Objective:To establish a new detecting method for disease susceptibility loci R1628P and G2385R of Parkinson’s disease(PD)related gene LRRK2.Methods:Sequence specific primers were designed to make a genotyping of DNA...Objective:To establish a new detecting method for disease susceptibility loci R1628P and G2385R of Parkinson’s disease(PD)related gene LRRK2.Methods:Sequence specific primers were designed to make a genotyping of DNA markers with known genotypes by use of quantitative fluorescence real-time PCR(RT-PCR).100 cases of PD samples with unknown genotypes were tested,and verified by use of polymerase chain reaction linked restriction fragment length polymorphism(PCR-RLFP).Results:The genotyping results of DNA markers proved to be correct,and 100 cases of samples to be tested had a completely consistent genotyping result with PCR-RLFP genotyping result.Conclusions:Sequence specific primer and quantitative fluorescence RT-PCR can successfully make a genotyping for disease susceptibility loci R1628P and G2385R of LRRK2.展开更多
The authors regret that the qPCR primer sequence and Supplemen-tary Fig.S50 in supplementary data are incorrect.After reviewing our published work,we realized that the primer sequence for Bcl2 was written incorrectly ...The authors regret that the qPCR primer sequence and Supplemen-tary Fig.S50 in supplementary data are incorrect.After reviewing our published work,we realized that the primer sequence for Bcl2 was written incorrectly due to copy and paste during the writing process.And the primer sequence for Actin was omitted.In Supplementary Fig.S50,the H&E staining image corresponding to IV group was incorrectly provided.The corrected qPCR primer sequence and Fig.S50 is provided in this corrigendum.The authors are sorry for not checking the supplementary documents carefully before publication。展开更多
文摘Objective:To establish a new detecting method for disease susceptibility loci R1628P and G2385R of Parkinson’s disease(PD)related gene LRRK2.Methods:Sequence specific primers were designed to make a genotyping of DNA markers with known genotypes by use of quantitative fluorescence real-time PCR(RT-PCR).100 cases of PD samples with unknown genotypes were tested,and verified by use of polymerase chain reaction linked restriction fragment length polymorphism(PCR-RLFP).Results:The genotyping results of DNA markers proved to be correct,and 100 cases of samples to be tested had a completely consistent genotyping result with PCR-RLFP genotyping result.Conclusions:Sequence specific primer and quantitative fluorescence RT-PCR can successfully make a genotyping for disease susceptibility loci R1628P and G2385R of LRRK2.
文摘The authors regret that the qPCR primer sequence and Supplemen-tary Fig.S50 in supplementary data are incorrect.After reviewing our published work,we realized that the primer sequence for Bcl2 was written incorrectly due to copy and paste during the writing process.And the primer sequence for Actin was omitted.In Supplementary Fig.S50,the H&E staining image corresponding to IV group was incorrectly provided.The corrected qPCR primer sequence and Fig.S50 is provided in this corrigendum.The authors are sorry for not checking the supplementary documents carefully before publication。
