BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
围绕通信不间断电源(Uninterruptible Power Supply,UPS)系统中多电平脉冲宽度调制(Pulse Width Modulation,PWM)逆变拓扑的谐波控制展开研究,深入分析多电平逆变结构在提升输出波形质量和降低谐波失真方面的技术优势。结合通信电源对...围绕通信不间断电源(Uninterruptible Power Supply,UPS)系统中多电平脉冲宽度调制(Pulse Width Modulation,PWM)逆变拓扑的谐波控制展开研究,深入分析多电平逆变结构在提升输出波形质量和降低谐波失真方面的技术优势。结合通信电源对高可靠性和低干扰的实际需求,构建基于PWM优化与滤波器参数设计的复合型谐波控制策略。通过建模仿真实验对不同调制方式与滤波结构进行对比分析,结果表明所提方法在降低总谐波失真、提升系统稳定性与电能质量方面具有明显优势,可为通信UPS系统的结构优化和工程应用提供理论支撑与实践参考。展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
文摘围绕通信不间断电源(Uninterruptible Power Supply,UPS)系统中多电平脉冲宽度调制(Pulse Width Modulation,PWM)逆变拓扑的谐波控制展开研究,深入分析多电平逆变结构在提升输出波形质量和降低谐波失真方面的技术优势。结合通信电源对高可靠性和低干扰的实际需求,构建基于PWM优化与滤波器参数设计的复合型谐波控制策略。通过建模仿真实验对不同调制方式与滤波结构进行对比分析,结果表明所提方法在降低总谐波失真、提升系统稳定性与电能质量方面具有明显优势,可为通信UPS系统的结构优化和工程应用提供理论支撑与实践参考。
