The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation c...The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition.During optimization process,maximum pyocyanin production(92.12μg/ml)was obtained with carbon source mannitole,nitrogen source peptone,inorganic salt NaCl and metal ion FeSO_(4).Plackett Burman design and Response Surface Methodology(RSM)showed peptone,NaCl and KH_(2)PO_(4)are significant variable in the production of pyocyanin pigment.The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment.The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms.Thus,present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.展开更多
Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance ...Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance wearable sensors to offer prompt feedback.Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously,which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.Therefore,improving materials and analysis system accuracy is essential.This article introduces the first example of a flexible array capable of detecting pyocyanin,a bacterial virulence factor,while correcting dynamic pH fluctuations.We demonstrate that this combined sensor enhances accuracy by mitigating the impact of pH variability on pyocyanin sensor response.Customized screen-printable inks were developed to enhance analytical performance.The analytical performances of two sensitive sensor systems(i.e.,fully-printed porous graphene/multiwalled carbon nanotube(CNT)and polyaniline/CNT composites for pyocyanin and pH sensors)are evaluated.Partial least square regression is employed to analyze nonzero-order data arrays from square wave voltammetric and potentiometric measurements of pyocyanin and pH sensors to establish a predictive model for pyocyanin concentration in complex fluids.This sensitive and effective strategy shows potential for personalized applications due to its affordability,ease of use,and ability to adjust for dynamic pH changes.展开更多
This study evaluated the bacterial toxicity of lanthanum oxide micron and nano sized particles using shake flask method against gram-positive （Staphylococcus aureus） and gram-negative （Escherichia coli, Pseudomonas...This study evaluated the bacterial toxicity of lanthanum oxide micron and nano sized particles using shake flask method against gram-positive （Staphylococcus aureus） and gram-negative （Escherichia coli, Pseudomonas aeruginosa） bacteria. Particle size, morphology and chemical composition were determined using scanning electron microscopy （SEM） and energy dispersive X-ray spectroscopy （EDS）. Results indicated that lanthanum oxide nanoparticles showed antimicrobial activity against Staphylococcus aureus, but not against Escherichia coli and Pseudomonas aeruginosa. It was speculated that lanthanum oxide produced this effect by interacting with the gram-positive bacterial cell wall. Furthermore, lanthanum oxide bulk particles were fotmd to enhance the pyocyanin pigment production in Pseudomonas aeruginosa.展开更多
文摘The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition.During optimization process,maximum pyocyanin production(92.12μg/ml)was obtained with carbon source mannitole,nitrogen source peptone,inorganic salt NaCl and metal ion FeSO_(4).Plackett Burman design and Response Surface Methodology(RSM)showed peptone,NaCl and KH_(2)PO_(4)are significant variable in the production of pyocyanin pigment.The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment.The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms.Thus,present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.
基金the Talent Management Project of Prince of Songkla University
文摘Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids.Wound diagnosis remains challenging,necessitating suitable materials for high-performance wearable sensors to offer prompt feedback.Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously,which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.Therefore,improving materials and analysis system accuracy is essential.This article introduces the first example of a flexible array capable of detecting pyocyanin,a bacterial virulence factor,while correcting dynamic pH fluctuations.We demonstrate that this combined sensor enhances accuracy by mitigating the impact of pH variability on pyocyanin sensor response.Customized screen-printable inks were developed to enhance analytical performance.The analytical performances of two sensitive sensor systems(i.e.,fully-printed porous graphene/multiwalled carbon nanotube(CNT)and polyaniline/CNT composites for pyocyanin and pH sensors)are evaluated.Partial least square regression is employed to analyze nonzero-order data arrays from square wave voltammetric and potentiometric measurements of pyocyanin and pH sensors to establish a predictive model for pyocyanin concentration in complex fluids.This sensitive and effective strategy shows potential for personalized applications due to its affordability,ease of use,and ability to adjust for dynamic pH changes.
文摘This study evaluated the bacterial toxicity of lanthanum oxide micron and nano sized particles using shake flask method against gram-positive （Staphylococcus aureus） and gram-negative （Escherichia coli, Pseudomonas aeruginosa） bacteria. Particle size, morphology and chemical composition were determined using scanning electron microscopy （SEM） and energy dispersive X-ray spectroscopy （EDS）. Results indicated that lanthanum oxide nanoparticles showed antimicrobial activity against Staphylococcus aureus, but not against Escherichia coli and Pseudomonas aeruginosa. It was speculated that lanthanum oxide produced this effect by interacting with the gram-positive bacterial cell wall. Furthermore, lanthanum oxide bulk particles were fotmd to enhance the pyocyanin pigment production in Pseudomonas aeruginosa.
