目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling fo...目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling for 10,20,30 min and then drying,B10D、B20D、B30D)、隔水蒸制10、20、30 min后烘干(steaming for 10,20,30 min and then drying,S10D、S20D、S30D)和冷冻干燥(freeze drying,FD)共8种方式处理独蒜兰假鳞茎,测定其2-O-葡萄糖基白及苷(dactylorhin A)、白及苷和山药素Ⅲ的含量。通过构建HPLC指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)和主成分分析(principal component analysis,PCA)评价加工方法对假鳞茎质量的影响。通过外观特征、组织结构及粉末显微结构观察,评估加工方法对假鳞茎结构的影响。以dactylorhin A、白及苷、山药素Ⅲ、醇溶性浸出物、水溶性浸出物、折干率、水分、灰分和酸不溶性灰分含量为指标,利用熵权法、层次分析法(analytic hierarchy process,AHP)和逼近理想解排序法(technique for order preference by similarity to ideal solution,TOPSIS)综合评价不同加工方法对假鳞茎品质的影响。结果8种加工方法所得样品的HPLC指纹图谱相似度为0.681~0.994。其中,FD样品的相似度最低,且HCA和PCA均将其单独归为一类,其余7种加工方法的样品则聚为另一类。通过HPLC指纹图谱24个共有峰鉴定出3个共有峰,分别为dactylorhin A(峰7)、白及苷(峰9)和山药素Ⅲ(峰15)。TOPSIS综合评价结果发现,S10D处理样品的贴近度以及dactylorhin A、白及苷和山药素Ⅲ总含量最高。结论隔水蒸制10 min后烘干是独蒜兰假鳞茎初加工的最优方法,该方法有利于活性成分保留,且工艺简便。为独蒜兰假鳞茎的规范化加工以及山慈菇药材质量标准的完善提供了重要依据。展开更多
An efficient procedure is outlined for rapid and mass propagation through in vitro culture of pseudobulbs collected in different seasons of an endangered orchid, Changnienia amoena Chien. Axillary buds formed on intac...An efficient procedure is outlined for rapid and mass propagation through in vitro culture of pseudobulbs collected in different seasons of an endangered orchid, Changnienia amoena Chien. Axillary buds formed on intact pseudobulbs (collected in April) after a 12-week incubation on half-strength Murashige and Skoog (1/2 MS) medium supplemented with 1 mg L-1 N6-benzyl- adenine (6-BA), 0.5 mg L-1 α-naphthaleneacetic acid (NAA), 100 ml L-1 coconut water and 0.8 g L-1 polyvinylpyrrolidone; no buds were observed on segmentalized pseudobulbs incubated on the same medium. The axillary buds obtained from pseudobulbs growing in the natural habitat in June were detached and incubated for 7 weeks on the same medium leading to 1.4 shoot buds per explant. With repeated subculturing of the shoots on 1/2 MS medium supplemented with 2 mg L-1 6-BA and 0.5 mg L-1 NAA, a mean of 3.3 shoot buds per explant were observed on successive shoot cultures. A mean of 4.5 roots per shoot were induced on the optimal root induction medium with 1/2 MS medium plus 1.0 mg L-1 NAA and 0.1 mg L-1 6-BA and the highest rooting per centage was 88.9%. Plantlets 4-5 cm in height were transplanted into pots containing a 1:1 humus and sand mixture and grown for 7 weeks in a greenhouse before being transferred to the field. The survival rate of these transplants was about 75% after two months of growth in the wild.展开更多
文摘目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling for 10,20,30 min and then drying,B10D、B20D、B30D)、隔水蒸制10、20、30 min后烘干(steaming for 10,20,30 min and then drying,S10D、S20D、S30D)和冷冻干燥(freeze drying,FD)共8种方式处理独蒜兰假鳞茎,测定其2-O-葡萄糖基白及苷(dactylorhin A)、白及苷和山药素Ⅲ的含量。通过构建HPLC指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)和主成分分析(principal component analysis,PCA)评价加工方法对假鳞茎质量的影响。通过外观特征、组织结构及粉末显微结构观察,评估加工方法对假鳞茎结构的影响。以dactylorhin A、白及苷、山药素Ⅲ、醇溶性浸出物、水溶性浸出物、折干率、水分、灰分和酸不溶性灰分含量为指标,利用熵权法、层次分析法(analytic hierarchy process,AHP)和逼近理想解排序法(technique for order preference by similarity to ideal solution,TOPSIS)综合评价不同加工方法对假鳞茎品质的影响。结果8种加工方法所得样品的HPLC指纹图谱相似度为0.681~0.994。其中,FD样品的相似度最低,且HCA和PCA均将其单独归为一类,其余7种加工方法的样品则聚为另一类。通过HPLC指纹图谱24个共有峰鉴定出3个共有峰,分别为dactylorhin A(峰7)、白及苷(峰9)和山药素Ⅲ(峰15)。TOPSIS综合评价结果发现,S10D处理样品的贴近度以及dactylorhin A、白及苷和山药素Ⅲ总含量最高。结论隔水蒸制10 min后烘干是独蒜兰假鳞茎初加工的最优方法,该方法有利于活性成分保留,且工艺简便。为独蒜兰假鳞茎的规范化加工以及山慈菇药材质量标准的完善提供了重要依据。
基金supported by Zhejiang Provincial Natural Science Foundation (Y507195)the National Key Project of Scientific and Technical Sup-porting Programs Funded by Ministry of Science & Technology of China (2008BAC39B05)
文摘An efficient procedure is outlined for rapid and mass propagation through in vitro culture of pseudobulbs collected in different seasons of an endangered orchid, Changnienia amoena Chien. Axillary buds formed on intact pseudobulbs (collected in April) after a 12-week incubation on half-strength Murashige and Skoog (1/2 MS) medium supplemented with 1 mg L-1 N6-benzyl- adenine (6-BA), 0.5 mg L-1 α-naphthaleneacetic acid (NAA), 100 ml L-1 coconut water and 0.8 g L-1 polyvinylpyrrolidone; no buds were observed on segmentalized pseudobulbs incubated on the same medium. The axillary buds obtained from pseudobulbs growing in the natural habitat in June were detached and incubated for 7 weeks on the same medium leading to 1.4 shoot buds per explant. With repeated subculturing of the shoots on 1/2 MS medium supplemented with 2 mg L-1 6-BA and 0.5 mg L-1 NAA, a mean of 3.3 shoot buds per explant were observed on successive shoot cultures. A mean of 4.5 roots per shoot were induced on the optimal root induction medium with 1/2 MS medium plus 1.0 mg L-1 NAA and 0.1 mg L-1 6-BA and the highest rooting per centage was 88.9%. Plantlets 4-5 cm in height were transplanted into pots containing a 1:1 humus and sand mixture and grown for 7 weeks in a greenhouse before being transferred to the field. The survival rate of these transplants was about 75% after two months of growth in the wild.
