Glutathione S-transferases（GSTs） from Liposcelis bostrychophila Badonnel and L.entomophila（Enderlein）（Psocoptera：Liposcelididae） were purified by glutathione-agarose affinity chromatography,and characterized su...Glutathione S-transferases（GSTs） from Liposcelis bostrychophila Badonnel and L.entomophila（Enderlein）（Psocoptera：Liposcelididae） were purified by glutathione-agarose affinity chromatography,and characterized subsequently by their Michaelis-Menten kinetics toward the artificial substrates 1-chloro-2,4-dinitrobenzene（CDNB） and reduced glutathione（GSH）,respectively.The specific activity of the purified GST toward CDNB was 2.3-fold higher in L.bostrychophila than in L.entomophila.Though the specific activities of purified enzymes varied between the two species,the purification yields were similar.SDS-PAGE revealed one band at 23 kDa for both the species.GSTs of L.entomophila exhibited higher Michaelis-Menten constants（Km） but lower maximal velocity（Vmax） values than those of L.bostrychophila.The optimum pH for CDNB conjugation of L.bostrychophila and L.entomophila GSTs was 7.0 and 7.5,and optimum temperature was 35 and 40°C,respectively.Inhibition kinetics showed that cibacron blue,curcumin,bromosulfalein,ethacrynic acid,and carbosulfan had excellent inhibitory effects on GSTs in both species,but the inhibitory effects of beta-cypermethrin,fenpropathrin,tetraethylthiuram disulfide,and diethyl maleate were not significant.展开更多
Psocodean species are emerging as significant sanitary and stored-product pests,posing threats to human health and global food security.Out of an estimated 10000 species,the whole genome sequences of only 4 species ha...Psocodean species are emerging as significant sanitary and stored-product pests,posing threats to human health and global food security.Out of an estimated 10000 species,the whole genome sequences of only 4 species have been published.Genomic resources are crucial for establishing effective pest control and enhancing our understanding of the evolution of psocodean species.In this study,we employed Illumina and PacBio sequencing along with Hi-C scaffolding techniques to generate a chromosome-level genome assembly for the parthenogenetic booklouse Liposcelis bostrychophila.The assembled genome of this booklouse measures 291.67 Mb in length and comprises 9 chromosomes.Notably,the genome of L.bostrychophila exhibits a high level of heterozygosity and features a distinctive nonhomologous chromosome.This heterozygous characteristic of the parthenogenetic booklouse genome may arise from high mutation rates,based on genomic variations analysis across multiple generations.Our analysis revealed significantly expanded gene families,primarily associated with the detoxification and feeding habits of L.bostrychophila.These include integument esterases(ESTs),ATP-binding cassette(ABC)transporter genes and gustatory receptors(GRs).The high-quality genome sequence of L.bostrychophila provides valuable resources for further study on the molecular mechanisms of stress resistance.It enables researchers to identify crucial functional genes and facilitates research on the population genetics,evolution and phylogeny of booklice.展开更多
基金funded in part by the National Natural Sciences Foundation of China (30871631)the Specialized Research Fund for the Doctoral Program of Higher Education of China to Prof. Wang Jinjun(200806350009)+1 种基金the Doctoral Program of Southwest University of China (SWU109023)the Fundamental Research Funds for the Central Universities,China(XDJK2009C112)
文摘Glutathione S-transferases（GSTs） from Liposcelis bostrychophila Badonnel and L.entomophila（Enderlein）（Psocoptera：Liposcelididae） were purified by glutathione-agarose affinity chromatography,and characterized subsequently by their Michaelis-Menten kinetics toward the artificial substrates 1-chloro-2,4-dinitrobenzene（CDNB） and reduced glutathione（GSH）,respectively.The specific activity of the purified GST toward CDNB was 2.3-fold higher in L.bostrychophila than in L.entomophila.Though the specific activities of purified enzymes varied between the two species,the purification yields were similar.SDS-PAGE revealed one band at 23 kDa for both the species.GSTs of L.entomophila exhibited higher Michaelis-Menten constants（Km） but lower maximal velocity（Vmax） values than those of L.bostrychophila.The optimum pH for CDNB conjugation of L.bostrychophila and L.entomophila GSTs was 7.0 and 7.5,and optimum temperature was 35 and 40°C,respectively.Inhibition kinetics showed that cibacron blue,curcumin,bromosulfalein,ethacrynic acid,and carbosulfan had excellent inhibitory effects on GSTs in both species,but the inhibitory effects of beta-cypermethrin,fenpropathrin,tetraethylthiuram disulfide,and diethyl maleate were not significant.
基金supported by the National Natural Science Foundation of China(31972276 and 32272528)the Special Fund for Youth Team of the Southwest Universities(SWU-XJLJ202304).
文摘Psocodean species are emerging as significant sanitary and stored-product pests,posing threats to human health and global food security.Out of an estimated 10000 species,the whole genome sequences of only 4 species have been published.Genomic resources are crucial for establishing effective pest control and enhancing our understanding of the evolution of psocodean species.In this study,we employed Illumina and PacBio sequencing along with Hi-C scaffolding techniques to generate a chromosome-level genome assembly for the parthenogenetic booklouse Liposcelis bostrychophila.The assembled genome of this booklouse measures 291.67 Mb in length and comprises 9 chromosomes.Notably,the genome of L.bostrychophila exhibits a high level of heterozygosity and features a distinctive nonhomologous chromosome.This heterozygous characteristic of the parthenogenetic booklouse genome may arise from high mutation rates,based on genomic variations analysis across multiple generations.Our analysis revealed significantly expanded gene families,primarily associated with the detoxification and feeding habits of L.bostrychophila.These include integument esterases(ESTs),ATP-binding cassette(ABC)transporter genes and gustatory receptors(GRs).The high-quality genome sequence of L.bostrychophila provides valuable resources for further study on the molecular mechanisms of stress resistance.It enables researchers to identify crucial functional genes and facilitates research on the population genetics,evolution and phylogeny of booklice.
