Banana pseudo-stem was liquefied in the mixture of polyhydric alcohols of polyethylene glycol(PEG400) and glycerol. Hydroxyl value of liquefied products ranged from 294.8 to 370.2 mg KOH/g and M_n was about 430. Lique...Banana pseudo-stem was liquefied in the mixture of polyhydric alcohols of polyethylene glycol(PEG400) and glycerol. Hydroxyl value of liquefied products ranged from 294.8 to 370.2 mg KOH/g and M_n was about 430. Liquefied products(LBPP) could be used as raw materials for polyurethane by reacting with 4, 4'-diphenylmethane diisocyanate(4, 4'-MDI) and PEG400 to synthesize liquefied product-based polyurethane(LBPP-PU) adhesive. To analyze in depth the creation of urethane linkage among LBPP, PEG400 and 4, 4'-MDI, factors which had effects on the residue content were all investigated. They were characterized by FT-IR and TG. The shear strength of LBPP-PU adhesive was improved when decreasing the percentage of the substitution of PEG400 by LBPP. The adhesive strength was obtained from T-peel of aspen/polyurethane adhesive joints, and the maximum lap shear strength(4.40 MPa) was obtained when 16.70% of LBPP was added to the LBPP & PEG400 system.展开更多
Banana(Musa spp.)is an ancient and popular fruit plant with highly nutritious fruit.The pseudo-stem of banana represents on average 75%of the total dry mass but its valorization as a nutritional and industrial by-prod...Banana(Musa spp.)is an ancient and popular fruit plant with highly nutritious fruit.The pseudo-stem of banana represents on average 75%of the total dry mass but its valorization as a nutritional and industrial by-product is limited.Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts.This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem.Hereby,we identified and quantified 373 metabolites from a diverse range of classes including,alkaloids,flavonoids,lipids,phenolic acids,amino acids and its derivatives,nucleotide and its derivatives,organic acids,lignans and coumarins,tannins,and terpene using the widely-targeted metabolomics approach.Banana pseudo-stem is enriched in metabolites for utilization in the food industry(L-lysine and L-tryptophan,L-glutamic acid,Phenylalanine,Palmitoleic acid,α-Linolenic acid,and Lauric acid,and Adenine)and pharmaceutical industry(Guanosine and Cimidahurinine,Bergapten,Coumarins,Procyanidin A2,Procyanidin B1,Procyanidin B3,Procyanidin B2,and Procyanidin B4,Asiatic acid).The metabolome of banana pseudo-stem with integration across multiomics data may provide the opportunity to exploit the rich metabolome of banana pseudo-stem for industrial and nutritional applications.展开更多
The measurement of banana pseudo-stem phenotypic parameters is a critical way to evaluate the growth status of bananas,and it can provide essential data support for mechanized cultivation operations such as fertilizat...The measurement of banana pseudo-stem phenotypic parameters is a critical way to evaluate the growth status of bananas,and it can provide essential data support for mechanized cultivation operations such as fertilization and pesticide application.Existing studies mainly measure the diameter of banana pseudo-stem as its phenotypic parameter.The banana pseudo-stem cross section was closer to an ellipse other than a standard circle,so the diameter parameter cannot adequately represent the phenotypic characteristics of the banana plant.In this study,an automatic measuring device for banana pseudo-stem phenotypic parameters was developed.The device,which integrates three different types of sensors:a laser ranging sensor,a rotary encoder,and a digital camera,were used to obtain the point cloud and image data of banana pseudo-stem.A K-means point clouds clustering algorithm based on Euclidean distance was proposed.The point cloud of banana pseudo-stem was identified and extracted.A three-dimensional reconstruction algorithm based on the ellipse model was also proposed.The three-dimensional contour of the pseudo-stem was calculated to obtain three types of phenotypic parameters:the long axis length,the short axis length,and the perimeter.Further,a synchronous trigger image acquisition mechanism was used to take pictures of pseudo-stems during measurement.It can be utilized for manual assessment of the growth status of the banana.Field experimental results showed that the three banana phenotypic parameters had a high correlation with the manual measurement results,and R^(2)is always more significant than 0.95,the total average measurement error and relative error were only 6.16 mm and 4.38%,respectively,both are within the acceptable agronomy range.In general,this method has good universality for plant stem detection,and the stem phenotypic parameters can be obtained by means of non-contact test,which is of great significance to the mechanized cultivation of the forest and fruit industry.展开更多
Retting has been employed to extract natural fibers from agricultural wastes as a biological and cost-effective approach for centuries.With its global abundance,banana pseudo-stem is a promising agro-waste for lignoce...Retting has been employed to extract natural fibers from agricultural wastes as a biological and cost-effective approach for centuries.With its global abundance,banana pseudo-stem is a promising agro-waste for lignocellulosic fiber extraction.In this study,fibers were extracted from the pseudo-stems after being pre-treated under four conditions using seawater at room temperature for up to 35 d Bacterial isolation from the fresh seawater sample and screening for ligninolytic ability were conducted.Bacterial load as well as laccase and manganese peroxidase enzyme activity profile assay during the retting duration were analyzed.Fourier transform infrared(FT-IR)and X-day diffraction(XRD)analyses were also examined for both pre-treated and untreated extracted fibers.The results shows that six out of the eight bacterial isolates had the ability to degrade lignin.The treatments(Raw stem+Raw seawater)and(Autoclaved stem+Raw seawater)recorded the highest viable bacterial load of 9.24×102 and 4.46×102 CFU,respectively,on the 14th day of the retting process.Additionally,the highest laccase and manganese peroxidase enzymes activity was recorded for(Raw stem+Raw seawater)and(Autoclaved stem+Raw seawater)treatments in the second to the third week.The FT-IR spectra of the pre-treated fibers revealed relative reductions in peaks attributed to polysaccharides and other amorphous substances for all retting conditions.The XRD diffractogram revealed that the crystallinity index(CI)of pre-treated fibers increased in all seawater retting treatment conditions.However,the CI for fibers pre-treated under enzymatic conditions were enhanced even after five weeks.Sequence analysis for selected bacterial isolates showed homology to sequences of Bacillus velezensis,Shewanella sp.L8–5,and Citrobacter amalonaticus and Bacillus subtilis j8 strain.From these findings,it was suggested that physical,biological,and chemical actions were collectively involved in the seawater retting process of banana pseudo-stems.展开更多
The upper and lower portions of Musa sapientum pseudo-stem sheaths were exposed in four types of treatments： pectin decomposing bacteria, magnesium oxide （MgO）, both pectin decomposing bacteria and MgO and control....The upper and lower portions of Musa sapientum pseudo-stem sheaths were exposed in four types of treatments： pectin decomposing bacteria, magnesium oxide （MgO）, both pectin decomposing bacteria and MgO and control. The fibre strength properties were tested using SIRIM Standard methods and colour changed using gray scale. The bioaugmentation of pectin decomposing bacteria together with MgO additions were found to enhance retting process and reduced retting periods 52% and yielded better strength on the fibres. The lower portion pseudo-stem sheaths gave the higher strength than upper portion. Colour changed showed that the bioaugmentation of pectin bacteria enhanced the degradation colouring compound in banana fibres.展开更多
基金Funded by the National Science Foundation of China(No.51263006)the Ministry of Education Ph D Advisor Class Special Fund(No.20134601110004)+2 种基金the Hainan Province of Key Projects(ZDXM20130086)the Hainan Province International Science and Technology Cooperation Specific(No.KJHZ2014-02)the Hainan Province Natural Science Foundation(No.314074)
文摘Banana pseudo-stem was liquefied in the mixture of polyhydric alcohols of polyethylene glycol(PEG400) and glycerol. Hydroxyl value of liquefied products ranged from 294.8 to 370.2 mg KOH/g and M_n was about 430. Liquefied products(LBPP) could be used as raw materials for polyurethane by reacting with 4, 4'-diphenylmethane diisocyanate(4, 4'-MDI) and PEG400 to synthesize liquefied product-based polyurethane(LBPP-PU) adhesive. To analyze in depth the creation of urethane linkage among LBPP, PEG400 and 4, 4'-MDI, factors which had effects on the residue content were all investigated. They were characterized by FT-IR and TG. The shear strength of LBPP-PU adhesive was improved when decreasing the percentage of the substitution of PEG400 by LBPP. The adhesive strength was obtained from T-peel of aspen/polyurethane adhesive joints, and the maximum lap shear strength(4.40 MPa) was obtained when 16.70% of LBPP was added to the LBPP & PEG400 system.
基金This research was financially supported by National Key Research and Development Project(2018YFD1000102,2019YFD1000200,2019YFD1000901)Guangdong Science and Technology Project(2019B030316007)+2 种基金special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science(R2018PY-QY004,R2017PY-QY001,R2017PY-JX002)Guangzhou national modern agricultural industry science and technology innovation center project(2018kczx06)National Banana Industry and Technology System Project(CARS-31-01).
文摘Banana(Musa spp.)is an ancient and popular fruit plant with highly nutritious fruit.The pseudo-stem of banana represents on average 75%of the total dry mass but its valorization as a nutritional and industrial by-product is limited.Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts.This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem.Hereby,we identified and quantified 373 metabolites from a diverse range of classes including,alkaloids,flavonoids,lipids,phenolic acids,amino acids and its derivatives,nucleotide and its derivatives,organic acids,lignans and coumarins,tannins,and terpene using the widely-targeted metabolomics approach.Banana pseudo-stem is enriched in metabolites for utilization in the food industry(L-lysine and L-tryptophan,L-glutamic acid,Phenylalanine,Palmitoleic acid,α-Linolenic acid,and Lauric acid,and Adenine)and pharmaceutical industry(Guanosine and Cimidahurinine,Bergapten,Coumarins,Procyanidin A2,Procyanidin B1,Procyanidin B3,Procyanidin B2,and Procyanidin B4,Asiatic acid).The metabolome of banana pseudo-stem with integration across multiomics data may provide the opportunity to exploit the rich metabolome of banana pseudo-stem for industrial and nutritional applications.
基金This work was financially supported by the Laboratory of Lingnan Modern Agriculture Project(Grant No.NT2021009)the National Key Research and Development Program of China(Grant No.2020YFD1000104)+2 种基金the China Agriculture Research System of MOF and MARA(Grant No.CARS-31-10)the Key-Areas Research and Development Program of Guangdong Province,China(Grant No.2019B020223002)the Department of Education Special Program of Guangdong Province,China(Grant No.2020KZDZX1036).
文摘The measurement of banana pseudo-stem phenotypic parameters is a critical way to evaluate the growth status of bananas,and it can provide essential data support for mechanized cultivation operations such as fertilization and pesticide application.Existing studies mainly measure the diameter of banana pseudo-stem as its phenotypic parameter.The banana pseudo-stem cross section was closer to an ellipse other than a standard circle,so the diameter parameter cannot adequately represent the phenotypic characteristics of the banana plant.In this study,an automatic measuring device for banana pseudo-stem phenotypic parameters was developed.The device,which integrates three different types of sensors:a laser ranging sensor,a rotary encoder,and a digital camera,were used to obtain the point cloud and image data of banana pseudo-stem.A K-means point clouds clustering algorithm based on Euclidean distance was proposed.The point cloud of banana pseudo-stem was identified and extracted.A three-dimensional reconstruction algorithm based on the ellipse model was also proposed.The three-dimensional contour of the pseudo-stem was calculated to obtain three types of phenotypic parameters:the long axis length,the short axis length,and the perimeter.Further,a synchronous trigger image acquisition mechanism was used to take pictures of pseudo-stems during measurement.It can be utilized for manual assessment of the growth status of the banana.Field experimental results showed that the three banana phenotypic parameters had a high correlation with the manual measurement results,and R^(2)is always more significant than 0.95,the total average measurement error and relative error were only 6.16 mm and 4.38%,respectively,both are within the acceptable agronomy range.In general,this method has good universality for plant stem detection,and the stem phenotypic parameters can be obtained by means of non-contact test,which is of great significance to the mechanized cultivation of the forest and fruit industry.
基金supported by the Science and Technology Development Fund(STDF)project(no.44049)and TICAD7 scholarship from the Egyptian and Japanese governments.We are also grateful to Professor Hiromi Nakanishi from the University of Tokyo,for the analyses with the PCR and TOYOBO for the sequencing.
文摘Retting has been employed to extract natural fibers from agricultural wastes as a biological and cost-effective approach for centuries.With its global abundance,banana pseudo-stem is a promising agro-waste for lignocellulosic fiber extraction.In this study,fibers were extracted from the pseudo-stems after being pre-treated under four conditions using seawater at room temperature for up to 35 d Bacterial isolation from the fresh seawater sample and screening for ligninolytic ability were conducted.Bacterial load as well as laccase and manganese peroxidase enzyme activity profile assay during the retting duration were analyzed.Fourier transform infrared(FT-IR)and X-day diffraction(XRD)analyses were also examined for both pre-treated and untreated extracted fibers.The results shows that six out of the eight bacterial isolates had the ability to degrade lignin.The treatments(Raw stem+Raw seawater)and(Autoclaved stem+Raw seawater)recorded the highest viable bacterial load of 9.24×102 and 4.46×102 CFU,respectively,on the 14th day of the retting process.Additionally,the highest laccase and manganese peroxidase enzymes activity was recorded for(Raw stem+Raw seawater)and(Autoclaved stem+Raw seawater)treatments in the second to the third week.The FT-IR spectra of the pre-treated fibers revealed relative reductions in peaks attributed to polysaccharides and other amorphous substances for all retting conditions.The XRD diffractogram revealed that the crystallinity index(CI)of pre-treated fibers increased in all seawater retting treatment conditions.However,the CI for fibers pre-treated under enzymatic conditions were enhanced even after five weeks.Sequence analysis for selected bacterial isolates showed homology to sequences of Bacillus velezensis,Shewanella sp.L8–5,and Citrobacter amalonaticus and Bacillus subtilis j8 strain.From these findings,it was suggested that physical,biological,and chemical actions were collectively involved in the seawater retting process of banana pseudo-stems.
文摘The upper and lower portions of Musa sapientum pseudo-stem sheaths were exposed in four types of treatments： pectin decomposing bacteria, magnesium oxide （MgO）, both pectin decomposing bacteria and MgO and control. The fibre strength properties were tested using SIRIM Standard methods and colour changed using gray scale. The bioaugmentation of pectin decomposing bacteria together with MgO additions were found to enhance retting process and reduced retting periods 52% and yielded better strength on the fibres. The lower portion pseudo-stem sheaths gave the higher strength than upper portion. Colour changed showed that the bioaugmentation of pectin bacteria enhanced the degradation colouring compound in banana fibres.
