Pseudosolubilized ability of Pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n- octadecane were studied by analyzing amount of pseudo- solubil...Pseudosolubilized ability of Pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n- octadecane were studied by analyzing amount of pseudo- solubilized oil components in water phase, and the fraction of radiolabeled ~4C n-octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C12 to C28 of n-alkanes. In n- octadecane broth, pseudosolubilized n-octadecane could be accumulated as long as faster than mineralization maximum concentration of cane achieved to 45.37 mg. L pseudosolubilized rate was rate of substrate, and the pseudosolubilized n-octade- 1. All of these results showed that Pseudomonas sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of 14C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of n- octadecane.展开更多
文摘Pseudosolubilized ability of Pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n- octadecane were studied by analyzing amount of pseudo- solubilized oil components in water phase, and the fraction of radiolabeled ~4C n-octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C12 to C28 of n-alkanes. In n- octadecane broth, pseudosolubilized n-octadecane could be accumulated as long as faster than mineralization maximum concentration of cane achieved to 45.37 mg. L pseudosolubilized rate was rate of substrate, and the pseudosolubilized n-octade- 1. All of these results showed that Pseudomonas sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of 14C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of n- octadecane.
