Ethnopharmacological relevance: Aqarqarha (Anacyclus pyrethrum) DC root has long been used as a traditional an-tiepileptic remedy in Unani system of medicine over centuries. Aim of the Study: To rationalize the ethnom...Ethnopharmacological relevance: Aqarqarha (Anacyclus pyrethrum) DC root has long been used as a traditional an-tiepileptic remedy in Unani system of medicine over centuries. Aim of the Study: To rationalize the ethnomedical claim and screen for anxiolytic and neurotoxicity profile of ethanolic extract of Aqarqarha (Anacyclus pyrethrum) root (APE). Materials and Methods: The anticonvulsant and anxiolytic potential of APE (100-800 mg/kg) was evaluated against Pentylenetetrazole (PTZ), Bicuculline (BCL), Increasing current electroshock (ICES) and Elevated plus maze(EPM) models. Rotarod test was employed as neurotoxicity model including an additional higher dose (1600 mg/kg). Results: The APE showed significant anticonvulsant activity (p i.p.) in a dose-dependent manner but against BCL (30 mg/kg, i.p.) at the dose 800 mg/kg only (p 0.05). The extract also showed anxiolytic behaviour in EPM (p Conclusions: The results suggested significant anticonvulsant activity of APE against PTZ and BCL but failure against ICES. Moreover, APE also exhibited anxiolytic potential without any evidence of neurotoxicity at the effective dose level. We concluded that anticonvulsant effect of APE is probably mediated by enhancing GABAergic neurotransmission.展开更多
Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)...Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)C NMR,^(1)H-^(1)H COSY,HSQC,HMBC and ROESY),among which the stereostructure of compound 4 was determined by calcu-lated ECD.Furthermore,compounds 1-4 were evaluated for their aphidicidal activities.The insecticidal assay results showed that 1-4 exhibited moderate aphidicidal activities at the concentration of 0.1 mg/mL with the 24 h mortality rates ranging from 10.58 to 52.98%.Among them,pyrethrin D(2)showed the highest aphidicidal activity,with the 24 h mortality rate of 52.98%,which was slightly lower than the positive control(pyrethrin II,83.52%).展开更多
目的 研究阿纳其根醇提取物(ethanol extract of the root of anacyclus pyrethrum,EEAP)对高脂饮食小鼠血糖及其对肠道菌群的影响。方法 将48只C57BL/6N雄性小鼠按照随机数字表法分为对照组,模型组,低、中、高剂量EEAP组及二甲双胍组,...目的 研究阿纳其根醇提取物(ethanol extract of the root of anacyclus pyrethrum,EEAP)对高脂饮食小鼠血糖及其对肠道菌群的影响。方法 将48只C57BL/6N雄性小鼠按照随机数字表法分为对照组,模型组,低、中、高剂量EEAP组及二甲双胍组,每组各8只。低、中、高剂量EEAP组小鼠分别给予100、200、400mg/kg的EEAP,二甲双胍组小鼠给予二甲双胍280mg/kg,对照组和模型组均给予等量蒸馏水,每天灌胃1次,连续12周。除对照组小鼠饮食采用正常饲料外,其余各组小鼠均采用高脂饲料。使用血糖试纸检测各组小鼠空腹和随机血糖,采用酶联免疫吸附试验试剂盒检测小鼠炎性细胞因子水平,通过16S rDNA实时荧光定量聚合酶链反应检测肠道菌群。结果 在第12周时,与模型组比较,低、中、高剂量EEAP组和二甲双胍组小鼠体质量均明显下降。与模型组比较,高剂量EEAP组和二甲双胍组小鼠空腹血糖和随机血糖明显降低。与模型组比较,中、高剂量EEAP组和二甲双胍组小鼠肠道中嗜黏蛋白阿克曼菌和普拉梭菌丰度明显升高,而脱硫弧菌丰度明显降低。结论 EEAP可改善高脂饮食诱导小鼠血糖紊乱,其机制可能与调节肠道菌群、抑制炎性反应有关。展开更多
文摘Ethnopharmacological relevance: Aqarqarha (Anacyclus pyrethrum) DC root has long been used as a traditional an-tiepileptic remedy in Unani system of medicine over centuries. Aim of the Study: To rationalize the ethnomedical claim and screen for anxiolytic and neurotoxicity profile of ethanolic extract of Aqarqarha (Anacyclus pyrethrum) root (APE). Materials and Methods: The anticonvulsant and anxiolytic potential of APE (100-800 mg/kg) was evaluated against Pentylenetetrazole (PTZ), Bicuculline (BCL), Increasing current electroshock (ICES) and Elevated plus maze(EPM) models. Rotarod test was employed as neurotoxicity model including an additional higher dose (1600 mg/kg). Results: The APE showed significant anticonvulsant activity (p i.p.) in a dose-dependent manner but against BCL (30 mg/kg, i.p.) at the dose 800 mg/kg only (p 0.05). The extract also showed anxiolytic behaviour in EPM (p Conclusions: The results suggested significant anticonvulsant activity of APE against PTZ and BCL but failure against ICES. Moreover, APE also exhibited anxiolytic potential without any evidence of neurotoxicity at the effective dose level. We concluded that anticonvulsant effect of APE is probably mediated by enhancing GABAergic neurotransmission.
基金the Key Research and Development Program of Yunnan Province,China(202003AD 150006)the Cooperation Project with DR PLANT Company(2023).
文摘Four undescribed pyrethrins C-F(1-4)as well as four known pyrethrins(5-8)were isolated from seeds of Pyrethrum cinerariifolium Trev.The structures of compounds 1-4 were elucidated by UV,HRESIMS,and NMR(^(1)H and ^(13)C NMR,^(1)H-^(1)H COSY,HSQC,HMBC and ROESY),among which the stereostructure of compound 4 was determined by calcu-lated ECD.Furthermore,compounds 1-4 were evaluated for their aphidicidal activities.The insecticidal assay results showed that 1-4 exhibited moderate aphidicidal activities at the concentration of 0.1 mg/mL with the 24 h mortality rates ranging from 10.58 to 52.98%.Among them,pyrethrin D(2)showed the highest aphidicidal activity,with the 24 h mortality rate of 52.98%,which was slightly lower than the positive control(pyrethrin II,83.52%).
文摘目的 研究阿纳其根醇提取物(ethanol extract of the root of anacyclus pyrethrum,EEAP)对高脂饮食小鼠血糖及其对肠道菌群的影响。方法 将48只C57BL/6N雄性小鼠按照随机数字表法分为对照组,模型组,低、中、高剂量EEAP组及二甲双胍组,每组各8只。低、中、高剂量EEAP组小鼠分别给予100、200、400mg/kg的EEAP,二甲双胍组小鼠给予二甲双胍280mg/kg,对照组和模型组均给予等量蒸馏水,每天灌胃1次,连续12周。除对照组小鼠饮食采用正常饲料外,其余各组小鼠均采用高脂饲料。使用血糖试纸检测各组小鼠空腹和随机血糖,采用酶联免疫吸附试验试剂盒检测小鼠炎性细胞因子水平,通过16S rDNA实时荧光定量聚合酶链反应检测肠道菌群。结果 在第12周时,与模型组比较,低、中、高剂量EEAP组和二甲双胍组小鼠体质量均明显下降。与模型组比较,高剂量EEAP组和二甲双胍组小鼠空腹血糖和随机血糖明显降低。与模型组比较,中、高剂量EEAP组和二甲双胍组小鼠肠道中嗜黏蛋白阿克曼菌和普拉梭菌丰度明显升高,而脱硫弧菌丰度明显降低。结论 EEAP可改善高脂饮食诱导小鼠血糖紊乱,其机制可能与调节肠道菌群、抑制炎性反应有关。
