AIM: To evaluate the treatment of pediatric functional chronic intestinal constipation (FCIC) with a probiotic goat yogurt. METHODS: A crossover double-blind formula-controlled trial was carried out on 59 students (ag...AIM: To evaluate the treatment of pediatric functional chronic intestinal constipation (FCIC) with a probiotic goat yogurt. METHODS: A crossover double-blind formula-controlled trial was carried out on 59 students (age range: 5-15 years) of a public school in Belo Horizonte, MG, Brazil, presenting a FCIC diagnostic, according to Roma Ⅲ criteria. The students were randomized in two groups to receive a goat yogurt supplemented with 109 colony forming unit/mL Bifidobacterium longum (B.longum) (probiotic) daily or only the yogurt for a period of 5 wk (formula). Afterwards, the groups were intercrossed for another 5 wk. Defecation frequency, stool consistency and abdominal and defecation pain were assessed.RESULTS: Both treatment groups demonstrated improvement in defecation frequency compared to baseline. However, the group treated with probiotic showed most signif icant improvement in the f irst phase of the study. An inversion was observed after crossing over, resulting in a reduction in stool frequency when this group was treated by formula. Probiotic and formula improved stool consistency in the f irst phase of treatment, but the improvement obtained with probiotic was significantly higher (P = 0.03). In the second phase of treatment, the group initially treated with probiotic showed worseningstool consistency when using formula. However, the difference was not signif icant. A signif icant improvement in abdominal pain and defecation pain was observed with both probiotic and formula in the first phase of treatment, but again the improvement was more signif icant for the group treated with B. longum during phase I (P < 0.05). When all data of the crossover study were analyzed, significant differences were observed between probiotic yogurt and yogurt only for defecation frequency (P = 0.012), defecation pain (P = 0.046) and abdominal pain (P = 0.015).展开更多
Lactiplantibacillus plantarum Y42 exhibited good bioactivity in our previous study.In this study,the whole genome of L.plantarum Y42 was sequenced and its genome sequence information and function were analyzed to inve...Lactiplantibacillus plantarum Y42 exhibited good bioactivity in our previous study.In this study,the whole genome of L.plantarum Y42 was sequenced and its genome sequence information and function were analyzed to investigate its genotype and possible probiotic qualities.Thegenome of L.plantarum Y42 consisted of a circular 3.32 M bp chromosome,1 linear plasmid(0.013 M bp)and 1 ring-shaped plasmid(0.054 M bp),and contained a variety of genes associated with beneficial functions such as bacterial adhesion,acid tolerance,bile salt tolerance and heat tolerance.In particular,five genes associated with quorum sensing(QS)system(LuxS,Pfs,agrA,agrB,ftsH),three complete extracellular polysaccharide(EPS)synthesis gene clusters(cps2,cps3,cps4)and a cluster of pln genes encoding bacteriocins,which contains 21 genes,were identified in the L.plantarum Y42 genome.These results reveal the potential probiotic mechanism of L.plantarum Y42 at the gene level,and theoretically underlie its probiotic function excavation and practical application.展开更多
基金Supported by Grants from Conselho Nacional de Desenvolvi-mento Cientifico e TecnológicoFundaco de Amparo à Pesquisa do Estado de Minas Gerais
文摘AIM: To evaluate the treatment of pediatric functional chronic intestinal constipation (FCIC) with a probiotic goat yogurt. METHODS: A crossover double-blind formula-controlled trial was carried out on 59 students (age range: 5-15 years) of a public school in Belo Horizonte, MG, Brazil, presenting a FCIC diagnostic, according to Roma Ⅲ criteria. The students were randomized in two groups to receive a goat yogurt supplemented with 109 colony forming unit/mL Bifidobacterium longum (B.longum) (probiotic) daily or only the yogurt for a period of 5 wk (formula). Afterwards, the groups were intercrossed for another 5 wk. Defecation frequency, stool consistency and abdominal and defecation pain were assessed.RESULTS: Both treatment groups demonstrated improvement in defecation frequency compared to baseline. However, the group treated with probiotic showed most signif icant improvement in the f irst phase of the study. An inversion was observed after crossing over, resulting in a reduction in stool frequency when this group was treated by formula. Probiotic and formula improved stool consistency in the f irst phase of treatment, but the improvement obtained with probiotic was significantly higher (P = 0.03). In the second phase of treatment, the group initially treated with probiotic showed worseningstool consistency when using formula. However, the difference was not signif icant. A signif icant improvement in abdominal pain and defecation pain was observed with both probiotic and formula in the first phase of treatment, but again the improvement was more signif icant for the group treated with B. longum during phase I (P < 0.05). When all data of the crossover study were analyzed, significant differences were observed between probiotic yogurt and yogurt only for defecation frequency (P = 0.012), defecation pain (P = 0.046) and abdominal pain (P = 0.015).
基金supported by the National Natural Science Foundation of China(32072193).
文摘Lactiplantibacillus plantarum Y42 exhibited good bioactivity in our previous study.In this study,the whole genome of L.plantarum Y42 was sequenced and its genome sequence information and function were analyzed to investigate its genotype and possible probiotic qualities.Thegenome of L.plantarum Y42 consisted of a circular 3.32 M bp chromosome,1 linear plasmid(0.013 M bp)and 1 ring-shaped plasmid(0.054 M bp),and contained a variety of genes associated with beneficial functions such as bacterial adhesion,acid tolerance,bile salt tolerance and heat tolerance.In particular,five genes associated with quorum sensing(QS)system(LuxS,Pfs,agrA,agrB,ftsH),three complete extracellular polysaccharide(EPS)synthesis gene clusters(cps2,cps3,cps4)and a cluster of pln genes encoding bacteriocins,which contains 21 genes,were identified in the L.plantarum Y42 genome.These results reveal the potential probiotic mechanism of L.plantarum Y42 at the gene level,and theoretically underlie its probiotic function excavation and practical application.
