Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challeng...Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challenge emerges from the misalignment between the mid-IR light wavelength range and molecular dimensions,culminating in a constrained absorption cross-section and diminished vibrational absorption coefficients(Supplementary data).展开更多
Objective:To gain insight into the potential factors that may cause perceived stress and anxiety in simulation education.Methods:A secondary qualitative analysis study using qualitative thematic synthesis.A local high...Objective:To gain insight into the potential factors that may cause perceived stress and anxiety in simulation education.Methods:A secondary qualitative analysis study using qualitative thematic synthesis.A local higher education institution that conducted pre-registration nursing programs.A total of 189 undergraduate nursing students that were never attained any clinical placement prior to the parent study.Focus group interviews were conducted to collect data that were then transcribed and analyzed through the qualitative thematic synthesis approach to develop themes.Results:Three themes were emerged from the participants’simulation experiences in terms of peoples(the observers vs the observed),actions(the prepared vs the unprepared),and settings(the realism vs the simulation).Conclusions:By considering and reviewing the current design and development of the simulation practice,the findings of this study contribute to the body of knowledge with valuable insights on stress and anxiety that may affect students’learning in simulation.展开更多
The aromatic compounds,including o-xylene,m-xylene,p-xylene,and ethylbenzene,primarily originate from the catalytic reforming of crude oil,and have a wide variety of applications.However,because of similar physical an...The aromatic compounds,including o-xylene,m-xylene,p-xylene,and ethylbenzene,primarily originate from the catalytic reforming of crude oil,and have a wide variety of applications.However,because of similar physical and chemical properties,these compounds are difficult to be identified by gas chromatography(GC)without standard samples.With the development of modern nuclear magnetic resonance(NMR)techniques,NMR has emerged as a powerful and efficient tool for the rapid analysis of complex and crude mixtures without purification.In this study,the parameters of one-dimensional(1D)total correlation spectroscopy(TOCSY)NMR techniques,including 1D selective gradient TOCSY and 1D chemicalshift-selective filtration(CSSF)with TOCSY,were optimized to obtain comprehensive molecular structure information.The results indicate that the overlapped signals in NMR spectra of nonpolar aromatic compounds(including o-xylene,m-xylene,p-xylene and ethylbenzene),polar aromatic compounds(benzyl alcohol,benzaldehyde,benzoic acid),and aromatic compounds with additional conjugated bonds(styrene)can be resolved in 1D TOCSY.More importantly,full molecular structures can be clearly distinguished by setting appropriate mixing time in 1D TOCSY.This approach simplifies the NMR spectra,provides structural information of entire molecules,and can be applied for the analysis of other structural isomers.展开更多
Liquid chromatography hybrid ion trap/time-of-flight mass spectrometry possesses both the MS^n ability of ion trap and the excellent resolution of a time-of-flight and has been widely used to identify drug metabolites...Liquid chromatography hybrid ion trap/time-of-flight mass spectrometry possesses both the MS^n ability of ion trap and the excellent resolution of a time-of-flight and has been widely used to identify drug metabolites and determine trace multi-components in natural products. Collision energy, one of the most important factors in acquiring MS^n information, could be set freely in the range of 10%–400%. Herein, notoginsenosides were chosen as model compounds to build a novel methodology for the collision energy optimization. Firstly, the fragmental patterns of the representatives for the authentic standards of protopanaxadiol-type and protopanaxatriol-type notoginsenosides were obtained based on accurate MS^2 and MS^3 measurements via liquid chromatography hybrid ion trap/time-of-flight mass spectrometry. The extracted ion chromatograms of characteristic product ions of notoginsenosides in Panax Notoginseng Extract were produced under a series of collision energies and compared to screen the optimum collision energies values for MS^2 and MS^3. The results demonstrated that the qualitative capability of liquid chromatography hybrid ion trap/time-of-flight mass spectrometry was greatly influenced by collision energies, and 50% of MS^2 collision energy was found to produce the highest collision-induced dissociation efficiency for notoginsenosides. Addtionally, the highest collision-induced dissociation efficiency appeared when the collision energy was set at 75% in the MS^3 stage.展开更多
The generalized nonlinear Schrdinger equation with parabolic law nonlinearity is studied by using the factorization technique and the method of dynamical systems.From a dynamic point of view,the existence of smooth so...The generalized nonlinear Schrdinger equation with parabolic law nonlinearity is studied by using the factorization technique and the method of dynamical systems.From a dynamic point of view,the existence of smooth solitary wave,kink and anti-kink wave is proved and the sufficient conditions to guarantee the existence of the above solutions in different regions of the parametric space are given.Also,all possible explicit exact parametric representations of the waves are presented.展开更多
Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not cle...Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship （r2, 0.9994-0.9999）, precisions （inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%）, repeatability （RSD, 1.66%-3.67%）, stability （RSD, 1.26%-4.77%） and recovery （95.24%-105.41%, RSD, 0.29%-2.69%）. Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. 3＂he total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.展开更多
A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer（HPLC-ESI-MS/MS） coupled with a photodiode array detector（HPLC-DAD） and principal component analysis（PCA） was ...A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer（HPLC-ESI-MS/MS） coupled with a photodiode array detector（HPLC-DAD） and principal component analysis（PCA） was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri（CP） samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis（CPC） and Cortex Phellodendri Amurensis（CPA）. Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC（58.75 mg/g） was higher than that in CPA（9.16 mg/g）, while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, pahnatine, phellodendrine and magnoflorine contribute most to the above mentioned calssifying . The proposed method oroved to be a useful tool in the aualitv control of Chinese herbal medicines.展开更多
Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on...Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on C. monnieri. Methods Qualitative analysis of coumarins in C. monnieri was detected by UHPLC-ESI-Q-TOF/MS. Quadrupole TOF/MS in either full scan mode or extracted ion mode was used for the qualitative analysis of the constituents. Relative peak area of each component was used for the hierarchical cluster analysis. Results According to UHPLC-ESI-QTOF-MS data, chemical structures of 28 coumarins in the fruits of C. monnieri were identified, including 19 simple coumarins, seven linear coumarins, and two angular coumarins. Among these constituents, ten coumarins were firstly identified in C. monnieri. In addition, hierarchical cluster analysis suggested that C. monnieri from different regions could be classified into four groups, and this clustering was correlated to the distribution significantly, Xuzhou could be regarded as the genuine producing area. Conclusion UHPLC-ESI-Q-TOF-MS is a viable method for qualitative analysis and quality evaluation of coumarins from the fruit of C. monnieri. Coumarins in C. monnieri exists intra-species variance, which indicates significant meaning for the quality control and choice of famous region drug for C. monnieri in the clinic medication.展开更多
Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently...Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).展开更多
We first investigate some basic properties of dynamic equations on time scales,and propose contained curves to describe the jump direction of the discrete points.Then we perform qualitative analysis regarding the plan...We first investigate some basic properties of dynamic equations on time scales,and propose contained curves to describe the jump direction of the discrete points.Then we perform qualitative analysis regarding the planar predator-prey systems on time scales,thereby obtain two theorems of this system.At last,we emulate application examples to discuss the parameters of the system.展开更多
Hydrocarbon prospective zone is a certain layer in a reservoir which is estimated producing oil. The geologists use the qualitative analysis method to find the prospect layers. The research used five variables modeled...Hydrocarbon prospective zone is a certain layer in a reservoir which is estimated producing oil. The geologists use the qualitative analysis method to find the prospect layers. The research used five variables modeled by three fuzzy membership functions and eight rules of fuzzy logic. The rules cause insensitiveness in the working system. This study therefore was conducted by modeling each of input variables into different models using 36 rules. It aims to determine the existence of hydrocarbon prospective zone through a qualitative analysis in a reservoir using fuzzy inference system with Mamdani method. The data were taken from well log data in reservoir ＂X＂. There were some steps in doing this study, including fuzzification, inference system, and defuzzification. The result showed 99 prospect layers from 3000 layers in reservoir ＂X＂ with 97.7% of accuracy.展开更多
This paper makes qualitative analysis to the bounded traveling wave solutions for a kind of nonlinear dispersive-dissipative equation, and considers its solving problem. The relation is investigated between behavior o...This paper makes qualitative analysis to the bounded traveling wave solutions for a kind of nonlinear dispersive-dissipative equation, and considers its solving problem. The relation is investigated between behavior of its solution and the dissipation coefficient. Further, all approximate damped oscillatory solutions when dissipation coefficient is small are presented by utilizing the method of undetermined coefficients according to the theory of rotated vector field in planar dynamical systems. Finally, error estimate is given by establishing the integral equation which reflects the relation between approximate and exact damped oscillatory solutions applying the idea of homogenization principle.展开更多
In this paper, we apply the theory of planar dynamical systems to carry out qualitative analysis for the dynamical system corresponding to B-BBM equation, and obtain global phase portraits under various parameter cond...In this paper, we apply the theory of planar dynamical systems to carry out qualitative analysis for the dynamical system corresponding to B-BBM equation, and obtain global phase portraits under various parameter conditions. Then, the relations between the behaviors of bounded traveling wave solutions and the dissipation coeffiicient μ are investigated. We find that a bounded traveling wave solution appears as a kink profile solitary wave solution when μ is more than the critical value obtained in this paper, while a bounded traveling wave solution appears as a damped oscillatory solution when μ is less than it. Furthermore, we explain the solitary wave solutions obtained in previous literature, and point out their positions in global phase portraits. In the meantime, approximate damped oscillatory solutions are given by means of undetermined coefficients method. Finally, based on integral equations that reflect the relations between the approximate damped oscillatory solutions and the implicit exact damped oscillatory solutions, error estimates for the approximate solutions are presented.展开更多
In this paper a mathematical model of AIDS is investigated.The conditions of the existence of equilibria and local stability of equilibria are given.The existences of transcritical bifurcation and Hopf bifurcation are...In this paper a mathematical model of AIDS is investigated.The conditions of the existence of equilibria and local stability of equilibria are given.The existences of transcritical bifurcation and Hopf bifurcation are also considered.in particular,the conditions for the existence of Hopf bifurcation can be given in terms of the coefficients of the characteristic equation.The method extends the application of the Hopf bifurcation theorem to higher differential equations which occur in biological models,chemical models,and epidemiological models etc.展开更多
In this paper we analyze the qualitative behaviour of the equation ε+q(X) +εX=bp(t), where e is a small parameter.We divide the interval of parameter b into four sets of subintervals,A, B,C and D.For bA,B or D,we di...In this paper we analyze the qualitative behaviour of the equation ε+q(X) +εX=bp(t), where e is a small parameter.We divide the interval of parameter b into four sets of subintervals,A, B,C and D.For bA,B or D,we discuss the different structures of the attractors of the equation and their stabilities.When chaotic phenomena appear,we also estimate the entropy.For bC,the set of bifurcation intervals,we analyze the bifurcating type and get a series of consequences from the results of Newhouse and Palis.展开更多
In the past 30 years,signed directed graph(SDG) ,one of the qualitative simulation technologies,has been widely applied for chemical fault diagnosis.However,SDG based fault diagnosis,as any other qualitative method,ha...In the past 30 years,signed directed graph(SDG) ,one of the qualitative simulation technologies,has been widely applied for chemical fault diagnosis.However,SDG based fault diagnosis,as any other qualitative method,has poor diagnostic resolution.In this paper,a new method that combines SDG with qualitative trend analysis(QTA) is presented to improve the resolution.In the method,a bidirectional inference algorithm based on assumption and verification is used to find all the possible fault causes and their corresponding consistent paths in the SDG model.Then an improved QTA algorithm is used to extract and analyze the trends of nodes on the consis-tent paths found in the previous step.New consistency rules based on qualitative trends are used to find the real causes from the candidate causes.The resolution can be improved.This method combines the completeness feature of SDG with the good diagnostic resolution feature of QTA.The implementation of SDG-QTA based fault diagno-sis is done using the integrated SDG modeling,inference and post-processing software platform.Its application is illustrated on an atmospheric distillation tower unit of a simulation platform.The result shows its good applicability and efficiency.展开更多
Hirsch[1,2] studied the limiting behavior of solutions of competitive or cooperative systems, and showed that ifL is an ω-limit set of a three-dimensional cooperative system, which contains no equilibrium, thenL is a...Hirsch[1,2] studied the limiting behavior of solutions of competitive or cooperative systems, and showed that ifL is an ω-limit set of a three-dimensional cooperative system, which contains no equilibrium, thenL is a nonattracting closed orbit. Smith<sup class='a-plus-plus'>[3]</sup> considered a three-dimensional irreducible competitive system and showed that an ω-limit set containing no equilibrium must be a closed orbit which has a simple Floquet multiplier λ<1, and may be attracting. In this paper we carry out the qualitative analysis of a class of competitive and cooperative systems, and a generalization of the result of Levine<sup class='a-plus-plus'>[4]</sup> is given. The stability problem of closed orbits raised in [5] and [6] is resolved.展开更多
This paper provides a survey on symbolic computational approaches for the analysis of qualitative behaviors of systems of ordinary differential equations,focusing on symbolic and algebraic analysis for the local stabi...This paper provides a survey on symbolic computational approaches for the analysis of qualitative behaviors of systems of ordinary differential equations,focusing on symbolic and algebraic analysis for the local stability and bifurcation of limit cycles in the neighborhoods of equilibria and periodic orbits of the systems,with a highlight on applications to computational biology.展开更多
In order to identify the rock burst hazard in coalmine and thus to give a credible forecast, firstly, analyzed such effect factors as natural geological factors and mining technological conditions based on the investi...In order to identify the rock burst hazard in coalmine and thus to give a credible forecast, firstly, analyzed such effect factors as natural geological factors and mining technological conditions based on the investigation of more than one hundred mine rock burst cases. Secondly, adopted the fault tree analysis （FTA） technology to the mine rock burst hazard identification for the first time and confirmed twelve kinds of basic events, that is, the large mining depth, the burst-orientation coal seams, the solid strata of roof and bottom, near the faults with bigger fall, the folds, the change of seam thickness, other regional tectonics transformation or stress strip, the drilling, blasting and extracting operation, the unscientific extracting methods, the illogical extracting sequence, the residual pillars and the too close distance between the working face and the residual areas or the stopping extracting lines. Moreover, worked out the fault tree of mine rock burst. At last, it made qualitative analysis and quantitative analysis and forecasted the rock burst hazard according to the characteristic of geologic structure and exploitation technology conditions in certain mine of Shandong Province, China, the rock burst accidents happened in the following exploitation validated that it is of feasibility and veracity adopting FTA to identify the mine rock burst hazard.展开更多
Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to...Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to overcome this problem, this study respectively cloned CrylA（B）, BAR, CP4-EPSPS, PAT and RBCL genes into PMD 18-T vector. This constructed plasmid could be used as the standard reference molecule in the qualitative detecting of the exogenous CP4-EPSPS gene, CrylA（B） gene, BAR gene and PAT gene. Identification by double restriction endonuclease digestion and identification by PCR proved that the test result was positive. All of these indicated that the established reference molecules in this study were suitable for the qualitative analysis standard of this GMC detection.展开更多
基金supported by National Natural Science Foundation of China(Grant No.:32301161)the Natural Scientific Foundation of Hunan Province,China(Grant No.:2023JJ60052)+3 种基金the Scientific Research Project of Hunan Provincial Health Commission,China(Grant No.:202112062218,20190161)the Scientific Research Project of Hunan Provincial Department of Education,China(Grant No.:22B0455)the Clinical“4310”Project of the University of South China,China(Grant No.:20224310NHYCG02)the Doctoral Scientific Research Foundation of University of South China,China(Grant No.:200XQD042).
文摘Infrared(IR)spectroscopy,a technique within the realm of molecular vibrational spectroscopy,furnishes distinctive chemical signatures pivotal for both structural analysis and compound identification.A notable challenge emerges from the misalignment between the mid-IR light wavelength range and molecular dimensions,culminating in a constrained absorption cross-section and diminished vibrational absorption coefficients(Supplementary data).
基金supported by School Research Grant of Tung Wah College(SRG210401).
文摘Objective:To gain insight into the potential factors that may cause perceived stress and anxiety in simulation education.Methods:A secondary qualitative analysis study using qualitative thematic synthesis.A local higher education institution that conducted pre-registration nursing programs.A total of 189 undergraduate nursing students that were never attained any clinical placement prior to the parent study.Focus group interviews were conducted to collect data that were then transcribed and analyzed through the qualitative thematic synthesis approach to develop themes.Results:Three themes were emerged from the participants’simulation experiences in terms of peoples(the observers vs the observed),actions(the prepared vs the unprepared),and settings(the realism vs the simulation).Conclusions:By considering and reviewing the current design and development of the simulation practice,the findings of this study contribute to the body of knowledge with valuable insights on stress and anxiety that may affect students’learning in simulation.
基金We thank the Natural Science Foundation of Shanxi Province(202103021224439)National Natural Science Foundation of China(22075308)for financial support.
文摘The aromatic compounds,including o-xylene,m-xylene,p-xylene,and ethylbenzene,primarily originate from the catalytic reforming of crude oil,and have a wide variety of applications.However,because of similar physical and chemical properties,these compounds are difficult to be identified by gas chromatography(GC)without standard samples.With the development of modern nuclear magnetic resonance(NMR)techniques,NMR has emerged as a powerful and efficient tool for the rapid analysis of complex and crude mixtures without purification.In this study,the parameters of one-dimensional(1D)total correlation spectroscopy(TOCSY)NMR techniques,including 1D selective gradient TOCSY and 1D chemicalshift-selective filtration(CSSF)with TOCSY,were optimized to obtain comprehensive molecular structure information.The results indicate that the overlapped signals in NMR spectra of nonpolar aromatic compounds(including o-xylene,m-xylene,p-xylene and ethylbenzene),polar aromatic compounds(benzyl alcohol,benzaldehyde,benzoic acid),and aromatic compounds with additional conjugated bonds(styrene)can be resolved in 1D TOCSY.More importantly,full molecular structures can be clearly distinguished by setting appropriate mixing time in 1D TOCSY.This approach simplifies the NMR spectra,provides structural information of entire molecules,and can be applied for the analysis of other structural isomers.
基金supported by the National Nature Science Foundation of China(Nos.81273589,81374054)the Nature Science Foundation of Jiangsu Province(BK20131311)+2 种基金the fundamental research special fund of China Pharmaceutical University(PT2014 YK0081)Jiangsu Provincial Promotion Foundation for the Key Laboratory of Drug Metabolism and Pharmacokinetics(BM2012012)Jiangsu Key Laboratory of Drug Design and Optimization
文摘Liquid chromatography hybrid ion trap/time-of-flight mass spectrometry possesses both the MS^n ability of ion trap and the excellent resolution of a time-of-flight and has been widely used to identify drug metabolites and determine trace multi-components in natural products. Collision energy, one of the most important factors in acquiring MS^n information, could be set freely in the range of 10%–400%. Herein, notoginsenosides were chosen as model compounds to build a novel methodology for the collision energy optimization. Firstly, the fragmental patterns of the representatives for the authentic standards of protopanaxadiol-type and protopanaxatriol-type notoginsenosides were obtained based on accurate MS^2 and MS^3 measurements via liquid chromatography hybrid ion trap/time-of-flight mass spectrometry. The extracted ion chromatograms of characteristic product ions of notoginsenosides in Panax Notoginseng Extract were produced under a series of collision energies and compared to screen the optimum collision energies values for MS^2 and MS^3. The results demonstrated that the qualitative capability of liquid chromatography hybrid ion trap/time-of-flight mass spectrometry was greatly influenced by collision energies, and 50% of MS^2 collision energy was found to produce the highest collision-induced dissociation efficiency for notoginsenosides. Addtionally, the highest collision-induced dissociation efficiency appeared when the collision energy was set at 75% in the MS^3 stage.
基金Supported by the National Natural Science Foundation of China under Grant No.11461022the Major Natural Science Foundation of Yunnan Province under Grant No.2014FA037
文摘The generalized nonlinear Schrdinger equation with parabolic law nonlinearity is studied by using the factorization technique and the method of dynamical systems.From a dynamic point of view,the existence of smooth solitary wave,kink and anti-kink wave is proved and the sufficient conditions to guarantee the existence of the above solutions in different regions of the parametric space are given.Also,all possible explicit exact parametric representations of the waves are presented.
基金Key Projects in the National Science & Technology Pillar Program during the 11thFive-Year Plan Period (Grant No.2008BAI51B02)National Natural Science Fund Project of China (Grant No. 81202904)
文摘Huang-Lian-Jie-Du-Decoction （HLJDD） has been widely used for the treatment of Alzheimer＇s disease （AD） in clinic. However, the relationship between its chemical profile and neuroprotective bioactivity was not clearly clarified yet. In present study, the water extract of HLJDD and subsequent three polarity fractions divided by different reagents were investigated. A total of 17 chromatographic peaks were confirmed by comparison with standards and their UV, MS spectra. Among them, 11 major compounds were determined by HPLC-DAD method with good linear regression relationship （r2, 0.9994-0.9999）, precisions （inter-day precision RSD, 0.79%-1.07%; intra-day precision RSD, 1.59%-2.10%）, repeatability （RSD, 1.66%-3.67%）, stability （RSD, 1.26%-4.77%） and recovery （95.24%-105.41%, RSD, 0.29%-2.69%）. Furthermore, PC12 cells and primary neurons cells were used for the neuroprotective effective assessment of aforementioned four samples from HLJDD. 3＂he total aqueous extract and n-butanol extract of HLJDD presented more significant effects than the other two parts. According to their quality and quantity determination results, iridoids and alkaloids have a positive correlation with the neuroprotective effectiveness of HLJDD.
基金Supported by the National Natural Science Foundation of China(No30725045)the Foundation of Eleventh Five-Year-Plan of China(No2008ZX09202-002)+1 种基金the Shanghai Leading Academic Discipline Project, China(NoB906)the Scientific Foundation of Shanghai City, China(No07DZ19702)
文摘A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectrometer（HPLC-ESI-MS/MS） coupled with a photodiode array detector（HPLC-DAD） and principal component analysis（PCA） was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri（CP） samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis（CPC） and Cortex Phellodendri Amurensis（CPA）. Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC（58.75 mg/g） was higher than that in CPA（9.16 mg/g）, while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, pahnatine, phellodendrine and magnoflorine contribute most to the above mentioned calssifying . The proposed method oroved to be a useful tool in the aualitv control of Chinese herbal medicines.
基金National Natural Science Foundation of China(No.81202865)
文摘Objective To identify the coumarins constituents in Cnidium monnieri and classify ten samples into three groups and this helpful chemical information could be used for the further pharmacological and clinical study on C. monnieri. Methods Qualitative analysis of coumarins in C. monnieri was detected by UHPLC-ESI-Q-TOF/MS. Quadrupole TOF/MS in either full scan mode or extracted ion mode was used for the qualitative analysis of the constituents. Relative peak area of each component was used for the hierarchical cluster analysis. Results According to UHPLC-ESI-QTOF-MS data, chemical structures of 28 coumarins in the fruits of C. monnieri were identified, including 19 simple coumarins, seven linear coumarins, and two angular coumarins. Among these constituents, ten coumarins were firstly identified in C. monnieri. In addition, hierarchical cluster analysis suggested that C. monnieri from different regions could be classified into four groups, and this clustering was correlated to the distribution significantly, Xuzhou could be regarded as the genuine producing area. Conclusion UHPLC-ESI-Q-TOF-MS is a viable method for qualitative analysis and quality evaluation of coumarins from the fruit of C. monnieri. Coumarins in C. monnieri exists intra-species variance, which indicates significant meaning for the quality control and choice of famous region drug for C. monnieri in the clinic medication.
基金We thank for the funding support from the National Standardization Construction in TCMs of China(No.ZYBZH-Y-HUN-23)National Key Research and Development Projects of China(No.2018YFC1707903)Key Research and Development Projects of Hunan Province(No.2018SK2119).
文摘Objective To explore the major compound in Polygonati Rhizoma(Huang Jing,黄精)for quality control.Methods The major compound was isolated and analyzed by liquid chromatography-mass spectrometry(LC-MS),and subsequently further identified by nuclear magnetic resonance(NMR).Thin layer chromatography(TLC)was optimized based on the previous methods reported in the Chinese Pharmacopeia(2015 edition).Results The major compound was isolated from the natural material and identified as linoleic acid.A high performance liquid chromatography(HPLC)method with robust linearity(R2=0.9997),specificity,precision,stability,repeatability and recovery was developed for linoleic acid determination.TLC chromatogram was improved significantly after optimization for qualitative analysis.Conclusions The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma(Huang Jing,黄精).The levels of linoleic acid vary between species of Polygonati Rhizoma(Huang Jing,黄精),with Polygonatum cyrtonema Hua(Jiang Xing Huang Jing,姜型黄精)showing the highest contents.This study provides valuable information for quality control of Polygonati Rhizoma(Huang Jing,黄精).
基金The paper is supported by the National Natural Science Foundation of China (No.10371135,No.10701037 and No.10571183).
文摘We first investigate some basic properties of dynamic equations on time scales,and propose contained curves to describe the jump direction of the discrete points.Then we perform qualitative analysis regarding the planar predator-prey systems on time scales,thereby obtain two theorems of this system.At last,we emulate application examples to discuss the parameters of the system.
文摘Hydrocarbon prospective zone is a certain layer in a reservoir which is estimated producing oil. The geologists use the qualitative analysis method to find the prospect layers. The research used five variables modeled by three fuzzy membership functions and eight rules of fuzzy logic. The rules cause insensitiveness in the working system. This study therefore was conducted by modeling each of input variables into different models using 36 rules. It aims to determine the existence of hydrocarbon prospective zone through a qualitative analysis in a reservoir using fuzzy inference system with Mamdani method. The data were taken from well log data in reservoir ＂X＂. There were some steps in doing this study, including fuzzification, inference system, and defuzzification. The result showed 99 prospect layers from 3000 layers in reservoir ＂X＂ with 97.7% of accuracy.
基金Supported by the National Natural Science Foundation of China(No.11471215)
文摘This paper makes qualitative analysis to the bounded traveling wave solutions for a kind of nonlinear dispersive-dissipative equation, and considers its solving problem. The relation is investigated between behavior of its solution and the dissipation coefficient. Further, all approximate damped oscillatory solutions when dissipation coefficient is small are presented by utilizing the method of undetermined coefficients according to the theory of rotated vector field in planar dynamical systems. Finally, error estimate is given by establishing the integral equation which reflects the relation between approximate and exact damped oscillatory solutions applying the idea of homogenization principle.
基金supported by Shanghai Leading Academic Discipline Project (No. S30501)Shanghai Natural Science Foundation Project (No. 10ZR1420800)
文摘In this paper, we apply the theory of planar dynamical systems to carry out qualitative analysis for the dynamical system corresponding to B-BBM equation, and obtain global phase portraits under various parameter conditions. Then, the relations between the behaviors of bounded traveling wave solutions and the dissipation coeffiicient μ are investigated. We find that a bounded traveling wave solution appears as a kink profile solitary wave solution when μ is more than the critical value obtained in this paper, while a bounded traveling wave solution appears as a damped oscillatory solution when μ is less than it. Furthermore, we explain the solitary wave solutions obtained in previous literature, and point out their positions in global phase portraits. In the meantime, approximate damped oscillatory solutions are given by means of undetermined coefficients method. Finally, based on integral equations that reflect the relations between the approximate damped oscillatory solutions and the implicit exact damped oscillatory solutions, error estimates for the approximate solutions are presented.
基金This project is supported by the National Science Foundation "Tian Yuan" Terms and LNM Institute of Mechanics Academy of ScienceThis project is supported by the NationalYunnan Province Natural Science Foundation of China
文摘In this paper a mathematical model of AIDS is investigated.The conditions of the existence of equilibria and local stability of equilibria are given.The existences of transcritical bifurcation and Hopf bifurcation are also considered.in particular,the conditions for the existence of Hopf bifurcation can be given in terms of the coefficients of the characteristic equation.The method extends the application of the Hopf bifurcation theorem to higher differential equations which occur in biological models,chemical models,and epidemiological models etc.
文摘In this paper we analyze the qualitative behaviour of the equation ε+q(X) +εX=bp(t), where e is a small parameter.We divide the interval of parameter b into four sets of subintervals,A, B,C and D.For bA,B or D,we discuss the different structures of the attractors of the equation and their stabilities.When chaotic phenomena appear,we also estimate the entropy.For bC,the set of bifurcation intervals,we analyze the bifurcating type and get a series of consequences from the results of Newhouse and Palis.
基金Supported by the Science and Technological Tackling Project of Heilongjiang Province(GB06A106)
文摘In the past 30 years,signed directed graph(SDG) ,one of the qualitative simulation technologies,has been widely applied for chemical fault diagnosis.However,SDG based fault diagnosis,as any other qualitative method,has poor diagnostic resolution.In this paper,a new method that combines SDG with qualitative trend analysis(QTA) is presented to improve the resolution.In the method,a bidirectional inference algorithm based on assumption and verification is used to find all the possible fault causes and their corresponding consistent paths in the SDG model.Then an improved QTA algorithm is used to extract and analyze the trends of nodes on the consis-tent paths found in the previous step.New consistency rules based on qualitative trends are used to find the real causes from the candidate causes.The resolution can be improved.This method combines the completeness feature of SDG with the good diagnostic resolution feature of QTA.The implementation of SDG-QTA based fault diagno-sis is done using the integrated SDG modeling,inference and post-processing software platform.Its application is illustrated on an atmospheric distillation tower unit of a simulation platform.The result shows its good applicability and efficiency.
文摘Hirsch[1,2] studied the limiting behavior of solutions of competitive or cooperative systems, and showed that ifL is an ω-limit set of a three-dimensional cooperative system, which contains no equilibrium, thenL is a nonattracting closed orbit. Smith<sup class='a-plus-plus'>[3]</sup> considered a three-dimensional irreducible competitive system and showed that an ω-limit set containing no equilibrium must be a closed orbit which has a simple Floquet multiplier λ<1, and may be attracting. In this paper we carry out the qualitative analysis of a class of competitive and cooperative systems, and a generalization of the result of Levine<sup class='a-plus-plus'>[4]</sup> is given. The stability problem of closed orbits raised in [5] and [6] is resolved.
基金The work was partially supported by the National Natural Science Foundation of China(12101032,12131004 and 11601023)Ministry of Science and Technology of China(2021YFA1003600)Beijing Natural Science Foundation(1212005).
文摘This paper provides a survey on symbolic computational approaches for the analysis of qualitative behaviors of systems of ordinary differential equations,focusing on symbolic and algebraic analysis for the local stability and bifurcation of limit cycles in the neighborhoods of equilibria and periodic orbits of the systems,with a highlight on applications to computational biology.
文摘In order to identify the rock burst hazard in coalmine and thus to give a credible forecast, firstly, analyzed such effect factors as natural geological factors and mining technological conditions based on the investigation of more than one hundred mine rock burst cases. Secondly, adopted the fault tree analysis （FTA） technology to the mine rock burst hazard identification for the first time and confirmed twelve kinds of basic events, that is, the large mining depth, the burst-orientation coal seams, the solid strata of roof and bottom, near the faults with bigger fall, the folds, the change of seam thickness, other regional tectonics transformation or stress strip, the drilling, blasting and extracting operation, the unscientific extracting methods, the illogical extracting sequence, the residual pillars and the too close distance between the working face and the residual areas or the stopping extracting lines. Moreover, worked out the fault tree of mine rock burst. At last, it made qualitative analysis and quantitative analysis and forecasted the rock burst hazard according to the characteristic of geologic structure and exploitation technology conditions in certain mine of Shandong Province, China, the rock burst accidents happened in the following exploitation validated that it is of feasibility and veracity adopting FTA to identify the mine rock burst hazard.
基金Supported by the Innovative Team Funds of Northeast Agricultural University (CXT004-3-2)Foundation of Heilongjiang Educational Committee (11511030)
文摘Now every laboratory is facing a common problem that is seriously lacking in the standard reference molecule in the GMC detecting research center. Constructing the recombinant plasmid is the urgent demand. In order to overcome this problem, this study respectively cloned CrylA（B）, BAR, CP4-EPSPS, PAT and RBCL genes into PMD 18-T vector. This constructed plasmid could be used as the standard reference molecule in the qualitative detecting of the exogenous CP4-EPSPS gene, CrylA（B） gene, BAR gene and PAT gene. Identification by double restriction endonuclease digestion and identification by PCR proved that the test result was positive. All of these indicated that the established reference molecules in this study were suitable for the qualitative analysis standard of this GMC detection.
