A novel electrogenerated chemiluminescence(ECL)aptasensor for highly sensitive detection of thrombin was developed on the basis of poly(pyrrole-co-pyrrole propylic acid)nanoparticles loaded with aptamer and ruthenium ...A novel electrogenerated chemiluminescence(ECL)aptasensor for highly sensitive detection of thrombin was developed on the basis of poly(pyrrole-co-pyrrole propylic acid)nanoparticles loaded with aptamer and ruthenium complex.Thrombin binding aptamers served as the molecular recognition elements and ruthenium bis(2,2'-bipyridine)(2,2'-bipyridine-4,4'-dicarboxylic acid)-ethylenediamine(Rul)was used as an ECL signal complex.Novel electroactive polymers poly(pyrrole-co-pyr-role propylic acid)nanoparticles(Ppy-pa NPs)were synthesized by a simple alcohol-assisted microemulsion polymerization.Rul-Ppy-pa NPs were synthesized by covalently coupling Rul with the Ppy-pa NPs.Ppy-pa NPs and Rul-Ppy-pa NPs were characterized using a fourier transform infrared spectrometer,super-conducting fourier digital NMR spectrometer,and trans-mission electron microscope.One ECL chemical sensor fabricated by immobilizing the Rul-Ppy-pa NPs on PIGE was developed for the determination of TprA with a high sensitivity and stability.The ECL aptasensor was fabricated by covalenfly coupling the thrombin binding aptamer-I(TBA-I)onto the surface of the paraffin-impregnated graphite electrode,which had been covalently modified with a monolayer of 4-aminobenzene sulfonic acid via electrochemical oxidations,for capturing thrombin onto the electrode and then the TBA-II labeled with Rul-Ppy-pa NPs was bound with epitope of thrombin.The ECL aptasensot showed an extremely low detection limit of 3.0×10^-16 mol/L for thrombin and a good selectivity.This work demonstrated that using Ppy-pa NPs as a carrier of ruthenium complex and molecular recognition element was a promising approach for the fabrication of ECL biosensor with high sensitivity.展开更多
High-pressure vapor-liquid equilibrium data for the binary systems of methyl propionate＋carbon dioxide and propyl propionate＋carbon dioxide were measured at pressure from 1.00 MPa to 12.00 MPa and temperature in the...High-pressure vapor-liquid equilibrium data for the binary systems of methyl propionate＋carbon dioxide and propyl propionate＋carbon dioxide were measured at pressure from 1.00 MPa to 12.00 MPa and temperature in the range from 313 K to 373 K. Experimental results were correlated with the Peng-Robinson equation of state with the two-parameter van der Waals mixing rule. At the same time, the Henry＇s coefficient, partial molar enthalpy change and partial molar entropy change of CO2 during dissolution at different temperature were also calculated.展开更多
We experimentally demonstrate the recognition of positional isomers of propyl alcohol vapor through nonlinear fluorescence induced by high-intensity femtosecond laser filaments in air. By measuring characteristic fluo...We experimentally demonstrate the recognition of positional isomers of propyl alcohol vapor through nonlinear fluorescence induced by high-intensity femtosecond laser filaments in air. By measuring characteristic fluorescence of n-propyl and isopropyl alcohol vapors produced by femtosecond filament excitation, it is found that they show identical spectra, that is, those from molecular bands of CH, C2, Nit, OH and CN, while the relative intensities are different. By comparing the ratios of the CH and C2 signals, the two propyl alcohol isomers are differentiated. The different signal intensities are ascribed to different ionization potentials of the two isomer molecules, leading to different production efficiencies of fluorescing fragments.展开更多
Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages. Methods: A screening model for COX inhibitors in vitro based on murine peri...Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages. Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term. Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1×10-5, 5×10-6 mol/L (P>0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1×10-6, 5×10-7, 1×10-7 mol/L (P<0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1×10-5,1×10-6 mol/L (P< 0. 05). Conclusion: Within the range of 1×10-5 to 1×10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages.展开更多
The adsorption mechanism of O-isopropyl-S-[2-(hydroxyimino) propyl] dithiocarbonate ester(IPXPO) to chalcopyrite was investigated by using contact angle, in-situ atomic force microscopy(in-situ AFM), cyclic voltammetr...The adsorption mechanism of O-isopropyl-S-[2-(hydroxyimino) propyl] dithiocarbonate ester(IPXPO) to chalcopyrite was investigated by using contact angle, in-situ atomic force microscopy(in-situ AFM), cyclic voltammetry(CV) and X-ray photoelectron spectroscopy(XPS). The results of contact angle and in-situ AFM demonstrated that IPXPO adsorbed on chalcopyrite increases surface hydrophobicity and roughness. It was found by CV experiments that a layer passive film was formed. The results of XPS spectra further revealed that the thiol S atom, oxime N atom, and O atom in the IPXPO molecule might react with copper atoms to form Cu-S, Cu-N, and Cu-O bonds, respectively. An artificial mixed minerals flotation test indicated that under the condition of pH=6.79 and IPXPO initial concentration 5×10^(-5)mol/L, the flotation recovery of chalcopyrite reached about 90%, while for pyrite only 25%, suggesting that IPXPO is an excellent collector for flotation separation and enrichment of chalcopyrite.展开更多
Thermosetting polyurethanes are widely used in various fields owing to their excellent elasticity,strength and solvent resistance.Three environmental friendly propyl gallate-based self-healing polyurethanes were prepa...Thermosetting polyurethanes are widely used in various fields owing to their excellent elasticity,strength and solvent resistance.Three environmental friendly propyl gallate-based self-healing polyurethanes were prepared from polyurethane prepolymers with varying isocyanate content.The thermal stabilities of the polyurethanes were tested using thermogravimetric analysis.Their self-healing and mechanical properties were analyzed using a universal testing machine and dynamic thermomechanical analysis.The polyurethanes were found with high self-healing ability and excellent mechanical properties due to the absence of phenolic carbamate.These qualities improved with increased isocyanate content and the prolonged selfhealing time.We found,therefore,that the propyl gallate-based polyurethane has potential for use in industrial applications as self-healing materials.展开更多
A highly selective synthesis of 2-aryl-1-arylmethyl-1H-1,3-benzimidazoles from the reaction of o-phenylenediamine and aromatic aldehydes in the presence of sulfuric acid{[3-(3-silicapropyl)sulfanyl]propyl}ester(SASPSP...A highly selective synthesis of 2-aryl-1-arylmethyl-1H-1,3-benzimidazoles from the reaction of o-phenylenediamine and aromatic aldehydes in the presence of sulfuric acid{[3-(3-silicapropyl)sulfanyl]propyl}ester(SASPSPE)in water and at 80℃in good to excellent yields.展开更多
The vapor-liquid equilibrium of binary mixtures of propyl acetate, butyl acetate and isobutyl acetate with meth- anol has been determined at a constant pressure of 0.6 MPa. Results have been modeled with the Peng-Robi...The vapor-liquid equilibrium of binary mixtures of propyl acetate, butyl acetate and isobutyl acetate with meth- anol has been determined at a constant pressure of 0.6 MPa. Results have been modeled with the Peng-Robinson equation, a traditional cubic equation of state widely employed in chemical industries, as well as with the perturbed-chain statistical associating fluid PC-SAFT theory of Gross-Sadowski. By correlation of the binary inter- action parameters of these equations, the measured vapor-liquid equilibrium data can be accurately predicted. Thus, this work shows that these models are able to represent the experimental data for systems with associating comoounds via hydrogen bonding.展开更多
In order to reduce the amount of volumetric shrinkage that occurs in dental composites as a result of curing,a new kind of dental matrix resin combining bisphenol-S-bis(3-meth acry late-2-hydroxy propyl)ether(BisS-GMA...In order to reduce the amount of volumetric shrinkage that occurs in dental composites as a result of curing,a new kind of dental matrix resin combining bisphenol-S-bis(3-meth acry late-2-hydroxy propyl)ether(BisS-GMA)with the expanding monomer unsaturated spiro orthoesters 2-methylene-1,4,6-trispiro[4,4]nonane(SOE)was prepared,with triethylene glycol dimethacrylate(TEGDMA)as diluent.CQ(camphorquinone)of 1wt%and DMAEMA(2-(dimethylamino)ethyl meth acrylate)of 2wt%were used as photoinitiation system to initiate the copolymerization of the matrix resins.The performance including volumetric shrinkage,degree of conversion and condition of the ring-opening reaction of SOE,as well as curing time and the tensile bond strength were investigated respectively by the dilatometer,Fourier transfer infrared,the universal testing machine,and so on.The ring-opening polymerization of SOE occurred.Meanwhile,the obtain copolymers were crosslinked.The matrix resin containing BisS-GMA and SOE showed a reduced amount of volumetric shrinkage at 1.52%,which is a promising strategy for obtaining a polymer with a low amount of volumetric shrinkage.Furthermore,the other properties were not compromised.展开更多
α-{3-[2-hydroxy-3-(N-methyl-N-hydroxyethylamino)propoxy]propyl}-α-butylpolydimethylsiloxanes III with various molecu-lar weights were prepared by epoxy addition ofα-[3-(2,3-epoxy-propoxy)propyl]-α-butylpolydimethy...α-{3-[2-hydroxy-3-(N-methyl-N-hydroxyethylamino)propoxy]propyl}-α-butylpolydimethylsiloxanes III with various molecu-lar weights were prepared by epoxy addition ofα-[3-(2,3-epoxy-propoxy)propyl]-α-butylpolydimethylsiloxanesⅡand N-methylmonoethanolamine.At each step,the outcome compounds were characterized through FT-IR and NMR spectra,the results showed that each step was successfully carried out and objective products were achieved.展开更多
The 3-morpholin-4-yl-propyl-ammine tetrafluoroborate（MPA^＋）（BF4^-）（1） and the 3-morpholin-4-ylpropyl-ammine perchlorate（MPA^＋）（ClO4^-）（2） were synthesized and separated as colorless block crystal.The（M...The 3-morpholin-4-yl-propyl-ammine tetrafluoroborate（MPA^＋）（BF4^-）（1） and the 3-morpholin-4-ylpropyl-ammine perchlorate（MPA^＋）（ClO4^-）（2） were synthesized and separated as colorless block crystal.The（MPA^＋）（BF4^-） undergoes a reversible phase transition at ca.347.3 K with a hysteresis of16.1 K.Dielectric measurement also can confirm the transition.The crystal structure determined at300 K（monoclinic,P21/c,a = 9.978（2）A,b = 7.5206（15）A,c = 15.058（3）A,b = 96.99（3）8,v = 1121.6（4）A^3,z = 4） and 373 K（triclinic,P-1,a = 10.021（7）A,b = 7.662（3）A,c = 7.920（4）A,a = 95.012（14）8,b = 101.67（4）8,g = 91.48（3）8,v = 592.7（6）A^3,z = 2） reveal that this compound has two phase.The most distinct is the BF4^- anion is disorder at 373 K,which is probably the driving force of the phase transition.DSC measurement,dielectric measurement and the crystal structure of the compound 2 did not change at 300 K and 100 K.展开更多
It was tried to develop the preparation method for microencapsulating the uycalyptus oil containing fine aqueous droplets by using the interfacial condensation reaction between hydroxyl propyl methyl cellulose and tan...It was tried to develop the preparation method for microencapsulating the uycalyptus oil containing fine aqueous droplets by using the interfacial condensation reaction between hydroxyl propyl methyl cellulose and tannic acid. Uycalyptus oil containing fine aqueous droplets was dispersed in the continuous water phase to form the (W/O)/W emulsion. Tannic acid and hydroxyl propyl methyl cellulose were dissolved in the inner aqueous droplets and in the outer continuous water phase, respectively. Tannic acid transferred through the oil phase from the inner water droplets to the interface between the oil phase and the continuous water phase and then, reacted with hydroxyl propyl methyl cellulose. In the experiment, the concentrations of hydroxyl propyl methyl cellulose and tannic acid were mainly changed stepwise. The uycalyptus oil containing the fine water droplets could be microencapsulated satisfactorily. It was found that the microcapsules were composed of the gelated hydroxyl propyl methyl cellulose film as the shell, the fine aqueous droplets as the first core and the oil droplet as the second core.展开更多
Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic Streptomyces SUK 08 as well as the main core structure fractionated from its crude extract.Methods: The activities of SUK 08 crude e...Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic Streptomyces SUK 08 as well as the main core structure fractionated from its crude extract.Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium lactate dehydrogenase assay and synchronization test against rodent malaria parasite Plasmodium berghei, instead of human malarial parasite Plasmodium falciparum. The cytotoxicity of the crude extract was determined by MTT assay. The crude extract was analyzed by thin-layer chromatography and gas chromatography–mass spectrophotometry.Results: The ethyl acetate crude extract showed very promising antimalarial activity with IC50 of 1.25 mg/m L. The synchronization tests showed that ethyl acetate extraction could inhibit all stages of the Plasmodium life cycle, but it was most effective at the Plasmodium ring stage. On the basis of a MTT assay on Chang Liver cells, ethyl acetate and ethanol demonstrated IC50 values of >1.0 mg/m L. The IC50 of parasitemia at 5% and30% for this extract was lower than chloroquine. Thin-layer chromatography, with 1: 9 ratio of ethyl acetate: hexane, was used to isolate several distinct compounds. Based on gas chromatography–mass spectrophotometry analysis, three core structures were identified as cyclohexane, butyl propyl ester, and 2,3-heptanedione. Structurally, these compounds were similar to currently available antimalarial drugs.Conclusions: The results suggest that compounds isolated from Streptomyces SUK 08 are viable antimalarial drug candidates that require further investigations.展开更多
Objective:The objective of the present study was to develop floating microspheres of Cefpodoxime Proxetil in order to achieve an extended retention in the upper GIT,which may result in enhanced absorption and there by...Objective:The objective of the present study was to develop floating microspheres of Cefpodoxime Proxetil in order to achieve an extended retention in the upper GIT,which may result in enhanced absorption and there by improved bioavailability.Methods:The microspheres were prepared by non - aqueous solvent evaporation method using polymers such as Hydroxyl Propyl Methyl Cellulose（HPMC K15M）,Ethyl Cellulose（EC） in different ratios,and Cefpodoxime Proxetil contain in each formulation.In vitro drug release were performed by USP apparatus type I andthe microspheres were characterized by calculating percentage yield,particle size analysis, buoyancy percentage,drug entrapment efficiencyand in vitro drug release studies.Results:The result showed microspheres yield were 50.50%-72.21%,particle size were distributed between75-600μm,drug entrapment efficiency were 14.1%-28.2%,buoyancy percentage were 70.10%-88.25%.Conclusion: Cefpodoxime Proxetil floating microspheres,at the lower polymer to drug ratio,there was a significant drug release. The better drug release profile was seen with FA<sub>2</sub> with ratio of drug polymer（1:2）.展开更多
The fullerene C-60 and phthatocyanine co-grafted poly (epoxy propyl carbazole) (C-60-CuPC-PEPC) was synthesized by Friedel-Crafts reaction. It was found that C-60-CuPc-PEPC had good UV-Vis absorption property, whose a...The fullerene C-60 and phthatocyanine co-grafted poly (epoxy propyl carbazole) (C-60-CuPC-PEPC) was synthesized by Friedel-Crafts reaction. It was found that C-60-CuPc-PEPC had good UV-Vis absorption property, whose active spectral range was expanded from UV to visible spectrum region, and exhibited better photoconductivity than CuPc-PEPC and PEPC. These improvements could be attributed to the interaction between C-60, phthalocyanine and PEPC.展开更多
Rasagiline,a monoamine oxidase-B inhibitor,and bis（propyl）-cognitin（B3C）,a novel dimer are reported to be neuroprotective.Herein,the synergistical neuroprotection produced by rasagiline and B3 C was investigated i...Rasagiline,a monoamine oxidase-B inhibitor,and bis（propyl）-cognitin（B3C）,a novel dimer are reported to be neuroprotective.Herein,the synergistical neuroprotection produced by rasagiline and B3 C was investigated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced mice of Parkinsonism.By using neurobehavioural tests,high-performance liquid chromatography and western blot assay,we showed that B3 C at 0.3 mg/kg,rasagiline at 0.02 mg/kg,as well as co-treatment with B3 C and rasagiline prevented MPTP-induced behavioural abnormities,increased the concentrations of dopamine and its metabolites in the striatum,and up-regulated the expression of tyrosine hydroxylase in the substantia nigra.However,the neuroprotective effects of co-treatment were not significantly improved when compared with those of B3 C or rasagiline alone.Collectively,we have demonstrated that B3 C at 0.3 mg/kg and rasagline at 0.02 mg/kg could not produce synergistic neuroprotective effects.展开更多
Poly N-isopropyl acrylamide (abbreviated as PNIPA) as a kind of thermally sensitive hydrogel is utilized to concentrate Bovin Serum Albumin (BSA) solution. In order to decrease its surface adsorption to BSA in aqueous...Poly N-isopropyl acrylamide (abbreviated as PNIPA) as a kind of thermally sensitive hydrogel is utilized to concentrate Bovin Serum Albumin (BSA) solution. In order to decrease its surface adsorption to BSA in aqueous solution, surface layer grafting of the gels by radiation technology was carried out. The results showed that hydroxyl propyl methacrylate (HPMA) grained gel exhibited a low level of BSA adsorption and still kept the original thermally sensitive properties of PNIPA hydrogels.展开更多
The polysiloxane containing propylene carbonate side group and several lithium poly-meric salts were synthesized. The structure were confirmed by IR, NMR and XPS. Theblending systems of polysiloxane containing propyle...The polysiloxane containing propylene carbonate side group and several lithium poly-meric salts were synthesized. The structure were confirmed by IR, NMR and XPS. Theblending systems of polysiloxane containing propylene carbonate group with different lithiumpolymeric salts were studied by ion conductivity XPS and DSC. Different lithium poly-meric salts in the blending system lead to conductivity arranged in the following sequence:poly(lithium ethylenebenzene sulfonate methylsiloxane)>poly(lithium propionate methyl-siloxane)>poly(lithium propylsulfonate methylsiloxane)>poly(lithium styrenesulfonate).In the blending system the best single ion conductivity was close to 10^(-5) Scm^(-1) at roomtemperature. XPS showed that at low lithium salt concentration the conductivity increasedwith the increasing content of lithium salt, in consequence of the increase of free ion andsolvent separated ion pair. At high lithium salt concentration the free ion was absent andthe solvent-separated ion pair functioned as carrier.展开更多
Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature s...Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA (Bovin serum albumin) dilute aqueous solution by hydroxylpropyl methacrylate (HPMA) copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.展开更多
基金supported by the National Natural Science Foundation of China(21027007,20975065&20775046)
文摘A novel electrogenerated chemiluminescence(ECL)aptasensor for highly sensitive detection of thrombin was developed on the basis of poly(pyrrole-co-pyrrole propylic acid)nanoparticles loaded with aptamer and ruthenium complex.Thrombin binding aptamers served as the molecular recognition elements and ruthenium bis(2,2'-bipyridine)(2,2'-bipyridine-4,4'-dicarboxylic acid)-ethylenediamine(Rul)was used as an ECL signal complex.Novel electroactive polymers poly(pyrrole-co-pyr-role propylic acid)nanoparticles(Ppy-pa NPs)were synthesized by a simple alcohol-assisted microemulsion polymerization.Rul-Ppy-pa NPs were synthesized by covalently coupling Rul with the Ppy-pa NPs.Ppy-pa NPs and Rul-Ppy-pa NPs were characterized using a fourier transform infrared spectrometer,super-conducting fourier digital NMR spectrometer,and trans-mission electron microscope.One ECL chemical sensor fabricated by immobilizing the Rul-Ppy-pa NPs on PIGE was developed for the determination of TprA with a high sensitivity and stability.The ECL aptasensor was fabricated by covalenfly coupling the thrombin binding aptamer-I(TBA-I)onto the surface of the paraffin-impregnated graphite electrode,which had been covalently modified with a monolayer of 4-aminobenzene sulfonic acid via electrochemical oxidations,for capturing thrombin onto the electrode and then the TBA-II labeled with Rul-Ppy-pa NPs was bound with epitope of thrombin.The ECL aptasensot showed an extremely low detection limit of 3.0×10^-16 mol/L for thrombin and a good selectivity.This work demonstrated that using Ppy-pa NPs as a carrier of ruthenium complex and molecular recognition element was a promising approach for the fabrication of ECL biosensor with high sensitivity.
文摘High-pressure vapor-liquid equilibrium data for the binary systems of methyl propionate＋carbon dioxide and propyl propionate＋carbon dioxide were measured at pressure from 1.00 MPa to 12.00 MPa and temperature in the range from 313 K to 373 K. Experimental results were correlated with the Peng-Robinson equation of state with the two-parameter van der Waals mixing rule. At the same time, the Henry＇s coefficient, partial molar enthalpy change and partial molar entropy change of CO2 during dissolution at different temperature were also calculated.
基金Supported by the National Natural Science Foundation of China under Grant Nos 61427816 and 61235003the Research Fund for the Doctoral Program of Higher Education of China under Grant No 20130061110047the Open Fund of the State Key Laboratory of High Field Laser Physics
文摘We experimentally demonstrate the recognition of positional isomers of propyl alcohol vapor through nonlinear fluorescence induced by high-intensity femtosecond laser filaments in air. By measuring characteristic fluorescence of n-propyl and isopropyl alcohol vapors produced by femtosecond filament excitation, it is found that they show identical spectra, that is, those from molecular bands of CH, C2, Nit, OH and CN, while the relative intensities are different. By comparing the ratios of the CH and C2 signals, the two propyl alcohol isomers are differentiated. The different signal intensities are ascribed to different ionization potentials of the two isomer molecules, leading to different production efficiencies of fluorescing fragments.
文摘Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages. Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term. Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1×10-5, 5×10-6 mol/L (P>0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1×10-6, 5×10-7, 1×10-7 mol/L (P<0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1×10-5,1×10-6 mol/L (P< 0. 05). Conclusion: Within the range of 1×10-5 to 1×10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages.
基金Projects(22108114, 5180031184) supported by the National Natural Science Foundation of China。
文摘The adsorption mechanism of O-isopropyl-S-[2-(hydroxyimino) propyl] dithiocarbonate ester(IPXPO) to chalcopyrite was investigated by using contact angle, in-situ atomic force microscopy(in-situ AFM), cyclic voltammetry(CV) and X-ray photoelectron spectroscopy(XPS). The results of contact angle and in-situ AFM demonstrated that IPXPO adsorbed on chalcopyrite increases surface hydrophobicity and roughness. It was found by CV experiments that a layer passive film was formed. The results of XPS spectra further revealed that the thiol S atom, oxime N atom, and O atom in the IPXPO molecule might react with copper atoms to form Cu-S, Cu-N, and Cu-O bonds, respectively. An artificial mixed minerals flotation test indicated that under the condition of pH=6.79 and IPXPO initial concentration 5×10^(-5)mol/L, the flotation recovery of chalcopyrite reached about 90%, while for pyrite only 25%, suggesting that IPXPO is an excellent collector for flotation separation and enrichment of chalcopyrite.
基金supported by the National Natural Science Foundation of China.(Grand No.31570563)Jiangsu Province Biomass Energy and Materials Laboratory,China(Grant No.JSBEM-S-201807)Fundamental Research Funds of Chinese academy of forestry(CAFYBB2017MB017).
文摘Thermosetting polyurethanes are widely used in various fields owing to their excellent elasticity,strength and solvent resistance.Three environmental friendly propyl gallate-based self-healing polyurethanes were prepared from polyurethane prepolymers with varying isocyanate content.The thermal stabilities of the polyurethanes were tested using thermogravimetric analysis.Their self-healing and mechanical properties were analyzed using a universal testing machine and dynamic thermomechanical analysis.The polyurethanes were found with high self-healing ability and excellent mechanical properties due to the absence of phenolic carbamate.These qualities improved with increased isocyanate content and the prolonged selfhealing time.We found,therefore,that the propyl gallate-based polyurethane has potential for use in industrial applications as self-healing materials.
文摘A highly selective synthesis of 2-aryl-1-arylmethyl-1H-1,3-benzimidazoles from the reaction of o-phenylenediamine and aromatic aldehydes in the presence of sulfuric acid{[3-(3-silicapropyl)sulfanyl]propyl}ester(SASPSPE)in water and at 80℃in good to excellent yields.
文摘The vapor-liquid equilibrium of binary mixtures of propyl acetate, butyl acetate and isobutyl acetate with meth- anol has been determined at a constant pressure of 0.6 MPa. Results have been modeled with the Peng-Robinson equation, a traditional cubic equation of state widely employed in chemical industries, as well as with the perturbed-chain statistical associating fluid PC-SAFT theory of Gross-Sadowski. By correlation of the binary inter- action parameters of these equations, the measured vapor-liquid equilibrium data can be accurately predicted. Thus, this work shows that these models are able to represent the experimental data for systems with associating comoounds via hydrogen bonding.
基金Funded by the National Natural Science Foundation of China(No.50673065)
文摘In order to reduce the amount of volumetric shrinkage that occurs in dental composites as a result of curing,a new kind of dental matrix resin combining bisphenol-S-bis(3-meth acry late-2-hydroxy propyl)ether(BisS-GMA)with the expanding monomer unsaturated spiro orthoesters 2-methylene-1,4,6-trispiro[4,4]nonane(SOE)was prepared,with triethylene glycol dimethacrylate(TEGDMA)as diluent.CQ(camphorquinone)of 1wt%and DMAEMA(2-(dimethylamino)ethyl meth acrylate)of 2wt%were used as photoinitiation system to initiate the copolymerization of the matrix resins.The performance including volumetric shrinkage,degree of conversion and condition of the ring-opening reaction of SOE,as well as curing time and the tensile bond strength were investigated respectively by the dilatometer,Fourier transfer infrared,the universal testing machine,and so on.The ring-opening polymerization of SOE occurred.Meanwhile,the obtain copolymers were crosslinked.The matrix resin containing BisS-GMA and SOE showed a reduced amount of volumetric shrinkage at 1.52%,which is a promising strategy for obtaining a polymer with a low amount of volumetric shrinkage.Furthermore,the other properties were not compromised.
基金the Natural Science Foundation of Shandong Province(No.Y2006B22).
文摘α-{3-[2-hydroxy-3-(N-methyl-N-hydroxyethylamino)propoxy]propyl}-α-butylpolydimethylsiloxanes III with various molecu-lar weights were prepared by epoxy addition ofα-[3-(2,3-epoxy-propoxy)propyl]-α-butylpolydimethylsiloxanesⅡand N-methylmonoethanolamine.At each step,the outcome compounds were characterized through FT-IR and NMR spectra,the results showed that each step was successfully carried out and objective products were achieved.
基金supported by the Project 973 (No.2014CB848800)National Natural Science Foundation of China (Nos.21471032,21422101 and 21301029)+2 种基金Jiangsu Province NSF (Nos.BK20140056 and BK20130600)Program for NCETPh.D.Programs Foundation of Ministry of Education of China (No.20130092120013)
文摘The 3-morpholin-4-yl-propyl-ammine tetrafluoroborate（MPA^＋）（BF4^-）（1） and the 3-morpholin-4-ylpropyl-ammine perchlorate（MPA^＋）（ClO4^-）（2） were synthesized and separated as colorless block crystal.The（MPA^＋）（BF4^-） undergoes a reversible phase transition at ca.347.3 K with a hysteresis of16.1 K.Dielectric measurement also can confirm the transition.The crystal structure determined at300 K（monoclinic,P21/c,a = 9.978（2）A,b = 7.5206（15）A,c = 15.058（3）A,b = 96.99（3）8,v = 1121.6（4）A^3,z = 4） and 373 K（triclinic,P-1,a = 10.021（7）A,b = 7.662（3）A,c = 7.920（4）A,a = 95.012（14）8,b = 101.67（4）8,g = 91.48（3）8,v = 592.7（6）A^3,z = 2） reveal that this compound has two phase.The most distinct is the BF4^- anion is disorder at 373 K,which is probably the driving force of the phase transition.DSC measurement,dielectric measurement and the crystal structure of the compound 2 did not change at 300 K and 100 K.
文摘It was tried to develop the preparation method for microencapsulating the uycalyptus oil containing fine aqueous droplets by using the interfacial condensation reaction between hydroxyl propyl methyl cellulose and tannic acid. Uycalyptus oil containing fine aqueous droplets was dispersed in the continuous water phase to form the (W/O)/W emulsion. Tannic acid and hydroxyl propyl methyl cellulose were dissolved in the inner aqueous droplets and in the outer continuous water phase, respectively. Tannic acid transferred through the oil phase from the inner water droplets to the interface between the oil phase and the continuous water phase and then, reacted with hydroxyl propyl methyl cellulose. In the experiment, the concentrations of hydroxyl propyl methyl cellulose and tannic acid were mainly changed stepwise. The uycalyptus oil containing the fine water droplets could be microencapsulated satisfactorily. It was found that the microcapsules were composed of the gelated hydroxyl propyl methyl cellulose film as the shell, the fine aqueous droplets as the first core and the oil droplet as the second core.
基金financial assistance provided by Malaysia's Ministry of Higher Education (Grant number: FRGS/2/2010/SG/ UKM/01/9)the Universiti Kebangsaan Malaysia (Grant number: UKM_GUP-TKP-08-22-074) for making this study possible
文摘Objective: To determine ex vivo antimalarial activity and cytotoxicity of endophytic Streptomyces SUK 08 as well as the main core structure fractionated from its crude extract.Methods: The activities of SUK 08 crude extract were evaluated by using the Plasmodium lactate dehydrogenase assay and synchronization test against rodent malaria parasite Plasmodium berghei, instead of human malarial parasite Plasmodium falciparum. The cytotoxicity of the crude extract was determined by MTT assay. The crude extract was analyzed by thin-layer chromatography and gas chromatography–mass spectrophotometry.Results: The ethyl acetate crude extract showed very promising antimalarial activity with IC50 of 1.25 mg/m L. The synchronization tests showed that ethyl acetate extraction could inhibit all stages of the Plasmodium life cycle, but it was most effective at the Plasmodium ring stage. On the basis of a MTT assay on Chang Liver cells, ethyl acetate and ethanol demonstrated IC50 values of >1.0 mg/m L. The IC50 of parasitemia at 5% and30% for this extract was lower than chloroquine. Thin-layer chromatography, with 1: 9 ratio of ethyl acetate: hexane, was used to isolate several distinct compounds. Based on gas chromatography–mass spectrophotometry analysis, three core structures were identified as cyclohexane, butyl propyl ester, and 2,3-heptanedione. Structurally, these compounds were similar to currently available antimalarial drugs.Conclusions: The results suggest that compounds isolated from Streptomyces SUK 08 are viable antimalarial drug candidates that require further investigations.
文摘Objective:The objective of the present study was to develop floating microspheres of Cefpodoxime Proxetil in order to achieve an extended retention in the upper GIT,which may result in enhanced absorption and there by improved bioavailability.Methods:The microspheres were prepared by non - aqueous solvent evaporation method using polymers such as Hydroxyl Propyl Methyl Cellulose（HPMC K15M）,Ethyl Cellulose（EC） in different ratios,and Cefpodoxime Proxetil contain in each formulation.In vitro drug release were performed by USP apparatus type I andthe microspheres were characterized by calculating percentage yield,particle size analysis, buoyancy percentage,drug entrapment efficiencyand in vitro drug release studies.Results:The result showed microspheres yield were 50.50%-72.21%,particle size were distributed between75-600μm,drug entrapment efficiency were 14.1%-28.2%,buoyancy percentage were 70.10%-88.25%.Conclusion: Cefpodoxime Proxetil floating microspheres,at the lower polymer to drug ratio,there was a significant drug release. The better drug release profile was seen with FA<sub>2</sub> with ratio of drug polymer（1:2）.
文摘The fullerene C-60 and phthatocyanine co-grafted poly (epoxy propyl carbazole) (C-60-CuPC-PEPC) was synthesized by Friedel-Crafts reaction. It was found that C-60-CuPc-PEPC had good UV-Vis absorption property, whose active spectral range was expanded from UV to visible spectrum region, and exhibited better photoconductivity than CuPc-PEPC and PEPC. These improvements could be attributed to the interaction between C-60, phthalocyanine and PEPC.
基金supported by grants from the Natural Science Foundation of Guangdong Province of China,No.2015A030313317a grant from the Science and Technology Program of Guangzhou City of China,No.2014J4100097+3 种基金partially by a grant from the Science and Technology Development Fund(FDCT) of Macao Special Administrative Region,No.134/2014/A3a grant from the Research Committee of University of Macao,No.MYRG139(Y1-L4)-ICMS12-LMY and MYRG2015-00214-ICMS-QRCMgrants from the Research Grants Council of Hong Kong Special Administrative Region of China,No.561011,15101014the Hong Kong Polytechnic University of China,No.G-SB10,G-UC15 and G-YBGQ
文摘Rasagiline,a monoamine oxidase-B inhibitor,and bis（propyl）-cognitin（B3C）,a novel dimer are reported to be neuroprotective.Herein,the synergistical neuroprotection produced by rasagiline and B3 C was investigated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced mice of Parkinsonism.By using neurobehavioural tests,high-performance liquid chromatography and western blot assay,we showed that B3 C at 0.3 mg/kg,rasagiline at 0.02 mg/kg,as well as co-treatment with B3 C and rasagiline prevented MPTP-induced behavioural abnormities,increased the concentrations of dopamine and its metabolites in the striatum,and up-regulated the expression of tyrosine hydroxylase in the substantia nigra.However,the neuroprotective effects of co-treatment were not significantly improved when compared with those of B3 C or rasagiline alone.Collectively,we have demonstrated that B3 C at 0.3 mg/kg and rasagline at 0.02 mg/kg could not produce synergistic neuroprotective effects.
基金This project is sponsored by the National Natural Science Foundation of China.
文摘Poly N-isopropyl acrylamide (abbreviated as PNIPA) as a kind of thermally sensitive hydrogel is utilized to concentrate Bovin Serum Albumin (BSA) solution. In order to decrease its surface adsorption to BSA in aqueous solution, surface layer grafting of the gels by radiation technology was carried out. The results showed that hydroxyl propyl methacrylate (HPMA) grained gel exhibited a low level of BSA adsorption and still kept the original thermally sensitive properties of PNIPA hydrogels.
基金This work was supported by the National Natural Science Foundation of China.
文摘The polysiloxane containing propylene carbonate side group and several lithium poly-meric salts were synthesized. The structure were confirmed by IR, NMR and XPS. Theblending systems of polysiloxane containing propylene carbonate group with different lithiumpolymeric salts were studied by ion conductivity XPS and DSC. Different lithium poly-meric salts in the blending system lead to conductivity arranged in the following sequence:poly(lithium ethylenebenzene sulfonate methylsiloxane)>poly(lithium propionate methyl-siloxane)>poly(lithium propylsulfonate methylsiloxane)>poly(lithium styrenesulfonate).In the blending system the best single ion conductivity was close to 10^(-5) Scm^(-1) at roomtemperature. XPS showed that at low lithium salt concentration the conductivity increasedwith the increasing content of lithium salt, in consequence of the increase of free ion andsolvent separated ion pair. At high lithium salt concentration the free ion was absent andthe solvent-separated ion pair functioned as carrier.
基金This project sponsored by the National Natural Science Foundation of China.
文摘Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA (Bovin serum albumin) dilute aqueous solution by hydroxylpropyl methacrylate (HPMA) copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.
