An effective method for isolation of total RNA from pulps of mainly deciduous fruit trees was discussed in this paper. Here we shown that total RNA was successfully isolated from pulps of apple, peach and strawberry, ...An effective method for isolation of total RNA from pulps of mainly deciduous fruit trees was discussed in this paper. Here we shown that total RNA was successfully isolated from pulps of apple, peach and strawberry, but not grape berry via the effective CTAB method modified by ZENG, et al. However, total RNA was isolated from grape berry according to ZENG＇s protocols with a modification including 16% of β-Mercatoethanol and 3% of PVP in extraction buffer, 1/3 （V/V） of 5mol/L potassium acetate （KAC PH4.8） in supernatant. The detection of the isolated total RNA above using ultraviolet spectrophoto-metry, electrophoresis, reverse transcription and RT-PCR shown that the CTAB and its modifications is a preferred method for isolation of high-quality total RNA from fruit pulps.展开更多
Amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Saccharomyces diastaticus 2047 isolated from cassava waste showed amylase and glucoamylase production, using starch ...Amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Saccharomyces diastaticus 2047 isolated from cassava waste showed amylase and glucoamylase production, using starch medium, and the highest rate was obtained in the initial growth phase, after incubation for 24 h at pH 5.5. Maximum amylase and glucoamylase activities （483.62 U mg^-1 protein and 290.85 U mg^-1 protein） were obtained at pH 5.5. The isolated enzymes exhibited thermostable properties as indicated by retention of 100% of residual activity at 55 ℃ for 45 min with total inhibition at 100 ℃. Extracellular enzyme from S diastaticus 2047 was partially purified by fractionated precipitation with ammonium sulphate. After 40% saturation produced 2,197.00 and 1,192.83 U/mg protein, and yield was 40% with purification 4.54 and 4.1 fold, respectively. This study presents feasibility on ethanol production from cassava pulps pretreated with diluted sulfuric acid by simultaneous saccharification and fermentation （SSF） with S. diastaticus 2047. The results indicated that the culture was able to produce ethanol with high yield without amylolytic enzyme adding by using cassava pulps pretreated with distilled water at 135 ℃ under pressure of 15 lb/inch^2 to produce ethanol yield as high as the cassava pulps pretreated with diluted sulfuric acid under the same condition. This suggests that S diastaticus with enzyme produced has potential for industrial applications.展开更多
Carbon dots(CDs)have been attracted to nanocarbon materials for metal ion sensing,biological activity,and plant phytotoxicity due to their excellent photophysical properties,such as low cytotoxicity,high quantum yield...Carbon dots(CDs)have been attracted to nanocarbon materials for metal ion sensing,biological activity,and plant phytotoxicity due to their excellent photophysical properties,such as low cytotoxicity,high quantum yield,tunable fluorescence emission,and biocompatibility.Cassava pulp,which consists mainly of starch,has been identified as a low-cost biomass waste from the cassava starch industry.Therefore,this research developed CDs and nitrogen-doped CDs(NCDs)from cassava pulp using a one-step hydrothermal process in deionized water at 200℃.The effects of the synthesis conditions,including reaction time(6-24 h)and the nitrogen doping derivatives,were also investigated.CDs and ethylenediamine doped-NCDs exhibited tunable fluorescence emission,strong quantum yield,high photostability,and tolerance to photobleaching.Furthermore,the potential applications of CDs-12 h were demonstrated such as fluorescent sensors for metal ion sensing,antioxidant activity,and mercury detoxification in plants.Fluorescence quenching of the CDs-12 h via both static and dynamic quenching mechanisms was observed in the presence of several metal ions such as Hg^(2+),Cu^(2+),and Fe^(3+)with the detection limit in micromolar levels and further applied to real water samples with good recovery and acceptable relative standard derivation.The paper test strip coated with CDs-12 h could also detect these metal ions under UV light.CDs and NCDs-EDA also showed potential DPPH radical scavenging activity and alleviated mercury toxicity in the Chinese cabbage seedlings with the incubation of CDs-12 h and NCDs-EDA-12 h(30 mg/L).展开更多
Pulpotomy,which belongs to vital pulp therapy,has become a strategy for managing pulpitis in recent decades.This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing l...Pulpotomy,which belongs to vital pulp therapy,has become a strategy for managing pulpitis in recent decades.This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes.Pulpotomy is categorized into partial pulpotomy(PP),the removal of a partial segment of the coronal pulp tissue,and full pulpotomy(FP),the removal of whole coronal pulp,which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth.Procedural decisions for the amount of pulp tissue removal or retention depend on the diagnostic of pulp vitality,the overall treatment plan,the patient’s general health status,and pulp inflammation reassessment during operation.This statement represents the consensus of an expert committee convened by the Society of Cariology and Endodontics,Chinese Stomatological Association.It addresses the current evidence to support the application of pulpotomy as a potential alternative to root canal treatment(RCT)on mature permanent teeth with pulpitis from a biological basis,the development of capping biomaterial,and the diagnostic considerations to evidence-based medicine.This expert statement intends to provide a clinical protocol of pulpotomy,which facilitates practitioners in choosing the optimal procedure and increasing their confidence in this rapidly evolving field.展开更多
Stem cells play a crucial role in maintaining tissue regenerative capacity and homeostasis.However,mechanisms associated with stem cell senescence require further investigation.In this study,we conducted a proteomic a...Stem cells play a crucial role in maintaining tissue regenerative capacity and homeostasis.However,mechanisms associated with stem cell senescence require further investigation.In this study,we conducted a proteomic analysis of human dental pulp stem cells(HDPSCs)obtained from individuals of various ages.Our findings showed that the expression of NUP62 was decreased in aged HDPSCs.We discovered that NUP62 alleviated senescence-associated phenotypes and enhanced differentiation potential both in vitro and in vivo.Conversely,the knocking down of NUP62 expression aggravated the senescence-associated phenotypes and impaired the proliferation and migration capacity of HDPSCs.Through RNA-sequence and decoding the epigenomic landscapes remodeled induced by NUP62 overexpression,we found that NUP62 helps alleviate senescence in HDPSCs by enhancing the nuclear transport of the transcription factor E2F1.This,in turn,stimulates the transcription of the epigenetic enzyme NSD2.Finally,the overexpression of NUP62 influences the H3K36me2 and H3K36me3 modifications of anti-aging genes(HMGA1,HMGA2,and SIRT6).Our results demonstrated that NUP62 regulates the fate of HDPSCs via NSD2-dependent epigenetic reprogramming.展开更多
Pulpitis is a common infective oral disease in clinical situations.The regulatory mechanisms of immune defense in pulpitis are still being investigated.Osteomodulin(OMD)is a small leucine-rich proteoglycan family memb...Pulpitis is a common infective oral disease in clinical situations.The regulatory mechanisms of immune defense in pulpitis are still being investigated.Osteomodulin(OMD)is a small leucine-rich proteoglycan family member distributed in bones and teeth.It is a bioactive protein that promotes osteogenesis and suppresses the apoptosis of human dental pulp stem cells(hDPSCs).In this study,the role of OMD in pulpitis and the OMD-induced regulatory mechanism were investigated.The OMD expression in normal and inflamed human pulp tissues was detected via immunofluorescence staining.Intriguingly,the OMD expression decreased in the inflammatory infiltration area of pulpitis specimens.The cellular experiments demonstrated that recombined human OMD could resist the detrimental effects of lipopolysaccharide(LPS)-induced inflammation.A conditional Omd knockout mouse model with pulpal inflammation was established.LPS-induced inflammatory impairment significantly increased in conditional Omd knockout mice,whereas OMD administration exhibited a protective effect against pulpitis.Mechanistically,the transcriptome alterations of OMD overexpression showed significant enrichment in the nuclear factor-κB(NF-κB)signaling pathway.Interleukin-1 receptor 1(IL1R1),a vital membrane receptor activating the NF-κB pathway,was significantly downregulated in OMD-overexpressing hDPSCs.Additionally,the interaction between OMD and IL1R1 was verified using co-immunoprecipitation and molecular docking.In vivo,excessive pulpal inflammation in Omd-deficient mice was rescued using an IL1R antagonist.Overall,OMD played a protective role in the inflammatory response via the IL1R1/NF-κB signaling pathway.OMD may optimize the immunomodulatory functions of hDPSCs and can be used for regenerative endodontics.展开更多
Dental pulp-dentin complex defects remain a major unresolved problem in oral medicines.Clinical therapeutic methods including root canal therapy and vital pulp therapy are both considered as conservative strategies,wh...Dental pulp-dentin complex defects remain a major unresolved problem in oral medicines.Clinical therapeutic methods including root canal therapy and vital pulp therapy are both considered as conservative strategies,which are incapable of repairing the pulpdentin complex defects.Although biomaterial-based strategies show remarkable progress in antibacterial,anti-inflammatory,and pulp regeneration,the important modulatory effects of nerves within pulp cavity have been greatly overlooked,making it challenging to achieve functional pulp-dentin complex regeneration.In this study,we propose an injectable bioceramicscontaining composite hydrogel in combination of Li-Ca-Si(LCS)bioceramics and gelatin methacrylate matrix with photocrosslinking properties.Due to the sustained release of bioactive Li,Ca and Si ions from LCS,the composite hydrogels possess multiple functions of promoting the neurogenic differentiation of Schwann cells,odontogenic differentiation of dental pulp stem cells,and neurogenesis-odontogenesis couples in vitro.In addition,the in vivo results showed that LCS-containing composite hydrogel can significantly promote the pulp-dentin complex repair.More importantly,LCS bioceramics-containing composite hydrogel can induce the growth of nerve fibers,leading to the re-innervation of pulp tissues.Taken together,the study suggests that LCS bioceramics can induce the innervation of pulp-dentin complex repair,offering a referable strategy of designing multifunctional filling materials for functional periodontal tissue regeneration.展开更多
On January 3:Hubei Xianhe New Materials Co.,Ltd.Phase I project was officially put into production,with a total investment of 10 billion CNY and a planned pulp and paper production capacity of 2.5 million tons per yea...On January 3:Hubei Xianhe New Materials Co.,Ltd.Phase I project was officially put into production,with a total investment of 10 billion CNY and a planned pulp and paper production capacity of 2.5 million tons per year.On January 10:The pulp and paper project of Huatai Group is accelerating construction.With a total investment of 10.2 billion CNY,after completion,Huatai's newsprint production capacity will be reduced from 1.4 million tons per year to 400000 tons per year,while cultural paper capacity will increase from 100000 tons per yeart to 1.6 million tons per year,achieving annual sales revenue of 19 billion CNY and profits and taxes of 3.2 billion CNY.展开更多
Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with l...Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with less than 20%mass loss rate by bio-pulping synergistic treatment of straw fibers with deep eutectic solvent(DES)and Streptomyces rochei(S.rochei).Results illustrated that at 3%S.rochei concentration with 7-day fermentation,both cellulose and hemicellulose enzyme activities of the treated rice straw fiber reached peak values with a fiber mass loss rate of 17.01%.Microstructural morphology revealed that S.rochei colonization initiated on straw surfaces and progressively penetrated internal structures,resulting in surface loosening and distinct disruption of cell wall tissues within vascular bundles in transverse sections.The treated rice straw strip indicated a maximum tensile strength of 46.22 MPa for(Bacteria)BA 3%at day 7,attributed to optimized synergistic effects of microfibril angle(MFA)and cellulose/hemicellulose relative content ratio.The modified straw pulp film exhibited significant enhancement in the tensile index(44.9%increase),burst index(10.3%increase),and tear index(60%increase)compared to untreated groups.This work demonstrated the important role ofDES and S.rochei bio-pulping synergistic treatment in improving rice straw pulp performance,suggesting an eco-friendly,novel,and efficient biomass pretreatment technology for potential application prospects in sustainable agricultural mulching materials.展开更多
Circulose,a global leader in recycled pulp production,hasreached a strategic partnership agreement with Tangshan SanyouChemical Fiber,a front-runner in the cellulose fiber industry.Thiscollaboration marks a significan...Circulose,a global leader in recycled pulp production,hasreached a strategic partnership agreement with Tangshan SanyouChemical Fiber,a front-runner in the cellulose fiber industry.Thiscollaboration marks a significant milestone in the commercializa-tion and scaling of CIRCULOSER,whle also propelling TangshanSanyou's brand toward greater international recognition.展开更多
Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on t...Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes.展开更多
BACKGROUND The two-way,three-stage psychological intervention for patients with dental pulp disease offers a reference for nursing interventions in such cases.AIM To examine the effects of a three-stage psychological ...BACKGROUND The two-way,three-stage psychological intervention for patients with dental pulp disease offers a reference for nursing interventions in such cases.AIM To examine the effects of a three-stage psychological intervention on psychological resilience and health behaviors.METHODS A total of 114 patients with dental pulp disease treated between December 2022 and December 2023 were allocated into two groups according to the random lottery method,with 57 patients in each group.The control group adopted the teaching method,while the observation group used a three-stage psychological intervention combined with the teaching method.We compared psychological resilience,coping strategies,dental fear,health behavior habits,and stigma between the two groups.RESULTS The intervention group showed significantly improved scores on the psychological resilience scale(Connor-Davidson Resilience Scale)(P<0.05);positive and negative response scores also improved after the intervention(P<0.05);significant differences were observed between the observation and control groups in the Chinese version of the Stouthard Dental Fear Scale(Dental Anxiety Inventory),the Social Impact Scale,and health behavior score(P<0.05).CONCLUSION Combining a three-stage psychological intervention with the back-teaching method effectively reduces dental fear and stigma in patients with dental pulp disease.It also improves psychological resilience,coping strategies,and health behavior habits,achieving significant results.展开更多
This study examines the development of loose-fill thermal insulation materials derived from annual plant residues,such as wheat straw,water reeds,and corn stalks,processed using the chemimechanical pulping(CMP)techniq...This study examines the development of loose-fill thermal insulation materials derived from annual plant residues,such as wheat straw,water reeds,and corn stalks,processed using the chemimechanical pulping(CMP)technique.The chopped plants were soda-cooked for 30 min,varying NaOH concentration(2%–8%on a dry basis of biomass),and mechanically refined using different disc types.The CMPprocess enhances the homogeneity and stability of defibratedmaterial,yielding improved insulation properties compared to untreated chopped rawmaterials.Chemical analysis revealed that CMP increases cellulose content and reduces lignin levels,enhancing water retention and vapor diffusion properties.Settlement tests confirmed that CMP materials are more resistant to compaction under vibration,maintaining long-term performance.Additionally,the CMP enables the production of lightweight materials that require less resource consumption while achieving comparable thermal insulation performance.The investigated biobased materials offer a sustainable alternative to conventional insulation,with competing thermal conductivity values(0.041-0.046 W/mK)at the settlement-resistant bulk density level of 60 kg/m^(3).The thermal conductivity of CMP materials remains minimally affected.However,the resulting fibers demonstrate significant advantages in stability and material efficiency.This highlights its suitability for loose-fill applications to improve the sustainability of the construction.Using renewable plant residues,CMP-based insulation materials align with circular economy principles and contribute to environmental sustainability.This research underscores the potential of CMP materials to reduce greenhouse gas emissions,optimize resource use,and promote eco-friendly building practices.展开更多
From August 12 to 15,the 2025 China International Paper Technology Exhibition(hereina?t er referred to as“CIPTE”)was successfully held in Shanghai.Hosted by the China Paper Association,China Technical Association of...From August 12 to 15,the 2025 China International Paper Technology Exhibition(hereina?t er referred to as“CIPTE”)was successfully held in Shanghai.Hosted by the China Paper Association,China Technical Association of Paper Industry,and China National Pulp and Paper Research Institute Co.,Ltd.(below referred to as“CNPPRI”),and organized by China Pulp and Paper Magazines Publisher,this event received strong support and extensive attention from industry peers.展开更多
Tooth pulpitis is a prevalent oral disorder.Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies hold great significance.Ferroptosis has recently emerged as a new form of c...Tooth pulpitis is a prevalent oral disorder.Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies hold great significance.Ferroptosis has recently emerged as a new form of cell death,but the role of ferroptosis in pulpitis remains largely unknown.In our study,single-cell RNA sequencing(sc RNA-seq)was used to identify cellular heterogeneity between 3 pulpitis tissue and 3 healthy pulp tissue,and explored ferroptosis occurrence in pulpitis tissue and inflamed dental pulp cells(DPCs).In scRNA-seq,40231 cells(Pulpitis:17814;Healthy pulp:22417)were captured,and visualized into 12 distinct cell clusters.Differential y expressed ferroptosis-related genes(DE-FRGs)were almost presented in each cluster in pulpitis vs healthy pulp.ROS and Fe^(2+)levels significantly rose,and immunohistochemistry showed low expression of GPX4 and high expression of PTGS2 in pulpitis.In LPSstimulated DPCs,thymosinα1 increased the expression of GPX4 and FTL,and decreased expression of TNF-α,IL-1β,IL-6,and Fe^(2+)levels.In rat pulpitis models,both prothymosinα(PTMA,precursor of thymosinα1)gelatin sponge placed at the hole of pulp(LPS-P(gs))and PTMA injection in pulp(LPS-P(i))significantly reduced infiltration of inflammatory cells and expression of PTGS2,and increased the expression of GPX4.In RNA sequencing,the expression of DE-FRGs were reversed when thymosinα1 were added in LPS-stimulated DPCs.Collectively,single-cell atlas reveals cellular heterogeneity between pulpitis and healthy pulp,and ferroptosis occurrence in pulpitis.Thymosinα1 may reduce ferroptosis in DPCs to alleviate pulpitis and thus potentially has the ability to treat pulpitis.展开更多
The rheological behavior of low consistency thermomechanical pulp of Chinese fir harvested by intermediate thinning was analyzed. The results show that the apparent viscosity of pulp changed along with the beating deg...The rheological behavior of low consistency thermomechanical pulp of Chinese fir harvested by intermediate thinning was analyzed. The results show that the apparent viscosity of pulp changed along with the beating degree, pulp consistency and shearing velocity. With the increasing of pulp consistency, the apparent viscosity of pulp increased gradually. Beating degree of pulp had an effect on microstructure of pulp. The apparent viscosity of pulp declined as beating degree of pulp increased, and the apparent viscosity of pulp fell along with the shearing velocity increasing. Based on the results, the rheological models are set up. The models showed that the fluid types of the low consistency pulp could be described as pseudoplastics fluids （non-Newtonian fluids）.展开更多
Cationized pulp fibers （CPF） were prepared by the adsorption of a novel biodegradable cationic ester quaternary ammonium salt （31441） on bleached softwood kraft pulp fibers. The optimized conditions for the CPF pr...Cationized pulp fibers （CPF） were prepared by the adsorption of a novel biodegradable cationic ester quaternary ammonium salt （31441） on bleached softwood kraft pulp fibers. The optimized conditions for the CPF preparation were： 4% of 31441 （based on oven-dry pulp）, 80℃ and 30 rain. The CPF was characterized by FT-IR, SEM and XPS. Experimental results showed that the CPF improved the retention of precipitated calcium carbonate （PCC） filler significantly. With 0.9% CPF （based on oven-dry pulp）, the retention of PCC increased from 57.53% to 72.21%. The physical properties of paper were also slightly improved. The tensile strength and burst strength of the paper with CPF were higher than those with CPAM. CPF addition had no effect on the stock drainage.展开更多
A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated？ Can adult stem cells be...A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated？ Can adult stem cells be orchestrated to regenerate tooth structures such as the enamel, dentin, cementum and dental pulp, or even an entire tooth？ If not, what are the therapeutically viable sources of stem cells for tooth regeneration？ Do stem cells necessarily need to be taken out of the body, and manipulated ex vivo before they are transplanted for tooth regeneration？ How can regenerated teeth be economically competitive with dental implants？ Would it be possible to make regenerated teeth affordable by a large segment of the population worldwide？ This review article explores existing and visionary approaches that address some of the above-mentioned questions. Tooth regeneration represents a revolution in stomatology as a shift in the paradigm from repair to regeneration： repair is by metal or artificial materials whereas regeneration is by biological restoration. Tooth regeneration is an extension of the concepts in the broad field of regenerative medicine to restore a tissue defect to its original form and function by biological substitutes.展开更多
Given the gradual increase in the chlorite content of hematite ores, pulp properties seriously deteriorate during flotation. The traditional anion reverse flotation of hematite cannot effectively eliminate the effects...Given the gradual increase in the chlorite content of hematite ores, pulp properties seriously deteriorate during flotation. The traditional anion reverse flotation of hematite cannot effectively eliminate the effects of chlorite, leading to a significant decrease in the total Fe(TFe) grade of the concentrate. In this work, the effect of sodium alginate on the reverse flotation of hematite was systematically investigated. Flotation tests of artificially mixed ores were conducted, and the results showed that sodium alginate can significantly improve the removal rates of quartz and chlorite. The adsorption measurements, infrared spectroscopy, and contact angle tests demonstrated that sodium alginate adsorbs on the quartz surface by chelating with calcium ions, thereby weakening the steric hindrance of oleate ions and increasing the adsorption capacity of sodium oleate to ultimately improve the removal rate of quartz. Furthermore, owing to its lower density and fine particle size, chlorite is easily entrained into the foam layer. Sodium alginate dramatically increases the liquid-to-gas ratio of the foam layer by increasing pulp viscosity, thereby increasing the entrainment rate of chlorite and finally improving its removal rate. The core content of this thesis bears significance in improving the Fe grade in the reverse flotation of chlorite-containing hematite.展开更多
Stem cells are pluripotent cells, having a property of differentiating into various types of cells of human body. Several studies have developed mesenchymal stem cells(MSCs) from various human tissues,peripheral blood...Stem cells are pluripotent cells, having a property of differentiating into various types of cells of human body. Several studies have developed mesenchymal stem cells(MSCs) from various human tissues,peripheral blood and body fluids. These cells are then characterized by cellular and molecular markers to understand their specific phenotypes. Dental pulp stem cells(DPSCs) are having a MSCs phenotype and they are differentiated into neuron, cardiomyocytes, chondrocytes, osteoblasts, liver cells and β cells of islet of pancreas. Thus, DPSCs have shown great potentiality to use in regenerative medicine for treatment of various human diseases including dental related problems. These cells can also be developed into induced pluripotent stem cells by incorporation of pluripotency markers and use for regenerative therapies of various diseases. The DPSCs are derived from various dental tissues such as human exfoliated deciduous teeth, apical papilla, periodontal ligament and dental follicle tissue. This review will overview the information about isolation, cellular and molecular characterization and differentiation of DPSCs into various types of human cells and thus these cells have important applications in regenerative therapies for various diseases. This review will be most useful for postgraduate dental students as well as scientists working in the field of oral pathology and oral medicine.展开更多
基金This work was supported by Beijing Natural Science Foundation （No. 6082005） and Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality （No. PXM2007014207）.
文摘An effective method for isolation of total RNA from pulps of mainly deciduous fruit trees was discussed in this paper. Here we shown that total RNA was successfully isolated from pulps of apple, peach and strawberry, but not grape berry via the effective CTAB method modified by ZENG, et al. However, total RNA was isolated from grape berry according to ZENG＇s protocols with a modification including 16% of β-Mercatoethanol and 3% of PVP in extraction buffer, 1/3 （V/V） of 5mol/L potassium acetate （KAC PH4.8） in supernatant. The detection of the isolated total RNA above using ultraviolet spectrophoto-metry, electrophoresis, reverse transcription and RT-PCR shown that the CTAB and its modifications is a preferred method for isolation of high-quality total RNA from fruit pulps.
文摘Amylolytic enzymes are currently investigated to improve industrial processes of starch degradation. Saccharomyces diastaticus 2047 isolated from cassava waste showed amylase and glucoamylase production, using starch medium, and the highest rate was obtained in the initial growth phase, after incubation for 24 h at pH 5.5. Maximum amylase and glucoamylase activities （483.62 U mg^-1 protein and 290.85 U mg^-1 protein） were obtained at pH 5.5. The isolated enzymes exhibited thermostable properties as indicated by retention of 100% of residual activity at 55 ℃ for 45 min with total inhibition at 100 ℃. Extracellular enzyme from S diastaticus 2047 was partially purified by fractionated precipitation with ammonium sulphate. After 40% saturation produced 2,197.00 and 1,192.83 U/mg protein, and yield was 40% with purification 4.54 and 4.1 fold, respectively. This study presents feasibility on ethanol production from cassava pulps pretreated with diluted sulfuric acid by simultaneous saccharification and fermentation （SSF） with S. diastaticus 2047. The results indicated that the culture was able to produce ethanol with high yield without amylolytic enzyme adding by using cassava pulps pretreated with distilled water at 135 ℃ under pressure of 15 lb/inch^2 to produce ethanol yield as high as the cassava pulps pretreated with diluted sulfuric acid under the same condition. This suggests that S diastaticus with enzyme produced has potential for industrial applications.
基金supported by The Thailand Science Research and Innovation(TSRI),Fundamental Fund 2022(Grant no FRB650070/0168)This research block grants was managed under Rajamangala University of Technology Thanyaburi(FRB65E0614).
文摘Carbon dots(CDs)have been attracted to nanocarbon materials for metal ion sensing,biological activity,and plant phytotoxicity due to their excellent photophysical properties,such as low cytotoxicity,high quantum yield,tunable fluorescence emission,and biocompatibility.Cassava pulp,which consists mainly of starch,has been identified as a low-cost biomass waste from the cassava starch industry.Therefore,this research developed CDs and nitrogen-doped CDs(NCDs)from cassava pulp using a one-step hydrothermal process in deionized water at 200℃.The effects of the synthesis conditions,including reaction time(6-24 h)and the nitrogen doping derivatives,were also investigated.CDs and ethylenediamine doped-NCDs exhibited tunable fluorescence emission,strong quantum yield,high photostability,and tolerance to photobleaching.Furthermore,the potential applications of CDs-12 h were demonstrated such as fluorescent sensors for metal ion sensing,antioxidant activity,and mercury detoxification in plants.Fluorescence quenching of the CDs-12 h via both static and dynamic quenching mechanisms was observed in the presence of several metal ions such as Hg^(2+),Cu^(2+),and Fe^(3+)with the detection limit in micromolar levels and further applied to real water samples with good recovery and acceptable relative standard derivation.The paper test strip coated with CDs-12 h could also detect these metal ions under UV light.CDs and NCDs-EDA also showed potential DPPH radical scavenging activity and alleviated mercury toxicity in the Chinese cabbage seedlings with the incubation of CDs-12 h and NCDs-EDA-12 h(30 mg/L).
基金supported by the National Natural Science Foundation of China(82170941 and 82370948 to Lu Zhang,82071110 and 82230029 to Zhi Chen)the National Key R&D Program of China(2018YFC1105100)。
文摘Pulpotomy,which belongs to vital pulp therapy,has become a strategy for managing pulpitis in recent decades.This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes.Pulpotomy is categorized into partial pulpotomy(PP),the removal of a partial segment of the coronal pulp tissue,and full pulpotomy(FP),the removal of whole coronal pulp,which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth.Procedural decisions for the amount of pulp tissue removal or retention depend on the diagnostic of pulp vitality,the overall treatment plan,the patient’s general health status,and pulp inflammation reassessment during operation.This statement represents the consensus of an expert committee convened by the Society of Cariology and Endodontics,Chinese Stomatological Association.It addresses the current evidence to support the application of pulpotomy as a potential alternative to root canal treatment(RCT)on mature permanent teeth with pulpitis from a biological basis,the development of capping biomaterial,and the diagnostic considerations to evidence-based medicine.This expert statement intends to provide a clinical protocol of pulpotomy,which facilitates practitioners in choosing the optimal procedure and increasing their confidence in this rapidly evolving field.
基金supported by the National Natural Science Foundation of China(32171347)the Foundation of Leading Talents from Shanghai Health Commission(2022XD038)+1 种基金Training Program for Research Physicians in Innovation,the Funda-mental Research Funds for the Central Universities(YG2023QNA23)Transforma-tion from shanghai hospital development center(SHDC2022CRD002).
文摘Stem cells play a crucial role in maintaining tissue regenerative capacity and homeostasis.However,mechanisms associated with stem cell senescence require further investigation.In this study,we conducted a proteomic analysis of human dental pulp stem cells(HDPSCs)obtained from individuals of various ages.Our findings showed that the expression of NUP62 was decreased in aged HDPSCs.We discovered that NUP62 alleviated senescence-associated phenotypes and enhanced differentiation potential both in vitro and in vivo.Conversely,the knocking down of NUP62 expression aggravated the senescence-associated phenotypes and impaired the proliferation and migration capacity of HDPSCs.Through RNA-sequence and decoding the epigenomic landscapes remodeled induced by NUP62 overexpression,we found that NUP62 helps alleviate senescence in HDPSCs by enhancing the nuclear transport of the transcription factor E2F1.This,in turn,stimulates the transcription of the epigenetic enzyme NSD2.Finally,the overexpression of NUP62 influences the H3K36me2 and H3K36me3 modifications of anti-aging genes(HMGA1,HMGA2,and SIRT6).Our results demonstrated that NUP62 regulates the fate of HDPSCs via NSD2-dependent epigenetic reprogramming.
基金supported by grants from the National Natural Science Foundation of China (82071104)Science and Technology Commission of Shanghai Municipality (23XD1434200/22Y21901000)+9 种基金Shanghai Hospital Development Center(SHDC12022120)National Clinical Research Center for Oral Diseases (NCRCO2021-omics-07)Shanghai Clinical Research Center for Oral Diseases (19MC1910600)Major and Key Cultivation Projects of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine (JYZP006)Shanghai’s Top Priority Research Center (2022ZZ01017)CAMS Innovation Fund for Medical Sciences (2019-I2M-5-037)Fundamental research program funding of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine(JYZZ237)Eastern Talent Plan Leading Project (BJZH2024001)partly supported by the Shanghai Ninth People’s Hospital affiliated with Shanghai Jiao Tong University,School of Medicine(JYJC202223)Shanghai Key Laboratory of Translational Medicine on Ear and Nose diseases (14DZ2260300)
文摘Pulpitis is a common infective oral disease in clinical situations.The regulatory mechanisms of immune defense in pulpitis are still being investigated.Osteomodulin(OMD)is a small leucine-rich proteoglycan family member distributed in bones and teeth.It is a bioactive protein that promotes osteogenesis and suppresses the apoptosis of human dental pulp stem cells(hDPSCs).In this study,the role of OMD in pulpitis and the OMD-induced regulatory mechanism were investigated.The OMD expression in normal and inflamed human pulp tissues was detected via immunofluorescence staining.Intriguingly,the OMD expression decreased in the inflammatory infiltration area of pulpitis specimens.The cellular experiments demonstrated that recombined human OMD could resist the detrimental effects of lipopolysaccharide(LPS)-induced inflammation.A conditional Omd knockout mouse model with pulpal inflammation was established.LPS-induced inflammatory impairment significantly increased in conditional Omd knockout mice,whereas OMD administration exhibited a protective effect against pulpitis.Mechanistically,the transcriptome alterations of OMD overexpression showed significant enrichment in the nuclear factor-κB(NF-κB)signaling pathway.Interleukin-1 receptor 1(IL1R1),a vital membrane receptor activating the NF-κB pathway,was significantly downregulated in OMD-overexpressing hDPSCs.Additionally,the interaction between OMD and IL1R1 was verified using co-immunoprecipitation and molecular docking.In vivo,excessive pulpal inflammation in Omd-deficient mice was rescued using an IL1R antagonist.Overall,OMD played a protective role in the inflammatory response via the IL1R1/NF-κB signaling pathway.OMD may optimize the immunomodulatory functions of hDPSCs and can be used for regenerative endodontics.
基金funded by the National Key R&D Program of China(2022YFC2405904)the National Natural Science Foundation of China(52272284,32225028)+1 种基金Joint Research Unit Plan of the Chinese Academy of Sciences(121631ZYLH20240014)the Science and Technology Commission of Shanghai Municipality(24520750100)。
文摘Dental pulp-dentin complex defects remain a major unresolved problem in oral medicines.Clinical therapeutic methods including root canal therapy and vital pulp therapy are both considered as conservative strategies,which are incapable of repairing the pulpdentin complex defects.Although biomaterial-based strategies show remarkable progress in antibacterial,anti-inflammatory,and pulp regeneration,the important modulatory effects of nerves within pulp cavity have been greatly overlooked,making it challenging to achieve functional pulp-dentin complex regeneration.In this study,we propose an injectable bioceramicscontaining composite hydrogel in combination of Li-Ca-Si(LCS)bioceramics and gelatin methacrylate matrix with photocrosslinking properties.Due to the sustained release of bioactive Li,Ca and Si ions from LCS,the composite hydrogels possess multiple functions of promoting the neurogenic differentiation of Schwann cells,odontogenic differentiation of dental pulp stem cells,and neurogenesis-odontogenesis couples in vitro.In addition,the in vivo results showed that LCS-containing composite hydrogel can significantly promote the pulp-dentin complex repair.More importantly,LCS bioceramics-containing composite hydrogel can induce the growth of nerve fibers,leading to the re-innervation of pulp tissues.Taken together,the study suggests that LCS bioceramics can induce the innervation of pulp-dentin complex repair,offering a referable strategy of designing multifunctional filling materials for functional periodontal tissue regeneration.
文摘On January 3:Hubei Xianhe New Materials Co.,Ltd.Phase I project was officially put into production,with a total investment of 10 billion CNY and a planned pulp and paper production capacity of 2.5 million tons per year.On January 10:The pulp and paper project of Huatai Group is accelerating construction.With a total investment of 10.2 billion CNY,after completion,Huatai's newsprint production capacity will be reduced from 1.4 million tons per year to 400000 tons per year,while cultural paper capacity will increase from 100000 tons per yeart to 1.6 million tons per year,achieving annual sales revenue of 19 billion CNY and profits and taxes of 3.2 billion CNY.
基金funded by the Agricultural Science and Technology Innovation Fund of Jiangsu Province[Grant/Award Number:CX(24)1008]Key Research and Development Program Project of Henan Province(251111110100)National State Science Foundation of China[Grant/Award Number:21808093].
文摘Long-time fermentation has always been one of the reasons restricting the development of straw biological pulping.This study aimed to develop a novel straw pulp film with shortened solid-state fermentation time with less than 20%mass loss rate by bio-pulping synergistic treatment of straw fibers with deep eutectic solvent(DES)and Streptomyces rochei(S.rochei).Results illustrated that at 3%S.rochei concentration with 7-day fermentation,both cellulose and hemicellulose enzyme activities of the treated rice straw fiber reached peak values with a fiber mass loss rate of 17.01%.Microstructural morphology revealed that S.rochei colonization initiated on straw surfaces and progressively penetrated internal structures,resulting in surface loosening and distinct disruption of cell wall tissues within vascular bundles in transverse sections.The treated rice straw strip indicated a maximum tensile strength of 46.22 MPa for(Bacteria)BA 3%at day 7,attributed to optimized synergistic effects of microfibril angle(MFA)and cellulose/hemicellulose relative content ratio.The modified straw pulp film exhibited significant enhancement in the tensile index(44.9%increase),burst index(10.3%increase),and tear index(60%increase)compared to untreated groups.This work demonstrated the important role ofDES and S.rochei bio-pulping synergistic treatment in improving rice straw pulp performance,suggesting an eco-friendly,novel,and efficient biomass pretreatment technology for potential application prospects in sustainable agricultural mulching materials.
文摘Circulose,a global leader in recycled pulp production,hasreached a strategic partnership agreement with Tangshan SanyouChemical Fiber,a front-runner in the cellulose fiber industry.Thiscollaboration marks a significant milestone in the commercializa-tion and scaling of CIRCULOSER,whle also propelling TangshanSanyou's brand toward greater international recognition.
基金supported by the Zhejiang Key R&D Program of China(2020C02037)the Ningbo Agricultural Research Program of China(2022S1542022S138).
文摘Peach are a fruit with high nutritional and economic value,but their safety and suitability for diabetic patients have been questioned.This study investigated the effects and potential mechanisms of peach pulp(PP)on type 2 diabetes mellitus(T2DM)in mice induced by a high-fat diet(HFD)combined with streptozotocin(STZ).The results showed that PP alleviated hyperglycemia,hyperlipidemia,hyperuricemia,and tissue dysfunction in T2DM mice through the synergistic effect of nutrients and non-nutrient compounds.Analysis of mRNA expression levels revealed that PP improved glucose metabolism in T2DM mice by promoting glycogen synthesis and inhibiting gluconeogenesis.Furthermore,elevated levels of PP resulted in an increase in acetic acid content following a 4 weeks intervention period.Additionally,it led to the restoration of gut microbiota balance by decreasing the Firmicutes/Bacteroidota(F/B)ratio and enhancing the presence of Romboutsia,Allobaculum,Alloprevotella,and Bacteroides after an 8 weeks intervention.Ultimately,our results suggest that PP may offer advantages for individuals with diabetes.
基金ORCID number:Qing-Feng Wang 0009-0009-4579-7133Ying Wu 0009-0007-2061-739X。
文摘BACKGROUND The two-way,three-stage psychological intervention for patients with dental pulp disease offers a reference for nursing interventions in such cases.AIM To examine the effects of a three-stage psychological intervention on psychological resilience and health behaviors.METHODS A total of 114 patients with dental pulp disease treated between December 2022 and December 2023 were allocated into two groups according to the random lottery method,with 57 patients in each group.The control group adopted the teaching method,while the observation group used a three-stage psychological intervention combined with the teaching method.We compared psychological resilience,coping strategies,dental fear,health behavior habits,and stigma between the two groups.RESULTS The intervention group showed significantly improved scores on the psychological resilience scale(Connor-Davidson Resilience Scale)(P<0.05);positive and negative response scores also improved after the intervention(P<0.05);significant differences were observed between the observation and control groups in the Chinese version of the Stouthard Dental Fear Scale(Dental Anxiety Inventory),the Social Impact Scale,and health behavior score(P<0.05).CONCLUSION Combining a three-stage psychological intervention with the back-teaching method effectively reduces dental fear and stigma in patients with dental pulp disease.It also improves psychological resilience,coping strategies,and health behavior habits,achieving significant results.
基金funded by the LatvianCouncil of Science,the project“Investigation of Eco-Friendly Thermal Insulation Materials from Sustainable and Renewable Industrial Crops Residuals,”number lzp-2021/1-0599.
文摘This study examines the development of loose-fill thermal insulation materials derived from annual plant residues,such as wheat straw,water reeds,and corn stalks,processed using the chemimechanical pulping(CMP)technique.The chopped plants were soda-cooked for 30 min,varying NaOH concentration(2%–8%on a dry basis of biomass),and mechanically refined using different disc types.The CMPprocess enhances the homogeneity and stability of defibratedmaterial,yielding improved insulation properties compared to untreated chopped rawmaterials.Chemical analysis revealed that CMP increases cellulose content and reduces lignin levels,enhancing water retention and vapor diffusion properties.Settlement tests confirmed that CMP materials are more resistant to compaction under vibration,maintaining long-term performance.Additionally,the CMP enables the production of lightweight materials that require less resource consumption while achieving comparable thermal insulation performance.The investigated biobased materials offer a sustainable alternative to conventional insulation,with competing thermal conductivity values(0.041-0.046 W/mK)at the settlement-resistant bulk density level of 60 kg/m^(3).The thermal conductivity of CMP materials remains minimally affected.However,the resulting fibers demonstrate significant advantages in stability and material efficiency.This highlights its suitability for loose-fill applications to improve the sustainability of the construction.Using renewable plant residues,CMP-based insulation materials align with circular economy principles and contribute to environmental sustainability.This research underscores the potential of CMP materials to reduce greenhouse gas emissions,optimize resource use,and promote eco-friendly building practices.
文摘From August 12 to 15,the 2025 China International Paper Technology Exhibition(hereina?t er referred to as“CIPTE”)was successfully held in Shanghai.Hosted by the China Paper Association,China Technical Association of Paper Industry,and China National Pulp and Paper Research Institute Co.,Ltd.(below referred to as“CNPPRI”),and organized by China Pulp and Paper Magazines Publisher,this event received strong support and extensive attention from industry peers.
基金supported through funding from Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515030036)the National Natural Science Foundation of China(Grant No.82270973)。
文摘Tooth pulpitis is a prevalent oral disorder.Understanding the underlying mechanisms of pulpitis and developing effective treatment strategies hold great significance.Ferroptosis has recently emerged as a new form of cell death,but the role of ferroptosis in pulpitis remains largely unknown.In our study,single-cell RNA sequencing(sc RNA-seq)was used to identify cellular heterogeneity between 3 pulpitis tissue and 3 healthy pulp tissue,and explored ferroptosis occurrence in pulpitis tissue and inflamed dental pulp cells(DPCs).In scRNA-seq,40231 cells(Pulpitis:17814;Healthy pulp:22417)were captured,and visualized into 12 distinct cell clusters.Differential y expressed ferroptosis-related genes(DE-FRGs)were almost presented in each cluster in pulpitis vs healthy pulp.ROS and Fe^(2+)levels significantly rose,and immunohistochemistry showed low expression of GPX4 and high expression of PTGS2 in pulpitis.In LPSstimulated DPCs,thymosinα1 increased the expression of GPX4 and FTL,and decreased expression of TNF-α,IL-1β,IL-6,and Fe^(2+)levels.In rat pulpitis models,both prothymosinα(PTMA,precursor of thymosinα1)gelatin sponge placed at the hole of pulp(LPS-P(gs))and PTMA injection in pulp(LPS-P(i))significantly reduced infiltration of inflammatory cells and expression of PTGS2,and increased the expression of GPX4.In RNA sequencing,the expression of DE-FRGs were reversed when thymosinα1 were added in LPS-stimulated DPCs.Collectively,single-cell atlas reveals cellular heterogeneity between pulpitis and healthy pulp,and ferroptosis occurrence in pulpitis.Thymosinα1 may reduce ferroptosis in DPCs to alleviate pulpitis and thus potentially has the ability to treat pulpitis.
基金This study was sponsored by the Research Funding for Outstanding Young University Faculty of China Ministry of Education (No. 2001-39), Fujian Provincial Innovation Fundation for Young Science and Technology Talents (No. 2004J012), and the National Natural Science Funda-tion of China (No. 30571461)
文摘The rheological behavior of low consistency thermomechanical pulp of Chinese fir harvested by intermediate thinning was analyzed. The results show that the apparent viscosity of pulp changed along with the beating degree, pulp consistency and shearing velocity. With the increasing of pulp consistency, the apparent viscosity of pulp increased gradually. Beating degree of pulp had an effect on microstructure of pulp. The apparent viscosity of pulp declined as beating degree of pulp increased, and the apparent viscosity of pulp fell along with the shearing velocity increasing. Based on the results, the rheological models are set up. The models showed that the fluid types of the low consistency pulp could be described as pseudoplastics fluids （non-Newtonian fluids）.
基金the Key Scientific and Technological Project of Heilongjiang Province (GB06B501-1)
文摘Cationized pulp fibers （CPF） were prepared by the adsorption of a novel biodegradable cationic ester quaternary ammonium salt （31441） on bleached softwood kraft pulp fibers. The optimized conditions for the CPF preparation were： 4% of 31441 （based on oven-dry pulp）, 80℃ and 30 rain. The CPF was characterized by FT-IR, SEM and XPS. Experimental results showed that the CPF improved the retention of precipitated calcium carbonate （PCC） filler significantly. With 0.9% CPF （based on oven-dry pulp）, the retention of PCC increased from 57.53% to 72.21%. The physical properties of paper were also slightly improved. The tensile strength and burst strength of the paper with CPF were higher than those with CPAM. CPF addition had no effect on the stock drainage.
基金supported by RC2DE020767 from the National Institute of Dental and Craniofacial Research (NIDCR), the National Institutes of Health (NIH)
文摘A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated？ Can adult stem cells be orchestrated to regenerate tooth structures such as the enamel, dentin, cementum and dental pulp, or even an entire tooth？ If not, what are the therapeutically viable sources of stem cells for tooth regeneration？ Do stem cells necessarily need to be taken out of the body, and manipulated ex vivo before they are transplanted for tooth regeneration？ How can regenerated teeth be economically competitive with dental implants？ Would it be possible to make regenerated teeth affordable by a large segment of the population worldwide？ This review article explores existing and visionary approaches that address some of the above-mentioned questions. Tooth regeneration represents a revolution in stomatology as a shift in the paradigm from repair to regeneration： repair is by metal or artificial materials whereas regeneration is by biological restoration. Tooth regeneration is an extension of the concepts in the broad field of regenerative medicine to restore a tissue defect to its original form and function by biological substitutes.
基金financially supported by the National Natural Science Foundation of China(Nos.51504053 and 51374079)the Fundamental Research Funds for the Central Universities(No.N170107013)
文摘Given the gradual increase in the chlorite content of hematite ores, pulp properties seriously deteriorate during flotation. The traditional anion reverse flotation of hematite cannot effectively eliminate the effects of chlorite, leading to a significant decrease in the total Fe(TFe) grade of the concentrate. In this work, the effect of sodium alginate on the reverse flotation of hematite was systematically investigated. Flotation tests of artificially mixed ores were conducted, and the results showed that sodium alginate can significantly improve the removal rates of quartz and chlorite. The adsorption measurements, infrared spectroscopy, and contact angle tests demonstrated that sodium alginate adsorbs on the quartz surface by chelating with calcium ions, thereby weakening the steric hindrance of oleate ions and increasing the adsorption capacity of sodium oleate to ultimately improve the removal rate of quartz. Furthermore, owing to its lower density and fine particle size, chlorite is easily entrained into the foam layer. Sodium alginate dramatically increases the liquid-to-gas ratio of the foam layer by increasing pulp viscosity, thereby increasing the entrainment rate of chlorite and finally improving its removal rate. The core content of this thesis bears significance in improving the Fe grade in the reverse flotation of chlorite-containing hematite.
基金Supported by Jaslok Hospital and Research Centre,Mumbai,India,Project ni491,A/C 27814
文摘Stem cells are pluripotent cells, having a property of differentiating into various types of cells of human body. Several studies have developed mesenchymal stem cells(MSCs) from various human tissues,peripheral blood and body fluids. These cells are then characterized by cellular and molecular markers to understand their specific phenotypes. Dental pulp stem cells(DPSCs) are having a MSCs phenotype and they are differentiated into neuron, cardiomyocytes, chondrocytes, osteoblasts, liver cells and β cells of islet of pancreas. Thus, DPSCs have shown great potentiality to use in regenerative medicine for treatment of various human diseases including dental related problems. These cells can also be developed into induced pluripotent stem cells by incorporation of pluripotency markers and use for regenerative therapies of various diseases. The DPSCs are derived from various dental tissues such as human exfoliated deciduous teeth, apical papilla, periodontal ligament and dental follicle tissue. This review will overview the information about isolation, cellular and molecular characterization and differentiation of DPSCs into various types of human cells and thus these cells have important applications in regenerative therapies for various diseases. This review will be most useful for postgraduate dental students as well as scientists working in the field of oral pathology and oral medicine.
