Rapid and sensitive recognition of herbal pieces according to different concocted processing is crucial to quality control and pharmaceutical effect. Near-infrared （NIR） and mid-infrared （MIR） technology combined ...Rapid and sensitive recognition of herbal pieces according to different concocted processing is crucial to quality control and pharmaceutical effect. Near-infrared （NIR） and mid-infrared （MIR） technology combined with supervised pattern recognition based on partial least-squares discriminant analysis （PLSDA） was attempted to classify and recognize six different concocted processing pieces of 600 Areca catechu L. samples and the influence of fingerprint information preprocessing methods on recognition performance was also investigated in this work. Recognition rates of 99.24%, 100% and 99.49% for original fingerprint, multiple scatter correct （MSC） fingerprint and second derivative （2nd derivative） fingerprint of NIR spectra were achieved by PLSDA models, respectively. Meanwhile, a perfect recognition rate of 100% was obtained for the above three fingerprint models of MIR spectra. In conclusion, PLSDA can rapidly and effectively extract otherness of fingerprint information from NIR and MIR spectra to identify different concocted herbal pieces ofA. catechu.展开更多
Different SiGe processes and device designs are the critical influences of ionizing radiation damage. Based on the different ionizing radiation damage in SiGe HBTs fabricated by Huajie and an IBM SiGe process, quantit...Different SiGe processes and device designs are the critical influences of ionizing radiation damage. Based on the different ionizing radiation damage in SiGe HBTs fabricated by Huajie and an IBM SiGe process, quantitatively numerical simulation of ionizing radiation damage was carried out to explicate the distribution of radiation-induced charges buildup in KT9041 and IBM SiGe HBTs. The sensitive areas of the EB-spacer and isolation oxide of KT9041 are much larger than those of the IBM SiGe HBT, and the distribution of charge buildup in KT9041 is several orders of magnitude greater than that of the IBM SiGe HBT. The result suggests that the simulations are consistent with the experiment, and indicates that the geometry of the EB-spacer, the area of the Si/SiO2 interface and the isolation structure could be contributing to the different ionizing radiation damage.展开更多
Purification of surface water is widely practiced with conventional water treatment processes like coagulation-flocculation, sedimentation, filtration,and disinfection. Some reports have specified that conventional wa...Purification of surface water is widely practiced with conventional water treatment processes like coagulation-flocculation, sedimentation, filtration,and disinfection. Some reports have specified that conventional wastewater purification processes do not effectively remove many chemical contaminants,展开更多
Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SP...Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference （OIRD） in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics.展开更多
There is an apparent contrast between children’s first language acquisition and adults’second language acquisition,which are mainly manifested in the following three aspects:age difference,difference in learning pro...There is an apparent contrast between children’s first language acquisition and adults’second language acquisition,which are mainly manifested in the following three aspects:age difference,difference in learning process and motivation difference.This paper will analyze these three differences in detail,and combine the analysis results to guide second language pedagogical implications according to the current situation.展开更多
Black rice bran is a great source of dietary fiber,including soluble dietary fiber(SDF)and insoluble dietary fiber(IDF).The fermentation characteristics of dietary fiber,which significantly influence its health benefi...Black rice bran is a great source of dietary fiber,including soluble dietary fiber(SDF)and insoluble dietary fiber(IDF).The fermentation characteristics of dietary fiber,which significantly influence its health benefits,vary depending on the fiber source and processing methods.This study investigated the impact of steam explosion(SE),extrusion,and high-pressure steaming on the fermentation characteristics of IDF and SDF derived from black rice bran.The results demonstrated that all processed dietary fibers enhanced the production of short-chain fatty acids(SCFAs)after 24 h of in vitro fermentation compared to the blank control.However,when compared to untreated dietary fiber,processing resulted in a reduction in SCFA production at 24 h.Notably,SDFs consistently promoted significantly greater SCFA production than IDFs.Furthermore,both IDFs and SDFs modulated the gut microbiota composition by promoting beneficial genera such as Phascolarctobacterium,Faecalibacterium,Blautia,Bacteroides,and Butyricicoccus.IDF from extruded black rice bran notably promoted Flavonifractor,while SDF from SE treated black rice bran demonstrated superior enrichment for Faecalibacterium and Blautia.In conclusion,SE and extrusion treatments are effective methods for modifying the fermentation characteristics of black rice bran dietary fiber.This study provides a theoretical foundation for processing and utilizing black rice bran to enhance gut health.展开更多
Food-derived soybean peptides,beyond their nutritional value,are thought to have benefits in preventing alcoholic liver disease due to their antioxidant activity.However,the enzymatic preparation process suffers from ...Food-derived soybean peptides,beyond their nutritional value,are thought to have benefits in preventing alcoholic liver disease due to their antioxidant activity.However,the enzymatic preparation process suffers from low enzymatic efficiency and a lack of targeted production of protective peptides against alcoholic liver injury.This study used a pH auto-gradient drop enzymatic hydrolysis method,compared with simultaneous enzymatic hydrolysis and sequential stepwise enzymatic hydrolysis,and selected endopeptidase(alcalase,neutrase,or papain)and exopeptidase(flavourzyme)to create six different enzymatic schemes.All groups displayed a pro-tective effect on HepG2 cells exposed to alcohol-induced oxidative damage.The pH auto-gradient drop enzy-matic hydrolysis A↘P↘F group significantly decreased the ALT,AST,and LDH levels in HepG2 cells,promoted Nrf2 protein expression and reduced ROS production.Mass spectrometry analysis revealed a positive association between soybean peptide sequences enriched with His,Arg,and the presence of basic amino acids,Tyr at the C-terminus,with hepatoprotective effects in the A↘P↘F group.The pH auto-gradient drop enzymatic hydrolysis method(A↘P↘F)represents a viable alternative for preparing highly efficacious hepatoprotective active soy-bean peptides.展开更多
Digital image stabilization technique plays important roles in video surveillance and object acquisition.Many useful electronic image stabilization algorithms have been studied.A real-time algorithm is proposed based ...Digital image stabilization technique plays important roles in video surveillance and object acquisition.Many useful electronic image stabilization algorithms have been studied.A real-time algorithm is proposed based on field image gray projection which enables the regional odd and even field image to be projected into x and y directions and thus to get the regional gray projection curves in x and y directions,respectively.For the odd field image channel,motion parameters can be estimated via iterative minimum absolute difference based on two successive field image regional gray projection curves.Then motion compensations can be obtained after using the Kalman filter method.Finally,the odd field image is adjusted according to the compensations.In the mean time,motion compensation is applied to the even field image channel with the odd field image gray projection curves of the current frame.By minimizing absolute difference between odd and even field image gray projection curves of the current frame,the inter-field motion parameters can be estimated.Therefore,the even field image can be adjusted by combining the inter-field motion parameters and the odd field compensations.Finally,the stabilized image sequence can be obtained by synthesizing the adjusted odd and even field images.Experimental results show that the proposed algorithm can run in real-time and have a good stabilization performance.In addition,image blurring can be improved.展开更多
Subject Code:B02With the support of the National Natural Science Foundation of China and the Chinese Academy of Sciences,the research team led by Prof.Liu Wen(刘文)at the State Key Laboratory of Bioorganic and Natural...Subject Code:B02With the support of the National Natural Science Foundation of China and the Chinese Academy of Sciences,the research team led by Prof.Liu Wen(刘文)at the State Key Laboratory of Bioorganic and Natural Products Chemistry,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,uncovered the final biosynthetic pathway toward the distinct S-functionalization in lincosamide展开更多
Graded hypoxia is a common microenvironment in malignant solid tumors.As a central regulator in the hypoxic response,hypoxia-inducible factor-1(HIF-1)can induce multiple cellular processes including glycolysis,angioge...Graded hypoxia is a common microenvironment in malignant solid tumors.As a central regulator in the hypoxic response,hypoxia-inducible factor-1(HIF-1)can induce multiple cellular processes including glycolysis,angiogenesis,and necroptosis.How cells exploit the HIF-1 pathway to coordinate different processes to survive hypoxia remains unclear.We developed an integrated model of the HIF-1αnetwork to elucidate the mechanism of cellular adaptation to hypoxia.By numerical simulations and bifurcation analysis,we found that HIF-1αis progressively activated with worsening hypoxia due to the sequential deactivation of the hydroxylases prolyl hydroxylase domain enzymes and factor inhibiting HIF(FIH).Bistable switches control the activation and deactivation processes.As a result,glycolysis,immunosuppression,angiogenesis,and necroptosis are orderly elicited in aggravating hypoxia.To avoid the excessive accumulation of lactic acid during glycolysis,HIF-1αinduces monocarboxylate transporter and carbonic anhydrase 9 sequentially to export intracellular hydrogen ions,facilitating tumor cell survival.HIF-1α-induced miR-182 facilitates vascular endothelial growth factor production to promote angiogenesis under moderate hypoxia.The imbalance between accumulation and removal of lactic acid in severe hypoxia may result in acidosis and induce cell necroptosis.In addition,the deactivation of FIH results in the destabilization of HIF-1αin anoxia.Collectively,HIF-1αorchestrates the adaptation of tumor cells to hypoxia by selectively inducing its targets according to the severity of hypoxia.Our work may provide clues for tumor therapy by targeting the HIF-1 pathway.展开更多
Black rice bran is a good source of dietary fiber,which has many health benefits.Physicochemical properties of dietary fiber vary widely depending on their source and processing,which are crucial determinants of their...Black rice bran is a good source of dietary fiber,which has many health benefits.Physicochemical properties of dietary fiber vary widely depending on their source and processing,which are crucial determinants of their functional characteristics and potential therapeutic opportunities.In this study,black rice bran was processed by steam explosion(SE),extrusion,and high-pressure steaming to investigate the effect of different processing methods on the structural,physicochemical,and functional properties of dietary fiber from black rice bran.The results indicated that all three processing treatments could facilitate the conversion of insoluble dietary fiber(IDF)to soluble dietary fiber(SDF),significantly reduce the molecular weight of SDF,and change the monosaccharide composition of SDF,with the lowest molecular weight observed for the SDF of extruded black rice bran.Structural analyses indicated that processing led to dietary fiber samples with a looser and more porous structure.The IDF from processed black rice bran exhibited better water-holding capacity,oil-holding capacity,water swelling capacity,sodium cholate adsorption capacity,cholesterol adsorption capacity,and glucose adsorption capacity,among which IDF from black rice bran treated by extrusion and SE showed better physicochemical and functional properties.In conclusion,SE and extrusion treatment might be effective methods for dietary fiber modification in black rice bran.This study provided a theoretical basis for the deep processing and utilization of black rice bran.展开更多
基金supported by the National Natural Science Foundation of China(Nos.21205145,21276006,21036009)the Open Funds of State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University(No.201111)+1 种基金the Special Fund for Basic Scientific Research of Central Colleges,South-Central University for Nationalities(Nos.CZZ10005 and CZQ11012)the 'Five-twelfth' National Science and Technology Support Program (No.2012BAI27B00)
文摘Rapid and sensitive recognition of herbal pieces according to different concocted processing is crucial to quality control and pharmaceutical effect. Near-infrared （NIR） and mid-infrared （MIR） technology combined with supervised pattern recognition based on partial least-squares discriminant analysis （PLSDA） was attempted to classify and recognize six different concocted processing pieces of 600 Areca catechu L. samples and the influence of fingerprint information preprocessing methods on recognition performance was also investigated in this work. Recognition rates of 99.24%, 100% and 99.49% for original fingerprint, multiple scatter correct （MSC） fingerprint and second derivative （2nd derivative） fingerprint of NIR spectra were achieved by PLSDA models, respectively. Meanwhile, a perfect recognition rate of 100% was obtained for the above three fingerprint models of MIR spectra. In conclusion, PLSDA can rapidly and effectively extract otherness of fingerprint information from NIR and MIR spectra to identify different concocted herbal pieces ofA. catechu.
基金supported by the National Natural Science Foundation of China(Grant Nos.61274106 and 61574171)
文摘Different SiGe processes and device designs are the critical influences of ionizing radiation damage. Based on the different ionizing radiation damage in SiGe HBTs fabricated by Huajie and an IBM SiGe process, quantitatively numerical simulation of ionizing radiation damage was carried out to explicate the distribution of radiation-induced charges buildup in KT9041 and IBM SiGe HBTs. The sensitive areas of the EB-spacer and isolation oxide of KT9041 are much larger than those of the IBM SiGe HBT, and the distribution of charge buildup in KT9041 is several orders of magnitude greater than that of the IBM SiGe HBT. The result suggests that the simulations are consistent with the experiment, and indicates that the geometry of the EB-spacer, the area of the Si/SiO2 interface and the isolation structure could be contributing to the different ionizing radiation damage.
基金supported by grants from Science and Technology Planning Project of Shenzhen [No.200703079]
文摘Purification of surface water is widely practiced with conventional water treatment processes like coagulation-flocculation, sedimentation, filtration,and disinfection. Some reports have specified that conventional wastewater purification processes do not effectively remove many chemical contaminants,
基金Supported by the Key Research Program of Chinese Academy of Sciences
文摘Life science has a need for detection methods that are label-free and real-time. In this paper, we have selected staphylococcal protein A （SPA） and swine immunoglobulin G （IgG）, and monitor the bindings between SPA and swine IgG with different concentrations, as well as the dissociations of SPA-swine IgG complex in different pH values of phosphate buffer by oblique-incidence reflectivity difference （OIRD） in a label-free and real-time fashion. We obtain the ON and OFF reaction dynamic curves corresponding to the bindings and dissociations of SPA and swine IgG. Through our analysis of the experimental results, we have been able to obtain the damping coefficients and the dissociation time of SPA and swine IgG for different pH values of the phosphate buffer. The results prove that the OIRD technique is a competing method for monitoring the dynamic processes of biomolecule interaction and achieving the quantitative information of reaction kinetics.
文摘There is an apparent contrast between children’s first language acquisition and adults’second language acquisition,which are mainly manifested in the following three aspects:age difference,difference in learning process and motivation difference.This paper will analyze these three differences in detail,and combine the analysis results to guide second language pedagogical implications according to the current situation.
基金supported by:Guangzhou Municipal Science and Technology Plan Project(2025D04J0066)the Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science(R2019YJ-YB1003)Shenzhen Science and Technology Program(No.KCXFZ20230731100704006).
文摘Black rice bran is a great source of dietary fiber,including soluble dietary fiber(SDF)and insoluble dietary fiber(IDF).The fermentation characteristics of dietary fiber,which significantly influence its health benefits,vary depending on the fiber source and processing methods.This study investigated the impact of steam explosion(SE),extrusion,and high-pressure steaming on the fermentation characteristics of IDF and SDF derived from black rice bran.The results demonstrated that all processed dietary fibers enhanced the production of short-chain fatty acids(SCFAs)after 24 h of in vitro fermentation compared to the blank control.However,when compared to untreated dietary fiber,processing resulted in a reduction in SCFA production at 24 h.Notably,SDFs consistently promoted significantly greater SCFA production than IDFs.Furthermore,both IDFs and SDFs modulated the gut microbiota composition by promoting beneficial genera such as Phascolarctobacterium,Faecalibacterium,Blautia,Bacteroides,and Butyricicoccus.IDF from extruded black rice bran notably promoted Flavonifractor,while SDF from SE treated black rice bran demonstrated superior enrichment for Faecalibacterium and Blautia.In conclusion,SE and extrusion treatments are effective methods for modifying the fermentation characteristics of black rice bran dietary fiber.This study provides a theoretical foundation for processing and utilizing black rice bran to enhance gut health.
基金supported by the National Key Research and Devel-opment Program of China(No.2021YFD2100402).
文摘Food-derived soybean peptides,beyond their nutritional value,are thought to have benefits in preventing alcoholic liver disease due to their antioxidant activity.However,the enzymatic preparation process suffers from low enzymatic efficiency and a lack of targeted production of protective peptides against alcoholic liver injury.This study used a pH auto-gradient drop enzymatic hydrolysis method,compared with simultaneous enzymatic hydrolysis and sequential stepwise enzymatic hydrolysis,and selected endopeptidase(alcalase,neutrase,or papain)and exopeptidase(flavourzyme)to create six different enzymatic schemes.All groups displayed a pro-tective effect on HepG2 cells exposed to alcohol-induced oxidative damage.The pH auto-gradient drop enzy-matic hydrolysis A↘P↘F group significantly decreased the ALT,AST,and LDH levels in HepG2 cells,promoted Nrf2 protein expression and reduced ROS production.Mass spectrometry analysis revealed a positive association between soybean peptide sequences enriched with His,Arg,and the presence of basic amino acids,Tyr at the C-terminus,with hepatoprotective effects in the A↘P↘F group.The pH auto-gradient drop enzymatic hydrolysis method(A↘P↘F)represents a viable alternative for preparing highly efficacious hepatoprotective active soy-bean peptides.
基金supported by the National Natural Science Foundation of China(6110118561302145)
文摘Digital image stabilization technique plays important roles in video surveillance and object acquisition.Many useful electronic image stabilization algorithms have been studied.A real-time algorithm is proposed based on field image gray projection which enables the regional odd and even field image to be projected into x and y directions and thus to get the regional gray projection curves in x and y directions,respectively.For the odd field image channel,motion parameters can be estimated via iterative minimum absolute difference based on two successive field image regional gray projection curves.Then motion compensations can be obtained after using the Kalman filter method.Finally,the odd field image is adjusted according to the compensations.In the mean time,motion compensation is applied to the even field image channel with the odd field image gray projection curves of the current frame.By minimizing absolute difference between odd and even field image gray projection curves of the current frame,the inter-field motion parameters can be estimated.Therefore,the even field image can be adjusted by combining the inter-field motion parameters and the odd field compensations.Finally,the stabilized image sequence can be obtained by synthesizing the adjusted odd and even field images.Experimental results show that the proposed algorithm can run in real-time and have a good stabilization performance.In addition,image blurring can be improved.
文摘Subject Code:B02With the support of the National Natural Science Foundation of China and the Chinese Academy of Sciences,the research team led by Prof.Liu Wen(刘文)at the State Key Laboratory of Bioorganic and Natural Products Chemistry,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,uncovered the final biosynthetic pathway toward the distinct S-functionalization in lincosamide
基金supported by the National Natural Science Foundation of China(nos.12090052,11874209,11574139,and 11934008).
文摘Graded hypoxia is a common microenvironment in malignant solid tumors.As a central regulator in the hypoxic response,hypoxia-inducible factor-1(HIF-1)can induce multiple cellular processes including glycolysis,angiogenesis,and necroptosis.How cells exploit the HIF-1 pathway to coordinate different processes to survive hypoxia remains unclear.We developed an integrated model of the HIF-1αnetwork to elucidate the mechanism of cellular adaptation to hypoxia.By numerical simulations and bifurcation analysis,we found that HIF-1αis progressively activated with worsening hypoxia due to the sequential deactivation of the hydroxylases prolyl hydroxylase domain enzymes and factor inhibiting HIF(FIH).Bistable switches control the activation and deactivation processes.As a result,glycolysis,immunosuppression,angiogenesis,and necroptosis are orderly elicited in aggravating hypoxia.To avoid the excessive accumulation of lactic acid during glycolysis,HIF-1αinduces monocarboxylate transporter and carbonic anhydrase 9 sequentially to export intracellular hydrogen ions,facilitating tumor cell survival.HIF-1α-induced miR-182 facilitates vascular endothelial growth factor production to promote angiogenesis under moderate hypoxia.The imbalance between accumulation and removal of lactic acid in severe hypoxia may result in acidosis and induce cell necroptosis.In addition,the deactivation of FIH results in the destabilization of HIF-1αin anoxia.Collectively,HIF-1αorchestrates the adaptation of tumor cells to hypoxia by selectively inducing its targets according to the severity of hypoxia.Our work may provide clues for tumor therapy by targeting the HIF-1 pathway.
基金supported by the Guangdong special support program(2019BT02N112)the Special fund for scientific innovation strategyconstruction of high level Academy of Agriculture Science(R2019YJYB1003)Shenzhen Science and Technology Program(KCXFZ20230731100704006).
文摘Black rice bran is a good source of dietary fiber,which has many health benefits.Physicochemical properties of dietary fiber vary widely depending on their source and processing,which are crucial determinants of their functional characteristics and potential therapeutic opportunities.In this study,black rice bran was processed by steam explosion(SE),extrusion,and high-pressure steaming to investigate the effect of different processing methods on the structural,physicochemical,and functional properties of dietary fiber from black rice bran.The results indicated that all three processing treatments could facilitate the conversion of insoluble dietary fiber(IDF)to soluble dietary fiber(SDF),significantly reduce the molecular weight of SDF,and change the monosaccharide composition of SDF,with the lowest molecular weight observed for the SDF of extruded black rice bran.Structural analyses indicated that processing led to dietary fiber samples with a looser and more porous structure.The IDF from processed black rice bran exhibited better water-holding capacity,oil-holding capacity,water swelling capacity,sodium cholate adsorption capacity,cholesterol adsorption capacity,and glucose adsorption capacity,among which IDF from black rice bran treated by extrusion and SE showed better physicochemical and functional properties.In conclusion,SE and extrusion treatment might be effective methods for dietary fiber modification in black rice bran.This study provided a theoretical basis for the deep processing and utilization of black rice bran.
