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Graph Guide Diffusion Solvers with Noises for Travelling Salesman Problem
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作者 Yan Kong Xinpeng Guo Chih-Hsien Hsia 《Computers, Materials & Continua》 2026年第3期689-707,共19页
With the development of technology,diffusion model-based solvers have shown significant promise in solving Combinatorial Optimization(CO)problems,particularly in tackling Non-deterministic Polynomial-time hard(NP-hard... With the development of technology,diffusion model-based solvers have shown significant promise in solving Combinatorial Optimization(CO)problems,particularly in tackling Non-deterministic Polynomial-time hard(NP-hard)problems such as the Traveling Salesman Problem(TSP).However,existing diffusion model-based solvers typically employ a fixed,uniform noise schedule(e.g.,linear or cosine annealing)across all training instances,failing to fully account for the unique characteristics of each problem instance.To address this challenge,we present GraphGuided Diffusion Solvers(GGDS),an enhanced method for improving graph-based diffusion models.GGDS leverages Graph Neural Networks(GNNs)to capture graph structural information embedded in node coordinates and adjacency matrices,dynamically adjusting the noise levels in the diffusion model.This study investigates the TSP by examining two distinct time-step noise generation strategies:cosine annealing and a Neural Network(NN)-based approach.We evaluate their performance across different problem scales,particularly after integrating graph structural information.Experimental results indicate that GGDS outperforms previous methods with average performance improvements of 18.7%,6.3%,and 88.7%on TSP-500,TSP-100,and TSP-50,respectively.Specifically,GGDS demonstrates superior performance on TSP-500 and TSP-50,while its performance on TSP-100 is either comparable to or slightly better than that of previous methods,depending on the chosen noise schedule and decoding strategy. 展开更多
关键词 Combinatorial optimization problem diffusion model noise schedule traveling salesman problem
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Mindfulness and internalizing/externalizing problems among adolescents:Ethnicity moderation and psychological capital mediation
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作者 Jia Wu Wei Luo +4 位作者 Qianguo Xiao Qinhong Xie Xiaodong Li Taiyong Bi Hui Kou 《Journal of Psychology in Africa》 2026年第1期97-106,共10页
This study examines the mediating role of positive psychological capital and the moderating role of ethnicity in the relationship between mindfulness and internalizing/externalizing problems among adolescents.The stud... This study examines the mediating role of positive psychological capital and the moderating role of ethnicity in the relationship between mindfulness and internalizing/externalizing problems among adolescents.The study sample comprized Chinese adolescents(N=637 ethnic minority;females=40.97%,meam age=12.68,SD=0.49 years;N=636 Han;females=49.06%,mean age=12.71,SD=0.47 years).The participants completed the Child and Adolescent Mindfulness Measure,the Positive Psycap Questionnaire,and the Youth Self-Report.Results from the moderated mediation analysis showed mindfulness was negatively associated with both internalizing and externalizing problems.Ethnicity moderated the relationship between mindfulness and internalizing problems to be stronger for Han adolescents compared to ethnic minority adolescents.Psychological capital mediated the relationship between mindfulness and internalizing problems in both groups,with a negative direction.Findings support the Conservation of Resources theory and highlight mindfulness as a personal resource fostering adolescent well-being in multicultural contexts. 展开更多
关键词 Ethnic minority adolescent MINDFULNESS positive psychological capital internalizing problems externalizing problems
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Solutions of three-body problem based on an equivalent system approach
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作者 Jing Tang Xing 《Acta Mechanica Sinica》 2026年第1期292-309,共18页
Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fund... Generalised reduced masses with a set of equations governing the three relative motions between two of 3-bodies in their gravitational field are established,of which the dynamic characteristics of 3-body dynamics,fundamental bases of this paper,are revealed.Based on these findings,an equivalent system is developed,which is a 2-body system with its total mass,constant angular momentum,kinetic and potential energies same as the total ones of three relative motions,so that it can be solved using the well-known theory of the 2-body system.From the solution of an equivalent system with the revealed characteristics of three relative motions,the general theoretical solutions of the 3-body system are obtained in the curve-integration forms along the orbits in the imaged radial motion space.The possible periodical orbits with generalised Kepler’s law are presented.Following the description and mathematical demonstrations of the proposed methods,the examples including Euler’s/Lagrange’s problems,and a reported numerical one are solved to validate the proposed methods.The methods derived from the 3-body system are extended to N-body problems. 展开更多
关键词 Three-body problem Equivalent system with solutions Orbit-equation of a conic section Generalised reduced mass Chaotic motions Generalised Kepler’s law N-body problem
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Collaborative scheduling problem pertaining to launch and recovery operations for carrier aircraft
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作者 GUO Fang HAN Wei +3 位作者 LIU Yujie SU Xichao LIU Jie LI Changjiu 《Journal of Systems Engineering and Electronics》 2026年第1期287-306,共20页
The proliferation of carrier aircraft and the integration of unmanned aerial vehicles(UAVs)on aircraft carriers present new challenges to the automation of launch and recovery operations.This paper investigates a coll... The proliferation of carrier aircraft and the integration of unmanned aerial vehicles(UAVs)on aircraft carriers present new challenges to the automation of launch and recovery operations.This paper investigates a collaborative scheduling problem inherent to the operational processes of carrier aircraft,where launch and recovery tasks are conducted concurrently on the flight deck.The objective is to minimize the cumulative weighted waiting time in the air for recovering aircraft and the cumulative weighted delay time for launching aircraft.To tackle this challenge,a multiple population self-adaptive differential evolution(MPSADE)algorithm is proposed.This method features a self-adaptive parameter updating mechanism that is contingent upon population diversity,an asynchronous updating scheme,an individual migration operator,and a global crossover mechanism.Additionally,comprehensive experiments are conducted to validate the effectiveness of the proposed model and algorithm.Ultimately,a comparative analysis with existing operation modes confirms the enhanced efficiency of the collaborative operation mode. 展开更多
关键词 carrier aircraft collaborative scheduling problem LAUNCH RECOVERY multiple population differential evolution
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Centralized Circumcentered-Reflection Method for Solving the Convex Feasibility Problem in Sparse Signal Recovery
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作者 Chunmei LI Bangjun CHEN Xuefeng DUAN 《Journal of Mathematical Research with Applications》 2026年第1期119-133,共15页
Convex feasibility problems are widely used in image reconstruction, sparse signal recovery, and other areas. This paper is devoted to considering a class of convex feasibility problem arising from sparse signal recov... Convex feasibility problems are widely used in image reconstruction, sparse signal recovery, and other areas. This paper is devoted to considering a class of convex feasibility problem arising from sparse signal recovery. We first derive the projection formulas for a vector onto the feasible sets. The centralized circumcentered-reflection method is designed to solve the convex feasibility problem. Some numerical experiments demonstrate the feasibility and effectiveness of the proposed algorithm, showing superior performance compared to conventional alternating projection methods. 展开更多
关键词 convex feasibility problem centralized circumcentered-re ection method sparse signal recovery compressed sensing
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Quality-guaranteed Dubins Path Planning for USV Based on Mixed-integer Piecewise linear Programming for Addressing the Extended Minimum-time Intercept Problem
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作者 Xing Zhou Kelin Zhu +3 位作者 Shuang Liu Zhaoqing Li Wenxin Zhang Kang Du 《哈尔滨工程大学学报(英文版)》 2026年第1期216-227,共12页
During the use of robotics in applications such as antiterrorism or combat,a motion-constrained pursuer vehicle,such as a Dubins unmanned surface vehicle(USV),must get close enough(within a prescribed zero or positive... During the use of robotics in applications such as antiterrorism or combat,a motion-constrained pursuer vehicle,such as a Dubins unmanned surface vehicle(USV),must get close enough(within a prescribed zero or positive distance)to a moving target as quickly as possible,resulting in the extended minimum-time intercept problem(EMTIP).Existing research has primarily focused on the zero-distance intercept problem,MTIP,establishing the necessary or sufficient conditions for MTIP optimality,and utilizing analytic algorithms,such as root-finding algorithms,to calculate the optimal solutions.However,these approaches depend heavily on the properties of the analytic algorithm,making them inapplicable when problem settings change,such as in the case of a positive effective range or complicated target motions outside uniform rectilinear motion.In this study,an approach employing a high-accuracy and quality-guaranteed mixed-integer piecewise-linear program(QG-PWL)is proposed for the EMTIP.This program can accommodate different effective interception ranges and complicated target motions(variable velocity or complicated trajectories).The high accuracy and quality guarantees of QG-PWL originate from elegant strategies such as piecewise linearization and other developed operation strategies.The approximate error in the intercept path length is proved to be bounded to h^(2)/(4√2),where h is the piecewise length. 展开更多
关键词 Minimum-time intercept problem Dubins vehicle Mixed-integer piecewise-linear program LINEARIZATION Approximate error trigonometric function USV
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Cooperative Metaheuristics with Dynamic Dimension Reduction for High-Dimensional Optimization Problems
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作者 Junxiang Li Zhipeng Dong +2 位作者 Ben Han Jianqiao Chen Xinxin Zhang 《Computers, Materials & Continua》 2026年第1期1484-1502,共19页
Owing to their global search capabilities and gradient-free operation,metaheuristic algorithms are widely applied to a wide range of optimization problems.However,their computational demands become prohibitive when ta... Owing to their global search capabilities and gradient-free operation,metaheuristic algorithms are widely applied to a wide range of optimization problems.However,their computational demands become prohibitive when tackling high-dimensional optimization challenges.To effectively address these challenges,this study introduces cooperative metaheuristics integrating dynamic dimension reduction(DR).Building upon particle swarm optimization(PSO)and differential evolution(DE),the proposed cooperative methods C-PSO and C-DE are developed.In the proposed methods,the modified principal components analysis(PCA)is utilized to reduce the dimension of design variables,thereby decreasing computational costs.The dynamic DR strategy implements periodic execution of modified PCA after a fixed number of iterations,resulting in the important dimensions being dynamically identified.Compared with the static one,the dynamic DR strategy can achieve precise identification of important dimensions,thereby enabling accelerated convergence toward optimal solutions.Furthermore,the influence of cumulative contribution rate thresholds on optimization problems with different dimensions is investigated.Metaheuristic algorithms(PSO,DE)and cooperative metaheuristics(C-PSO,C-DE)are examined by 15 benchmark functions and two engineering design problems(speed reducer and composite pressure vessel).Comparative results demonstrate that the cooperative methods achieve significantly superior performance compared to standard methods in both solution accuracy and computational efficiency.Compared to standard metaheuristic algorithms,cooperative metaheuristics achieve a reduction in computational cost of at least 40%.The cooperative metaheuristics can be effectively used to tackle both high-dimensional unconstrained and constrained optimization problems. 展开更多
关键词 Dimension reduction modified principal components analysis high-dimensional optimization problems cooperative metaheuristics metaheuristic algorithms
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Non-Newtonian rivulet flows on an inclined planar surface applying the 2nd Stokes problem
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作者 S.V.ERSHKOV E.S.BARANOVSKII A.V.YUDIN 《Applied Mathematics and Mechanics(English Edition)》 2026年第1期153-164,共12页
The newly formulated non-Newtonian rivulet flows streaming down an inclined planar surface,with additional periodic perturbations arising from the application of the 2nd Stokes problem to the investigation of rivulet ... The newly formulated non-Newtonian rivulet flows streaming down an inclined planar surface,with additional periodic perturbations arising from the application of the 2nd Stokes problem to the investigation of rivulet dynamics,are demonstrated in the current research.Hereby,the 2nd Stokes problem assumes that the surface,with a thin shared layer of the fluid on it,oscillates in a harmonic manner along the x-axis of the rivulet flow,which coincides with the main flow direction streaming down the underlying surface.We obtain the exact extension of the rivulet flow family,clarifying the structure of the pressure field,which fully absorbs the arising perturbation.The profile of the velocity field is assumed to be Gaussian-type with a non-zero level of plasticity.Hence,the absolutely non-Newtonian case of the viscoplastic flow solution,which satisfies the motion and continuity equations,is considered(with particular cases of exact solutions for pressure).The perturbed governing equations of motion for rivulet flows then result in the Riccati-type ordinary differential equation(ODE),describing the dynamics of the coordinate x(t).The approximated schematic dynamics are presented in graphical plots. 展开更多
关键词 rivulet flow non-Newtonian fluid creeping viscoplastic flow 1st/2nd Stokes problem
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AUGMENTED SUBSPACE SCHEME FOR EIGENVALUE PROBLEM BY WEAK GALERKIN FINITE ELEMENT METHOD
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作者 Yue Feng Zhijin Guan +1 位作者 Hehu Xie Chenguang Zhou, 《Journal of Computational Mathematics》 2026年第1期135-164,共30页
This study proposes a class of augmented subspace schemes for the weak Galerkin(WG)finite element method used to solve eigenvalue problems.The augmented subspace is built with the conforming linear finite element spac... This study proposes a class of augmented subspace schemes for the weak Galerkin(WG)finite element method used to solve eigenvalue problems.The augmented subspace is built with the conforming linear finite element space defined on the coarse mesh and the eigen-function approximations in the WG finite element space defined on the fine mesh.Based on this augmented subspace,solving the eigenvalue problem in the fine WG finite element space can be reduced to the solution of the linear boundary value problem in the same WG finite element space and a low dimensional eigenvalue problem in the augmented sub-space.The proposed augmented subspace techniques have the second order convergence rate with respect to the coarse mesh size,as demonstrated by the accompanying error esti-mates.Finally,a few numerical examples are provided to validate the proposed numerical techniques. 展开更多
关键词 Eigenvalue problem Augmented subspace scheme Weak Galerkin finite ele-ment method Second order convergence rate
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Major Problems in American Education: From a Socio-Cultural Perspective
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作者 丁晓蔚 《海外英语》 2013年第23期322-323,328,共3页
American intellectuals of different generations and in various eras,with a strong sense of social responsibility and a critical mind,have dedicated themselves in revealing some major problems existing in American educ... American intellectuals of different generations and in various eras,with a strong sense of social responsibility and a critical mind,have dedicated themselves in revealing some major problems existing in American education,namely educational inequality,declining academic performance,inadequate funding,and school violence.By describing and probing into these problems from a socio-cultural perspective,they offer a solution of eliminating the socio-economic inequality in America,which is fundamental and essential. 展开更多
关键词 AMERICAN EDUCATION problemS SOCIO-CULTURAL perspec
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Problem regions and regional problems of socioeconomic development in China: A perspective from the coordinated development of industrialization, informatization, urbanization and agricultural modernization 被引量:26
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作者 LI Yurui WANG Jing +1 位作者 LIU Yansui LONG Hualou 《Journal of Geographical Sciences》 SCIE CSCD 2014年第6期1115-1130,共16页
Identifying the problem regions and regional problems, and thus improving regional policies, are crucial for the sustainable development of various economic entities. The coordinated development of industrialization, ... Identifying the problem regions and regional problems, and thus improving regional policies, are crucial for the sustainable development of various economic entities. The coordinated development of industrialization, informatization, urbanization and agricultural modernization(hereinafter referred to as "Sihua") is not only a practical need but an important strategic direction of integrating urban-rural development and regional development in recent China, and it also provides a significant perspective for identifying problem regions and regional problems so as to improve the regional policies. This study mainly aims to: firstly, establish a comprehensive evaluation index system so as to explore the spatial pattern of coordinated development of Sihua in China at prefecture level; secondly, to develop an evaluation criteria system to identify the problem regions and regional problems from the perspective of coordinated development of Sihua. This paper comes first in the scientific community to evaluate the coordinated development state of Sihua in China at prefecture level and identify the problem regions and regional problems from the perspective of Sihua development by quantitative analysis. This study may benefit the improvement of regional policies and thus contribute to the sustainable socio-economic development of China. 展开更多
关键词 problem regions regional problems regional policies industrialization informatization urbanization agricultural modernization
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Exosomes in neurological disease, neuroprotection, repair and therapeutics: problems and perspectives 被引量:4
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作者 Anuradha Kalani Neetu Tyagi 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第10期1565-1567,共3页
Exosomes:Exosomes are a sub-population of micro-vesicles ranging from 40–100 nm that were earlier thought as artefacts under electron microscope.They recently came into attention for their storage of biological info... Exosomes:Exosomes are a sub-population of micro-vesicles ranging from 40–100 nm that were earlier thought as artefacts under electron microscope.They recently came into attention for their storage of biological information,cell-to-cell communication,serving as biomarkers and potential use in neural protection and regeneration (Kalani et al., 2013, 2014a). 展开更多
关键词 Exosomes in neurological disease NEUROPROTECTION problems and perspectives repair and therapeutics
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Current Situations and Problems of Investment in Agricultural Capital Construction from the Perspective of Investment of Different Ministries and Commissions and Recommendations 被引量:1
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作者 Lin ZHAI Jiliang MA 《Asian Agricultural Research》 2018年第5期1-6,9,共7页
Investment in agricultural capital construction is an important factor in improving China's agricultural productivity and international competitiveness. In recent years,the attention to the investment in agricultu... Investment in agricultural capital construction is an important factor in improving China's agricultural productivity and international competitiveness. In recent years,the attention to the investment in agricultural capital construction has been lifted to a strategic height. However,there is still little research on the investment in agricultural capital construction. From the perspective of investment of different ministries and commissions,this paper systematically analyzed current situations,problems,and came up with recommendations for the investment in the agricultural capital construction. According to the results,( i) from 1983 to the present,China's investment in agricultural capital construction experienced three stages. Especially after 2010,the scale of investment in agricultural capital construction was huge,and it showed a rapid increasing trend;( ii) the investment in agricultural capital construction is characterized by " multi-sector investment,different departments having different investment priorities and investment scale in different periods";( iii) some investment projects are overlapping between departments,local areas are weak in providing support funds,project construction standard is single,and there are problems of attaching importance to the application,belittling the management,stressing the construction,belittling the maintenance,and serious brain drain;( iv) different departments have accumulated certain experience in long-term exploration. For example,they pay more attention to the refinement and differentiation of projects,focus on the development of targeted poverty alleviation,pay more attention to the " quality" of investment,and pay more attention to the transformation of agricultural production methods. Based on current situations and problems,it came up with recommendations including promoting the integration of funds for investment and project integration,strengthening the personnel cultivation,ensuring simultaneous construction and management of projects,stressing the maintenance after project construction,exploring new modes for project design,management,investment,and financing,and enhancing the support for special investment projects. 展开更多
关键词 Investment in agricultural capital construction Current situations problemS RECOMMENDATIONS
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Problems with College Students' English Listening and Speaking and Their Solutions——from the Perspective of Sociolinguistics
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作者 刘磊 《海外英语》 2014年第23期38-39,64,共3页
This study surveyed what problems and puzzles college students have in English speaking and listening. A questionnaire survey was conducted among 157 English-major students in South-Central University for Nationalitie... This study surveyed what problems and puzzles college students have in English speaking and listening. A questionnaire survey was conducted among 157 English-major students in South-Central University for Nationalities. The result suggests that they seemed to be very anxious in communicating in English. Their problems are analyzed from the perspective of sociolinguistics and traced back to the way they are taught. This study calls for awareness of the current problems in college English listening and speaking course and provides solutions to them. 展开更多
关键词 English LISTENING and SPEAKING COURSE ANXIETY prob
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The Overseas Reception of Chinese Science Fiction Works from a Perspective of Western Interpretation Preference——A Case Study of Reader's Reception of Liu Cixin's The Three-Body Problem in the USA 被引量:1
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作者 Liu Ge Li Yun Wu Lingwei 《Contemporary Social Sciences》 2018年第6期90-102,共13页
The Three-Body Problem, written by Liu Cixin, won the 2015 Hugo Award for Best Novel, with over 200,000 copies in English already sold. This gure is hundreds of times the average sales volume of Chinese novels in the ... The Three-Body Problem, written by Liu Cixin, won the 2015 Hugo Award for Best Novel, with over 200,000 copies in English already sold. This gure is hundreds of times the average sales volume of Chinese novels in the USA. Through qualitative and quantitative analyses of overseas readers' comments on The Three-Body Problem posted on the online platforms of Goodreads and Amazon, this paper concludes that the novel's success overseas lies in its catering to the concerns of overseas readers. The ThreeBody Problem is popular among overseas readership thanks to its re ection on the ultimate existence of mankind, science ction vision & imagination, literary familiarity & identi cation, ideological di erence-triggered cultural curiosity, and a good translation. This paper attempts to interpret Liu Cixin's work on the map of world literature from the perspective of overseas readers' preferences to provide references for contemporary Chinese science ction creation and overseas reception. 展开更多
关键词 LIU CIXIN The THREE-BODY problem science ction novel OVERSEAS RECEPTION
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An efficient and globally optimal solution to perspective-n-line problem 被引量:2
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作者 Qida YU Guili XU +1 位作者 Zhengsheng WANG Zhenhua LI 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2022年第3期400-407,共8页
This research develops an accurate and efficient method for the Perspective-n-Line(Pn L)problem. The developed method addresses and solves Pn L via exploiting the problem’s geometry in a non-linear least squares fash... This research develops an accurate and efficient method for the Perspective-n-Line(Pn L)problem. The developed method addresses and solves Pn L via exploiting the problem’s geometry in a non-linear least squares fashion. Specifically, by representing the rotation matrix with a novel quaternion parameterization, the Pn L problem is first decomposed into four independent subproblems. Then, each subproblem is reformulated as an unconstrained minimization problem, in which the Kronecker product is adopted to write the cost function in a more compact form. Finally, the Groobner basis technique is used to solve the polynomial system derived from the first-order optimality conditions of the cost function. Moreover, a novel strategy is presented to improve the efficiency of the algorithm. It is improved by exploiting structure information embedded in the rotation parameterization to accelerate the computing of coefficient matrix of a cost function. Experiments on synthetic data and real images show that the developed method is comparable to or better than state-of-the-art methods in accuracy, but with reduced computational requirements. 展开更多
关键词 Camera pose estimation Grobner basis Machine vision Perspective-n-Line(PnL)problem Quaternion parameterization
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Problems of Quality of Migrant Workers and Countermeasures from the Perspective of Iceberg Model 被引量:2
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作者 YAN Yu-jie LIU Yuan 《Asian Agricultural Research》 2012年第5期48-50,共3页
From the perspective of Iceberg Model,we analyze levels and structures of quality of migrant workers from knowledge,skills,social role,self-cognition,traits and motives.On the basis of these situations,we put forward ... From the perspective of Iceberg Model,we analyze levels and structures of quality of migrant workers from knowledge,skills,social role,self-cognition,traits and motives.On the basis of these situations,we put forward countermeasures:value rural education and enhance skill training;make clear occupational planning and set up correct sense of value;assist migrant workers in facing the reality and adjusting their state of mind in working;coordinate interest relationship and call upon social care;rebuild mental world and construct harmonious society. 展开更多
关键词 Iceberg Model Quality of migrant workers problems and countermeasures
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Second-order problem solving:Nurses'perspectives on learning from.near misses 被引量:2
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作者 Yi Yang Huaping Liu Gwen D.Sherwood 《International Journal of Nursing Sciences》 CSCD 2021年第4期444-452,I0006,共10页
Objectives:Near misses happen more frequently than actual errors,and highlight system vulnerabilities without causing any harm,thus provide a safe space for organizational learning.Second-order problem solving behavio... Objectives:Near misses happen more frequently than actual errors,and highlight system vulnerabilities without causing any harm,thus provide a safe space for organizational learning.Second-order problem solving behavior offers a new perspective to better understand how nurses promote learning from near misses to improve organizational outcomes.This study aimed to explore frontline nurses’perspectives on using second-order problem solving behavior in learning from near misses to improve patient safety.Methods:A qualitative exploratory study design was employed.This study was conducted in three tertiary hospitals in east China from June to November 2015.Purposive sampling was used to recruit 19 frontline nurses.Semi-structured interviews and a qualitative directed content analysis was undertaken using Crossan’s 4I Framework of Organizational Learning as a coding framework.Results:Second-order problem solving behavior,based on the 4I Framework of Organizational Learning,was referred to as being a leader in exposing near misses,pushing forward the cause analysis within limited capacity,balancing the active and passive role during improvement project,and promoting the continuous improvement with passion while feeling low-powered.Conclusions:4I Framework of Organizational Learning can be an underlying guide to enrich frontline nurses’role in promoting organizations to learn from near misses.In this study,nurses displayed their pivotal role in organizational learning from near misses by using second-order problem solving.However,additional knowledge,skills,and support are needed to maximize the application of second-order problem solving behavior when near misses are recognized. 展开更多
关键词 Near misses Nurses Nurse’s role Patient safety problem solving Tertiary care centers 4I framework of organizational learning
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莱茵衣藻RSP10的多克隆抗体制备及应用
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作者 卢婵 张媛媛 +2 位作者 陶冶 樊振川 刘雁霞 《食品与生物技术学报》 北大核心 2025年第11期23-29,共7页
【目的】研究径向辐条蛋白RSP10如何完成纤毛组装,制备一种高特异性的RSP10多克隆抗体。【方法】首先构建原核表达载体pMal-C2X-rsp10,并在大肠杆菌BL21(DE3)中诱导表达MBP标签的RSP10,待融合蛋白纯化后切除MBP标签,免疫新西兰雄性大白... 【目的】研究径向辐条蛋白RSP10如何完成纤毛组装,制备一种高特异性的RSP10多克隆抗体。【方法】首先构建原核表达载体pMal-C2X-rsp10,并在大肠杆菌BL21(DE3)中诱导表达MBP标签的RSP10,待融合蛋白纯化后切除MBP标签,免疫新西兰雄性大白兔获得抗血清,以间接ELISA法测定其效价。【结果】通过Protein A和抗原抗体纯化后,获得了特异性强、灵敏度高的兔抗莱茵衣藻RSP10抗体,通过免疫荧光检测其定位于细胞基体部位,进一步分离纤毛组分发现RSP10大量存在于纤毛轴丝中,少量存在于纤毛基质中。【结论】该试验为后续研究RSP复合物如何完成组装及其影响纤毛运动的深层分子机理提供了有力的数据支撑。 展开更多
关键词 纤毛 rsp10 多克隆抗体 表达和纯化
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