The Botrytis is a worldwide necrotrophic pathogen,that causes gray mold disease and infects many economic vegetables and fruit.B.prunorum presented distinct biological characteristics compared to B.cinerea,including l...The Botrytis is a worldwide necrotrophic pathogen,that causes gray mold disease and infects many economic vegetables and fruit.B.prunorum presented distinct biological characteristics compared to B.cinerea,including lower pathogenicity.However,the low pathogenic mechanism of B.prunorum remains poorly understood,thereby limiting the development of effective strategies for disease control.In this study,the effects of B.prunorum strain R1V55#13 infection on the berries of two grape varieties were investigated.The reducing sugar content in berries inoculated with R1V55#13 increased by 6.5%in Thompson Seedless and 8.2%in Red Globe compared with water control.R1V55#13 decreased the titratable acid by 7.4%and 10.0%in the two grape varieties,respectively.The R1V55#13 strain had 421,290 mutation sites compared with the Bc05.10 strain,as indicated by the whole genome data,which might disrupt the gene function.Among these,ten predicted effector candidates exhibited 120 mutation sites in the R1V55#13 data.The mutation of BCIN_12g00630 potentially contributed to low pathogenicity of B.prunorum.The low pathogenicity of Botrytis was associated with mutations in gene coding cell wall degrading enzymes,laccases,and cytochrome P450s,such as BcPG1,BcPG2,BcLCC1,and BcLCC2.The infection of grape berries with high pathogenicity Botrytis strains led to higher levels of antifungal substances secreted,such as resveratrol and flavonoids.These findings provide further insights into the pathogenesis of Botrytis and facilitate the development of novel control mechanism,which help to reduce the reliance on chemical preservatives and enhance food safety.展开更多
基金supported by the Key R&D Project of Ningxia Hui Autonomous Region(No.2023BCF01023)the National Natural Science Foundation of China(No.32001991)the Key Research and Develop-ment Plan of Shaanxi Province(No.2023-YBNY-243).
文摘The Botrytis is a worldwide necrotrophic pathogen,that causes gray mold disease and infects many economic vegetables and fruit.B.prunorum presented distinct biological characteristics compared to B.cinerea,including lower pathogenicity.However,the low pathogenic mechanism of B.prunorum remains poorly understood,thereby limiting the development of effective strategies for disease control.In this study,the effects of B.prunorum strain R1V55#13 infection on the berries of two grape varieties were investigated.The reducing sugar content in berries inoculated with R1V55#13 increased by 6.5%in Thompson Seedless and 8.2%in Red Globe compared with water control.R1V55#13 decreased the titratable acid by 7.4%and 10.0%in the two grape varieties,respectively.The R1V55#13 strain had 421,290 mutation sites compared with the Bc05.10 strain,as indicated by the whole genome data,which might disrupt the gene function.Among these,ten predicted effector candidates exhibited 120 mutation sites in the R1V55#13 data.The mutation of BCIN_12g00630 potentially contributed to low pathogenicity of B.prunorum.The low pathogenicity of Botrytis was associated with mutations in gene coding cell wall degrading enzymes,laccases,and cytochrome P450s,such as BcPG1,BcPG2,BcLCC1,and BcLCC2.The infection of grape berries with high pathogenicity Botrytis strains led to higher levels of antifungal substances secreted,such as resveratrol and flavonoids.These findings provide further insights into the pathogenesis of Botrytis and facilitate the development of novel control mechanism,which help to reduce the reliance on chemical preservatives and enhance food safety.
