转录后调控子Crc蛋白(Catabolite repression control protein)在防御假单胞菌(Pseudomonas protegens)H78中发挥着重要的代谢调控作用。运用RNA-seq(RNA sequencing)技术,针对H78野生型和H78Δcrc突变株开展比较转录组学研究,利用COG(C...转录后调控子Crc蛋白(Catabolite repression control protein)在防御假单胞菌(Pseudomonas protegens)H78中发挥着重要的代谢调控作用。运用RNA-seq(RNA sequencing)技术,针对H78野生型和H78Δcrc突变株开展比较转录组学研究,利用COG(Clusters of orthologous groups of proteins)、KEGG(Kyoto encyclopedia of genes and genomes)数据库对受到Crc调控的基因进行功能分类。转录组结果显示:Crc突变导致218个基因的转录子水平发生两倍以上的显著变化,其中105个基因表达上调,113个基因表达下调。Crc蛋白对碳、能量、脂肪及氨基酸代谢、抗生素合成等次级代谢具有全局调控作用,比如,脂质转运和代谢的基因簇(H78_00275~00283)表达显著下调约2.7~8.1倍,能量生产和转换的cyoA_2B_2C_2D_2E_1基因簇(H78_05248~05252)基因表达显著下调约3.4倍,调控氨基酸转运与代谢的H78_04826astAA_4DB基因簇(H78_04822~04826)基因表达显著上调约4倍,抗真菌剂藤黄绿菌素(pyoluteorin,Plt)的合成基因簇(pltLABCDEFG)、转运基因簇(pltIJKNOP)表达下调平均约3倍,而调控基因簇(pltR,pltM,pltZ)的表达平均下调不足2倍,整体上Plt合成受到Crc的显著正调控。Crc转录组学结果为进一步开展Plt等抗生素合成的分子调控网络与代谢工程研究奠定基础。展开更多
The rhizosphere bacterium Pseudomonas protegens FD6,which is associated with the production of antibiotics such as pyoluteorin(PLT)and 2,4-diacetylphloroglucinol(2,4-DAPG),has strong antagonistic effects on phytopatho...The rhizosphere bacterium Pseudomonas protegens FD6,which is associated with the production of antibiotics such as pyoluteorin(PLT)and 2,4-diacetylphloroglucinol(2,4-DAPG),has strong antagonistic effects on phytopathogens.Hfq is a conserved RNA chaperone involved in the regulation of stress tolerance,antibiotic production,and bacterial virulence.Here,we determined the regulatory effects of Hfq on biocontrol traits in P.protegens FD6.Mutation of hfq in FD6 reduced the growth rate,swimming and swarming ability,and production of proteases,siderophores,and hydrogen cyanide(HCN).Transmission electron microscopy revealed that disruption of hfq led to the loss of flagella.Furthermore,Hfq exerted a suppressive effect on biofilm formation and PLT production while not affecting antagonistic activity and control effect against tomato bacterial wilt.However,Hfq positively regulated the production of 2,4-DAPG and activated the phlD expression.Additionally,mutation of hfq abolished the function of the type VI secretion system(T6SS)due to a decrease in the expression of T6SS-related genes.Overall,these results suggest that Hfq plays a pleiotropic role in modulating the expression of biocontrol trait-related genes in P.protegens FD6.展开更多
文摘转录后调控子Crc蛋白(Catabolite repression control protein)在防御假单胞菌(Pseudomonas protegens)H78中发挥着重要的代谢调控作用。运用RNA-seq(RNA sequencing)技术,针对H78野生型和H78Δcrc突变株开展比较转录组学研究,利用COG(Clusters of orthologous groups of proteins)、KEGG(Kyoto encyclopedia of genes and genomes)数据库对受到Crc调控的基因进行功能分类。转录组结果显示:Crc突变导致218个基因的转录子水平发生两倍以上的显著变化,其中105个基因表达上调,113个基因表达下调。Crc蛋白对碳、能量、脂肪及氨基酸代谢、抗生素合成等次级代谢具有全局调控作用,比如,脂质转运和代谢的基因簇(H78_00275~00283)表达显著下调约2.7~8.1倍,能量生产和转换的cyoA_2B_2C_2D_2E_1基因簇(H78_05248~05252)基因表达显著下调约3.4倍,调控氨基酸转运与代谢的H78_04826astAA_4DB基因簇(H78_04822~04826)基因表达显著上调约4倍,抗真菌剂藤黄绿菌素(pyoluteorin,Plt)的合成基因簇(pltLABCDEFG)、转运基因簇(pltIJKNOP)表达下调平均约3倍,而调控基因簇(pltR,pltM,pltZ)的表达平均下调不足2倍,整体上Plt合成受到Crc的显著正调控。Crc转录组学结果为进一步开展Plt等抗生素合成的分子调控网络与代谢工程研究奠定基础。
基金supported by funds from the National Natural Science Foundation(32472628,32072471)the Science and Technology Program of Yangzhou City(YZ2023056)+1 种基金the Guangxi Key Laboratory of Agro-Environment and Agro-Product Safety Open Projects Fund(GKLAEAPS2023-04)the Jiangsu Province College Students Innovation and Entrepreneurship Training Program(2023117021Z).
文摘The rhizosphere bacterium Pseudomonas protegens FD6,which is associated with the production of antibiotics such as pyoluteorin(PLT)and 2,4-diacetylphloroglucinol(2,4-DAPG),has strong antagonistic effects on phytopathogens.Hfq is a conserved RNA chaperone involved in the regulation of stress tolerance,antibiotic production,and bacterial virulence.Here,we determined the regulatory effects of Hfq on biocontrol traits in P.protegens FD6.Mutation of hfq in FD6 reduced the growth rate,swimming and swarming ability,and production of proteases,siderophores,and hydrogen cyanide(HCN).Transmission electron microscopy revealed that disruption of hfq led to the loss of flagella.Furthermore,Hfq exerted a suppressive effect on biofilm formation and PLT production while not affecting antagonistic activity and control effect against tomato bacterial wilt.However,Hfq positively regulated the production of 2,4-DAPG and activated the phlD expression.Additionally,mutation of hfq abolished the function of the type VI secretion system(T6SS)due to a decrease in the expression of T6SS-related genes.Overall,these results suggest that Hfq plays a pleiotropic role in modulating the expression of biocontrol trait-related genes in P.protegens FD6.
