Primary cilia function as critical sensory organelles that mediate multiple signaling pathways,including the Hedgehog(Hh)pathway,which is essential for organ patterning and morphogenesis.Disruptions in Hh signaling ha...Primary cilia function as critical sensory organelles that mediate multiple signaling pathways,including the Hedgehog(Hh)pathway,which is essential for organ patterning and morphogenesis.Disruptions in Hh signaling have been implicated in supernumerary tooth formation and molar fusion in mutant mice.Cilk1,a highly conserved serine/threonine-protein kinase localized within primary cilia,plays a critical role in ciliary transport.Loss of Cilk1 results in severe ciliopathy phenotypes,including polydactyly,edema,and cleft palate.However,the role of Cilk1 in tooth development remains unexplored.In this study,we investigated the role of Cilk1 in tooth development.Cilk1 was found to be expressed in both the epithelial and mesenchymal compartments of developing molars.Cilk1 deficiency resulted in altered ciliary dynamics,characterized by reduced frequency and increased length,accompanied by downregulation of Hh target genes,such as Ptch1 and Sostdc1,leading to the formation of diastemal supernumerary teeth.Furthermore,in Cilk1^(-/-);PCS1–MRCS1^(△/△)mice,which exhibit a compounded suppression of Hh signaling,we uncovered a novel phenomenon:diastemal supernumerary teeth can be larger than first molars.Based on these findings,we propose a progressive model linking Hh signaling levels to sequential changes in tooth patterning:initially inducing diastemal supernumerary teeth,then enlarging them,and ultimately leading to molar fusion.This study reveals a previously unrecognized role of Cilk1 in controlling tooth morphology via Hh signaling and highlights how Hh signaling levels shape tooth patterning in a gradient-dependent manner.展开更多
A research project on interaction between agroecosystems and global change has been accepted as a Category Ⅰ contribution to IGBP-GCTE (International Geosphere-Biosphere Programme-Global Change & Terrestrial Ecos...A research project on interaction between agroecosystems and global change has been accepted as a Category Ⅰ contribution to IGBP-GCTE (International Geosphere-Biosphere Programme-Global Change & Terrestrial Ecosystems) Core Research, the highest-level support GCTE has offered to an individual research project so far. This was confirmed by Prof. Noble, GCTE Chairman, in a letter to Prof. Peng Shaolin, Principal Investigator of展开更多
Oral squamous cell carcinoma(OSCC),a major subgroup of head and neck squamous cell carcinoma(HNSCC),is an aggressive disease that preferentially spreads to cervical lymph nodes.Positive lymph node status is an importa...Oral squamous cell carcinoma(OSCC),a major subgroup of head and neck squamous cell carcinoma(HNSCC),is an aggressive disease that preferentially spreads to cervical lymph nodes.Positive lymph node status is an important predictor of survival in OSCC[1-3].Hence,a better understanding of the molecular mechanisms underlying oral cancer metastasis and the identification of therapeutic vulnerabilities are needed to prevent and treat metastatic disease.展开更多
The year 2017 is the first year for the U.S.Trump Administration,is the election year to determine the fate of many European countries,is the year for changing situation in the Middle East,and is the year for the Chin...The year 2017 is the first year for the U.S.Trump Administration,is the election year to determine the fate of many European countries,is the year for changing situation in the Middle East,and is the year for the Chinese diplomacy to open a new chapter.What are the展开更多
基金supported by the National Research Foundation of Korea(NRF)grants funded by the Korean government(MSIT)(NRF-2020R1A2C2005790,NRF2023R1A2C1007510,RS-2023-00269830,RS-2024-00438542)。
文摘Primary cilia function as critical sensory organelles that mediate multiple signaling pathways,including the Hedgehog(Hh)pathway,which is essential for organ patterning and morphogenesis.Disruptions in Hh signaling have been implicated in supernumerary tooth formation and molar fusion in mutant mice.Cilk1,a highly conserved serine/threonine-protein kinase localized within primary cilia,plays a critical role in ciliary transport.Loss of Cilk1 results in severe ciliopathy phenotypes,including polydactyly,edema,and cleft palate.However,the role of Cilk1 in tooth development remains unexplored.In this study,we investigated the role of Cilk1 in tooth development.Cilk1 was found to be expressed in both the epithelial and mesenchymal compartments of developing molars.Cilk1 deficiency resulted in altered ciliary dynamics,characterized by reduced frequency and increased length,accompanied by downregulation of Hh target genes,such as Ptch1 and Sostdc1,leading to the formation of diastemal supernumerary teeth.Furthermore,in Cilk1^(-/-);PCS1–MRCS1^(△/△)mice,which exhibit a compounded suppression of Hh signaling,we uncovered a novel phenomenon:diastemal supernumerary teeth can be larger than first molars.Based on these findings,we propose a progressive model linking Hh signaling levels to sequential changes in tooth patterning:initially inducing diastemal supernumerary teeth,then enlarging them,and ultimately leading to molar fusion.This study reveals a previously unrecognized role of Cilk1 in controlling tooth morphology via Hh signaling and highlights how Hh signaling levels shape tooth patterning in a gradient-dependent manner.
文摘A research project on interaction between agroecosystems and global change has been accepted as a Category Ⅰ contribution to IGBP-GCTE (International Geosphere-Biosphere Programme-Global Change & Terrestrial Ecosystems) Core Research, the highest-level support GCTE has offered to an individual research project so far. This was confirmed by Prof. Noble, GCTE Chairman, in a letter to Prof. Peng Shaolin, Principal Investigator of
基金supported by intramural funding from the Medical Faculty(Wilhelm-Roux program,FKZ32/19).
文摘Oral squamous cell carcinoma(OSCC),a major subgroup of head and neck squamous cell carcinoma(HNSCC),is an aggressive disease that preferentially spreads to cervical lymph nodes.Positive lymph node status is an important predictor of survival in OSCC[1-3].Hence,a better understanding of the molecular mechanisms underlying oral cancer metastasis and the identification of therapeutic vulnerabilities are needed to prevent and treat metastatic disease.
文摘The year 2017 is the first year for the U.S.Trump Administration,is the election year to determine the fate of many European countries,is the year for changing situation in the Middle East,and is the year for the Chinese diplomacy to open a new chapter.What are the
