Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusar...Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which exhibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees(Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation extract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse experiment using Highthroughput sequencing were analyzed, and the field experiment with grafted apple trees were used for further verification. Compared with the application of potato dextrose broth(PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field experiments. The abundance of Trichoderma,Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols,furans, and amino acids in root exudates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabolites with antifungal and growth-promoting potential. In addition, 6PS-2 spore suspension can also promote the growth of plants to a certain extent, and change the soil microbial community structure of rhizosphere soils. It is believed that T. virens 6PS-2 has the potential for the alleviation of apple replant disease(ARD) in China.展开更多
目的:分析支气管动脉化疗灌注与栓塞相结合的治疗方式对晚期肺癌患者(体能状态评分大于或等于2分(Performance Status Score≥2,PS≥2))的临床效果。方法:本研究纳入2020年5月至2021年10月期间到本院接受治疗的100例晚期肺癌患者(PS≥2...目的:分析支气管动脉化疗灌注与栓塞相结合的治疗方式对晚期肺癌患者(体能状态评分大于或等于2分(Performance Status Score≥2,PS≥2))的临床效果。方法:本研究纳入2020年5月至2021年10月期间到本院接受治疗的100例晚期肺癌患者(PS≥2分),按照治疗方法将其分为:对照组(n=42)采用静脉化疗,具体用药为吉西他滨(1000 mg•m^(-2),第1天和第8天)和顺铂(75 mg•m^(-2),第1天),每1个月为一个治疗周期;观察组(n=100)采用支气管动脉灌注化疗与栓塞术联合治疗,化疗药物剂量及用法与对照组一致,在完成化疗药物灌注后使用聚乙烯醇颗粒作为栓塞剂阻断肿瘤血供。两组患者均在治疗前及治疗4个月后采用化学发光免疫分析法测定血清CEA和CA125水平对比疗效、CEA和CA125水平,以及不良反应发生水平情况,1年、2年以及3年的生存率。结果:治疗6周后,相较于对照组,观察组疗效较高(P<0.05);在CEA、CA125水平方面,治疗4个月后两组CEA、CA125水平均显著降低且观察组水平均低于对照组(P<0.05);两组均经过为期3年的随访,观察组1年、2年以及3年的生存率均较对照组高(P<0.05);治疗4个月后,相较于对照组,观察组不良反应发生率较低(P<0.05)。结论:支气管动脉灌注化疗联合栓塞术对PS≥2分晚期肺癌患者具有更好的临床效果,能够在降低肿瘤标志物水平的同时提高生存率,并降低不良反应的发生风险,因此,值得在临床中推广应用。展开更多
基金supported by China Agriculture Research System of MOF and MARA (Grant No.CARS-27)Shandong Agricultural Major Applied Technology Innovation Project (Grant No.SD2019ZZ008)+5 种基金Qingchuang Science and Technology Support Project of Shandong Colleges and Universities (Grant No.2019KJF020)Natural Science Foundation of Shandong Province (Grant No.ZR2020MC131)the National Key Research and Development Program of China (Grant No.2020YFD1000201)Taishan Scholars Funded Project (Grant No.ts20190923)the National Natural Science Foundation of China (Grant No.32072510)the Fruit Innovation Team in Shandong Province,China (Grant No.SDAIT-06-07)。
文摘Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which exhibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees(Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation extract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse experiment using Highthroughput sequencing were analyzed, and the field experiment with grafted apple trees were used for further verification. Compared with the application of potato dextrose broth(PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field experiments. The abundance of Trichoderma,Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols,furans, and amino acids in root exudates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabolites with antifungal and growth-promoting potential. In addition, 6PS-2 spore suspension can also promote the growth of plants to a certain extent, and change the soil microbial community structure of rhizosphere soils. It is believed that T. virens 6PS-2 has the potential for the alleviation of apple replant disease(ARD) in China.
文摘目的:分析支气管动脉化疗灌注与栓塞相结合的治疗方式对晚期肺癌患者(体能状态评分大于或等于2分(Performance Status Score≥2,PS≥2))的临床效果。方法:本研究纳入2020年5月至2021年10月期间到本院接受治疗的100例晚期肺癌患者(PS≥2分),按照治疗方法将其分为:对照组(n=42)采用静脉化疗,具体用药为吉西他滨(1000 mg•m^(-2),第1天和第8天)和顺铂(75 mg•m^(-2),第1天),每1个月为一个治疗周期;观察组(n=100)采用支气管动脉灌注化疗与栓塞术联合治疗,化疗药物剂量及用法与对照组一致,在完成化疗药物灌注后使用聚乙烯醇颗粒作为栓塞剂阻断肿瘤血供。两组患者均在治疗前及治疗4个月后采用化学发光免疫分析法测定血清CEA和CA125水平对比疗效、CEA和CA125水平,以及不良反应发生水平情况,1年、2年以及3年的生存率。结果:治疗6周后,相较于对照组,观察组疗效较高(P<0.05);在CEA、CA125水平方面,治疗4个月后两组CEA、CA125水平均显著降低且观察组水平均低于对照组(P<0.05);两组均经过为期3年的随访,观察组1年、2年以及3年的生存率均较对照组高(P<0.05);治疗4个月后,相较于对照组,观察组不良反应发生率较低(P<0.05)。结论:支气管动脉灌注化疗联合栓塞术对PS≥2分晚期肺癌患者具有更好的临床效果,能够在降低肿瘤标志物水平的同时提高生存率,并降低不良反应的发生风险,因此,值得在临床中推广应用。
