Mus musculus tyrosine 3-monooxygenase/trytophan 5-monooxygenase activation protein,zeta polypeptide(Ywhaz)is related to communication among blastomeres and con-tributes to buildup of celiular communication system,and ...Mus musculus tyrosine 3-monooxygenase/trytophan 5-monooxygenase activation protein,zeta polypeptide(Ywhaz)is related to communication among blastomeres and con-tributes to buildup of celiular communication system,and ATPase 6 is essential for one-cell to two-cell transition and play an important role in establishment of oxidative phosphorylation.We report the expression analysis of mouse two-celi stage specific genes using modified mR-NA differential display.The results of sequence analysisand reverse northern blot indicate that the genes for ATPase 6and Ywhaz are expressed just in 2-cell embryo.展开更多
Transposable elements,long considered genomic intruders,have been found to play significant and intriguing roles during early embryonic development based on the paradigm shift that has undergone in recent years.Long i...Transposable elements,long considered genomic intruders,have been found to play significant and intriguing roles during early embryonic development based on the paradigm shift that has undergone in recent years.Long interspersed element-1(LINE-1)is the predominant class of retrotransposons with autonomous retrotransposition capabilities in mammals and has emerged as a crucial element of preimplantation development.In this review,we elucidate the expression dynamics of key transposable elements throughout preimplantation development and their contribution to the regulation of developmental progression and totipotency.We also explore the critical function of LINE-1 activation and its rich functional reservoir,which is exploited by the host to provide cis-regulatory elements and functional proteins.Particular highlights of the widespread activities in preimplantation development of LINE-1 during multiple epigenetic modifications such as DNA methylation,histone methylation,ubiquitination,and RNA methylation.The silencing complex and RNA exosome also coordinate with LINE-1 across distinct developmental stages.Accordingly,the up-regulated expression of LINE-1 retrotransposons and their protein products plays a key role in various processes,including the opening of chromatin architecture,zygotic genome activation,aging,and age-related disorders.It may reflect an effect on totipotency and pluripotency of mammalian development.Underscoring its pivotal significance,the nuanced regulation of LINE-1 illuminates its indispensable role in orchestrating the precise coordination essential for the regulation of cellular pluripotency and the intricate mechanisms of zygotic genome activation.展开更多
Objective To explore the effect of hepatocyte growth factor （HGF） and fibroblast growth factor （FGF） on preimplantation embryo development of mouse.Methods Mated mice were killed by cervical dislocation to collect...Objective To explore the effect of hepatocyte growth factor （HGF） and fibroblast growth factor （FGF） on preimplantation embryo development of mouse.Methods Mated mice were killed by cervical dislocation to collect two pronucleous （2 PN） zygotes from oviduct of pregnant 1 d NMRI mice and were cultured to the hatching blastocyst stage and the number of embryo in different stages was recorded under an invert microscope. The cleavage rates of formed 2 PN zygotes were compared with blastocyst and hatching blastocyst stage in drops of T6 medium with or without HGF or FGF （0 ng/ml, 10 ng/ml, 20 ng/ml, 50 ng/ml, 1 000 ng/ml）. Results HGF and FGF enriched embryo culture media promotea the aevetopment from 2 PN stage embryos to blastocyst. Adding 20 ng/ml of FGF or HGF to the culture medium significantly increased the percentage of 2PN embryos that developed into blastocysts （P〈0.05）, but culture of embryos in drops of T6 medium with HGF （20 ng/ml） had no improvement in in-vitro hatching.Conclusion Exogenous HGF and FGF at low concentrations promote in-vitro mouse blastocyst formation.展开更多
Cannabinoids have long been suspected associating with abnormal fetal growth and outcome. However, the molecular mechanisms in which they are involved had long been obscure for several decades. Only recently, after th...Cannabinoids have long been suspected associating with abnormal fetal growth and outcome. However, the molecular mechanisms in which they are involved had long been obscure for several decades. Only recently, after the identification of two types of cannabinoid receptors （CB1-R and CB2-R） and the following discoveries of their corresponding endogenous ligands, the mystery behind those seemingly facts began gradually unveiled. Through a series of landmark research, it is now indicated that the endocannabinoid signaling via the ligand-receptor interaction plays an important role in modulating early development of preimplantation embryo and synchronizing embryo development with uterine receptivity for implantation. Current data suggest that the physiological functions their metabolic pathways are potentially very of endocannabinoid signaling as well as exited areas to be explored further. This review will first introduce the reproductive functions of endocannabinoid signaling from epidemiological and molecular background, then focus on its reciprocal interactions between the embryo and maternal tissues, as well as related metabolic aspects in regards to implantation. It is hoped that further investigation of this physiologically fundamental signaling will generate more exciting information elucidating the complexity of implantation thus lead to a better control of human reproduction.展开更多
Objective To investigate the effect of the glucose-free preimplantation stage one （P1） medium and the ECM medium on embryo development quality in IVF. Methods The patients with ≥4 zygotes of 2PN were studied. A tot...Objective To investigate the effect of the glucose-free preimplantation stage one （P1） medium and the ECM medium on embryo development quality in IVF. Methods The patients with ≥4 zygotes of 2PN were studied. A total of 201 retrieval cycles were included in a prospective randomized study. Each patient was herself control. Half of zygotes of 2PN were transferred into ECM medium （group A） and half into P1 medium （group B）for further culture. Embryo development was evaluated on the day of embryo transfer. The efficacy of ECM was compared with P1 as culture medium for the development of preimplantation embryos. Results No statistically significant differences were noted between the two groups regarding embryo-cleavage rate （97.13% vs 97.55%） and rate of normal-cleaving embryos （58.29% and 58.37%）. The rate of top-quality embryos was statistically higher in group A than in group B （27.59% vs 19.75%, P〈O.05）. Embryo quality, as assessed by morphological parameters （the amount of detached anuclear fragments 〉30%）, was better in group A than in group B （19.86% vs 21.75%）, however, there was no statistically significance. Both the rate of good-quality embryos （47.95% vs 46.17%） and available embryos （63.22% vs 61.19%） were higher in group A than in group B, but there was also no statistically significance. Conclusion The ECM medium may be associated with a better embryo quality compared with the P1 medium.展开更多
Polycomb group(Pc G)proteins are crucial chromatin regulators during development.H2 AK119 ub1(H2 Aub)and H3 K27 me3 are catalyzed by Polycomb-repressive complex 1 and 2(PRC1/2)respectively,and they largely overlap in ...Polycomb group(Pc G)proteins are crucial chromatin regulators during development.H2 AK119 ub1(H2 Aub)and H3 K27 me3 are catalyzed by Polycomb-repressive complex 1 and 2(PRC1/2)respectively,and they largely overlap in the genome due to mutual recruitment of the two complexes.However,it is unclear whether PRC1/H2 Aub and PRC2/H3 K27 me3 can also function independently.By developing an ultra-sensitive carrier-DNA-assisted chromatin immunoprecipitation sequencing method termed CATCH-Seq,we generated allelic H2 Aub profiles in mouse gametes and early embryos.Our results revealed an unexpected genomewide decoupling of H2 Aub and H3 K27 me3 in mouse preimplantation embryos,where H2 Aub but not H3 K27 me3 was enriched at Pc G targets while only H3 K27 me3 was deposited in the broad distal domains associated with DNA methylation-independent non-canonical imprinting.These observations suggest that H2 Aub represses future bivalent genes during early embryogenesis without H3 K27 me3,but it is not required for the maintenance of non-canonical imprinting,which is mediated by maternal H3 K27 me3.Thus,our study reveals the distinct depositions and independent functions of H2 Aub and H3 K27 me3 during early mammalian development.展开更多
基金The project was supported by the State Basic Research Development Program of“973”(No.G200016107)
文摘Mus musculus tyrosine 3-monooxygenase/trytophan 5-monooxygenase activation protein,zeta polypeptide(Ywhaz)is related to communication among blastomeres and con-tributes to buildup of celiular communication system,and ATPase 6 is essential for one-cell to two-cell transition and play an important role in establishment of oxidative phosphorylation.We report the expression analysis of mouse two-celi stage specific genes using modified mR-NA differential display.The results of sequence analysisand reverse northern blot indicate that the genes for ATPase 6and Ywhaz are expressed just in 2-cell embryo.
基金supported by grants from the National Natural Science Foundation of China(No.32371217,32070860)the National Key R&D Plan of China(No.2021YFA1101002)+1 种基金Tianjin Natural Science Foundation(China)(No.22JCYBJC01220)the National Students'Platform for Innovation and Entrepreneurship Training Program(China)(No.202410055320,202410055676).
文摘Transposable elements,long considered genomic intruders,have been found to play significant and intriguing roles during early embryonic development based on the paradigm shift that has undergone in recent years.Long interspersed element-1(LINE-1)is the predominant class of retrotransposons with autonomous retrotransposition capabilities in mammals and has emerged as a crucial element of preimplantation development.In this review,we elucidate the expression dynamics of key transposable elements throughout preimplantation development and their contribution to the regulation of developmental progression and totipotency.We also explore the critical function of LINE-1 activation and its rich functional reservoir,which is exploited by the host to provide cis-regulatory elements and functional proteins.Particular highlights of the widespread activities in preimplantation development of LINE-1 during multiple epigenetic modifications such as DNA methylation,histone methylation,ubiquitination,and RNA methylation.The silencing complex and RNA exosome also coordinate with LINE-1 across distinct developmental stages.Accordingly,the up-regulated expression of LINE-1 retrotransposons and their protein products plays a key role in various processes,including the opening of chromatin architecture,zygotic genome activation,aging,and age-related disorders.It may reflect an effect on totipotency and pluripotency of mammalian development.Underscoring its pivotal significance,the nuanced regulation of LINE-1 illuminates its indispensable role in orchestrating the precise coordination essential for the regulation of cellular pluripotency and the intricate mechanisms of zygotic genome activation.
文摘Objective To explore the effect of hepatocyte growth factor （HGF） and fibroblast growth factor （FGF） on preimplantation embryo development of mouse.Methods Mated mice were killed by cervical dislocation to collect two pronucleous （2 PN） zygotes from oviduct of pregnant 1 d NMRI mice and were cultured to the hatching blastocyst stage and the number of embryo in different stages was recorded under an invert microscope. The cleavage rates of formed 2 PN zygotes were compared with blastocyst and hatching blastocyst stage in drops of T6 medium with or without HGF or FGF （0 ng/ml, 10 ng/ml, 20 ng/ml, 50 ng/ml, 1 000 ng/ml）. Results HGF and FGF enriched embryo culture media promotea the aevetopment from 2 PN stage embryos to blastocyst. Adding 20 ng/ml of FGF or HGF to the culture medium significantly increased the percentage of 2PN embryos that developed into blastocysts （P〈0.05）, but culture of embryos in drops of T6 medium with HGF （20 ng/ml） had no improvement in in-vitro hatching.Conclusion Exogenous HGF and FGF at low concentrations promote in-vitro mouse blastocyst formation.
基金This study was supported by grants from National Natural Science Fundation of China(No.30470654)
文摘Cannabinoids have long been suspected associating with abnormal fetal growth and outcome. However, the molecular mechanisms in which they are involved had long been obscure for several decades. Only recently, after the identification of two types of cannabinoid receptors （CB1-R and CB2-R） and the following discoveries of their corresponding endogenous ligands, the mystery behind those seemingly facts began gradually unveiled. Through a series of landmark research, it is now indicated that the endocannabinoid signaling via the ligand-receptor interaction plays an important role in modulating early development of preimplantation embryo and synchronizing embryo development with uterine receptivity for implantation. Current data suggest that the physiological functions their metabolic pathways are potentially very of endocannabinoid signaling as well as exited areas to be explored further. This review will first introduce the reproductive functions of endocannabinoid signaling from epidemiological and molecular background, then focus on its reciprocal interactions between the embryo and maternal tissues, as well as related metabolic aspects in regards to implantation. It is hoped that further investigation of this physiologically fundamental signaling will generate more exciting information elucidating the complexity of implantation thus lead to a better control of human reproduction.
文摘Objective To investigate the effect of the glucose-free preimplantation stage one （P1） medium and the ECM medium on embryo development quality in IVF. Methods The patients with ≥4 zygotes of 2PN were studied. A total of 201 retrieval cycles were included in a prospective randomized study. Each patient was herself control. Half of zygotes of 2PN were transferred into ECM medium （group A） and half into P1 medium （group B）for further culture. Embryo development was evaluated on the day of embryo transfer. The efficacy of ECM was compared with P1 as culture medium for the development of preimplantation embryos. Results No statistically significant differences were noted between the two groups regarding embryo-cleavage rate （97.13% vs 97.55%） and rate of normal-cleaving embryos （58.29% and 58.37%）. The rate of top-quality embryos was statistically higher in group A than in group B （27.59% vs 19.75%, P〈O.05）. Embryo quality, as assessed by morphological parameters （the amount of detached anuclear fragments 〉30%）, was better in group A than in group B （19.86% vs 21.75%）, however, there was no statistically significance. Both the rate of good-quality embryos （47.95% vs 46.17%） and available embryos （63.22% vs 61.19%） were higher in group A than in group B, but there was also no statistically significance. Conclusion The ECM medium may be associated with a better embryo quality compared with the P1 medium.
基金supported by the National Natural Science Foundation of China(32022023 and 31871478)the National Key Research and Development Programs of China(2017YFC1001500)Zhejiang Provincial Natural Science Foundation of China(LR18C060001)。
文摘Polycomb group(Pc G)proteins are crucial chromatin regulators during development.H2 AK119 ub1(H2 Aub)and H3 K27 me3 are catalyzed by Polycomb-repressive complex 1 and 2(PRC1/2)respectively,and they largely overlap in the genome due to mutual recruitment of the two complexes.However,it is unclear whether PRC1/H2 Aub and PRC2/H3 K27 me3 can also function independently.By developing an ultra-sensitive carrier-DNA-assisted chromatin immunoprecipitation sequencing method termed CATCH-Seq,we generated allelic H2 Aub profiles in mouse gametes and early embryos.Our results revealed an unexpected genomewide decoupling of H2 Aub and H3 K27 me3 in mouse preimplantation embryos,where H2 Aub but not H3 K27 me3 was enriched at Pc G targets while only H3 K27 me3 was deposited in the broad distal domains associated with DNA methylation-independent non-canonical imprinting.These observations suggest that H2 Aub represses future bivalent genes during early embryogenesis without H3 K27 me3,but it is not required for the maintenance of non-canonical imprinting,which is mediated by maternal H3 K27 me3.Thus,our study reveals the distinct depositions and independent functions of H2 Aub and H3 K27 me3 during early mammalian development.
