinese Physics B》 2025年第1期215-226,共12页
We construct the quantum fields presentation of the generalized universal character and the generalized B-type universal character,and by acting the quantum fields presentations to the constant 1,the generating functi... We construct the quantum fields presentation of the generalized universal character and the generalized B-type universal character,and by acting the quantum fields presentations to the constant 1,the generating functions are derived.Furthermore,we introduce two integrable systems known as the generalized UC(GUC)hierarchy and the generalized Btype UC(GBUC)hierarchy satisfied by the generalized universal character and the generalized B-type universal character,respectively.Based on infinite sequences of complex numbers,we further establish the multiparameter generalized universal character and the multiparameter generalized B-type universal character,which have been proved to be solutions of the GUC hierarchy and the GBUC hierarchy,respectively. 展开更多
关键词 symmetric functions quantum fields generating functions multiparameter generalized universal characters
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Symbolic Design Strategies for Film and Television Character Modeling in Cross-Cultural Contexts
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作者 Jianwei Yang 《Journal of Contemporary Educational Research》 2025年第10期369-375,共7页
Against the backdrop of the deep integration of the global film and television industry,character design has long transcended the mere pursuit of aesthetics and has become a key symbolic system that carries cultural i... Against the backdrop of the deep integration of the global film and television industry,character design has long transcended the mere pursuit of aesthetics and has become a key symbolic system that carries cultural information,builds identity recognition,and drives narrative.This article,by integrating semiotic theory and cultural dimension analysis,deeply dissects the core functions and common predicaments(stereotypes,cultural misinterpretation)of formative symbols in cross-cultural communication and systematically proposed four core design strategies:“extraction and translation of cultural symbols,”“visual mapping of cultural dimensions,”“symbolic support of narrative functions,”and“cultural decoding presuppositions for target audiences.”This article holds that successful cross-cultural modeling design should be committed to creative transformation on the basis of respecting the authenticity of culture,constructing a visual symbol system that combines cultural depth and universal appeal,and ultimately serving global narratives and in-depth cultural dialogues. 展开更多
关键词 Cross-cultural communication Film and television character modeling Cultural dimension
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An Analysis of the Necessity of Establishing a Case Library for International Chinese Character Teaching
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作者 ZHOU Yin 《Journal of Literature and Art Studies》 2025年第9期719-724,共6页
As an important method of practical teaching,case-based teaching has become increasingly prominent in international Chinese language education.However,the development of case-based teaching for Chinese characters rema... As an important method of practical teaching,case-based teaching has become increasingly prominent in international Chinese language education.However,the development of case-based teaching for Chinese characters remains insufficient,with limited research outcomes,making it difficult to effectively support the teaching and research of Chinese character instruction.The establishment of a case library for international Chinese character teaching can provide a wealth of teaching cases,meeting the developmental needs of international Chinese language education in the new era. 展开更多
关键词 international Chinese language education Chinese character teaching case library
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Deep Learning-Based Natural Language Processing Model and Optical Character Recognition for Detection of Online Grooming on Social Networking Services
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作者 Sangmin Kim Byeongcheon Lee +2 位作者 Muazzam Maqsood Jihoon Moon Seungmin Rho 《Computer Modeling in Engineering & Sciences》 2025年第5期2079-2108,共30页
The increased accessibility of social networking services(SNSs)has facilitated communication and information sharing among users.However,it has also heightened concerns about digital safety,particularly for children a... The increased accessibility of social networking services(SNSs)has facilitated communication and information sharing among users.However,it has also heightened concerns about digital safety,particularly for children and adolescents who are increasingly exposed to online grooming crimes.Early and accurate identification of grooming conversations is crucial in preventing long-term harm to victims.However,research on grooming detection in South Korea remains limited,as existing models trained primarily on English text and fail to reflect the unique linguistic features of SNS conversations,leading to inaccurate classifications.To address these issues,this study proposes a novel framework that integrates optical character recognition(OCR)technology with KcELECTRA,a deep learning-based natural language processing(NLP)model that shows excellent performance in processing the colloquial Korean language.In the proposed framework,the KcELECTRA model is fine-tuned by an extensive dataset,including Korean social media conversations,Korean ethical verification data from AI-Hub,and Korean hate speech data from Hug-gingFace,to enable more accurate classification of text extracted from social media conversation images.Experimental results show that the proposed framework achieves an accuracy of 0.953,outperforming existing transformer-based models.Furthermore,OCR technology shows high accuracy in extracting text from images,demonstrating that the proposed framework is effective for online grooming detection.The proposed framework is expected to contribute to the more accurate detection of grooming text and the prevention of grooming-related crimes. 展开更多
关键词 Online grooming KcELECTRA natural language processing optical character recognition social networking service text classification
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Nonreferential Uses of “I” and Its Perspectival Character
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作者 Minyao Huang 《逻辑学研究》 2025年第3期115-127,共13页
The English“T” is widely held as a well-behaved Kaplanian indexical that has a directly-referential content and a character which imples immunity to self misidentification.In this paper I present uses of“T”outside... The English“T” is widely held as a well-behaved Kaplanian indexical that has a directly-referential content and a character which imples immunity to self misidentification.In this paper I present uses of“T”outside attitudal contexts that are not directly referential yet exhibit immunity to self misidentification.They include uses of“I”for simulation and for counterfactual self portrait.I argue that they(i)challenge the non-shiftability and the rigidity arguments for the direct reference view,and(1)require a revision of the character of“T”to reflect the sensitivity of its content to the perspective from which the speaker identifies herself. 展开更多
关键词 Kaplanian indexical simulation counterfactual self portraiti perspectival character self misidentification counterfactual self portrait nonreferential uses
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Robust Multi-Label Cartoon Character Classification on the Novel Kral Sakir Dataset Using Deep Learning Techniques
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作者 Candan Tumer Erdal Guvenoglu Volkan Tunali 《Computers, Materials & Continua》 2025年第12期5135-5158,共24页
Automated cartoon character recognition is crucial for applications in content indexing,filtering,and copyright protection,yet it faces a significant challenge in animated media due to high intra-class visual variabil... Automated cartoon character recognition is crucial for applications in content indexing,filtering,and copyright protection,yet it faces a significant challenge in animated media due to high intra-class visual variability,where characters frequently alter their appearance.To address this problem,we introduce the novel Kral Sakir dataset,a public benchmark of 16,725 images specifically curated for the task of multi-label cartoon character classification under these varied conditions.This paper conducts a comprehensive benchmark study,evaluating the performance of state-of-the-art pretrained Convolutional Neural Networks(CNNs),including DenseNet,ResNet,and VGG,against a custom baseline model trained from scratch.Our experiments,evaluated using metrics of F1-Score,accuracy,and Area Under the ROC Curve(AUC),demonstrate that fine-tuning pretrained models is a highly effective strategy.The best-performing model,DenseNet121,achieved an F1-Score of 0.9890 and an accuracy of 0.9898,significantly outperforming our baseline CNN(F1-Score of 0.9545).The findings validate the power of transfer learning for this domain and establish a strong performance benchmark.The introduced dataset provides a valuable resource for future research into developing robust and accurate character recognition systems. 展开更多
关键词 Cartoon character recognition multi-label classification deep learning transfer learning predictive modelling artificial intelligence-enhanced(AI-Enhanced)systems Kral Sakir dataset
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Genetic and Correlation Analysis of Grain Characters in Three-line Hybrid Rice 被引量:11
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作者 邹小云 邓辉明 +6 位作者 傅军如 彭小松 朱昌兰 贺晓鹏 陈小荣 贺浩华 刘宜柏 《Agricultural Science & Technology》 CAS 2009年第2期77-81,共5页
Abst[Objective] This study was to understand the genetic dynamics of three-line hybrid rice, and explore the respective effect of sterile line and restoring line on grain characters of hybrid rica. [Method] Four three... Abst[Objective] This study was to understand the genetic dynamics of three-line hybrid rice, and explore the respective effect of sterile line and restoring line on grain characters of hybrid rica. [Method] Four three-line stedle lines and 27 restoring lines(cultivars) commonly culti- vated in Central China region were regarded as expadmental materials to conduct 4 x27NCII cross design, and the grain characters of three-line hybrid rico were analyzed at genetic and correlation levels. [ Result] Four characters of grain length, grain width, 1 000-grain weight and length- width ratio play the leading role in additive gene effect; these four characters were simultaneously influenced by male parent and female parent, but the effect from male parent was relatively larger. The grain length, grain width, 1 000-grain weight and length-width ratio all have high brood hedtabUities( respectively 99.65%, 98.31%, 95.27% and 98.81% ). Correlation analysis showed that grain length was positively correlated with 1 000-grain weight and length-width ratio at extremely significant level; 1 000-grain weight was positively correlated with grain length and length- width ratio at extremely significant level, and was insignificantly correlated with grain width; grain width was negatively correlated with grain length and length-width ratio at extremely significant level. Path analysis showed that the direct path coefficients of grain length, grain width and 1 0(30- grain weight to length-width ratio were 0.624 6, -0.555 9 and -0.015 8, respectively. [ Conclusion] This study systematically analyzed the effects of stedle line and restoring line on grain characters of hybrid rice, which provided theoretical basis for breeding high quality and yield hy- brid dce. 展开更多
关键词 Three-line hybrid dce Grain characters INHERITANCE . Correlation
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Study on the Change Character of Double Rice Cold Damage in Hunan and Hubei Province during the Past 50 Years 被引量:2
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作者 帅细强 蔡荣辉 +3 位作者 刘敏 万素琴 谢佰承 刘志雄 《Meteorological and Environmental Research》 CAS 2010年第3期17-20,41,共5页
Based on the meteorological data of representative meteorological stations in Hunan and Hubei from 1961 to 2008 years,the change character of double rice cold damage under the background of climate change were analyze... Based on the meteorological data of representative meteorological stations in Hunan and Hubei from 1961 to 2008 years,the change character of double rice cold damage under the background of climate change were analyzed adopting 30 years glide accumulation method.The analysis showed that the 30 years sliding cumulative days of mild low-temperature during spring sowing in Hunan and Hubei were decreased in the recent 50 years,as well as the 30 years sliding cumulative days of moderate low-temperature in central south Hubei,northern and western Hunan.However,there was an increase in southern Hunan,so was the 30 years sliding cumulative days of severe low-temperature.The 30 years sliding cumulative days of mild low-temperature in May reduced in Hunan and Hubei.Instead,that of moderate low-temperature in May in central south Hubei was on the rise,and it was opposite in northern and southern Hunan,but there was no obvious change in western Hunan.The 30 years sliding cumulative days of severe low-temperature showed a decreasing tendency in May in central south Hubei,northern and western Hunan,and southern Hunan showed a contrary tendency.The 30 years sliding cumulative days of mild cold dew wind in Hunan and Hubei were decreased,that of moderate cold dew wind in central south Hubei,northern and western Hunan had the same tendency,but there was a contrary tendency in southern Hunan.The 30 years sliding cumulative days of severe cold dew wind was decreased in central south Hubei and increased in western Hunan,without cold dew wind in northern and southern Hunan. 展开更多
关键词 Cold damage Change character Hubei and Hunan Province Double rice China
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Main Agronomic Characters and Grain Quality of Rice Blast Resistance Gene Pi-d2 Transgenic Rice 被引量:6
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作者 陈德西 曲广林 +2 位作者 黄文娟 李婷 李仕贵 《Agricultural Science & Technology》 CAS 2011年第10期1457-1460,共4页
[Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice wer... [Objective] The aim of this study was to provide metabolic evidence for the analysis of the ecological and safety assessment of Pi-d2-transgenic rice.[Method] The main agronomic characters of Pi-d2-transgenic rice were observed in field experiment and the grain chemical characters and amino acid content were measured.[Results] Introduction of foreign gene Pi-d2 resulted in stably hereditable variation in agronomic characteristics in the descents.Most of the transgenic lines grew normally and orderly.Compared with the control(wild type plants),about half of transgenic plants showed an increased or reduced plant height.There was no observable difference between transgenic plants and controls in tiller number,length of panicle,panicles per plant,seed-setting rate and 1 000-grain weight.Total amino acid content in transgenic rice was reduced,while the starch content,GC and GT were not altered in comparison with the control.[Conclusion] Introduction of foreign gene Pi-d2 has remarkable influence on plant height,while little on grain chemical characters. 展开更多
关键词 TRANSGENE Pi-d2 gene Rice Agronomic characters Amino acid
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Correlation Analysis of Characters of Indica Hybrid Rice with High Content of Amylose 被引量:4
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作者 杨扬 况浩池 +4 位作者 曾正明 罗俊涛 陈光珍 何兴材 付均 《Agricultural Science & Technology》 CAS 2013年第3期475-477,共3页
[Objective] The aim was to research and analyze characters and related correlation of indica hybrid rice containing high amylose. [Method] In the research, major characters of 16 species of groups of {ndica hybrid ric... [Objective] The aim was to research and analyze characters and related correlation of indica hybrid rice containing high amylose. [Method] In the research, major characters of 16 species of groups of {ndica hybrid rice and the correlation were explored in rice belts in Sichuan in 2011. [Result] The variation of chalkiness degree was the highest at 26.8%, followed by rate of head rice at 22.89%; varia- tions of head rice rate and chalkiness degree were lower at 0.72% and 2.61%. In addition, rate of unpolished rice was of significantly negative correlation with chalki- ness degree; rate of chalky rice was of extremely positive correlation with chalkiness degree and gel consistency; chalkiness degree was of significantly positive correla- tion with gel consistency; gel consistency was of significantly negative correlation with protein content; amylose content was of insignificant correlation with gel consis- tency (r=0.166 9) of the 16 hybrid groups. These indicated that gel consistency would be still high as the content of amylose grows (AC≥25%). For example, when the content of anylose in Chuanxiang 29A/Luhui 37, Kangfeng AJ6684, and Lefeng A/SR536 exceeded 27%, the gel consistency was over 80 mm in the research. [Conclusion] The research provides references for improvement of amylose content in breeding hybrid rice. 展开更多
关键词 Higher amylose content Indica hybrid rice characterS Correlaiton analysis
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Effects of Plastic Film Mulching on Physical Characters of Soil and Yield and Yield Components of Sweet Potato 被引量:6
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作者 张超凡 黄艳岚 +2 位作者 周虹 张亚 张道微 《Agricultural Science & Technology》 CAS 2015年第11期2379-2385,2393,共8页
Field experiments were carried out to study the effects of plastic film mulching on soil physical characters, including soil temperature, soil moisture content and soil bulk density, and yield and yield components of ... Field experiments were carried out to study the effects of plastic film mulching on soil physical characters, including soil temperature, soil moisture content and soil bulk density, and yield and yield components of sweet potato. The results showed that plastic filming mulching increased soil temperature. Considering the soil temperature-increasing effect, the treatments ranked as black plastic film treatment 〉 white plastic film treatment 〉 control. However, with the deepening of soil layer, the warming effect of plastic film mulching was weakened. Black or white plastic film mulching was conducive to low T/R value, especially in the early growth stage of sweet potato. Plastic film mulching significantly improved the storage root yield of sweet potato. In terms of yield-improving effect, the treatments ranked as black plastic film treatment 〉 white plastic film treatment 〉 control. The storage root num- ber per plant showed a downward trend, but the weight of single storage root was increased. 展开更多
关键词 Plastic film mulching Sweet potato Soil physical characters YIELD Growth and development
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Variation and Cluster Analysis on Leaf Characters from Different Provenance Sources of Polygonum multiflorum Thunb 被引量:2
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作者 韦艳梅 王凌晖 +2 位作者 曹福亮 韦山青 梁耀丹 《Agricultural Science & Technology》 CAS 2010年第6期94-98,共5页
[Objective] The aim was to study the variation of leaf characters from different provenance sources of Polygonum multiflorum Thunb,as well as to carry out cluster analysis on P.multiflorum from different provenance so... [Objective] The aim was to study the variation of leaf characters from different provenance sources of Polygonum multiflorum Thunb,as well as to carry out cluster analysis on P.multiflorum from different provenance sources to provide basis for the classification,identification,breeding and improved variety selection of P.multiflorum.[Method] Leaf shape characters of 31 copies of germplasm resources in the major distribution region of the whole country were determined,and the genetic variation of P.multiflorum leaves from different producing areas was analyzed.[Result] The leaf characters of single plant of the same experimental provenance source of P.multiflorum were relatively stable,the variation was mainly found on the single leaf area,1/2 leaf width,leaf width and other indicators;the variation of each leaf character among different provenance sources was obvious,and the variation was mainly found on the single leaf weight,leaf area,1/2 leaf width,leaf length and other indicators.The correlation analysis of each leaf character in P.multiflorum suggested that the single leaf area and single leaf weight showed extremely significant positive correlation with leaf length,1/2 leaf width,leaf width,leaf thickness and leaf stem length,while the single leaf area and single leaf weight showed significant negative correlation with WWR(leaf width/1/2 leaf width)and LWR(leaf length/1/2 leaf length),in addition,several macroscopic leaf characters such as leaf length,1/2 leaf width,leaf width,leaf stem length showed extremely positive correlation.The main component analysis result suggested that the contribution rate of accumulation variance of the front three main components was up to 97.4%,which could better reflect the comprehensive performance of leaf characters of different provenance sources of P.multiflorum.The cluster analysis showed that the experimental 31 copies of P.multiflorum provenance sources should be divided into three classes,the first class was distributed in the Middle,Western of Guizhou,northwestern of Guangxi and western areas with higher altitude;the second class was distributed in Hunan,Hubei,Sichuan,Guangdong and the most area of Guangxi;the third class was distributed in Anhui,Jiangsu and Henan and Shandong.[Conclusion] Cluster analysis of leaf characters indicated that the kinds of provenance sources which the geographical position was closer could be got together.The study had provided a certain basis for the classification of P.multiflorum. 展开更多
关键词 Polygonum multiflorum Thunb Leaf characters VARIATION Cluster analysis
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Physicochemical Characters of rPoIFN-α and Its Antiviral Activity in vitro 被引量:3
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作者 汤仁树 赵俊 王明丽 《Agricultural Science & Technology》 CAS 2010年第1期65-68,共4页
[Objective]The research aimed to test and identify the physicochemical characters and antiviral activity in vitro of semi-finished product of the recombinant porcine rPoIFN-α. [Method]HEp-2/VSV system was used to tes... [Objective]The research aimed to test and identify the physicochemical characters and antiviral activity in vitro of semi-finished product of the recombinant porcine rPoIFN-α. [Method]HEp-2/VSV system was used to test the antiviral activity of three batches of rPoIFN-α. Using recombinant human IFN-α as reference,the titer of interferon was measured. The semi-finished product of rPoIFN-α with the known titer were treated with 0.25% trypsin,HCl and mouse anti-porcine IFN-α monoclonal antibody. And the anti-viral activity of each batch of rPoIFN-α was detected. The physicochemical characters of rPoIFN-α were evaluated. The inhibition of induced cytopathic effect of rPoIFN-α on PPV (Porcine parvovirus) and PRV (Porcine pseudorabies) on swine kidney cell (PK-15) was determined. And the antiviral activity of rPoIFN-α in vitro was observed. [Result]The titers of semi-finished products of rPoIFN-α titrated by HEp-2/VSV system could reach 1.5×105 IU/ml,with the specific activity of 1.1×106 IU/mg. The residual rate of the tier of rPoIFN-α treated by 0.25% trypsin at 37 ℃ for 1 h was less than 1%. And that treated with HCl (pH of 2.0) for 72 h was up to 95%. And that treated at 56 ℃ for 30 min and that treated by mouse anti-porcine IFN-α monoclonal antibody at 37 ℃ for 1 h were higher than 47% and about 1% respectively. The antiviral test in vitro showed that 50 and 500 IU/ml rPoIFN-α could inhibit the induced cytopathic effect of PRV and PPV on PK-15 cell lines. [Conclusion]rPoIFN-α had the basic physicochemical characters of IFN-α. And it could inhibit the induced cytopathic effect of PRV and PPV on PK-15 cell lines,but there was dosage difference. 展开更多
关键词 Porcine interferon α Physicochemical character Antiviral activity
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