Quantitative phase microscopy(QPM)enables label-free imaging and precise characterization of transparent specimens by measuring phase delay.However,optical aberrations induce wavefront distortions that degrade phase r...Quantitative phase microscopy(QPM)enables label-free imaging and precise characterization of transparent specimens by measuring phase delay.However,optical aberrations induce wavefront distortions that degrade phase reconstruction accuracy,resolution,and contrast.Existing strategies require diverse measurements or iterative optimization,limiting flexibility for real-time applications.We propose an adaptive aberration-corrected QPM system enabled by a physics-informed cycle-consistent network(PICNet)without prior calibration.By incorporating a learnable physical forward model to approximate the practical image formation and enforcing cycle consistency between object and measurement domains,PICNet can reconstruct the object phase from a single-shot measurement while simultaneously inferring complex aberrations that are difficult to characterize explicitly.Our approach achieves a 60%improvement in structural similarity compared with uncorrected results.Experiments demonstrate that PICNet enables rapid and highfidelity phase retrieval across diverse biological samples with enhanced robustness to aberrations.This physically reliable and self-calibrating framework establishes a general paradigm for solving inverse problems across various computational imaging modalities.展开更多
Since the invention of Zernike phase contrast method in 1930,it has been widely used in optical microscopy and more recently in X-ray microscopy.Considering the image contrast is a mixture of absorption and phase info...Since the invention of Zernike phase contrast method in 1930,it has been widely used in optical microscopy and more recently in X-ray microscopy.Considering the image contrast is a mixture of absorption and phase information,we recently have proposed and demonstrated a method for quantitative phase retrieval in Zernike phase contrast X-ray microscopy.In this contribution,we analyze the performance of this method at different photon energies.Intensity images of PMMA samples are simulated at 2.5 keV and 6.2 keV,respectively,and phase retrieval is performed using the proposed method.The results demonstrate that the proposed phase retrieval method is applicable over a wide energy range.For weakly absorbing features,the optimal photon energy is 2.5 keV,from the point of view of image contrast and accuracy of phase retrieval.On the other hand,in the case of strong absorption objects,a higher photon energy is preferred to reduce the error of phase retrieval.These results can be used as guidelines to perform quantitative phase retrieval in Zernike phase contrast X-ray microscopy with the proposed method.展开更多
A new optical microscopy technique,termed high spatial and temporal resolution synthetic aperture phase microscopy(HISTR-SAPM),is proposed to improve the lateral resolution of wide-field coherent imaging.Under plane w...A new optical microscopy technique,termed high spatial and temporal resolution synthetic aperture phase microscopy(HISTR-SAPM),is proposed to improve the lateral resolution of wide-field coherent imaging.Under plane wave illumination,the resolution is increased by twofold to around 260 nm,while achieving millisecond-level temporal resolution.In HISTR-SAPM,digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability.An off-axis interferometer is used to measure the sample scattered complex fields,which are then processed to reconstruct high-resolution phase images.Using HISTR-SAPM,we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap(i.e.,a full pitch of 330 nm).As the reconstruction averages out laser speckle noise while maintaining high temporal resolution,HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells,such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells.We envision that HISTR-SAPM will broadly benefit research in material science and biology.展开更多
The transport of intensity equation(TIE)is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardw...The transport of intensity equation(TIE)is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardware.However,existing TIE methods encounter difficulties in decoupling the independent contributions of phase and aberrations to the measurements in the case of unknown pupil function.Additionally,spatially nonuniform and temporally varied aberrations dramatically degrade the imaging performance for long-term research.Hence,it remains a critical challenge to realize the high-throughput quantitative phase imaging(QPI)with aberration correction under partially coherent illumination.To address these issues,we propose a novel method for highthroughput microscopy with annular illumination,termed as transport-of-intensity QPI with aberration correction(TI-AC).By combining aberration correction and pixel super-resolution technique,TI-AC is made compatible with large pixel-size sensors to enable a broader field of view.Furthermore,it surpasses the theoretical Nyquist-Shannon sampling resolution limit,resulting in an improvement of more than two times.Experimental results demonstrate that the half-width imaging resolution can be improved to~345 nm across a 10×field of view of 1.77 mm^(2)(0.4 NA).Given its high-throughput capability for QPI,TI-AC is expected to be adopted in biomedical fields,such as drug discovery and cancer diagnostics.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.62235009,62305183,and 624B2080).
文摘Quantitative phase microscopy(QPM)enables label-free imaging and precise characterization of transparent specimens by measuring phase delay.However,optical aberrations induce wavefront distortions that degrade phase reconstruction accuracy,resolution,and contrast.Existing strategies require diverse measurements or iterative optimization,limiting flexibility for real-time applications.We propose an adaptive aberration-corrected QPM system enabled by a physics-informed cycle-consistent network(PICNet)without prior calibration.By incorporating a learnable physical forward model to approximate the practical image formation and enforcing cycle consistency between object and measurement domains,PICNet can reconstruct the object phase from a single-shot measurement while simultaneously inferring complex aberrations that are difficult to characterize explicitly.Our approach achieves a 60%improvement in structural similarity compared with uncorrected results.Experiments demonstrate that PICNet enables rapid and highfidelity phase retrieval across diverse biological samples with enhanced robustness to aberrations.This physically reliable and self-calibrating framework establishes a general paradigm for solving inverse problems across various computational imaging modalities.
基金Supported by the State Key Project for Fundamental Research(2012CB825801)National Natural Science Foundation of China(11475170,11205157 and 11179004)Anhui Provincial Natural Science Foundation(1508085MA20)
文摘Since the invention of Zernike phase contrast method in 1930,it has been widely used in optical microscopy and more recently in X-ray microscopy.Considering the image contrast is a mixture of absorption and phase information,we recently have proposed and demonstrated a method for quantitative phase retrieval in Zernike phase contrast X-ray microscopy.In this contribution,we analyze the performance of this method at different photon energies.Intensity images of PMMA samples are simulated at 2.5 keV and 6.2 keV,respectively,and phase retrieval is performed using the proposed method.The results demonstrate that the proposed phase retrieval method is applicable over a wide energy range.For weakly absorbing features,the optimal photon energy is 2.5 keV,from the point of view of image contrast and accuracy of phase retrieval.On the other hand,in the case of strong absorption objects,a higher photon energy is preferred to reduce the error of phase retrieval.These results can be used as guidelines to perform quantitative phase retrieval in Zernike phase contrast X-ray microscopy with the proposed method.
基金We acknowledge financial support from Hong Kong Innovation and Technology Fund(Nos.ITS/394/17 and ITS/098/18FP)Shun Hing Institute of Advanced Engineering(No.BME-p3-18)Croucher Innovation Awards 2019,and the U.S.National Institutes of Health(No.5P41EB015871-33).
文摘A new optical microscopy technique,termed high spatial and temporal resolution synthetic aperture phase microscopy(HISTR-SAPM),is proposed to improve the lateral resolution of wide-field coherent imaging.Under plane wave illumination,the resolution is increased by twofold to around 260 nm,while achieving millisecond-level temporal resolution.In HISTR-SAPM,digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability.An off-axis interferometer is used to measure the sample scattered complex fields,which are then processed to reconstruct high-resolution phase images.Using HISTR-SAPM,we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap(i.e.,a full pitch of 330 nm).As the reconstruction averages out laser speckle noise while maintaining high temporal resolution,HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells,such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells.We envision that HISTR-SAPM will broadly benefit research in material science and biology.
基金supported by the National Natural Science Foundation of China(62227818,62105151,62175109,U21B2033,62105156,62361136588)National Key Research and Development Program of China(2022YFA1205002)+7 种基金Leading Technology of Jiangsu Basic Research Plan(BK20192003)Youth Foundation of Jiangsu Province(BK20210338)Biomedical Competition Foundation of Jia-ngsu Province(BE2022847)Key National Industrial Technology Cooperation Foundation of Jiangsu Province(BZ2022039)Fundamental Research Funds for the Central Universities(30920032101,30923010206)Fundamental Scientific Research Business Fee Funds for the Central Universities(2023102001)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201)National Science Center,Poland(2020/37/B/ST7/03629).
文摘The transport of intensity equation(TIE)is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardware.However,existing TIE methods encounter difficulties in decoupling the independent contributions of phase and aberrations to the measurements in the case of unknown pupil function.Additionally,spatially nonuniform and temporally varied aberrations dramatically degrade the imaging performance for long-term research.Hence,it remains a critical challenge to realize the high-throughput quantitative phase imaging(QPI)with aberration correction under partially coherent illumination.To address these issues,we propose a novel method for highthroughput microscopy with annular illumination,termed as transport-of-intensity QPI with aberration correction(TI-AC).By combining aberration correction and pixel super-resolution technique,TI-AC is made compatible with large pixel-size sensors to enable a broader field of view.Furthermore,it surpasses the theoretical Nyquist-Shannon sampling resolution limit,resulting in an improvement of more than two times.Experimental results demonstrate that the half-width imaging resolution can be improved to~345 nm across a 10×field of view of 1.77 mm^(2)(0.4 NA).Given its high-throughput capability for QPI,TI-AC is expected to be adopted in biomedical fields,such as drug discovery and cancer diagnostics.
