Norovirus(NoV)infection is a major cause of gastroenteritis worldwide.The virus poses great challenges in developing vaccines with broad immune protection due to its genetic and antigenic diversity.To date,there are n...Norovirus(NoV)infection is a major cause of gastroenteritis worldwide.The virus poses great challenges in developing vaccines with broad immune protection due to its genetic and antigenic diversity.To date,there are no approved NoV vaccines for clinical use.Here,we aimed to develop a broad-acting quadrivalent NoV vaccine based on a chimpanzee adenovirus vector,AdC68,carrying the major capsid protein(VP1)of noroviral GI and GII genotypes.Compared to intramuscular(i.m.),intranasal(i.n.),or other prime-boost immunization regimens(i.m.t i.m.,i.m.t i.n.,i.n.t i.m.),AdC68-GI.1-GII.3(E1)-GII.4-GII.17(E3),administered via i.n.t i.n.induced higher titers of serum IgG antibodies and higher IgA antibodies in bronchoalveolar lavage fluid(BALF)and saliva against the four homologous VP1s in mice.It also significantly stimulated the production of blocking antibodies against the four genotypes.In response to re-stimulation with virus-like particles(VLP)-GI.1,VLP-GII.3,VLP-GII.4,and VLP-GII.17,the quadrivalent vaccine administered according to the i.n.t i.n.regimen effectively triggered specific cell-mediated immune responses,primarily characterized by IFN-γsecretion.Furthermore,the preparation of this novel quadrivalent NoV vaccine requires only a single recombinant adenovirus to provide broad preventive immunity against the major GI/GII epidemic strains,making it a promising vaccine candidate for further development.展开更多
目的评价一款四价流感病毒裂解疫苗在3岁及以上人群中的免疫原性和安全性。方法共入组2700例受试者,采用随机、盲法、阳性对照、非劣效试验设计,按2∶1比例随机分配到试验组或对照组。以不同型别血清抗体几何平均滴度(geometric mean ti...目的评价一款四价流感病毒裂解疫苗在3岁及以上人群中的免疫原性和安全性。方法共入组2700例受试者,采用随机、盲法、阳性对照、非劣效试验设计,按2∶1比例随机分配到试验组或对照组。以不同型别血清抗体几何平均滴度(geometric mean titer,GMT)、阳转率、保护率为指标,评估疫苗接种后28 d的免疫原性。收集接种后28 d内不良事件和6个月内严重不良事件,评估疫苗的安全性。结果3岁及以上人群接种疫苗后,试验组H1N1、H3N2、B/Yamagata、B/Victoria型别抗体GMT分别为382.68、463.22、209.27、133.79,GMT比值(95%CI)分别为0.98(0.90~1.07),1.30(1.18~1.42),1.26(1.16~1.35),1.16(1.08~1.24);抗体阳转率分别为81.11%、92.23%、83.72%、88.49%,抗体阳转率率差(95%CI)分别为4.48%(1.20%~7.87%),4.42%(2.02%~7.02%),8.33%(5.07%~11.71%),5.64%(2.82%~8.62%);保护率分别为96.65%、97.79%、94.67%、92.23%,所有免疫原性指标均达到研究假设。试验组和对照组疫苗接种后发生的不良反应发生率分别为15.75%、16.48%(P=0.656);接种后发生的征集性全身不良反应发生率分别为3.51%、3.45%(P=1.000);接种后发生征集性局部不良反应发生率分别为12.63%、13.36%(P=0.626);未发生与疫苗有关的严重不良事件。结论接种1剂试验疫苗免疫原性非劣效于对照疫苗,且接种后安全性良好。展开更多
Ni-rich layered oxides(Ni>80%)with high energy density have become a mainstream cathode material for Li-ion batteries.However,irreversible phase transitions and interface instability are deep-seated challenges in c...Ni-rich layered oxides(Ni>80%)with high energy density have become a mainstream cathode material for Li-ion batteries.However,irreversible phase transitions and interface instability are deep-seated challenges in commercializing Ni-rich materials.This study used a collaborative modification strategy involving doping and coating with quadrivalent elements to construct Ni-rich materials.In particular,introducing tetravalent Zr makes the valence change of Ni(2+to 4+)more accessible to complete spontaneously during the charging and discharging processes,which significantly suppresses the cationic mixing and irreversible phase transition(H2?H3).Combining the strategy of constructing CeO_(2) coatings on the surface and interfacial spinel-like phases improves the Li+diffusion kinetics and interfacial stability.Simultaneously,part of the strongly oxidizing four-valence Ce^(4+)diffuses to the surface layer,further increasing the average valence state of Ni.Therefore,LiNi_(0.83)Co_(0.11)Mn_(0.06)O_(2)(NCM)-Zr@Ce achieves 78.5%outstanding retention at1.0C after 200 cycles within 3.0-4.3 V compared to unmodified NCM with 41.4%retention.The improved cyclic stability can be attributed to the collaborative modification strategy of the quadrivalent elements,which provides an effective synergistic modification strategy for developing high-performance Li-ion battery cathode materials.展开更多
The layered laurylsulfonate intercalated green rust(lauryl-S GR) was synthesized to evaluate the influence of synthesis parameters and aqueous conditions on the adsorption of CeⅣ.The maximum adsorption capacity of 30...The layered laurylsulfonate intercalated green rust(lauryl-S GR) was synthesized to evaluate the influence of synthesis parameters and aqueous conditions on the adsorption of CeⅣ.The maximum adsorption capacity of 305.58 mg/g by lauryl-S GR was predictably obtained.The pseudo-first-order kinetic model was appropriate in fitting the whole uptake process in a weak acid environment.Three isotherm models including Langmuir, Freundlich, and Tempkin were all reliable in depicting the isotherm adsorption process.The maximum monolayer adsorption capacity of lauryl-S GR towards CeⅣ was 315.46 mg/g.Ce species including CeO and Ce_(2)O_(3) besides CeO_(2) were matched in the XPS distribution, directly indicating the reduction reaction brought by FeⅡ in the GR occurred to hydrated CeⅣ ions during the adsorption.Nano-sized Ce particles attached to the lauryl-S GRs after the adsorption experiments were observed in the morphological characterization.Flocculated materials were formed on the surface of the lauryl-S GR at a pH of 7, which further reduced the active sites and disrupted the continuous uptake of CeⅣ to the lauryl-S GR.This study expands the application of GRs and supplies an ideal iron-based material for the construction of the affiliated recovery pathway to the traditional separation of Ce.展开更多
The meiotic behavior of 10 taxa （nine species and one variety） of the genus Pinus was investigated using pollen mother cells （PMCs） to reveal the differentiation among karyotypes. Chromosome spreads were prepared ...The meiotic behavior of 10 taxa （nine species and one variety） of the genus Pinus was investigated using pollen mother cells （PMCs） to reveal the differentiation among karyotypes. Chromosome spreads were prepared by conventional squashing. The meiotic index and the average configuration were higher, whereas the frequency of aberrance （chromosomal bridges, fragments, or micronuclei） was lower, in all 10 taxa compared with other gynmosperms. The meiotic index, average configuration, and frequency of irregularity were found to be uniform among the species. It was shown that the genomes of the Pinus species investigated were highly stable, confirming results of previous mitotic analyses in this genus. However, slight differentiation of homologous chromosomes among genomes was revealed by analysis of meiotic configurations in Pinus nigra var. poiretiana. Quadrivalents were observed in 9.31% of PMCs in this species. This is the first time that quadrivalents have been observed in gymnosperms.展开更多
基金supported by the National Natural Science Foundation of China(82241065,32070926)to D.Z.the Sichuan Science and Technology Program(2023JDRC0090)to H.L.
文摘Norovirus(NoV)infection is a major cause of gastroenteritis worldwide.The virus poses great challenges in developing vaccines with broad immune protection due to its genetic and antigenic diversity.To date,there are no approved NoV vaccines for clinical use.Here,we aimed to develop a broad-acting quadrivalent NoV vaccine based on a chimpanzee adenovirus vector,AdC68,carrying the major capsid protein(VP1)of noroviral GI and GII genotypes.Compared to intramuscular(i.m.),intranasal(i.n.),or other prime-boost immunization regimens(i.m.t i.m.,i.m.t i.n.,i.n.t i.m.),AdC68-GI.1-GII.3(E1)-GII.4-GII.17(E3),administered via i.n.t i.n.induced higher titers of serum IgG antibodies and higher IgA antibodies in bronchoalveolar lavage fluid(BALF)and saliva against the four homologous VP1s in mice.It also significantly stimulated the production of blocking antibodies against the four genotypes.In response to re-stimulation with virus-like particles(VLP)-GI.1,VLP-GII.3,VLP-GII.4,and VLP-GII.17,the quadrivalent vaccine administered according to the i.n.t i.n.regimen effectively triggered specific cell-mediated immune responses,primarily characterized by IFN-γsecretion.Furthermore,the preparation of this novel quadrivalent NoV vaccine requires only a single recombinant adenovirus to provide broad preventive immunity against the major GI/GII epidemic strains,making it a promising vaccine candidate for further development.
文摘目的评价一款四价流感病毒裂解疫苗在3岁及以上人群中的免疫原性和安全性。方法共入组2700例受试者,采用随机、盲法、阳性对照、非劣效试验设计,按2∶1比例随机分配到试验组或对照组。以不同型别血清抗体几何平均滴度(geometric mean titer,GMT)、阳转率、保护率为指标,评估疫苗接种后28 d的免疫原性。收集接种后28 d内不良事件和6个月内严重不良事件,评估疫苗的安全性。结果3岁及以上人群接种疫苗后,试验组H1N1、H3N2、B/Yamagata、B/Victoria型别抗体GMT分别为382.68、463.22、209.27、133.79,GMT比值(95%CI)分别为0.98(0.90~1.07),1.30(1.18~1.42),1.26(1.16~1.35),1.16(1.08~1.24);抗体阳转率分别为81.11%、92.23%、83.72%、88.49%,抗体阳转率率差(95%CI)分别为4.48%(1.20%~7.87%),4.42%(2.02%~7.02%),8.33%(5.07%~11.71%),5.64%(2.82%~8.62%);保护率分别为96.65%、97.79%、94.67%、92.23%,所有免疫原性指标均达到研究假设。试验组和对照组疫苗接种后发生的不良反应发生率分别为15.75%、16.48%(P=0.656);接种后发生的征集性全身不良反应发生率分别为3.51%、3.45%(P=1.000);接种后发生征集性局部不良反应发生率分别为12.63%、13.36%(P=0.626);未发生与疫苗有关的严重不良事件。结论接种1剂试验疫苗免疫原性非劣效于对照疫苗,且接种后安全性良好。
基金financially supported by the Department of Science and Technology of Guangxi Province (Nos.2022JBGS004,AB21220027,AD19110090 and AD19110077)the National Natural Science Foundation of China (Nos.21805055 and12172096)+2 种基金Guangxi Natural Science Foundation (Nos.2020GXNSFAA159059 and 2020GXNSFAA159037)Guangxi Key Laboratory of Manufacturing Systems Foundation (No.20-065-40-005Z)the Engineering Research Center Foundation of Electronic Information Materials and Devices (No.EIMD-AA202005)。
文摘Ni-rich layered oxides(Ni>80%)with high energy density have become a mainstream cathode material for Li-ion batteries.However,irreversible phase transitions and interface instability are deep-seated challenges in commercializing Ni-rich materials.This study used a collaborative modification strategy involving doping and coating with quadrivalent elements to construct Ni-rich materials.In particular,introducing tetravalent Zr makes the valence change of Ni(2+to 4+)more accessible to complete spontaneously during the charging and discharging processes,which significantly suppresses the cationic mixing and irreversible phase transition(H2?H3).Combining the strategy of constructing CeO_(2) coatings on the surface and interfacial spinel-like phases improves the Li+diffusion kinetics and interfacial stability.Simultaneously,part of the strongly oxidizing four-valence Ce^(4+)diffuses to the surface layer,further increasing the average valence state of Ni.Therefore,LiNi_(0.83)Co_(0.11)Mn_(0.06)O_(2)(NCM)-Zr@Ce achieves 78.5%outstanding retention at1.0C after 200 cycles within 3.0-4.3 V compared to unmodified NCM with 41.4%retention.The improved cyclic stability can be attributed to the collaborative modification strategy of the quadrivalent elements,which provides an effective synergistic modification strategy for developing high-performance Li-ion battery cathode materials.
基金supported by the China Postdoctoral Science Foundation (No.2020M681774)the Natural Science Foundation of the Jiangsu Higher Education institutions of China (No.20KJB490001)the University of South China (No.190XQ073)。
文摘The layered laurylsulfonate intercalated green rust(lauryl-S GR) was synthesized to evaluate the influence of synthesis parameters and aqueous conditions on the adsorption of CeⅣ.The maximum adsorption capacity of 305.58 mg/g by lauryl-S GR was predictably obtained.The pseudo-first-order kinetic model was appropriate in fitting the whole uptake process in a weak acid environment.Three isotherm models including Langmuir, Freundlich, and Tempkin were all reliable in depicting the isotherm adsorption process.The maximum monolayer adsorption capacity of lauryl-S GR towards CeⅣ was 315.46 mg/g.Ce species including CeO and Ce_(2)O_(3) besides CeO_(2) were matched in the XPS distribution, directly indicating the reduction reaction brought by FeⅡ in the GR occurred to hydrated CeⅣ ions during the adsorption.Nano-sized Ce particles attached to the lauryl-S GRs after the adsorption experiments were observed in the morphological characterization.Flocculated materials were formed on the surface of the lauryl-S GR at a pH of 7, which further reduced the active sites and disrupted the continuous uptake of CeⅣ to the lauryl-S GR.This study expands the application of GRs and supplies an ideal iron-based material for the construction of the affiliated recovery pathway to the traditional separation of Ce.
基金supported by the National Natural Science Foundation of China(GrantNo.30121003)
文摘The meiotic behavior of 10 taxa （nine species and one variety） of the genus Pinus was investigated using pollen mother cells （PMCs） to reveal the differentiation among karyotypes. Chromosome spreads were prepared by conventional squashing. The meiotic index and the average configuration were higher, whereas the frequency of aberrance （chromosomal bridges, fragments, or micronuclei） was lower, in all 10 taxa compared with other gynmosperms. The meiotic index, average configuration, and frequency of irregularity were found to be uniform among the species. It was shown that the genomes of the Pinus species investigated were highly stable, confirming results of previous mitotic analyses in this genus. However, slight differentiation of homologous chromosomes among genomes was revealed by analysis of meiotic configurations in Pinus nigra var. poiretiana. Quadrivalents were observed in 9.31% of PMCs in this species. This is the first time that quadrivalents have been observed in gymnosperms.
