Extremely Low Frequency Magnetic Fields(ELF MF)has been considered as a“possible human carcinogen”by International Agency for Research on Cancer(IARC)while credible mechanisms of its carcinogenicity remain unknown.I...Extremely Low Frequency Magnetic Fields(ELF MF)has been considered as a“possible human carcinogen”by International Agency for Research on Cancer(IARC)while credible mechanisms of its carcinogenicity remain unknown.In this study,a proteomics approach was employed to investigate the changes of protein expression profile induced by ELF MF in human breast cancer cell line MCF7,in order to determine ELF MF-responsive proteins.MCF7 cells were exposed to 50 Hz,0.4 mT ELF MF for 24 h and the changes of protein profile were examined using two dimensional electrophoresis.Up to 6 spots have been statistically signifi-cantly altered(their expression levels were changed at least 5 fold up or down)compared with sham-exposed group.19 ones were only detected in exposure group while 19 ones were missing.Three proteins were identified by LC-IT Tandem MS as RNA binding protein regulatory subunit、Proteasome subunit beta type 7 precursor and Translationally Controlled Tumor Protein.Our finding showed that 50 Hz,0.4 mT ELF MF alternates the protein profile of MCF7 cell and may affect many physiological functions of normal cell and 2-DE coupled with MS is a promising ap-proach to elucidating cellular effects of electromagnetic fields.展开更多
Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a glob...Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span><span style="font-family:Verdana;">leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllu</span></i></span><i><span style="font-family:Verdana;">m </span></i><span style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein expression from the 2-DE gel map shows that 9 protein spots up-regulated and 41 protein spots were down-regulated with more than 2-fold differences (p < 0.05). This preliminary study on the PEP of MRSA treated with acetone extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leave may provide an insight into the antimicrobial mechanism, which could lead to the identification of target protein for future novel therapeutic development against MRSA infections.展开更多
基金This work was supported by the National Natural Science Foundat ion of China(Grant Nos.50137010&30170792)Zhejiang Provincial Key Projects for Science&Technology(Grant No.021106135)+1 种基金Zhejiang Provincial Natural Science Foundation(Grant No.301524)Key Projects of Health Bureau of Zhejiang Province(Grant No.2004ZD006).
文摘Extremely Low Frequency Magnetic Fields(ELF MF)has been considered as a“possible human carcinogen”by International Agency for Research on Cancer(IARC)while credible mechanisms of its carcinogenicity remain unknown.In this study,a proteomics approach was employed to investigate the changes of protein expression profile induced by ELF MF in human breast cancer cell line MCF7,in order to determine ELF MF-responsive proteins.MCF7 cells were exposed to 50 Hz,0.4 mT ELF MF for 24 h and the changes of protein profile were examined using two dimensional electrophoresis.Up to 6 spots have been statistically signifi-cantly altered(their expression levels were changed at least 5 fold up or down)compared with sham-exposed group.19 ones were only detected in exposure group while 19 ones were missing.Three proteins were identified by LC-IT Tandem MS as RNA binding protein regulatory subunit、Proteasome subunit beta type 7 precursor and Translationally Controlled Tumor Protein.Our finding showed that 50 Hz,0.4 mT ELF MF alternates the protein profile of MCF7 cell and may affect many physiological functions of normal cell and 2-DE coupled with MS is a promising ap-proach to elucidating cellular effects of electromagnetic fields.
文摘Methicillin-resistant </span><i><span style="font-family:Verdana;">Staphylococcus aureus </span></i><span style="font-family:Verdana;">(MRSA) infection is a global health concern that has caused severe health threats over the past decade. Leaves extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">has been proven previously as an anti MRSA agent. Proteomics provide a technique that used to analyze the differential of protein expression profile between untreated and treated MRSA with subinhibitory concentrations of acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span><span style="font-family:Verdana;">leaves. This study aims to determine the optimum parameter for analysis of protein expression profile using two-dimension gels electrophoresis (2-DE) for MRSA protein after treatment with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllu</span></i></span><i><span style="font-family:Verdana;">m </span></i><span style="font-family:Verdana;">leaves. Comparison of the Protein Expression Profile (PEP) between the untreated and treated MRSA was analyzed using PDQuest software. The optimum condition for MRSA protein treated with acetone extract from </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leaves to produce the best resolution with greater spot distribution was as follows: 100 μg volume of MRSA protein that loaded after passive rehydration then was run until reaching 25 kVrhs during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein expression from the 2-DE gel map shows that 9 protein spots up-regulated and 41 protein spots were down-regulated with more than 2-fold differences (p < 0.05). This preliminary study on the PEP of MRSA treated with acetone extract of </span><i><span style="font-family:Verdana;">C</span></i><span style="font-family:Verdana;">.</span><i><span style="font-family:Verdana;"> odontophyllum </span></i><span style="font-family:Verdana;">leave may provide an insight into the antimicrobial mechanism, which could lead to the identification of target protein for future novel therapeutic development against MRSA infections.
