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线粒体AAA+蛋白酶的结构与功能及其在神经系统疾病中的作用
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作者 李茹茹 张叶 +1 位作者 卫涛涛 朱笠 《生物化学与生物物理进展》 北大核心 2025年第11期2729-2748,共20页
线粒体是真核细胞中最重要的能量产生细胞器,在物质代谢、细胞信号转导、氧化应激,以及多种形式的细胞死亡途径中发挥关键的调控作用。线粒体拥有独立于细胞核基因组的DNA——线粒体DNA(mtDNA),但是只编码13条多肽链、22个转运RNA(tRNA)... 线粒体是真核细胞中最重要的能量产生细胞器,在物质代谢、细胞信号转导、氧化应激,以及多种形式的细胞死亡途径中发挥关键的调控作用。线粒体拥有独立于细胞核基因组的DNA——线粒体DNA(mtDNA),但是只编码13条多肽链、22个转运RNA(tRNA)和2个核糖体RNA(r RNA)。线粒体内的其他蛋白质都是由核基因(n DNA)编码的,这两个基因组协同工作,维持细胞的正常功能和稳态。已鉴定的人类细胞线粒体蛋白质组含有超过1158种蛋白质,它们分别定位于线粒体外膜、膜间隙、内膜和基质中,参与氧化磷酸化、三羧酸循环、分裂-融合动力学,以及维持线粒体稳态等重要功能。线粒体稳态和线粒体功能的正常发挥与线粒体蛋白酶密切相关,这些线粒体蛋白酶通过调节线粒体蛋白的活性,去除受损的或不必要的蛋白质,从而维持线粒体稳态并确保细胞存活。其中一组功能依赖于ATP结合和水解的线粒体AAA+蛋白酶(ATPases associated with diverse cellular activities,AAA+proteases),不仅执行降解错误折叠蛋白质的功能,还在线粒体融合蛋白的加工成熟、呼吸链复合物组装、mtDNA复制/转录等过程中发挥关键作用。研究发现,这些线粒体AAA+蛋白酶的基因突变或者表达异常导致其酶活性改变,严重损害线粒体结构和功能的完整性,并导致多种神经系统疾病的发生。本文主要以Lon蛋白酶1(Lon peptidase 1,LONP1)、酵母线粒体逃逸基因1样蛋白1(yeast mitochondrial escape 1 like 1,YME1L1)和ATP酶家族基因3样蛋白2(ATPase family gene 3-like 2,AFG3L2)等3种线粒体AAA+蛋白酶为例,详细阐述了它们的序列相似性和结构特点,以及在线粒体中的不同定位与功能。通过总结这3种蛋白酶基因突变与神经系统疾病的关系,发现已报道的疾病相关突变主要位于ATPase结构域和水解酶或肽酶结构域。因此,解析这些蛋白酶关键结构域的结构和突变导致的功能变化及其对线粒体乃至细胞稳态的影响,将为理解疾病机制和研发靶向干预策略提供参考。 展开更多
关键词 线粒体aaa+蛋白酶 神经系统疾病 LONP1 YME1L1 AFG3L2
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Dendritic spine degeneration:a primary mechanism in the aging process 被引量:1
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作者 Gonzalo Flores Leonardo Aguilar-Hernández +3 位作者 Fernado García-Dolores Humberto Nicolini Andrea Judith Vázquez-Hernández Hiram Tendilla-Beltrán 《Neural Regeneration Research》 SCIE CAS 2025年第6期1696-1698,共3页
Recent reports suggest that aging is not solely a physiological process in living beings;instead, it should be considered a pathological process or disease(Amorim et al., 2022). Consequently, this process involves a w... Recent reports suggest that aging is not solely a physiological process in living beings;instead, it should be considered a pathological process or disease(Amorim et al., 2022). Consequently, this process involves a wide range of factors, spanning from genetic to environmental factors, and even includes the gut microbiome(GM)(Mayer et al., 2022). All these processes coincide at some point in the inflammatory process, oxidative stress, and apoptosis, at different degrees in various organs and systems that constitute a living organism(Mayer et al., 2022;AguilarHernández et al., 2023). 展开更多
关键词 AGING process STRESS
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Prediction and optimization of flue pressure in sintering process based on SHAP 被引量:1
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作者 Mingyu Wang Jue Tang +2 位作者 Mansheng Chu Quan Shi Zhen Zhang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS 2025年第2期346-359,共14页
Sinter is the core raw material for blast furnaces.Flue pressure,which is an important state parameter,affects sinter quality.In this paper,flue pressure prediction and optimization were studied based on the shapley a... Sinter is the core raw material for blast furnaces.Flue pressure,which is an important state parameter,affects sinter quality.In this paper,flue pressure prediction and optimization were studied based on the shapley additive explanation(SHAP)to predict the flue pressure and take targeted adjustment measures.First,the sintering process data were collected and processed.A flue pressure prediction model was then constructed after comparing different feature selection methods and model algorithms using SHAP+extremely random-ized trees(ET).The prediction accuracy of the model within the error range of±0.25 kPa was 92.63%.SHAP analysis was employed to improve the interpretability of the prediction model.The effects of various sintering operation parameters on flue pressure,the relation-ship between the numerical range of key operation parameters and flue pressure,the effect of operation parameter combinations on flue pressure,and the prediction process of the flue pressure prediction model on a single sample were analyzed.A flue pressure optimization module was also constructed and analyzed when the prediction satisfied the judgment conditions.The operating parameter combination was then pushed.The flue pressure was increased by 5.87%during the verification process,achieving a good optimization effect. 展开更多
关键词 sintering process flue pressure shapley additive explanation PREDICTION OPTIMIZATION
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Remaining Life Prediction Method for Photovoltaic Modules Based on Two-Stage Wiener Process 被引量:1
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作者 Jie Lin Hongchi Shen +1 位作者 Tingting Pei Yan Wu 《Energy Engineering》 EI 2025年第1期331-347,共17页
Photovoltaic (PV) modules, as essential components of solar power generation systems, significantly influence unitpower generation costs.The service life of these modules directly affects these costs. Over time, the p... Photovoltaic (PV) modules, as essential components of solar power generation systems, significantly influence unitpower generation costs.The service life of these modules directly affects these costs. Over time, the performanceof PV modules gradually declines due to internal degradation and external environmental factors.This cumulativedegradation impacts the overall reliability of photovoltaic power generation. This study addresses the complexdegradation process of PV modules by developing a two-stage Wiener process model. This approach accountsfor the distinct phases of degradation resulting from module aging and environmental influences. A powerdegradation model based on the two-stage Wiener process is constructed to describe individual differences inmodule degradation processes. To estimate the model parameters, a combination of the Expectation-Maximization(EM) algorithm and the Bayesian method is employed. Furthermore, the Schwarz Information Criterion (SIC) isutilized to identify critical change points in PV module degradation trajectories. To validate the universality andeffectiveness of the proposed method, a comparative analysis is conducted against other established life predictiontechniques for PV modules. 展开更多
关键词 Photovoltaic modules DEGRADATION stochastic processes lifetime prediction
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An Unprecedented Efficiency with Approaching 21%Enabled by Additive‑Assisted Layer‑by‑Layer Processing in Organic Solar Cells 被引量:1
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作者 Shuai Xu Youdi Zhang +6 位作者 Yanna Sun Pei Cheng Zhaoyang Yao Ning Li Long Ye Lijian Zuo Ke Gao 《Nano-Micro Letters》 SCIE EI CAS 2025年第2期372-375,共4页
Recently published in Joule,Feng Liu and colleagues from Shanghai Jiaotong University reported a record-breaking 20.8%power conversion efficiency in organic solar cells(OSCs)with an interpenetrating fibril network act... Recently published in Joule,Feng Liu and colleagues from Shanghai Jiaotong University reported a record-breaking 20.8%power conversion efficiency in organic solar cells(OSCs)with an interpenetrating fibril network active layer morphology,featuring a bulk p-in structure and proper vertical segregation achieved through additive-assisted layer-by-layer deposition.This optimized hierarchical gradient fibrillar morphology and optical management synergistically facilitates exciton diffusion,reduces recombination losses,and enhances light capture capability.This approach not only offers a solution to achieving high-efficiency devices but also demonstrates the potential for commercial applications of OSCs. 展开更多
关键词 Organic solar cells Additive-assisted layer-by-layer processing Three-dimensional fibril morphology Bulk p-i-n structure Optical management
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The profound review of Fenton process:What’s the next step? 被引量:5
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作者 Yimin Lin Junlian Qiao +1 位作者 Yuankui Sun Hongyu Dong 《Journal of Environmental Sciences》 2025年第1期114-130,共17页
Fenton and Fenton-like processes,which could produce highly reactive species to degrade organic contaminants,have been widely used in the field of wastewater treatment.Therein,the chemistry of Fenton process including... Fenton and Fenton-like processes,which could produce highly reactive species to degrade organic contaminants,have been widely used in the field of wastewater treatment.Therein,the chemistry of Fenton process including the nature of active oxidants,the complicated reactions involved,and the behind reason for its strongly pH-dependent performance,is the basis for the application of Fenton and Fenton-like processes in wastewater treatment.Nevertheless,the conflicting views still exist about the mechanism of the Fenton process.For instance,reaching a unanimous consensus on the nature of active oxidants(hydroxyl radical or tetravalent iron)in this process remains challenging.This review comprehensively examined the mechanism of the Fenton process including the debate on the nature of active oxidants,reactions involved in the Fenton process,and the behind reason for the pH-dependent degradation of contaminants in the Fenton process.Then,we summarized several strategies that promote the Fe(Ⅱ)/Fe(Ⅲ)cycle,reduce the competitive consumption of active oxidants by side reactions,and replace the Fenton reagent,thus improving the performance of the Fenton process.Furthermore,advances for the future were proposed including the demand for the high-accuracy identification of active oxidants and taking advantages of the characteristic of target contaminants during the degradation of contaminants by the Fenton process. 展开更多
关键词 Advanced oxidation process FENTON Hydroxyl radical Fe(IV) Wastewater treatment
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基于AAA课程理论的高校英语角模型构建
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作者 张帅 《海外英语》 2025年第17期61-63,共3页
高校英语角是我国二语习得(SLA)实践中的重要环节,提升英语角建设成效是当前面临的重要议题。该研究基于文献研究以及Leo van Lier的AAA课程理论(意识性、自主性、真实性),构建了一个旨在优化高校英语角实践的模型,以期通过兴趣导向、... 高校英语角是我国二语习得(SLA)实践中的重要环节,提升英语角建设成效是当前面临的重要议题。该研究基于文献研究以及Leo van Lier的AAA课程理论(意识性、自主性、真实性),构建了一个旨在优化高校英语角实践的模型,以期通过兴趣导向、以学生为中心的英语角活动,为我国英语角的发展提出合理化建议。 展开更多
关键词 英语角 第二课堂 二语习得 高校 aaa课程理论
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The rupture process of the Hualien M 7.3 sequence on April 3,2024 被引量:1
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作者 Zhigao Yang Huifang Chen 《Earthquake Research Advances》 2025年第1期30-37,共8页
The Hualien M 7.3 earthquake on April 3,2024,was a significant and strong earthquake in Taiwan,China in the past two decades.The rupture process of the main shock and strong aftershocks is of great significance to the... The Hualien M 7.3 earthquake on April 3,2024,was a significant and strong earthquake in Taiwan,China in the past two decades.The rupture process of the main shock and strong aftershocks is of great significance to the subsequent seismic activity and seismogenic tectonic research.Based on local strong-motion data,we used the IDS(Iterative Deconvolution and Stacking)method to obtain the rupture process of the mainshock and two strong aftershocks on the 23rd.The rupture of the mainshock was mainly unilateral,lasting 31 s,with a maximum slip of 2m,and the depth of the large slip zone is about 41–49 km.There is a clear difference between the rupture depth of the main shock and the two strong aftershocks.The depths of the large slip zones of the latter two are 3–9 km and 8–10 km,respectively.There is also a significant difference in the seismogenic fault between the mainshock and the aftershocks,and we believe that there are two seismogenic fault zones in the study area,the deep and the shallow fault zone.The slip of the deep faults activates the shallow faults. 展开更多
关键词 Hualien earthquake Rupture process IDS method Focal mechanism
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Enhanced magnetic properties in a Fe-based amorphous alloy via ultrasonic vibration rapid processing 被引量:1
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作者 Hong-Zhen Li Sajad Sohrabi +4 位作者 Xin Li Lu-Yao Li Jiang Ma Huan-Lin Peng Chao Yang 《Rare Metals》 2025年第4期2853-2860,共8页
In general,the rapid growth of α-Fe clusters is a challenge in high Fe-content Fe-based amorphous alloys,negatively affecting their physical properties.Herein,we introduce an efficient and rapid post-treatment techni... In general,the rapid growth of α-Fe clusters is a challenge in high Fe-content Fe-based amorphous alloys,negatively affecting their physical properties.Herein,we introduce an efficient and rapid post-treatment technique known as ultrasonic vibration rapid processing(UVRP),which enables the formation of high-density strong magnetic α-Fe clusters,thereby enhancing the soft magnetic properties of Fe_(78)Si(13)B_(9) amorphous alloy ribbon. 展开更多
关键词 enhancing soft magnetic properties soft magnetic properties physical propertieshereinwe Fe based amorphous alloy amorphous alloy ribbon ultrasonic vibration rapid processing uvrp which Fe clusters ultrasonic vibration rapid processing
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青年扮老类语C游戏“AAA家族”的运行机制与寓教于乐价值
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作者 董浩 皇甫钰 张星宇 《山东科技大学学报(社会科学版)》 2025年第1期23-31,共9页
青年扮老类语C游戏“AAA家族”作为一种网络亚文化,不仅是青年群体自娱自乐的小圈子文化,而且是青年群体以反叛、搞怪、解构的方式对社会问题的反向言说与表达。以小红书平台上的“AAA家族”为研究对象,运用网络民族志和非结构访谈法,... 青年扮老类语C游戏“AAA家族”作为一种网络亚文化,不仅是青年群体自娱自乐的小圈子文化,而且是青年群体以反叛、搞怪、解构的方式对社会问题的反向言说与表达。以小红书平台上的“AAA家族”为研究对象,运用网络民族志和非结构访谈法,对青年扮老类语C游戏“AAA家族”的运行机制与社会价值进行探究。研究发现:青年群体之所以使用老年人语言,从表面上看,是因为在新媒体技术赋权下,通过挪用青年扮老类语C游戏“AAA家族”的语言化文字,能够以戏仿、解构等手法模仿老年人,进而在获得间接反抗父辈权威、缓和家庭关系机会的同时,还能以话语实践提供的角色扮演方式,实现代际之间的另类沟通和理解。究其本质,是因为扮老类语C游戏“AAA家族”具有寓教于乐的社会功能。即通过模仿间接获得父辈经验,进而加速个人社会化;通过自组织结成的趣缘群体,满足虚拟社交、表达、被尊重等需求;在好奇心的驱使下与解构的游戏狂欢中,通过模仿提前获得了“老年化”的体验,进而实现理解父辈、促进代际沟通的作用;通过碰撞与交融的方式,促进了网络青年亚文化与主流文化的融合。 展开更多
关键词 aaa家族 青年 戏仿 语C游戏 亚文化
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Buoyancy characteristic analysis and optimization of precast concrete slab track during casting process of self-compacting concrete 被引量:1
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作者 Pengsong Wang Tao Xin +2 位作者 Peng Chen Sen Wang Di Cheng 《Railway Sciences》 2025年第2期159-173,共15页
Purpose–The precast concrete slab track(PST)has advantages of fewer maintenance frequencies,better smooth rides and structural stability,which has been widely applied in urban rail transit.Precise positioning of prec... Purpose–The precast concrete slab track(PST)has advantages of fewer maintenance frequencies,better smooth rides and structural stability,which has been widely applied in urban rail transit.Precise positioning of precast concrete slab(PCS)is vital for keeping the initial track regularity.However,the cast-in-place process of the self-compacting concrete(SCC)filling layer generally causes a large deformation of PCS due to the water-hammer effect of flowing SCC,even cracking of PCS.Currently,the buoyancy characteristic and influencing factors of PCS during the SCC casting process have not been thoroughly studied in urban rail transit.Design/methodology/approach–In this work,a Computational Fluid Dynamics(CFD)model is established to calculate the buoyancy of PCS caused by the flowing SCC.The main influencing factors,including the inlet speed and flowability of SCC,have been analyzed and discussed.A new structural optimization scheme has been proposed for PST to reduce the buoyancy caused by the flowing SCC.Findings–The simulation and field test results showed that the buoyancy and deformation of PCS decreased obviously after adopting the new scheme.Originality/value–The findings of this study can provide guidance for the control of the deformation of PCS during the SCC construction process. 展开更多
关键词 Casting process Buoyancy characteristics Precast concrete slab track SIMULATION Field test OPTIMIZATION
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Adsorption-Driven Interfacial Interactions: The Key to Enhanced Performance in Heterogeneous Advanced Oxidation Processes 被引量:1
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作者 Jinming Luo Deyou Yu +3 位作者 Kaixing Fu Zhuoya Fang Xiaolin Zhang Mingyang Xing 《Engineering》 2025年第4期22-25,共4页
Current research on heterogeneous advanced oxidation processes(HAOPs)predominantly emphasizes catalyst iteration and innovation.Significant efforts have been made to regulate the electron structure and optimize the el... Current research on heterogeneous advanced oxidation processes(HAOPs)predominantly emphasizes catalyst iteration and innovation.Significant efforts have been made to regulate the electron structure and optimize the electron distribution,thereby increasing the catalytic activity.However,this focus often overshadows an equally essential aspect of HAOPs:the adsorption effect.Adsorption is a critical initiator for triggering the interaction of oxidants and contaminants with heterogeneous catalysts.The efficacy of these interactions is influenced by a variety of physicochemical properties,including surface chemistry and pore sizes,which determine the affinities between contaminants and material surfaces.This dispar ity in affinity is pivotal because it underpins the selective removal of contaminants,especially in complex waste streams containing diverse contaminants and competing matrices.Consequently,understanding and mastering these interfacial interactions is fundamentally indispensable not only for improving pro cess efficiency but also for enhancing the selectivity of contaminant removal.Herein,we highlight the importance of adsorption-driven interfacial interactions for fundamentally elucidating the catalytic mechanisms of HAOPs.Such interactions dictate the overall performance of the treatment processes by balancing the adsorption,reaction,and desorption rates on the catalyst surfaces.Elucidating the adsorption effect not only shifts the paradigm in understanding HAOPs but also improves their practical ity in water treatment and wastewater decontamination.Overall,we propose that revisiting adsorption driven interfacial interactions holds great promise for optimizing catalytic processes to develop effective HAOP strategies. 展开更多
关键词 Heterogeneous advanced oxidation processES ADSORPTION Pollutant degradation Dual active sites CATALYSIS SELECTIVITY
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Relative vacuum reduction innovative processes applied in primary magnesium production-Comprehensive analysis of thermodynamics,resource,energy flow,and carbon emission 被引量:1
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作者 Xiaolong Li Tingan Zhang +3 位作者 Yan Liu Junhua Guo Jingzhong Xu Yuanyuan Liang 《Journal of Magnesium and Alloys》 2025年第7期3134-3149,共16页
Magnesium and magnesium alloys,serving as crucial lightweight structural materials and hydrogen storage elements,find extensive applications in space technology,aviation,automotive,and magnesium-based hydrogen industr... Magnesium and magnesium alloys,serving as crucial lightweight structural materials and hydrogen storage elements,find extensive applications in space technology,aviation,automotive,and magnesium-based hydrogen industries.The global production of primary magnesium has reached approximately 1.2 million tons per year,with anticipated diversification in future applications and significant market demand.Nevertheless,approximately 80%of the world’s primary magnesium is still manufactured through the Pidgeon process,grappling with formidable issues including high energy consumption,massive carbon emission,significant resource depletion,and environmental pollution.The implementation of the relative vacuum method shows potential in breaking through technological challenges in the Pidgeon process,facilitating clean,low-carbon continuous magnesium smelting.This paper begins by introducing the principles of the relative vacuum method.Subsequently,it elucidates various innovative process routes,including relative vacuum ferrosilicon reduction,aluminum thermal reduction co-production of spinel,and aluminum thermal reduction co-production of calcium aluminate.Finally,and thermodynamic foundations of the relative vacuum,a quantitative analysis of the material,energy flows,carbon emission,and production cost for several new processes is conducted,comparing and analyzing them against the Pidgeon process.The study findings reveal that,with identical raw materials,the relative vacuum silicon thermal reduction process significantly decreases raw material consumption,energy consumption,and carbon dioxide emissions by 15.86%,30.89%,and 26.27%,respectively,compared to the Pidgeon process.The relative vacuum process,using magnesite as the raw material and aluminum as the reducing agent,has the lowest magnesium-to-feed ratio,at only 3.385.Additionally,its energy consumption and carbon dioxide emissions are the lowest,at 1.817 tce/t Mg and 7.782 t CO_(2)/t Mg,respectively.The energy consumption and carbon emissions of the relative vacuum magnesium smelting process co-producing calcium aluminate(12CaO·7Al_(2)O_(3),3CaO·Al_(2)O_(3),and CaO·Al_(2)O_(3))are highly correlated with the consumption of dolomite in the raw materials.When the reduction temperature is around 1473.15 K,the critical volume fraction of magnesium vapor for different processes varies within the range of 5%–40%.Production cost analysis shows that the relative vacuum primary magnesium smelting process has significant economic benefits.This paper offers essential data support and theoretical guidance for achieving energy efficiency,carbon reduction in magnesium smelting,and the industrial adoption of innovative processes. 展开更多
关键词 Magnesium smelting Relative vacuum reduction process THERMODYNAMICS Resource and energy flow Carbon emission
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A new multi-DOF envelope forming process for fabricating spiral bevel gears with back taper tooth 被引量:1
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作者 Xinghui HAN Yanhui WANG +3 位作者 Lin HUA Wuhao ZHUANG Fangyan ZHENG Wei FENG 《Chinese Journal of Aeronautics》 2025年第1期568-585,共18页
Spiral bevel gears are critical transmission components,and are widely used in the aerospace field.This paper proposes a new multi-DOF envelope forming process for fabricating spiral bevel gears.Firstly,the multi-DOF ... Spiral bevel gears are critical transmission components,and are widely used in the aerospace field.This paper proposes a new multi-DOF envelope forming process for fabricating spiral bevel gears.Firstly,the multi-DOF envelope forming principle of spiral bevel gears is proposed.Secondly,the design methods for the envelope tool geometry and movement are proposed based on the envelope geometry and movement relationships.Thirdly,the metal flow and tooth filling laws are revealed through 3D FE simulation of the multi-DOF envelope forming process of a typical spiral bevel gear.Fourthly,a new method for separating the envelope tool and the formed spiral bevel gear with back taper tooth is proposed to avoid their interference.Finally,experiments on multi-DOF envelope forming of this typical spiral bevel gear are conducted using new heavy load multi-DOF envelope forming equipment.The simulation and experimental results show the feasibility of the proposed multi-DOF envelope forming process for fabricating spiral bevel gears with back taper tooth and the corresponding process design methods. 展开更多
关键词 Multi-DOF envelope forming Spiral bevel gear Back taper tooth Metal flow process design
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RuvB样AAA ATP酶2通过激活AKT/mTOR信号通路促进肺腺癌细胞增殖、迁移及侵袭
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作者 黄楚红 程贞 +5 位作者 卢惠浩 董佳丽 林燕明 陈玉婷 李姝君 杨志雄 《肿瘤学杂志》 2025年第5期390-400,共11页
[目的]探讨RuvB样AAA ATP酶2(RuvB-like AAA ATPase 2,RUVBL2)在肺腺癌中的表达及与预后的相关性,并进一步研究其对肺腺癌细胞系A549及H1299细胞增殖、迁移、侵袭能力的影响,及其发挥生物学功能的内在机制。[方法]通过R语言信息技术对... [目的]探讨RuvB样AAA ATP酶2(RuvB-like AAA ATPase 2,RUVBL2)在肺腺癌中的表达及与预后的相关性,并进一步研究其对肺腺癌细胞系A549及H1299细胞增殖、迁移、侵袭能力的影响,及其发挥生物学功能的内在机制。[方法]通过R语言信息技术对癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库、UALCAN数据库(http://ualcan.path.uab.edu)等分析RUVBL2在肺腺癌及肺组织中的表达及与临床病理学参数间的关系。构建Cox风险回归模型,探讨影响肺腺癌生存的预后因素。利用基因表达谱交互分析2(gene expression profiling interactive analysis 2,GEPIA2)绘制RUVBL2表达与预后的生存曲线。通过在线网站癌症细胞系百科全书(Cancer Cell Line Encyclopedia,CCLE)查询肺癌RUVBL2基因表达量,选择A549及H1299细胞模型进行后续研究。转染siRNA,分为阴性对照组、实验组(siRNA-RUVBL2,si-RUVBL2)。采用细胞计数试剂盒-8(cell counting kit-8,CCK8)法检测细胞增殖能力,采用Transwell、划痕实验检测细胞迁移侵袭能力,并通过R软件对TCGA高通量测序数据中RUVBL2表达相关差异基因进行基于基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)的基因集富集(gene set enrichment analysis,GSEA)分析寻找RUVBL2可能参与调控的通路,通过蛋白免疫印迹实验(Western blot,WB)验证相关通路。通过10例配对的肺腺癌患者癌组织与癌旁组织样本的WB实验验证RUVBL2表达差异。[结果]RUVBL2在肺腺癌中表达显著性高于癌旁组织,与肺腺癌患者不良的临床预后相关;RUVBL2可作为肺腺癌预后的独立风险因素。在肺腺癌细胞系A549细胞中沉默RUVBL2后,增殖能力降低,但差异无统计学意义(P=0.264);而在H1299细胞中沉默RUVBL2后,增殖能力显著性下降(P=0.001)。肺腺癌细胞系A549及H1299细胞沉默RUVBL2后,迁移、侵袭能力显著性减弱(P<0.001)。在沉默RUVBL2后,磷酸化的蛋白激酶B(phosphor-protein kinase B,p-AKT)及磷酸化的哺乳动物雷帕霉素靶蛋白(phosphor-mammalian target of rapamycin,p-mTOR)蛋白表达受到显著性抑制。临床组织样本结果验证了RUVBL2在肺腺癌组织高表达。[结论]RUVBL2在肺腺癌高表达,可作为预测肺腺癌预后的独立危险因素,预示不良的临床预后。RUVBL2可能通过激活AKT/mTOR信号转导通路影响肺腺癌细胞系A549及H1299的增殖、迁移及侵袭。 展开更多
关键词 肺腺癌 RuvB样aaa ATP酶2 AKT/MTOR 增殖 迁移侵袭
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Towards a blank design method for manufacturing big-tapered profiled ring disk by spinning-rolling process 被引量:1
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作者 Xuechao LI Lianggang GUO +1 位作者 Xiaoqing CHEN Heng LI 《Chinese Journal of Aeronautics》 2025年第1期70-86,共17页
The big-tapered profiled ring disk is a key component of engines for rockets and missiles.A new forming technology,as called spinning-rolling process,has been proposed previously for the high performance,high efficien... The big-tapered profiled ring disk is a key component of engines for rockets and missiles.A new forming technology,as called spinning-rolling process,has been proposed previously for the high performance,high efficiency and low-cost manufacturing of the component.Blank design is the key part of plastic forming process design.For spinning-rolling process,the shape and size of the blank play a crucial role in process stability,deformation behavior and dimensional accuracy.So this work proposes a blank design method to determine the geometry structure and sizes of the blank.The mathematical model for calculating the blank size has been deduced based on volume conservation and neutral layer length invariance principle.The FE simulation and corresponding trial production of an actual big-tapered profiled ring disk show that the proposed blank design method is applicative.In order to obtain a preferred blank,the influence rules of blank size determined by different deformation degrees(rolling ratio k)on the spinning-rolling process are revealed by comprehensive FE simulations.Overall considering the process stability,circularity of the deformed ring disk and forming forces,a reasonable range of deformation degree(rolling ratio k)is recommended for the blank design of the new spinning-rolling process. 展开更多
关键词 Blank design method Spinning-rolling process Big-tapered profiled ring disk Rolling ratio Intelligent FE simulation
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Optimization of Surface Layer Properties of Mg-9Li-1Zn Alloy by Ultrasonic Surface Rolling Process and its Impact on Corrosion Behavior 被引量:1
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作者 Huimin Yang Kun Yang +1 位作者 Guobing Wei Rongguang Li 《Acta Metallurgica Sinica(English Letters)》 2025年第8期1421-1435,共15页
The Mg-9Li-1Zn(LZ91)alloy was subjected to an ultrasonic surface rolling process(USRP)with varying passes for the purpose of modifying its surface state.The USRP transformed surface residual stress from initial tensil... The Mg-9Li-1Zn(LZ91)alloy was subjected to an ultrasonic surface rolling process(USRP)with varying passes for the purpose of modifying its surface state.The USRP transformed surface residual stress from initial tensile stress to compressive stress,decreasing the surface roughness and increasing the ratio of the β-Li phase.The USRPed LZ91 sample(3 passes)showed superior corrosion resistance,with the corrosion current density changing from 57.11 to 24.70μA cm^(-2),and the polarization resistance increasing from 576.3 to 1146.1Ωcm^(2).According to the corrosion procedure evaluations,in situ observation revealed that the LZ91 alloy initially experiences pitting,which subsequently develops into cracking.The substantial area coverage of the β-Li phase facilitates the formation of a protective film on the surface,effectively delaying localized corrosion. 展开更多
关键词 Dual-phase Mg-Li alloy Ultrasonic surface rolling process Oxide film Local corrosion Compressive residual stress
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Multi-objective optimization of grinding process parameters for improving gear machining precision 被引量:1
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作者 YOU Tong-fei HAN Jiang +4 位作者 TIAN Xiao-qing TANG Jian-ping LU Yi-guo LI Guang-hui XIA Lian 《Journal of Central South University》 2025年第2期538-551,共14页
The gears of new energy vehicles are required to withstand higher rotational speeds and greater loads,which puts forward higher precision essentials for gear manufacturing.However,machining process parameters can caus... The gears of new energy vehicles are required to withstand higher rotational speeds and greater loads,which puts forward higher precision essentials for gear manufacturing.However,machining process parameters can cause changes in cutting force/heat,resulting in affecting gear machining precision.Therefore,this paper studies the effect of different process parameters on gear machining precision.A multi-objective optimization model is established for the relationship between process parameters and tooth surface deviations,tooth profile deviations,and tooth lead deviations through the cutting speed,feed rate,and cutting depth of the worm wheel gear grinding machine.The response surface method(RSM)is used for experimental design,and the corresponding experimental results and optimal process parameters are obtained.Subsequently,gray relational analysis-principal component analysis(GRA-PCA),particle swarm optimization(PSO),and genetic algorithm-particle swarm optimization(GA-PSO)methods are used to analyze the experimental results and obtain different optimal process parameters.The results show that optimal process parameters obtained by the GRA-PCA,PSO,and GA-PSO methods improve the gear machining precision.Moreover,the gear machining precision obtained by GA-PSO is superior to other methods. 展开更多
关键词 worm wheel gear grinding machine gear machining precision machining process parameters multi objective optimization
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Superplastic behavior of fine-grained Ti-10V-2Fe-3Al alloy fabricated by friction stir processing 被引量:1
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作者 Kai Wang Wenjing Zhang +3 位作者 Takuya Ogura Yoshiaki Morisada Xinqing Zhao Hidetoshi Fujii 《Journal of Materials Science & Technology》 2025年第3期26-36,共11页
Ti-10V-2Fe-3Al alloy with fine-grainedβphases was fabricated by friction stir processing with opti-mized processing parameters.The superplastic behavior of the specimens was investigated by tensile deformation at dif... Ti-10V-2Fe-3Al alloy with fine-grainedβphases was fabricated by friction stir processing with opti-mized processing parameters.The superplastic behavior of the specimens was investigated by tensile deformation at different strain rates and temperatures,and an optimal superplastic elongation of 634%was achieved at 700℃ and 3×10^(-4)/s.An annealing treatment at 650℃ for 60 min showed a mi-crostructure withαprecipitates distributed in theβmatrix in the friction stir specimen.Such pre-heat treatment improves the superplasticity of the specimen,achieving an elongation of up to 807%at 750℃ and 3×10^(-4)/s.The influences of tensile temperatures and strain rates on the microstructural evolution,such as grain size variation,grain morphology,and phase transformations,were discussed.The super-plastic deformation behavior of fine-grained Ti-10V-2Fe-3Al alloy is controlled by grain boundary sliding and accompanied by dynamic phase transformation and recrystallization. 展开更多
关键词 Ti-10V-2Fe-3Al alloy Friction stir processing SUPERPLASTICITY Microstructural evolution Pre-heat treatment
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Hot Deformation Behavior and Processing Map of Zr-Sn-Nb-Fe-Cr Alloy
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作者 Li Yuli Li Hao +3 位作者 Chen Qiuyu Zhou Xuan Ma Tianyang Sun Taotao 《稀有金属材料与工程》 北大核心 2025年第7期1671-1677,共7页
The hot deformation characteristics of induction quenched Zr-Sn-Nb-Fe-Cr alloy forged rod in the temperature range of 600–900°C and strain rate range of 0.001–1 s^(-1)were studied by Gleeble3800 uniaxial hot co... The hot deformation characteristics of induction quenched Zr-Sn-Nb-Fe-Cr alloy forged rod in the temperature range of 600–900°C and strain rate range of 0.001–1 s^(-1)were studied by Gleeble3800 uniaxial hot compression experiment.The results show that the flow stress decreases with the decrease in strain rate and the increase in deformation temperature in the true stress-true strain curve of Zr-Sn-Nb-Fe-Cr alloy forged rod.Moreover,the hot deformation characteristics of the material can be described by the hyperbolic sine constitutive equation.Under the experimental conditions,the average thermal activation energy(Q)of the alloy was 412.9105 kJ/mol.The microstructure analysis of the processing map and the sample after hot compression shows that the optimum hot working parameters of the alloy are 795–900°C,0.001–0.0068 s^(-1),at the deformation temperature of 600–900°C,and the strain rate of 0.001–1 s^(-1). 展开更多
关键词 zirconium alloy constitutive equation hot deformation processing map
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