BACKGROUND We have innovatively amalgamated membrane blood purification and centrifugal blood cell separation technologies to address the limitations of current artificial liver support(ALS)models,and develop a versat...BACKGROUND We have innovatively amalgamated membrane blood purification and centrifugal blood cell separation technologies to address the limitations of current artificial liver support(ALS)models,and develop a versatile plasma purification system(VPPS)through centrifugal plasma separation.AIM To investigate the influence of VPPS on long-term rehospitalization and mortality rates among patients with acute-on-chronic liver failure(ACLF).METHODS This real-world,prospective study recruited inpatients diagnosed with ACLF from the Second Xiangya Hospital of Central South University between October 2021 and March 2024.Patients were categorized into the VPPS and non-VPPS groups based on the distinct ALS models administered to them.Self-administered questionnaires,clinical records,and self-reported data served as the primary methods for data collection.The laboratory results were evaluated at six distinct time points.All patients were subjected to follow-up assessments for>12 months.Kaplan-Meier survival analyses and Cox proportional hazards models were used to evaluate the risks of hospitalization and mortality during the follow-up period.RESULTS A cohort of 502 patients diagnosed with ACLF was recruited,with 260 assigned to the VPPS group.On comparing baseline characteristics,the VPPS group exhibited a significantly shorter length of stay,higher incidence of spontaneous peritonitis and pulmonary aspergillosis compared to the non-VPPS group(P<0.05).Agehazard ratio(HR=1.142,95%CI:1.01-1.23,P=0.018),peritonitis(HR=2.825,95%CI:1.07-6.382,P=0.026),albumin(HR=0.67,95%CI:0.46-0.942,P=0.023),total bilirubin(HR=1.26,95%CI:1.01-3.25,P=0.021),international normalized ratio(HR=1.97,95%CI:1.21-2.908,P=0.014),and VPPS/non-VPPS(HR=3.24,95%CI:2.152-4.76,P<0.001)were identified as significant independent predictors of mortality in both univariate and multivariate analyses throughout the follow-up period.Kaplan-Meier survival analyses demonstrated significantly higher rehospitalization and mortality rates in the non-VPPS group compared to the VPPS group during follow-up of≥2 years(log-rank test,P<0.001).CONCLUSION These findings suggest that VPPS is safe and has a positive influence on prognostic outcomes in patients with ACLF.展开更多
[ Objective ] The aim was to study the influence of filamentous algae on the process of water ecological purification. [ Method ] The occurrence mechanism of filamentous algae and its ecological system were summarized...[ Objective ] The aim was to study the influence of filamentous algae on the process of water ecological purification. [ Method ] The occurrence mechanism of filamentous algae and its ecological system were summarized and analyzed. Considering the ecological purification in north- ern Jiangsu, the occurrence and prevention of filamentous algae in water, the method to prevent filamentous algae in polluted water was discussed. [ Result] The results showed that by measures of improving planting density, regular harvesting, and water flow state control before the filamentous algae blooming period, together with improving local pH value, light interference and ecological control during the blooming period, can effectively control the filamentous algae blooming. [ Condusion] The study of the happening mechanism of filamentous algae provided theoretical references and the technical basis in the work of filamentous algae prevention and control.展开更多
It is of great importance to explore a creative route to improve the degradation e ciency of organic pollutants in wastewater.Herein,we construct a unique hybrid system by combining self-powered triboelectric nanogene...It is of great importance to explore a creative route to improve the degradation e ciency of organic pollutants in wastewater.Herein,we construct a unique hybrid system by combining self-powered triboelectric nanogenerator(TENG)with carbon dots-TiO_(2)sheets doped three-dimensional graphene oxide photocatalyst(3 DGA@CDs-TNs),which can significantly enhance the degradation e ciency of brilliant green(BG)and direct blue 5 B(DB)owing to the powerful interaction of TENG and 3 DGA@CDs-TNs photocatalyst.The power output of TENG can be applied for wastewater purification directly,which exhibits a selfpowered electrocatalytic technology.Furthermore,the results also verify that TENG can replace conventional electric catalyst to remove pollutants e ectively from wastewater without any consumption.Subsequently,the unstable fragments and the plausible removal pathways of the two pollutants are proposed.Our work sheds light on the development of e cient and sustainable TENG/photocatalyst system,opening up new opportunities and possibilities for comprehensive utilization of random energy.展开更多
A garbage incineration power plant is proposed to be built somewhere. Due to local environmental capacity and local policy,the demand on the flue gas emission indicators of the project is high,but the conventional pur...A garbage incineration power plant is proposed to be built somewhere. Due to local environmental capacity and local policy,the demand on the flue gas emission indicators of the project is high,but the conventional purification process of flue gas produced during waste incineration（ SNCR denitrification ＋dry/semi-dry deacidification ＋ adsorption of dioxins and heavy metals by activated carbon ＋ bag dusting） has been unable to meet this requirement,and the newly added wet deacidification and SCR denitrification processes can meet this requirement. The flue gas purification process was optimized,and two feasible schemes were compared to choose the better one.展开更多
Pitaya is an important perennial herbaceous fruit tree.The color of fruit determines pitaya nutritive(and attractive)value,which is considered as an important objective in breeding improvement.In this study,we reporte...Pitaya is an important perennial herbaceous fruit tree.The color of fruit determines pitaya nutritive(and attractive)value,which is considered as an important objective in breeding improvement.In this study,we reported the first telomere-to-telomere(T2T)gap-free genome of“Shuangse No.1”pitaya(Hylocereus polyrhizus;red peel).Two high-quality genomes for“Dahong”(H.polyrhizus;red peel)and“Honghuaqinglong”(H.stenopterus;stay-green)were further assembled,aiming to explore the genetic diversity of pitaya genomes.In further analysis,we noticed a high proportion of viral contamination in pitaya tissues,which hindered the efficient utilization of transcriptomic data.To address this issue,we analyzed 111 pitaya transcriptome data from different geographic regions to characterize and separate viral components.Then we developed an efficient,novel,and universal transcript purification system for pitaya transcriptomes by applying it to 27 samples from different tissues and species,thereby enhancing the utility for transcriptomic and broader biological research.Combining the purified transcriptomic data with comparative genomic analyses,we identified HuERF72,a transcription factor(TF)that potentially regulates chlorophyll degradation in pitaya.Interaction assays and plant transformation elucidated that HuERF72 acts as a repressive TF by directly binding to the promoter of HuSGR1,a key structural gene in the chlorophyll degradation pathway.This study provides high-quality genomic resources and novel methodologies for molecular investigations in pitaya.Additionally,the proposed regulatory network advances our understanding of the transcriptional regulatory mechanisms underlying chlorophyll degradation,offering valuable insights into the genetic improvement of pitaya.展开更多
The artificial liver blood purification system(ALBPS),referred to as the artificial liver system,has been extensively used for treatment of patients with liver failure or other organ failures,and its efficacy has been...The artificial liver blood purification system(ALBPS),referred to as the artificial liver system,has been extensively used for treatment of patients with liver failure or other organ failures,and its efficacy has been well-documented.To further standardize the clinical application of ALBPS,the Liver Failure and Artificial Liver Group,Chinese Society of Infectious Diseases,Chinese Medical Association and other institutions invited experts to update the 2016 version of ALBPS guidelines and develop the Guidelines on Application of Artificial Liver Blood Purification System(version 2023).In line with the latest international research advancements,this updated version compre-hensively elaborates on various aspects of ALBPS,including treatment mode selection,anticoagulation program implementation,nursing and operational procedures,indications and relative contraindications,common complications and their management,as well as management regulations of the system.These guidelines will provide guidance for the effective application of ALBPS for treatment of critically ill patients.展开更多
The establishment of water circulation aquaculture system realized the hi- erarchical use of nitrogen, phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource. H...The establishment of water circulation aquaculture system realized the hi- erarchical use of nitrogen, phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource. However, no research has been reported on the detailed calculation of the relationship between the area of aquaculture pond and purification pond. In this study, referring to the absorption ability of aquatic plants to pollutants in aquaculture wastewater and pollutant generation and discharge coefficient in aquaculture pond, based on the general rules of water quality management in freshwater aquaculture system, a calculation mode was es- tablished to investigate the relationship between the area of aquaculture pond and purification pond in freshwater recirculation aquaculture system, which was feasible to explain related cases and would provide theoretical basis to reduce the economic costs in the construction of water circulation aquaculture system and realize the bal- ance between the ecological benefits and the economic benefits.展开更多
High-concentration oil mists can cause serious health problems to workers,which are generally mitigated by ventilation and purification systems.However,the coupling relationship between these systems is not clear.In t...High-concentration oil mists can cause serious health problems to workers,which are generally mitigated by ventilation and purification systems.However,the coupling relationship between these systems is not clear.In this study,the effects of purifier outlet direction,purification air volume,installation height,and purification efficiency on indoor oil mist distribution were investigated by numerical simulation using an actual machining factory.The mitigation of oil mist in various combinations of ventilation and purification systems was also discussed.The results show that the outlet direction of the purifier has a great influence on the distribution of oil mist in the factory,and the maximum difference of oil mist concentration in the breathing zone under different orientations is 17%.The best purifier outlet direction is vertically upward.When the purifier outlet direction is upward,a larger purification air volume is beneficial for contaminant removal,and a lower purifier exhaust installation height is helpful for oil mist discharge from the bottom of the factory and reducing the concentration of oil mist in the breathing area.The oil mist concentration of purifier exhaust increases from 0 to 2 mg/m^(3) and the oil mist concentration in the breathing zone increases by 67%.The combined system of purification system with the roof exhaust system and displacement ventilation system has the optimal pollution removal efficiency and the lowest concentration of oil mist in the breathing zone compared to other systems.The research results can provide a reference for the design,installation,and operation of ventilation and purification systems in machining factories.展开更多
Enterovirus type 71（EV71） causes severe hand-foot-and-mouth disease （HFMD） resulting in hundreds of deaths of children every year; However, currently, there is no effective treatment for EV71. In this study, the E...Enterovirus type 71（EV71） causes severe hand-foot-and-mouth disease （HFMD） resulting in hundreds of deaths of children every year; However, currently, there is no effective treatment for EV71. In this study, the EV71 poly-protein （EV71-P1 protein） gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GSll5. The EV71 P1 protein with a molecular weight of 100 kD was produced and secreted into the medium. The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%. The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits. We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.展开更多
A system of polar ordered resins was established for purification of chlorogenic acid in Flos Lonicerae. It was composed of three reversed phase resins, AB-8, DM-130 and NKA-9, representative for their gradually incre...A system of polar ordered resins was established for purification of chlorogenic acid in Flos Lonicerae. It was composed of three reversed phase resins, AB-8, DM-130 and NKA-9, representative for their gradually increased polarity and selectivity. A method of RP-HPLC was used for determination of chlorogenic acid. And the performance of adsorption and desorption for chlorogenic acid with the system of polar ordered resins was studied. Furthermore, the effects of concentration, pH and flow rate of the adsorbate on adsorption ability were researched. It is indicated that the optimum parameters for chlorogenic acid are as follows: pH 3.5 with a flow rate of 2.5 BVh, the concentration of extract solution at 0.50, 0.40, 0.30 gL respectively for the adsorptive operation twice, and 6.93, 8.66, 10.39 molL ethanol used as gradient eluants. The purity of resulted product of chlorogenic acid arrives 70.20% with yield of 89.79%. With simple procedures, low costs and high purity product, the method of system of polar ordered resins followed by sequential reversed phase separations can be used to refine the chlorogenic acid in the extraction of Flos Lonicerae.展开更多
We present a self-error-rejecting multipartite entanglement purification protocol(MEPP)for N-electron-spin entangled states,resorting to the single-side cavity-spin-coupling system.Our MEPP has a high efficiency conta...We present a self-error-rejecting multipartite entanglement purification protocol(MEPP)for N-electron-spin entangled states,resorting to the single-side cavity-spin-coupling system.Our MEPP has a high efficiency containing two steps.One is to obtain high-fidelity N-electron-spin entangled systems with error-heralded parity-check devices(PCDs)in the same parity-mode outcome of three electron-spin pairs,as well as M-electron-spin entangled subsystems(2≤M<N)in the different parity-mode outcomes of those.The other is to regain the N-electron-spin entangled systems from Melectron-spin entangled states utilizing entanglement link.Moreover,the quantum circuits of PCDs make our MEPP works faithfully,due to the practical photon-scattering deviations from the finite side leakage of the microcavity,and the limited coupling between a quantum dot and a cavity mode,converted into a failed detection in a heralded way.展开更多
The Curaua. （Ananaserectifolius LB Smith） is a species belonging to the family Bromeliaceae. Occurs in the states of Para, Amazonas, Amapa, Acre, Mato Grosso and Goia s, has two varieties, purple and white, and its ...The Curaua. （Ananaserectifolius LB Smith） is a species belonging to the family Bromeliaceae. Occurs in the states of Para, Amazonas, Amapa, Acre, Mato Grosso and Goia s, has two varieties, purple and white, and its fibers are used in automotive and textile industries due to its strength, softness and lightweight. Currently, only the fiber is used in industry, the rest is considered waste However, this residue contains compounds with important properties to be discovered and studied. An enzyme complex found in this residue is bromelain, a group of proteolysis＇ enzymes with application in several areas such as in the food, pharmaceutical and cosmetics industries. Today, its use is aiming the pharmaceutical industry, the production of ointments, gels, creams and lotions because they offer a wide range of therapeutic efficacies： antiedemas, anti-inflammatory, antithrombotic and fibrinolytic activities. This work studied the enzyme purification and recovery presented in the leaves of Curaua by means of an ATPS （aqueous two-phase system） PEG 4000/potassium phosphate. The protein content was measured by Bradford reagent and the enzymatic activity was measured by the Biuret reagent. Batch assays were performed aiming the enzyme extraction and recuperation, using the partition coefficient as indicator. It was used pH 7.0, 8.0 and 9.0, varying the proportional composition between the polymeric and the saline phases （tie-lines）. The white variety of Curaua. was used. The best purification factor was 4.53 and about 113.04 μg/mL of total protein measured by Bradford.展开更多
Objective:To study the shock index of patients with sepsis after continuous blood purification (CBP) treatment and its relationship with systemic inflammatory response syndrome and immune response.Methods:A total of 8...Objective:To study the shock index of patients with sepsis after continuous blood purification (CBP) treatment and its relationship with systemic inflammatory response syndrome and immune response.Methods:A total of 88 patients with sepsis who underwent continuous blood purification treatment in our hospital between June 2012 and May 2016 were chosen as research subjects, shock index (SI) was compared before and after the treatment, and according to the level of SI after treatment, all patients were divided into shock group (n=27) with SI>0.5 points and no shock group (n=61) with SI≤0.5 points. Serum contents of inflammatory mediators, Th1/Th2 cellular immunity indexes, immunoglobulin and complement were compared between two groups of patients after treatment.Results: The level of SI in patients with sepsis was significantly lower than that before treatment. Serum contents of inflammatory mediators PCT, CRP and HMGB1 in no shock group were lower than those in shock group, contents of Th1 cytokines IL-2 and IFN-γ were higher than those in shock group, contents of Th2 cytokines IL-10 and IL-13 were lower than those in shock group, and contents of IgG, IgM, IgA, C3 and C4 were higher than those in shock group.Conclusion: The level of SI decreases in the patients with sepsis after CBP treatment, and SI level is directly correlated with the systemic inflammatory response syndrome and immune response levels.展开更多
We present a faithful and efficient hyperentanglement purification protocol(hyper-EPP)for nonlocal two-photon systems in spatial-polarization-time-bin hyperentangled Bell states.As the single-photon detectors can dete...We present a faithful and efficient hyperentanglement purification protocol(hyper-EPP)for nonlocal two-photon systems in spatial-polarization-time-bin hyperentangled Bell states.As the single-photon detectors can detect and herald the undesirable properties caused by side leakage and finite coupling strength,the parity-check gates and swap gates of our hyper-EPP in the spatial,polarization and time-bin mode degrees of freedom(DoFs)work faithfully.The qubit-flip errors in photon systems in three DoFs can be corrected effectively with the faithful parity-check gates and the photon pairs can be reused to distill high-fidelity ones by introducing the faithful swap gates,which greatly increases the efficiency of our hyper-EPP.Further,the maximal hyperentanglement can be obtained in principle by operating multiple rounds of the hyper-EPP.展开更多
Mg-based alloys are potential candidate materials for a fabrication of lightweight boron carbide based composites through a reactive melt infiltration approach. In this paper, the effect of a mechanical purification o...Mg-based alloys are potential candidate materials for a fabrication of lightweight boron carbide based composites through a reactive melt infiltration approach. In this paper, the effect of a mechanical purification of molten AZ91 alloy’s surface on its wettability with polycrystalline B_(4)C is experimentally evaluated for the first time. For this purpose, sessile drop experiments were performed under the same operating conditions(700℃/5 min;Ar atmosphere), by using both the classical contact heating(CH) and the improved capillary purification(CP) procedure. It was found that the evolution of contact angle values was strongly influenced by the applied procedure. In particular, by using the classical CH procedure, the presence of a native oxide layer on the metal surface hinders the observations of melting process, resulting in a misleading conclusion that the system is non-wettable. Contrarily, during the wetting test performed by applying the CP procedure, the surface oxide layer was mechanically removed by squeezing the molten AZ91 alloy through a capillary. Accordingly, the oxide-free AZ91 drop with a regular and spherical shape was successfully obtained and dispensed on the B_(4)C substrate. A reliable contact angle value of θ =83° was measured at the AZ91/B_(4)C triple line at 700 ℃, which in turn proves that B_(4)C is wetted by the liquid AZ91 alloy. In contradiction to the literature, these good wetting conditions were assisted by a non-reactive wetting mechanism occurring at the AZ91/B_(4)C interface. To succeed in the fabrication of AZ91/B_(4)C composites by liquid metal infiltration, such experimental observations make it reasonable to expect a spontaneous infiltration process exclusively driven by capillarity, which in turn increases the efficiency of the process by the absence of reaction products that could be a potentially detrimental factor.展开更多
Serine/arginine-rich splicing factor 1(SRSF1), as a prototype member of the highly conserved serine/arginine family of RNA binding proteins, plays an important role in mRNA alternative splicing, stabilization, nuclear...Serine/arginine-rich splicing factor 1(SRSF1), as a prototype member of the highly conserved serine/arginine family of RNA binding proteins, plays an important role in mRNA alternative splicing, stabilization, nuclear export, and translation. Here, the expression system was established to purify full-length human SRSF1 from Escherichia coli(E. coli). The SRSF1 coding sequence was amplified by polymerase chain reaction(PCR) and inserted into the pET-28 a-ppSUMO vector with His-tag to construct a recombinant plasmid His-SUMO-SRSF1. Then the plasmid was transformed into BL21(DE3) competent cells for expression. After purification by affinity chromatography and cleavage of His-SUMO moiety, a highly purified SRSF1 with a molecular weight of around 28 kg/mol was obtained. The protein was analyzed by sizing chromatography and it was found that SRSF1 would form a polymer structure in the solution. According to Expasy bioinformatics analysis, SRSF1 is extremely unstable. Purification of full-length SRSF1 protein provides an opportunity to study mRNA splicing in vitro.展开更多
Calmodulin from Homo sapiens is an α-helical calcium-binding protein that expresses to high levels in E. coli. When the N-terminus of a calmodulin variant is bound to Ca2+, it undergoes a conformational change, expos...Calmodulin from Homo sapiens is an α-helical calcium-binding protein that expresses to high levels in E. coli. When the N-terminus of a calmodulin variant is bound to Ca2+, it undergoes a conformational change, exposing hydrophobic pockets. This property can be utilized for purification purposes, as these pockets bind to phenyl sepharose resin with high affinity. Washing with EDTA chelates the Ca2+ ions from the protein, inducing a conformational change back to the more folded state and eluting the protein from the column. We describe herein the use of a protein expression and purification technique using the calmodulin variant and a short linker for proteolytic cleavage by the mutant NIa-Pro tobacco etch virus protease. We have shown this approach to be useful in obtaining purified quantities of various small proteins that could not be expressed using other methods, including high enough concentrations of a designed WW domain protein for NMR structural analysis. We have also obtained promising results on the usefulness of this procedure to express and purify zinc finger proteins without the addition of zinc ions or other cofactors.展开更多
At present, it is usually used the method that elevating the height of embankment to design passages of freeway to avoid transverse traffic disturbance in plain areas of China. But when design low embankment, it is di...At present, it is usually used the method that elevating the height of embankment to design passages of freeway to avoid transverse traffic disturbance in plain areas of China. But when design low embankment, it is difficulty for drainage or setting passages and culverts. Hengshui to Darning freeway in Hebei provence （Heng-Da freeway） is one section of Dalian to Guangzhnu freeway. This paper analyzes the characteristic of climate, geology, hydrology and low water table, good permeability stratum soil and its little and mass rain fall and deep drinking water well in the plain areas of North China when design low embankment of Heng-Da freeway, puts forward the drainage methods of water collecting filtration system to solve the drainage problem of passages and culverts, aiming at the type and the measures of the pollution on the surface, the channel collection purification infiltration system of highway uses the single composite technology of aerobic and oxygen to digest - precipitation-absorption of artificial materials percolation of soil to deal with the pollution of the rain on the road （the composite technology use the vermiculite as the material of infiltration）, which be controlled in the drinking range of the water standard. It not only efficiently solved the problem of water collected in the channel, but also supplied the groundwater. It has reached the environment-friendly road construction purpose of the sustainable development of highway construction, ＂environment friendly＂ and ＂resource conservation＂.展开更多
The large flow oil wash down purification equipment for steamturbine oil system is developed and produced by bothShandong Electric Power Test and Research Institute and ZouxianThermal Power Plant.With this equipment,t...The large flow oil wash down purification equipment for steamturbine oil system is developed and produced by bothShandong Electric Power Test and Research Institute and ZouxianThermal Power Plant.With this equipment,the turbine oil system展开更多
基金Supported by Natural Science Foundation of Hunan Province,China,No.2022JJ30842 and No.2024JJ6560Clinical Medical Research Center for Viral Hepatitis of Hunan Province,No.2023SK4009Beijing iGandan Foundation,No.RGGJJ-2021-017 and No.iGandanF-1082022-RGG023.
文摘BACKGROUND We have innovatively amalgamated membrane blood purification and centrifugal blood cell separation technologies to address the limitations of current artificial liver support(ALS)models,and develop a versatile plasma purification system(VPPS)through centrifugal plasma separation.AIM To investigate the influence of VPPS on long-term rehospitalization and mortality rates among patients with acute-on-chronic liver failure(ACLF).METHODS This real-world,prospective study recruited inpatients diagnosed with ACLF from the Second Xiangya Hospital of Central South University between October 2021 and March 2024.Patients were categorized into the VPPS and non-VPPS groups based on the distinct ALS models administered to them.Self-administered questionnaires,clinical records,and self-reported data served as the primary methods for data collection.The laboratory results were evaluated at six distinct time points.All patients were subjected to follow-up assessments for>12 months.Kaplan-Meier survival analyses and Cox proportional hazards models were used to evaluate the risks of hospitalization and mortality during the follow-up period.RESULTS A cohort of 502 patients diagnosed with ACLF was recruited,with 260 assigned to the VPPS group.On comparing baseline characteristics,the VPPS group exhibited a significantly shorter length of stay,higher incidence of spontaneous peritonitis and pulmonary aspergillosis compared to the non-VPPS group(P<0.05).Agehazard ratio(HR=1.142,95%CI:1.01-1.23,P=0.018),peritonitis(HR=2.825,95%CI:1.07-6.382,P=0.026),albumin(HR=0.67,95%CI:0.46-0.942,P=0.023),total bilirubin(HR=1.26,95%CI:1.01-3.25,P=0.021),international normalized ratio(HR=1.97,95%CI:1.21-2.908,P=0.014),and VPPS/non-VPPS(HR=3.24,95%CI:2.152-4.76,P<0.001)were identified as significant independent predictors of mortality in both univariate and multivariate analyses throughout the follow-up period.Kaplan-Meier survival analyses demonstrated significantly higher rehospitalization and mortality rates in the non-VPPS group compared to the VPPS group during follow-up of≥2 years(log-rank test,P<0.001).CONCLUSION These findings suggest that VPPS is safe and has a positive influence on prognostic outcomes in patients with ACLF.
文摘[ Objective ] The aim was to study the influence of filamentous algae on the process of water ecological purification. [ Method ] The occurrence mechanism of filamentous algae and its ecological system were summarized and analyzed. Considering the ecological purification in north- ern Jiangsu, the occurrence and prevention of filamentous algae in water, the method to prevent filamentous algae in polluted water was discussed. [ Result] The results showed that by measures of improving planting density, regular harvesting, and water flow state control before the filamentous algae blooming period, together with improving local pH value, light interference and ecological control during the blooming period, can effectively control the filamentous algae blooming. [ Condusion] The study of the happening mechanism of filamentous algae provided theoretical references and the technical basis in the work of filamentous algae prevention and control.
基金financially supported by the National Key R&D Program of China(Grant Nos.2016YFA0202704 and 2019YFA0706900)the Beijing Municipal Natural Science Foundation(Grant No.2212052)+1 种基金the China Postdoctoral Science Foundation(Grant No.2019T120390)the Jiangsu Planned Projects for Postdoctoral research funds(Grant No.2018K018A)。
文摘It is of great importance to explore a creative route to improve the degradation e ciency of organic pollutants in wastewater.Herein,we construct a unique hybrid system by combining self-powered triboelectric nanogenerator(TENG)with carbon dots-TiO_(2)sheets doped three-dimensional graphene oxide photocatalyst(3 DGA@CDs-TNs),which can significantly enhance the degradation e ciency of brilliant green(BG)and direct blue 5 B(DB)owing to the powerful interaction of TENG and 3 DGA@CDs-TNs photocatalyst.The power output of TENG can be applied for wastewater purification directly,which exhibits a selfpowered electrocatalytic technology.Furthermore,the results also verify that TENG can replace conventional electric catalyst to remove pollutants e ectively from wastewater without any consumption.Subsequently,the unstable fragments and the plausible removal pathways of the two pollutants are proposed.Our work sheds light on the development of e cient and sustainable TENG/photocatalyst system,opening up new opportunities and possibilities for comprehensive utilization of random energy.
文摘A garbage incineration power plant is proposed to be built somewhere. Due to local environmental capacity and local policy,the demand on the flue gas emission indicators of the project is high,but the conventional purification process of flue gas produced during waste incineration（ SNCR denitrification ＋dry/semi-dry deacidification ＋ adsorption of dioxins and heavy metals by activated carbon ＋ bag dusting） has been unable to meet this requirement,and the newly added wet deacidification and SCR denitrification processes can meet this requirement. The flue gas purification process was optimized,and two feasible schemes were compared to choose the better one.
基金supported by the Natural Science Foundation of Guangdong Province(2025A1515012561 and 2024A1515013152)National Natural Science Foundation of China(W2433071 and 31972367)Provincial Rural Revitalization Strategy Special Project of Guangdong in 2024(2024-NPY-00-030).
文摘Pitaya is an important perennial herbaceous fruit tree.The color of fruit determines pitaya nutritive(and attractive)value,which is considered as an important objective in breeding improvement.In this study,we reported the first telomere-to-telomere(T2T)gap-free genome of“Shuangse No.1”pitaya(Hylocereus polyrhizus;red peel).Two high-quality genomes for“Dahong”(H.polyrhizus;red peel)and“Honghuaqinglong”(H.stenopterus;stay-green)were further assembled,aiming to explore the genetic diversity of pitaya genomes.In further analysis,we noticed a high proportion of viral contamination in pitaya tissues,which hindered the efficient utilization of transcriptomic data.To address this issue,we analyzed 111 pitaya transcriptome data from different geographic regions to characterize and separate viral components.Then we developed an efficient,novel,and universal transcript purification system for pitaya transcriptomes by applying it to 27 samples from different tissues and species,thereby enhancing the utility for transcriptomic and broader biological research.Combining the purified transcriptomic data with comparative genomic analyses,we identified HuERF72,a transcription factor(TF)that potentially regulates chlorophyll degradation in pitaya.Interaction assays and plant transformation elucidated that HuERF72 acts as a repressive TF by directly binding to the promoter of HuSGR1,a key structural gene in the chlorophyll degradation pathway.This study provides high-quality genomic resources and novel methodologies for molecular investigations in pitaya.Additionally,the proposed regulatory network advances our understanding of the transcriptional regulatory mechanisms underlying chlorophyll degradation,offering valuable insights into the genetic improvement of pitaya.
基金supported by the National Key Research and Development Program of China(2021YFC2301805)。
文摘The artificial liver blood purification system(ALBPS),referred to as the artificial liver system,has been extensively used for treatment of patients with liver failure or other organ failures,and its efficacy has been well-documented.To further standardize the clinical application of ALBPS,the Liver Failure and Artificial Liver Group,Chinese Society of Infectious Diseases,Chinese Medical Association and other institutions invited experts to update the 2016 version of ALBPS guidelines and develop the Guidelines on Application of Artificial Liver Blood Purification System(version 2023).In line with the latest international research advancements,this updated version compre-hensively elaborates on various aspects of ALBPS,including treatment mode selection,anticoagulation program implementation,nursing and operational procedures,indications and relative contraindications,common complications and their management,as well as management regulations of the system.These guidelines will provide guidance for the effective application of ALBPS for treatment of critically ill patients.
基金Supported by Special Fund for the Construction of Modern Agricultural Industry Technology System(CARS-46)Special Fund for Basic Research and Operating Expenses of Central-level Research Institutes(2007JBFA03)~~
文摘The establishment of water circulation aquaculture system realized the hi- erarchical use of nitrogen, phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource. However, no research has been reported on the detailed calculation of the relationship between the area of aquaculture pond and purification pond. In this study, referring to the absorption ability of aquatic plants to pollutants in aquaculture wastewater and pollutant generation and discharge coefficient in aquaculture pond, based on the general rules of water quality management in freshwater aquaculture system, a calculation mode was es- tablished to investigate the relationship between the area of aquaculture pond and purification pond in freshwater recirculation aquaculture system, which was feasible to explain related cases and would provide theoretical basis to reduce the economic costs in the construction of water circulation aquaculture system and realize the bal- ance between the ecological benefits and the economic benefits.
基金supported by the National Nature Science Foundation of China (No.51878442).
文摘High-concentration oil mists can cause serious health problems to workers,which are generally mitigated by ventilation and purification systems.However,the coupling relationship between these systems is not clear.In this study,the effects of purifier outlet direction,purification air volume,installation height,and purification efficiency on indoor oil mist distribution were investigated by numerical simulation using an actual machining factory.The mitigation of oil mist in various combinations of ventilation and purification systems was also discussed.The results show that the outlet direction of the purifier has a great influence on the distribution of oil mist in the factory,and the maximum difference of oil mist concentration in the breathing zone under different orientations is 17%.The best purifier outlet direction is vertically upward.When the purifier outlet direction is upward,a larger purification air volume is beneficial for contaminant removal,and a lower purifier exhaust installation height is helpful for oil mist discharge from the bottom of the factory and reducing the concentration of oil mist in the breathing area.The oil mist concentration of purifier exhaust increases from 0 to 2 mg/m^(3) and the oil mist concentration in the breathing zone increases by 67%.The combined system of purification system with the roof exhaust system and displacement ventilation system has the optimal pollution removal efficiency and the lowest concentration of oil mist in the breathing zone compared to other systems.The research results can provide a reference for the design,installation,and operation of ventilation and purification systems in machining factories.
基金Key Project of Science and Technology Department of Wuhan(zz2011-12)Key Project of the Educational Bureau of Wuhan(sz2011-13-10)+1 种基金Project of Science and Technology Department of Hubei(2010CDB04801)The State Key Laboratory program of Viral Genetic Engineering(2010KF10)
文摘Enterovirus type 71（EV71） causes severe hand-foot-and-mouth disease （HFMD） resulting in hundreds of deaths of children every year; However, currently, there is no effective treatment for EV71. In this study, the EV71 poly-protein （EV71-P1 protein） gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GSll5. The EV71 P1 protein with a molecular weight of 100 kD was produced and secreted into the medium. The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%. The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits. We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.
基金Project(2005A20303002) supported by the Science and Technology Plan Item of Guangdong Province, ChinaProject(2006J1-C0251) supported by the Science and Technology Bureau Foundation of Guangzhou, China
文摘A system of polar ordered resins was established for purification of chlorogenic acid in Flos Lonicerae. It was composed of three reversed phase resins, AB-8, DM-130 and NKA-9, representative for their gradually increased polarity and selectivity. A method of RP-HPLC was used for determination of chlorogenic acid. And the performance of adsorption and desorption for chlorogenic acid with the system of polar ordered resins was studied. Furthermore, the effects of concentration, pH and flow rate of the adsorbate on adsorption ability were researched. It is indicated that the optimum parameters for chlorogenic acid are as follows: pH 3.5 with a flow rate of 2.5 BVh, the concentration of extract solution at 0.50, 0.40, 0.30 gL respectively for the adsorptive operation twice, and 6.93, 8.66, 10.39 molL ethanol used as gradient eluants. The purity of resulted product of chlorogenic acid arrives 70.20% with yield of 89.79%. With simple procedures, low costs and high purity product, the method of system of polar ordered resins followed by sequential reversed phase separations can be used to refine the chlorogenic acid in the extraction of Flos Lonicerae.
基金Project supported in part by the National Natural Science Foundation of China(Grant No.61901420)the Shanxi Provincial Science Foundation for Youths(Grant No.201901D211235)the Scientific and Technological Innovation Program of Higher Education Institutions of Shanxi Province,China(Grant No.2019L0507)。
文摘We present a self-error-rejecting multipartite entanglement purification protocol(MEPP)for N-electron-spin entangled states,resorting to the single-side cavity-spin-coupling system.Our MEPP has a high efficiency containing two steps.One is to obtain high-fidelity N-electron-spin entangled systems with error-heralded parity-check devices(PCDs)in the same parity-mode outcome of three electron-spin pairs,as well as M-electron-spin entangled subsystems(2≤M<N)in the different parity-mode outcomes of those.The other is to regain the N-electron-spin entangled systems from Melectron-spin entangled states utilizing entanglement link.Moreover,the quantum circuits of PCDs make our MEPP works faithfully,due to the practical photon-scattering deviations from the finite side leakage of the microcavity,and the limited coupling between a quantum dot and a cavity mode,converted into a failed detection in a heralded way.
文摘The Curaua. （Ananaserectifolius LB Smith） is a species belonging to the family Bromeliaceae. Occurs in the states of Para, Amazonas, Amapa, Acre, Mato Grosso and Goia s, has two varieties, purple and white, and its fibers are used in automotive and textile industries due to its strength, softness and lightweight. Currently, only the fiber is used in industry, the rest is considered waste However, this residue contains compounds with important properties to be discovered and studied. An enzyme complex found in this residue is bromelain, a group of proteolysis＇ enzymes with application in several areas such as in the food, pharmaceutical and cosmetics industries. Today, its use is aiming the pharmaceutical industry, the production of ointments, gels, creams and lotions because they offer a wide range of therapeutic efficacies： antiedemas, anti-inflammatory, antithrombotic and fibrinolytic activities. This work studied the enzyme purification and recovery presented in the leaves of Curaua by means of an ATPS （aqueous two-phase system） PEG 4000/potassium phosphate. The protein content was measured by Bradford reagent and the enzymatic activity was measured by the Biuret reagent. Batch assays were performed aiming the enzyme extraction and recuperation, using the partition coefficient as indicator. It was used pH 7.0, 8.0 and 9.0, varying the proportional composition between the polymeric and the saline phases （tie-lines）. The white variety of Curaua. was used. The best purification factor was 4.53 and about 113.04 μg/mL of total protein measured by Bradford.
文摘Objective:To study the shock index of patients with sepsis after continuous blood purification (CBP) treatment and its relationship with systemic inflammatory response syndrome and immune response.Methods:A total of 88 patients with sepsis who underwent continuous blood purification treatment in our hospital between June 2012 and May 2016 were chosen as research subjects, shock index (SI) was compared before and after the treatment, and according to the level of SI after treatment, all patients were divided into shock group (n=27) with SI>0.5 points and no shock group (n=61) with SI≤0.5 points. Serum contents of inflammatory mediators, Th1/Th2 cellular immunity indexes, immunoglobulin and complement were compared between two groups of patients after treatment.Results: The level of SI in patients with sepsis was significantly lower than that before treatment. Serum contents of inflammatory mediators PCT, CRP and HMGB1 in no shock group were lower than those in shock group, contents of Th1 cytokines IL-2 and IFN-γ were higher than those in shock group, contents of Th2 cytokines IL-10 and IL-13 were lower than those in shock group, and contents of IgG, IgM, IgA, C3 and C4 were higher than those in shock group.Conclusion: The level of SI decreases in the patients with sepsis after CBP treatment, and SI level is directly correlated with the systemic inflammatory response syndrome and immune response levels.
基金Project supported by the National Natural Science Foundation of China(Grant No.61901420 and 11604226)the Shanxi Province Science Foundation for Youths(Grant No.201901D211235)+1 种基金the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(Grant No.2019L0507)the Program of Beijing Municipal Commission of Education of China(Grant Nos.CIT&TCD201904080 and KM201810028005)。
文摘We present a faithful and efficient hyperentanglement purification protocol(hyper-EPP)for nonlocal two-photon systems in spatial-polarization-time-bin hyperentangled Bell states.As the single-photon detectors can detect and herald the undesirable properties caused by side leakage and finite coupling strength,the parity-check gates and swap gates of our hyper-EPP in the spatial,polarization and time-bin mode degrees of freedom(DoFs)work faithfully.The qubit-flip errors in photon systems in three DoFs can be corrected effectively with the faithful parity-check gates and the photon pairs can be reused to distill high-fidelity ones by introducing the faithful swap gates,which greatly increases the efficiency of our hyper-EPP.Further,the maximal hyperentanglement can be obtained in principle by operating multiple rounds of the hyper-EPP.
基金the financial support given by the National Science Centre (NCN) in Poland,under the project MINIATURA 2, No. 2018/02/X/ST8/03044 in2019–2020。
文摘Mg-based alloys are potential candidate materials for a fabrication of lightweight boron carbide based composites through a reactive melt infiltration approach. In this paper, the effect of a mechanical purification of molten AZ91 alloy’s surface on its wettability with polycrystalline B_(4)C is experimentally evaluated for the first time. For this purpose, sessile drop experiments were performed under the same operating conditions(700℃/5 min;Ar atmosphere), by using both the classical contact heating(CH) and the improved capillary purification(CP) procedure. It was found that the evolution of contact angle values was strongly influenced by the applied procedure. In particular, by using the classical CH procedure, the presence of a native oxide layer on the metal surface hinders the observations of melting process, resulting in a misleading conclusion that the system is non-wettable. Contrarily, during the wetting test performed by applying the CP procedure, the surface oxide layer was mechanically removed by squeezing the molten AZ91 alloy through a capillary. Accordingly, the oxide-free AZ91 drop with a regular and spherical shape was successfully obtained and dispensed on the B_(4)C substrate. A reliable contact angle value of θ =83° was measured at the AZ91/B_(4)C triple line at 700 ℃, which in turn proves that B_(4)C is wetted by the liquid AZ91 alloy. In contradiction to the literature, these good wetting conditions were assisted by a non-reactive wetting mechanism occurring at the AZ91/B_(4)C interface. To succeed in the fabrication of AZ91/B_(4)C composites by liquid metal infiltration, such experimental observations make it reasonable to expect a spontaneous infiltration process exclusively driven by capillarity, which in turn increases the efficiency of the process by the absence of reaction products that could be a potentially detrimental factor.
基金Shanghai Science and Technology Commission’s “Belt and Road Initiative International Cooperation Project”,China (No.19410741800)。
文摘Serine/arginine-rich splicing factor 1(SRSF1), as a prototype member of the highly conserved serine/arginine family of RNA binding proteins, plays an important role in mRNA alternative splicing, stabilization, nuclear export, and translation. Here, the expression system was established to purify full-length human SRSF1 from Escherichia coli(E. coli). The SRSF1 coding sequence was amplified by polymerase chain reaction(PCR) and inserted into the pET-28 a-ppSUMO vector with His-tag to construct a recombinant plasmid His-SUMO-SRSF1. Then the plasmid was transformed into BL21(DE3) competent cells for expression. After purification by affinity chromatography and cleavage of His-SUMO moiety, a highly purified SRSF1 with a molecular weight of around 28 kg/mol was obtained. The protein was analyzed by sizing chromatography and it was found that SRSF1 would form a polymer structure in the solution. According to Expasy bioinformatics analysis, SRSF1 is extremely unstable. Purification of full-length SRSF1 protein provides an opportunity to study mRNA splicing in vitro.
文摘Calmodulin from Homo sapiens is an α-helical calcium-binding protein that expresses to high levels in E. coli. When the N-terminus of a calmodulin variant is bound to Ca2+, it undergoes a conformational change, exposing hydrophobic pockets. This property can be utilized for purification purposes, as these pockets bind to phenyl sepharose resin with high affinity. Washing with EDTA chelates the Ca2+ ions from the protein, inducing a conformational change back to the more folded state and eluting the protein from the column. We describe herein the use of a protein expression and purification technique using the calmodulin variant and a short linker for proteolytic cleavage by the mutant NIa-Pro tobacco etch virus protease. We have shown this approach to be useful in obtaining purified quantities of various small proteins that could not be expressed using other methods, including high enough concentrations of a designed WW domain protein for NMR structural analysis. We have also obtained promising results on the usefulness of this procedure to express and purify zinc finger proteins without the addition of zinc ions or other cofactors.
文摘At present, it is usually used the method that elevating the height of embankment to design passages of freeway to avoid transverse traffic disturbance in plain areas of China. But when design low embankment, it is difficulty for drainage or setting passages and culverts. Hengshui to Darning freeway in Hebei provence （Heng-Da freeway） is one section of Dalian to Guangzhnu freeway. This paper analyzes the characteristic of climate, geology, hydrology and low water table, good permeability stratum soil and its little and mass rain fall and deep drinking water well in the plain areas of North China when design low embankment of Heng-Da freeway, puts forward the drainage methods of water collecting filtration system to solve the drainage problem of passages and culverts, aiming at the type and the measures of the pollution on the surface, the channel collection purification infiltration system of highway uses the single composite technology of aerobic and oxygen to digest - precipitation-absorption of artificial materials percolation of soil to deal with the pollution of the rain on the road （the composite technology use the vermiculite as the material of infiltration）, which be controlled in the drinking range of the water standard. It not only efficiently solved the problem of water collected in the channel, but also supplied the groundwater. It has reached the environment-friendly road construction purpose of the sustainable development of highway construction, ＂environment friendly＂ and ＂resource conservation＂.
文摘The large flow oil wash down purification equipment for steamturbine oil system is developed and produced by bothShandong Electric Power Test and Research Institute and ZouxianThermal Power Plant.With this equipment,the turbine oil system
