[Objectives]To distinguish loquat flesh color,thereby guiding early-age selection in loquat hybrid progeny.[Methods]Molecular markers based on the phytoene synthase gene(PSY)of loquat were applied to a hybrid progeny ...[Objectives]To distinguish loquat flesh color,thereby guiding early-age selection in loquat hybrid progeny.[Methods]Molecular markers based on the phytoene synthase gene(PSY)of loquat were applied to a hybrid progeny population derived from a cross between‘Zaozhong 6’and a local wild white-fleshed loquat resource‘DB1’.[Results]Among the 49 hybrid progeny,24 were identified as white-fleshed loquat resources and 25 as yellow-fleshed loquat resources.[Conclusions]The molecular marker of the PSY gene can effectively distinguish loquat flesh color and is of significant importance for guiding early-age selection in loquat hybrid breeding.展开更多
Carotenoids are lipophilic isoprenoid pigments with essential roles in plants.While the cultivated allotetraploid cottons exhibit distinct mature anther coloration—yellow in Gossypium barbadense versus predominantly ...Carotenoids are lipophilic isoprenoid pigments with essential roles in plants.While the cultivated allotetraploid cottons exhibit distinct mature anther coloration—yellow in Gossypium barbadense versus predominantly white in G.hirsutum—the genetic basis of this divergence remains unclear.The purpose of this study was to identify the genetic basis of anther-color variation in cotton(Gossypium)species.We firstly identified carotenoids as the primary pigments underlying yellow-anthers coloration.Comparative transcriptomics of anthers revealed that the carotenoid biosynthesis gene GbPSY4 was expressed as a key regulator in G.barbadense.Functional validation via tissue-specific expression,subcellular localization,in vivo enzymatic assays,and virus-induced gene silencing confirmed its role in carotenoid biosynthesis and yellow pigmentation.Genome-wide association studies in a G.hirsutum population revealed GhPSY4_At,an ortholog of GbPSY4,as the causal gene of anther-color variation.We conclude that PSY4-regulated carotenoid biosynthesis governs yellow pigmentation.Furthermore,a finding that G.hirsutum accessions with yellow anthers showed greater pollen viability under high-temperature stress than those with white anthers suggests that the same pathway that governs yellow pigmentation influences heat tolerance.PSY4 is a promising target for breeding stress-tolerant cotton varieties.展开更多
基金Supported by Chengdu Science and Technology ProjectResearch and Development of Key Production Technologies and Scientific and Technological Services for Characteristic Fruit Trees in Lezhi County,Ziyang City(Science and Technology Commissioner)(2025-YF05-00549-SN).
文摘[Objectives]To distinguish loquat flesh color,thereby guiding early-age selection in loquat hybrid progeny.[Methods]Molecular markers based on the phytoene synthase gene(PSY)of loquat were applied to a hybrid progeny population derived from a cross between‘Zaozhong 6’and a local wild white-fleshed loquat resource‘DB1’.[Results]Among the 49 hybrid progeny,24 were identified as white-fleshed loquat resources and 25 as yellow-fleshed loquat resources.[Conclusions]The molecular marker of the PSY gene can effectively distinguish loquat flesh color and is of significant importance for guiding early-age selection in loquat hybrid breeding.
基金the National Natural Science Foundation of China(32170271,32470277)the Project of Sanya Yazhou Bay Science and Technology City(SCKJ-JYRC-2023-52)the Natural Science Foundation of Henan Province(252300421076,222300420024).
文摘Carotenoids are lipophilic isoprenoid pigments with essential roles in plants.While the cultivated allotetraploid cottons exhibit distinct mature anther coloration—yellow in Gossypium barbadense versus predominantly white in G.hirsutum—the genetic basis of this divergence remains unclear.The purpose of this study was to identify the genetic basis of anther-color variation in cotton(Gossypium)species.We firstly identified carotenoids as the primary pigments underlying yellow-anthers coloration.Comparative transcriptomics of anthers revealed that the carotenoid biosynthesis gene GbPSY4 was expressed as a key regulator in G.barbadense.Functional validation via tissue-specific expression,subcellular localization,in vivo enzymatic assays,and virus-induced gene silencing confirmed its role in carotenoid biosynthesis and yellow pigmentation.Genome-wide association studies in a G.hirsutum population revealed GhPSY4_At,an ortholog of GbPSY4,as the causal gene of anther-color variation.We conclude that PSY4-regulated carotenoid biosynthesis governs yellow pigmentation.Furthermore,a finding that G.hirsutum accessions with yellow anthers showed greater pollen viability under high-temperature stress than those with white anthers suggests that the same pathway that governs yellow pigmentation influences heat tolerance.PSY4 is a promising target for breeding stress-tolerant cotton varieties.
