Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiatio...Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. Using probes that target specific regions on chromosomes associated with a distinct subset of microdeletions and microduplications either established or thought to be responsible for intellectual disability or developmental delay, we have demonstrated that WTK1 and TK6 are not impacted in the same way by irradiation. Instead, each cell line presents its own unique MLPA profile. The most notable differences are the appearance of nine unique probe signals only seen in WTK1 cells. These results are important in the study of how different cell lines can be affected in significantly different ways depending on the presence or absence of wild type p53.展开更多
High pressure, radon bearing water has been identified as one of the most critical challenges in mining the high-grade uranium deposit at the Mc Arthur River Operation, Cameco Corporation. The ore deposits are located...High pressure, radon bearing water has been identified as one of the most critical challenges in mining the high-grade uranium deposit at the Mc Arthur River Operation, Cameco Corporation. The ore deposits are located between 490 and 640 m below the surface and surrounded by water bearing Athabasca sandstone, a graphitic P2 fault zone, and highly altered ground. This paper introduces the inflow risk management program at Mc Arthur River Operation, which includes various hydrogeological challenges and the corresponding strategies applied, such as risk-based probe and grout programs(geological, hydrogeological, and geotechnical), ground freezing programs, and comprehensive ground control programs. These programs have been developed, tested, and proven successful over years of mining practices. Working with this world class deposit of high risk and low tolerance, it is believed that these experiences might be beneficial to other mining operations with similar hydrogeological characteristics.展开更多
文摘Multiplex Ligation-Dependent Probe Amplification (MLPA) was used to study the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. Using probes that target specific regions on chromosomes associated with a distinct subset of microdeletions and microduplications either established or thought to be responsible for intellectual disability or developmental delay, we have demonstrated that WTK1 and TK6 are not impacted in the same way by irradiation. Instead, each cell line presents its own unique MLPA profile. The most notable differences are the appearance of nine unique probe signals only seen in WTK1 cells. These results are important in the study of how different cell lines can be affected in significantly different ways depending on the presence or absence of wild type p53.
文摘High pressure, radon bearing water has been identified as one of the most critical challenges in mining the high-grade uranium deposit at the Mc Arthur River Operation, Cameco Corporation. The ore deposits are located between 490 and 640 m below the surface and surrounded by water bearing Athabasca sandstone, a graphitic P2 fault zone, and highly altered ground. This paper introduces the inflow risk management program at Mc Arthur River Operation, which includes various hydrogeological challenges and the corresponding strategies applied, such as risk-based probe and grout programs(geological, hydrogeological, and geotechnical), ground freezing programs, and comprehensive ground control programs. These programs have been developed, tested, and proven successful over years of mining practices. Working with this world class deposit of high risk and low tolerance, it is believed that these experiences might be beneficial to other mining operations with similar hydrogeological characteristics.
