Preformed albumin corona of albumin-nonselective nanoparticles(NPs)is widely exploited to inhibit the unavoidable protein adsorption upon intravenous administration.However,very few studies have concerned the preforme...Preformed albumin corona of albumin-nonselective nanoparticles(NPs)is widely exploited to inhibit the unavoidable protein adsorption upon intravenous administration.However,very few studies have concerned the preformed albumin corona of albumin-selective NPs.Herein,we report a novel type of albumin-selective NPs by decorating 6-maleimidocaproyl polyethylene glycol stearate(SA)onto PLGA NPs(SP NPs)surface,taking albuminnonselective PLGA NPs as control.PLGA NPs and SP NPs were prepared by emulsion-solvent evaporation method and the resultant NPs were in spherical shape with an average diameter around 180 nm.The corresponding albumin-coating PLGA NPs(PLGA@BSA NPs)and albumin-coating SP NPs(SP@BSA NPs)were formulated by incubating SP NPs or PLGA NPs with bovine serum albumin solution,respectively.The impact of albumin corona on particle characteristics,stability,photothermal effect,cytotoxicity,cell uptake,spheroid penetration and pharmacokinetics was investigated.In line with previous findings of preformed albumin coating,PLGA@BSA NPs exhibited higher stability,cytotoxicity,cell internalization and spheroid penetration performances in vitro,and longer blood circulation time in vivo than those of albumin-nonselective PLGA NPs,but albumin-selective SP NPs is capable of achieving a comparable in vitro and in vivo performances with both SP@BSA NPs and PLGA@BSA NPs.Our results demonstrate that SA decorated albumin-selective NPs pave a versatile avenue for optimizing nanoparticulate delivery without preformed albumin corona.展开更多
The development of unconventional petroleum resources has gradually become an important succession for increasing oil production.However,the related engineers and researchers are paying more and more attention to the ...The development of unconventional petroleum resources has gradually become an important succession for increasing oil production.However,the related engineers and researchers are paying more and more attention to the application of temporary plugging agents(TPAs)for their efficient development.TPAs can expand the stimulated reservoir volume(SRV)and facilitate the flow of oil and gas to the bottom of the well.Particle-gels used as temporary plugging agents have the characteristics of the simple injection process,good deformation,high plugging strength,and complete self-degradation performance,which have been widely applied in recent years.In this paper,five samples of DPPG polymerized by different molecular weights of cross-linking agents were prepared.In addition,infrared spectroscopy analysis,differential calorimetry scanning(DSC)analysis,static particle gel swelling and degradation performance evaluation experiments,and dynamic temporary plugging performance experiments in cores were conducted at 34°C.Results show that as the molecular weight of the cross-linking agent(at 0.01 g)in the DPPG molecule decreased from 1,000 to 200 Da,the fewer cross-linking sites of DPPG,the looser the microscopic three-dimensional mesh structure formed.The swelling ratio increased from 7 to 33 times.However,the complete degradation time increased from 40 to 210 min.Moreover,the DSC results confirmed that the higher the molecular weight of the cross-linking agent,the worse is chemical stability and the more prone it to self-degradation.DPPG samples had good temporary plugging performance in reservoir cores.DPPGs prepared by the cross-linking agent with smaller molecular weight has a stronger swelling ratio,higher gel strength,and greater plugging strength in the core permeabilities.Moreover,the degraded DPPG is less damaging to the cores.However,their slower degradation rates take a slightly longer times to reach complete degradation.The results of this paper can provide new ideas and a theoretical basis for the development of particle gel-type temporary plugging agents(TPA)with controllable degradation time in low-temperature reservoirs.It can help to expand the application range of existing DPPG reservoir conditions.展开更多
The paper discusses the difference in the formulation concepts of alumina - spinel （spinel containing ） and alumina - magnesia （ spin, el forming） castables and the influence on their physical properties. The indi...The paper discusses the difference in the formulation concepts of alumina - spinel （spinel containing ） and alumina - magnesia （ spin, el forming） castables and the influence on their physical properties. The individual property profile is discussed with respect to the requirements of refactory lining materials for the different zones of a steel ladle.展开更多
Model of hole-pairs in electrical transport along ab plane in cuprate superconductors has already been proposed. It has been found to be in the shape of 3dx2–y2 orbital of an electron in an atom. This time, model of ...Model of hole-pairs in electrical transport along ab plane in cuprate superconductors has already been proposed. It has been found to be in the shape of 3dx2–y2 orbital of an electron in an atom. This time, model of hole-pairs in transport along c-axis in cuprate superconductors is proposed. In ab-plane, hole-pairs are formed along CuO2 plane;one hole-pair covering 9 - 10 two dimensional CuO2 unit cells in 3dx2–y2 configuration. In the investigation of c-axis hole-pairs, cuprate superconductors have been sub-divided into three categories depending on the number of CuO2 planes/formula unit. There is a little different treatment for finding out the order parameter in each category. Coherence lengths along ab-planes are of the order of a few tens of Angstroms, whereas along c-axis, they are less than even their a-, b-lattice constants. In cuprates with 2 or 3 CuO2 planes, the order parameter is of 3dz2–x2 type in zx-plane with lobes along both the axes much constrained. For cuprates with a single CuO2 layer, the order parameter is of 3dx2–y2 type, but its dimensions are less than a-, b-lattice constants.展开更多
The reactivity of human preformed natural antibodies (PNAbs) with various porcine pancreatic cells and its isotypes was investigated. Eighteen serum samples from patients with insulin dependent diabetes mellitus (IDD...The reactivity of human preformed natural antibodies (PNAbs) with various porcine pancreatic cells and its isotypes was investigated. Eighteen serum samples from patients with insulin dependent diabetes mellitus (IDDM) and 20 serum samples from healthy human subjects were collected. The frozen sections of the pig pancreas were incubated with these sera, and subsequently incubated with FITC conjugated goat antihuman IgG and IgM monoclonal antibodies. The reactivity of human PNAbs with various porcine pancreatic cells was determined by indirect immunofluorescence staining technique. The results showed that 55.6 % of IDDM patients and 55.0 % of healthy human individuals contained PNAbs against porcine endocrine cells. However, the percentage of strongly reacting sera in the patient group was significantly increased as compared with that in the control group. All used sera from IDDM patients and 95 % of sera from healthy controls could react to one or more of the various pancreatic cell types, including: endocrine cells, exocrine cells, vascular endothelial cells, ductal epithelial cells and macrophages. The isotypes of PNAbs contained both IgG and IgM. In view of strongly positive reactivity of PNAbs with various porcine pancreatic cells, pretransplantly cross matching test and graft pretreatment may be necessary for survival of islet transplants.展开更多
A model of preformed hole-pairs in cuprate superconductors has been proposed based on some experimental results i.e., 1) electron paramagnetic resonance spectra of quenched superconductors which show very frequently t...A model of preformed hole-pairs in cuprate superconductors has been proposed based on some experimental results i.e., 1) electron paramagnetic resonance spectra of quenched superconductors which show very frequently the fragment (CuO)4 broken off from the CuO2 layer in the structure, 2) 41 meV peak observed in neutron diffraction and nuclear magnetic resonance spectra of superconductors, 3) Heisenberg exchange interaction leading to ferromagnetism observed in CuO which is an essential ingredient of all superconductors and some generally accepted conclusions i.e., a) that the order parameter in superconductors has dx2–y2 symmetr and b) coherence length is of the order of 15 - 20 Angstrom. Heisenberg exchange interaction between two (CuO4) plaquettes each containing a lattice hole binds the two holes which are the charge carriers in the cuprate superconductors. It is not very clear whether the hole-pair is in the triplet or singlet state, but the triplet state is supported by the experimental observation of ferromagnetism in the parent material CuO. The proposed hole-pair singlet is different from Zhang-Rice singlet.展开更多
To understand the applicability of high-temperature preformed particle gel(HT-PPG)for control of short-circuiting in enhanced geothermal systems(EGSs),core flooding experiments were conducted on fractured granite core...To understand the applicability of high-temperature preformed particle gel(HT-PPG)for control of short-circuiting in enhanced geothermal systems(EGSs),core flooding experiments were conducted on fractured granite cores under varying fracture widths,gel particle sizes and swelling ratios.Key parameters such as injection pressure,water breakthrough pressure,and residual resistance factor were measured to evaluate HT-PPG performance.The gel exhibited strong injectability,entering granite fractures at pressure gradients as low as 0.656 MPa/m;HT-PPG yields a superior sealing performance by significantly reducing the permeability;and dehydration occurs during HT-PPG propagation,with a dehydration ratio ranging from 4.71%to 11.36%.This study reveals that HT-PPG can be injected into geothermal formations with minimal pressure yet provides strong resistance to breakthrough once in place.This balance of injectability and sealing strength makes HT-PPG effective for addressing thermal short-circuiting in EGS reservoirs.展开更多
Re-crosslinkable preformed particle gel(RPPG)has been considered to be one of the most promising gels for dealing with fracture and void space conduit(VSC)conformance problems.However,the dehydration of RPPG during it...Re-crosslinkable preformed particle gel(RPPG)has been considered to be one of the most promising gels for dealing with fracture and void space conduit(VSC)conformance problems.However,the dehydration of RPPG during its propagation in the fracture-type features and its effect on gel properties remains unclear.This paper investigates the dehydration behavior during RPPG propagating in an open fracture using matrix-free fracture model.Then the results were verified using real fractured sandstone core model.Moreover,the gel properties after extruding a fracture were studied in detail including gel dehydration and gel strength.Results reveal that the RPPG properties changed significantly with increasing propagation distance,which correlated with the gel injection rate.At high gel injection rates,the dehydration and gel strength(Gʹ)decrease with increasing propagation distance.In contrast,the opposite result was found at low injection rates.Based on the study of the different gel injection rates,it is found that dehydration time is another key factor affecting dehydration behavior.Results also indicate that the fracture width affects gel dehydration at different locations.Dehydration was more pronounced at narrow fractures but only in the inlet section,while in the outlet section,RPPG contains more water than the initial condition.This study has profound implications for field applications.It provides new insights into the transport of RPPG in fractures and helps field engineers to optimize the gel injection operations.展开更多
The therapeutic interventions of human hypertrophic scars(HHS)remain puzzle largely due to the lack of accepted models.Current HHS models are limited by their inability to mimic native scar architecture and associated...The therapeutic interventions of human hypertrophic scars(HHS)remain puzzle largely due to the lack of accepted models.Current HHS models are limited by their inability to mimic native scar architecture and associated pathological microenvironments.Here,we create a 3D functional HHS model by preformed cellular aggregates(PCA)bioprinting,firstly developing bioink from scar decellularized extracellular matrix(ECM)and alginate-gelatin(Alg-Gel)hydrogel with suitable physical properties to mimic the microenvironmental factors,then pre-culturing patient-derived fibroblasts in this bioink to preform the topographic cellular aggregates for sequent printing.We confirm the cell aggregates preformed in bioink displayed well defined aligned structure and formed functional scar tissue self-organization after bioprinting,hence showing the potential of creating HHS models.Notably,these HHS models exhibit characteristics of early-stage HHS in gene and protein expression,which significantly activated signaling pathway related to inflammation and cell proliferation,and recapitulate in vivo tissue dynamics of scar forming.We also use the in vitro and in vivo models to define the clinically observed effects to treatment with concurrent anti-scarring drugs,and the data show that it can be used to evaluate the potential therapeutic target for drug testing.The ideal humanized scar models we present should prove useful for studying critical mechanisms underlying HHS and to rapidly test new drug targets and develop patient-specific optimal therapeutic strategies in the future.展开更多
The acidic strength of ordered mesoporous aluminosilicates of MAS-2, MAS-3, MAS-7 and MAS-9 and mi-croporous crystals of Y, L, beta, and ZSM-5 zeolites was systemically investigated by temperature-programmed desorptio...The acidic strength of ordered mesoporous aluminosilicates of MAS-2, MAS-3, MAS-7 and MAS-9 and mi-croporous crystals of Y, L, beta, and ZSM-5 zeolites was systemically investigated by temperature-programmed desorption of ammonia (NH3-TPD). Due to the use of preformed zeolite precursors of Y, L, beta and ZSM-5, the ordered mesoporous aluminosilicates with distinguished acidic strength were obtained, being dependent on the type of preformed zeolite precursors. Therefore, the acidic strength of these mesoporous aluminosilicates could be tai-lored and controlled.展开更多
Inspired by the viscoelastic displacement theory,a product called preformed particle gel(PPG)is developed as conformance control agent to enhance oil recovery and control excess water production.The migration law of P...Inspired by the viscoelastic displacement theory,a product called preformed particle gel(PPG)is developed as conformance control agent to enhance oil recovery and control excess water production.The migration law of PPG suspension in porous media is related to its deep profile control and displacement capability.Laboratory experiments indicate that PPG suspension has good viscosity increasing,and the apparent viscosity decreases with the increase of shear rate.PPG suspension is mainly elastic,and its network structure makes it have certain shear stability.PPG particles realize migration in porous media in the way of“accumulation and blockage/pressure increase/deformation and migration”.When the ratio of the PPG particle size to the pore throat diameter d ranges from 35.52 to 53.38,the particles can match through the porous medium.When the permeability difference of the parallel model is 5,PPG suspension has the highest profile improvement rate,69.10%.PPG suspension can adjust the planar heterogeneity,and increase the oil recovery rate by 20.75%.The PPG suspension can effectively start“cluster"、“film”and“blind end residual oil”,and has a high oil washing efficiency.The core NMR T2 spectrum shows that PPG suspension mainly reduces oil saturation in mesopores and macropores.After PPG flooding,the EOR capacity of small pores is the highest,39.11%.展开更多
By combining experimental α-decay energies and half-lives, the α-particle preformation factor for nuclei around neutron magic numbers N of 126, 152, and 162 were extracted using the two-potential approach. The nucle...By combining experimental α-decay energies and half-lives, the α-particle preformation factor for nuclei around neutron magic numbers N of 126, 152, and 162 were extracted using the two-potential approach. The nuclei around the shell closure were more tightly bound than adjacent nuclei. Additionally, based on the WS4 mass model (Wang et al., Phys. Lett.B 734, 215 (2014)), we extended the two-potential approach to predict the α-decay half-lives of nuclei around N values of178 and 184 with Z of 119 and 120. We believe that our findings will serve as guidelines for future experimental studies.展开更多
During the automated placement process of dry fibers,the positioning and fixation of dry fiber gauze belts are achieved by spraying setting agents.The amount of the setting agent is difficult to control when it is spr...During the automated placement process of dry fibers,the positioning and fixation of dry fiber gauze belts are achieved by spraying setting agents.The amount of the setting agent is difficult to control when it is sprayed manually.Furthermore,it can also affect the permeability of the preform,resin injection and the quality of the vacuum assisted resin infusion(VARI)molding,resulting in a decrease in the mechanical properties of composite materials.This study utilizes dry fiber automated placement equipment and an automated spraying system to manufacture preform structures,followed by VARI process to prepare composite samples with varying setting agent contents.Subsequently,mechanical characterization including interlaminar shear,bending and tensile testing is conducted to investigate the influence of setting agent content on both the manufacturing process and the mechanical properties of composite products.The results show that the interlaminar shear strength,bending strength and tensile strength of the sample gradually decrease with the increase of the content of the setting agent.The optimal setting agent content for automated laying of dry fiber is determined to be 4%-6%,balancing the preformed body’s layup quality and its impact on mechanical properties.Compared with agent-free samples,this range results in reductions of 3% in interlaminar shear strength,9% in bending strength,11% in bending modulus,and 13%-16% in tensile strength.展开更多
The final quality of complex conical-section rings depends on co-design of multiple processes in forming process chain.In this study,for a complex aeroengine casing ring with a large slope and a flange on its end,a co...The final quality of complex conical-section rings depends on co-design of multiple processes in forming process chain.In this study,for a complex aeroengine casing ring with a large slope and a flange on its end,a co-design method of the forming process chain is put forward towards the objective of precision forming,which not only proposes a standard process route composed of multiple processes of upsetting,punching,rectangular ring rolling,loose tooling forging and profiled ring rolling,but also presents co-design methods of dies and blanks for all the processes.For profiled ring rolling,a design method of preformed blank that makes the blank and the target conical-section ring have the same axial volume distribution is proposed.By the method,the axial metal redistribution during the process can be alleviated greatly thus improving the forming stability and precision of the ring.Based on the geometric features of designed preformed blank,design methods of blanks and dies for loose tolling forging,rectangular ring rolling,punching and upsetting are proposed sequentially.In view of the key roles of loose tooling forging(manufacturing the preformed blank)and profiled ring rolling on the final quality of the conical ring parts,inherited FE simulations for these two processes are performed to verify the proposed design methods and determine appropriate design parameter.It is demonstrated that the proposed design method has significant advantages in improving forming precision.Besides,a suggestive value 1.5 of the rolling ratio for profiled ring rolling(a key design parameter)is given based on comprehensive consideration of multiple indicators such as ring roundness,deformation uniformity and forming load.The corresponding industrial experiments performed illustrate that a high forming precision of the conical-section aeroengine casing ring is achieved.展开更多
Variable-diameter deployable carbon fiber reinforced polymer(CFRP)composites possess deformation and load-bearing functions and are composed of stiff-flexible coupled preforms and matrix.The stiff-flexible coupled pre...Variable-diameter deployable carbon fiber reinforced polymer(CFRP)composites possess deformation and load-bearing functions and are composed of stiff-flexible coupled preforms and matrix.The stiff-flexible coupled preform,serving as the reinforcing structure,directly determines the deployable properties,and its forming technology is currently a research challenge.This paper designs a braiding and needle-punching(BNP)composite preform forming technology suitable for stiff-flexible coupled preforms.Before forming,the preform is partitioned into flexible and rigid zones,with braiding and needle-punching performed layer by layer in the respective zones.A retractable rotating device is developed to form the stiff-flexible coupled preform,achieving a diameter variation rate of up to 26.6%for the BNP preform.A structural parameter model is also established to describe the geometric parameter changes in the deformation and load-bearing areas of the preform during deployment as a function of the braiding angle.Based on experiments,this paper explains the performance changes of BNP composites concerning the structural parameters of the preform.Experimental analysis shows that as the braiding angle increases,the tensile performance of BNP composites significantly decreases,with the change rate of tensile strength first decreasing and then increasing.Additionally,when the braiding angle is less than 21.89°,the impact toughness of BNP composites remains within the range of 83.66±2 kJ/m^(2).However,when the braiding angle exceeds 21.89°,the impact toughness of BNP composites gradually decreases with increasing braiding angle.Furthermore,a hybrid agent model based on Latin hypercube sampling and error back-propagation neural network is developed to predict the tensile and impact properties of BNP composites with different structural parameters,with maximum test relative errors of 1.89%for tensile strength and 2.37%for impact toughness.展开更多
This article, in order to improve the deformation homogeneity in aerospace forgings, proposes an approach that combines the fnite element method （FEM） and the response surface method （RSM） to optimize the preform ...This article, in order to improve the deformation homogeneity in aerospace forgings, proposes an approach that combines the fnite element method （FEM） and the response surface method （RSM） to optimize the preform shapes. New expressions that take into account the influences of equivalent effective strain distribution are developed to evaluate the homogeneity of deformation distribution in aerospace forgings. In order to reduce the number of design variables, the domain-division method is put forward to determine the optimal design variables. On the basis of FEM results, the RSM is used to establish an approximate model to depict the relationship between the responses （deformation homogeneity and die underfilling） and the design variables repre- sented by geometric parameters of the preform shape. With a typical aeroengine disk as an example, the proposed method is verified by achieving an optimal combination of design variables. By comparing the preform shape obtained with the proposed method to that with the existing one, it is evidenced that the former could achieve more homogeneous deformation in forging.展开更多
C/C composites with banded structure pyrocarbon were fabricated by fast chemical vapor infiltration（CVI）,with C3H6 as carbon source,N2 as carrier gas,and three-dimensional（3D） 12K PAN-based carbon fabric with high...C/C composites with banded structure pyrocarbon were fabricated by fast chemical vapor infiltration（CVI）,with C3H6 as carbon source,N2 as carrier gas,and three-dimensional（3D） 12K PAN-based carbon fabric with high density of 0.94 g/cm3 as preform.Experimental results indicated that the fracture characteristics of C/C composites were closely related to the frequency of high-temperature treatment（HTT） at the break of CVI process.According to the load？displacement curves,C/C composites showed a pseudoplastic fracture after twice of HTT.After three times of HTT,load？displacement curves tended to be stable with a decreasing bending strength at 177.5 MPa.Delamination failure and intrastratal fiber fracture were observed at the cross-section of C/C composites by scanning electronic microscope.Because the content of pyrocarbon and fibers has a different distribution in layers,the C/C composites show different fracture characteristics at various regions,which leads to good toughness and bending strength.展开更多
The cast preformed forming process(CPFP) is increasingly considered and applied in the metal forming industries due to its short process, low cost, and environmental friendliness, especially in the aerospace field. Ho...The cast preformed forming process(CPFP) is increasingly considered and applied in the metal forming industries due to its short process, low cost, and environmental friendliness, especially in the aerospace field. However, how to establish a unified model of a non-uniform as-cast billet depicting the flow stress and microstructure evolution behaviors during hot working is the key to microstructure prediction and parameter optimization of the CPFP. In this work, hot compression tests are performed using a non-uniform as-cast 42 CrMo billet at 1123–1423 K and 0.01–1sà1. The effect laws of the non-uniform state of the as-cast billet with different initial grain sizes on the flow stress and microstructure are revealed deeply. Based on experimental results, a unified model of flow stress and grain size evolutions is developed by the internal variable modeling method. Verified results show that the model can well describe the responses of the flow stress and microstructure to deformation conditions and initial grain sizes. To further evaluate its reliability, the unified model is applied to FE simulation of the cast preformed ring rolling process.The predictions of the rolling force and grain size indicate that it could well describe the flow stress and microstructure evolutions during the process.展开更多
On the basis of the minimum energy principle and the minimum resistance law,this article proposes a new method,termed equipotential field method,to design the proper preform for producing isothermo forged P/M superall...On the basis of the minimum energy principle and the minimum resistance law,this article proposes a new method,termed equipotential field method,to design the proper preform for producing isothermo forged P/M superalloy disks. Using this new method,six variant preform contours are acquired with software ANSYS. The isothermal forging process of the P/M superalloy disk is simulated by using the industrial software MSC/Superform with thus obtained preforms so as to achieve the equivalent strain distribution in...展开更多
In this paper, microscopic characteristics of preformed gels （PGs） and secondary cross-linked gels （SCG） with the same concentration were analyzed by atomic force microscopy （AFM）. Experimental results indicate ...In this paper, microscopic characteristics of preformed gels （PGs） and secondary cross-linked gels （SCG） with the same concentration were analyzed by atomic force microscopy （AFM）. Experimental results indicate that the microstructure of secondary cross-linked gels is a thick 3-D network, in which micro-holes and irregular macro-holes are embedded. The maximum width of the irregular macro-holes is 200 nm. In the SCG two different chemical bonds were formed, which leads to the structural inhomogeneity and the asymmetry of the crosslinking density. The structural inhomogeneity of SCG results in the formation of irregular macro-holes. The excessive cross-linking density is the primary reason for dehydration of SCG and the presence of irregular macro-holes in SCG can facilitate dehydration.展开更多
基金financially supported by the National Basic Research Program of China(973 Program,No.2015CB932100)National Natural Science Foundation of China(No.81703451,81573371,81473164)。
文摘Preformed albumin corona of albumin-nonselective nanoparticles(NPs)is widely exploited to inhibit the unavoidable protein adsorption upon intravenous administration.However,very few studies have concerned the preformed albumin corona of albumin-selective NPs.Herein,we report a novel type of albumin-selective NPs by decorating 6-maleimidocaproyl polyethylene glycol stearate(SA)onto PLGA NPs(SP NPs)surface,taking albuminnonselective PLGA NPs as control.PLGA NPs and SP NPs were prepared by emulsion-solvent evaporation method and the resultant NPs were in spherical shape with an average diameter around 180 nm.The corresponding albumin-coating PLGA NPs(PLGA@BSA NPs)and albumin-coating SP NPs(SP@BSA NPs)were formulated by incubating SP NPs or PLGA NPs with bovine serum albumin solution,respectively.The impact of albumin corona on particle characteristics,stability,photothermal effect,cytotoxicity,cell uptake,spheroid penetration and pharmacokinetics was investigated.In line with previous findings of preformed albumin coating,PLGA@BSA NPs exhibited higher stability,cytotoxicity,cell internalization and spheroid penetration performances in vitro,and longer blood circulation time in vivo than those of albumin-nonselective PLGA NPs,but albumin-selective SP NPs is capable of achieving a comparable in vitro and in vivo performances with both SP@BSA NPs and PLGA@BSA NPs.Our results demonstrate that SA decorated albumin-selective NPs pave a versatile avenue for optimizing nanoparticulate delivery without preformed albumin corona.
基金supported by the Research Foundation of China University of Petroleum-Beijing at Karamay (No. YJ2018B02002 and XQZX20200010)the Natural Science Foundation of Xinjiang Uygur Autonomous Region (No. 2021D01E23 and 2019D01B57)+3 种基金the University Scientific Research Project of Xinjiang Uygur Autonomous Region (No. XJEDU2019Y067)the Xinjiang Uygur Autonomous Region Innovation Environment Construction Project (No. 2019Q025)the Sichuan Province Regional Innovation Cooperation Project (No. 2020YFQ0036)the CNPC Strategic Cooperation Science and Technology Project (ZLZX2020-01-04-04)
文摘The development of unconventional petroleum resources has gradually become an important succession for increasing oil production.However,the related engineers and researchers are paying more and more attention to the application of temporary plugging agents(TPAs)for their efficient development.TPAs can expand the stimulated reservoir volume(SRV)and facilitate the flow of oil and gas to the bottom of the well.Particle-gels used as temporary plugging agents have the characteristics of the simple injection process,good deformation,high plugging strength,and complete self-degradation performance,which have been widely applied in recent years.In this paper,five samples of DPPG polymerized by different molecular weights of cross-linking agents were prepared.In addition,infrared spectroscopy analysis,differential calorimetry scanning(DSC)analysis,static particle gel swelling and degradation performance evaluation experiments,and dynamic temporary plugging performance experiments in cores were conducted at 34°C.Results show that as the molecular weight of the cross-linking agent(at 0.01 g)in the DPPG molecule decreased from 1,000 to 200 Da,the fewer cross-linking sites of DPPG,the looser the microscopic three-dimensional mesh structure formed.The swelling ratio increased from 7 to 33 times.However,the complete degradation time increased from 40 to 210 min.Moreover,the DSC results confirmed that the higher the molecular weight of the cross-linking agent,the worse is chemical stability and the more prone it to self-degradation.DPPG samples had good temporary plugging performance in reservoir cores.DPPGs prepared by the cross-linking agent with smaller molecular weight has a stronger swelling ratio,higher gel strength,and greater plugging strength in the core permeabilities.Moreover,the degraded DPPG is less damaging to the cores.However,their slower degradation rates take a slightly longer times to reach complete degradation.The results of this paper can provide new ideas and a theoretical basis for the development of particle gel-type temporary plugging agents(TPA)with controllable degradation time in low-temperature reservoirs.It can help to expand the application range of existing DPPG reservoir conditions.
文摘The paper discusses the difference in the formulation concepts of alumina - spinel （spinel containing ） and alumina - magnesia （ spin, el forming） castables and the influence on their physical properties. The individual property profile is discussed with respect to the requirements of refactory lining materials for the different zones of a steel ladle.
文摘Model of hole-pairs in electrical transport along ab plane in cuprate superconductors has already been proposed. It has been found to be in the shape of 3dx2–y2 orbital of an electron in an atom. This time, model of hole-pairs in transport along c-axis in cuprate superconductors is proposed. In ab-plane, hole-pairs are formed along CuO2 plane;one hole-pair covering 9 - 10 two dimensional CuO2 unit cells in 3dx2–y2 configuration. In the investigation of c-axis hole-pairs, cuprate superconductors have been sub-divided into three categories depending on the number of CuO2 planes/formula unit. There is a little different treatment for finding out the order parameter in each category. Coherence lengths along ab-planes are of the order of a few tens of Angstroms, whereas along c-axis, they are less than even their a-, b-lattice constants. In cuprates with 2 or 3 CuO2 planes, the order parameter is of 3dz2–x2 type in zx-plane with lobes along both the axes much constrained. For cuprates with a single CuO2 layer, the order parameter is of 3dx2–y2 type, but its dimensions are less than a-, b-lattice constants.
基金a grantfrom the National Natural Science Foundation of China(No.39370 70 2 )
文摘The reactivity of human preformed natural antibodies (PNAbs) with various porcine pancreatic cells and its isotypes was investigated. Eighteen serum samples from patients with insulin dependent diabetes mellitus (IDDM) and 20 serum samples from healthy human subjects were collected. The frozen sections of the pig pancreas were incubated with these sera, and subsequently incubated with FITC conjugated goat antihuman IgG and IgM monoclonal antibodies. The reactivity of human PNAbs with various porcine pancreatic cells was determined by indirect immunofluorescence staining technique. The results showed that 55.6 % of IDDM patients and 55.0 % of healthy human individuals contained PNAbs against porcine endocrine cells. However, the percentage of strongly reacting sera in the patient group was significantly increased as compared with that in the control group. All used sera from IDDM patients and 95 % of sera from healthy controls could react to one or more of the various pancreatic cell types, including: endocrine cells, exocrine cells, vascular endothelial cells, ductal epithelial cells and macrophages. The isotypes of PNAbs contained both IgG and IgM. In view of strongly positive reactivity of PNAbs with various porcine pancreatic cells, pretransplantly cross matching test and graft pretreatment may be necessary for survival of islet transplants.
文摘A model of preformed hole-pairs in cuprate superconductors has been proposed based on some experimental results i.e., 1) electron paramagnetic resonance spectra of quenched superconductors which show very frequently the fragment (CuO)4 broken off from the CuO2 layer in the structure, 2) 41 meV peak observed in neutron diffraction and nuclear magnetic resonance spectra of superconductors, 3) Heisenberg exchange interaction leading to ferromagnetism observed in CuO which is an essential ingredient of all superconductors and some generally accepted conclusions i.e., a) that the order parameter in superconductors has dx2–y2 symmetr and b) coherence length is of the order of 15 - 20 Angstrom. Heisenberg exchange interaction between two (CuO4) plaquettes each containing a lattice hole binds the two holes which are the charge carriers in the cuprate superconductors. It is not very clear whether the hole-pair is in the triplet or singlet state, but the triplet state is supported by the experimental observation of ferromagnetism in the parent material CuO. The proposed hole-pair singlet is different from Zhang-Rice singlet.
基金Supported by the U.S.Department of Energy’s Office of Energy Efficiency and Renewable Energy(EERE)under the Geothermal Technologies Office(GTO)“Innovative Methods to Control Hydraulic Properties of Enhanced Geothermal Systems”(DE-EE0009790).
文摘To understand the applicability of high-temperature preformed particle gel(HT-PPG)for control of short-circuiting in enhanced geothermal systems(EGSs),core flooding experiments were conducted on fractured granite cores under varying fracture widths,gel particle sizes and swelling ratios.Key parameters such as injection pressure,water breakthrough pressure,and residual resistance factor were measured to evaluate HT-PPG performance.The gel exhibited strong injectability,entering granite fractures at pressure gradients as low as 0.656 MPa/m;HT-PPG yields a superior sealing performance by significantly reducing the permeability;and dehydration occurs during HT-PPG propagation,with a dehydration ratio ranging from 4.71%to 11.36%.This study reveals that HT-PPG can be injected into geothermal formations with minimal pressure yet provides strong resistance to breakthrough once in place.This balance of injectability and sealing strength makes HT-PPG effective for addressing thermal short-circuiting in EGS reservoirs.
文摘Re-crosslinkable preformed particle gel(RPPG)has been considered to be one of the most promising gels for dealing with fracture and void space conduit(VSC)conformance problems.However,the dehydration of RPPG during its propagation in the fracture-type features and its effect on gel properties remains unclear.This paper investigates the dehydration behavior during RPPG propagating in an open fracture using matrix-free fracture model.Then the results were verified using real fractured sandstone core model.Moreover,the gel properties after extruding a fracture were studied in detail including gel dehydration and gel strength.Results reveal that the RPPG properties changed significantly with increasing propagation distance,which correlated with the gel injection rate.At high gel injection rates,the dehydration and gel strength(Gʹ)decrease with increasing propagation distance.In contrast,the opposite result was found at low injection rates.Based on the study of the different gel injection rates,it is found that dehydration time is another key factor affecting dehydration behavior.Results also indicate that the fracture width affects gel dehydration at different locations.Dehydration was more pronounced at narrow fractures but only in the inlet section,while in the outlet section,RPPG contains more water than the initial condition.This study has profound implications for field applications.It provides new insights into the transport of RPPG in fractures and helps field engineers to optimize the gel injection operations.
基金supported in part by the National Nature Science Foundation of China(81830064,81721092,32000969,82002056)Key Support Program for Growth Factor Research(SZYZ-TR-03)+3 种基金Chinese PLA General Hospital for Military Medical Innovation Research Project(CX-19026)the CAMS Innovation Fund for Medical Sciences(CIFMS,2019-I2M-5-059)the Military Medical Research and Development Projects(AWS17J005)National Key Research and Development Program of China(2018YFA0108700,2017YFA0105602).
文摘The therapeutic interventions of human hypertrophic scars(HHS)remain puzzle largely due to the lack of accepted models.Current HHS models are limited by their inability to mimic native scar architecture and associated pathological microenvironments.Here,we create a 3D functional HHS model by preformed cellular aggregates(PCA)bioprinting,firstly developing bioink from scar decellularized extracellular matrix(ECM)and alginate-gelatin(Alg-Gel)hydrogel with suitable physical properties to mimic the microenvironmental factors,then pre-culturing patient-derived fibroblasts in this bioink to preform the topographic cellular aggregates for sequent printing.We confirm the cell aggregates preformed in bioink displayed well defined aligned structure and formed functional scar tissue self-organization after bioprinting,hence showing the potential of creating HHS models.Notably,these HHS models exhibit characteristics of early-stage HHS in gene and protein expression,which significantly activated signaling pathway related to inflammation and cell proliferation,and recapitulate in vivo tissue dynamics of scar forming.We also use the in vitro and in vivo models to define the clinically observed effects to treatment with concurrent anti-scarring drugs,and the data show that it can be used to evaluate the potential therapeutic target for drug testing.The ideal humanized scar models we present should prove useful for studying critical mechanisms underlying HHS and to rapidly test new drug targets and develop patient-specific optimal therapeutic strategies in the future.
基金the National Natural Science Foundation of China (Nos. 29825108, 20173022 and 20121103), the Major State Basic Research Development Program (No. 20000077507) and the National Advanced Materials Committee of China.
文摘The acidic strength of ordered mesoporous aluminosilicates of MAS-2, MAS-3, MAS-7 and MAS-9 and mi-croporous crystals of Y, L, beta, and ZSM-5 zeolites was systemically investigated by temperature-programmed desorption of ammonia (NH3-TPD). Due to the use of preformed zeolite precursors of Y, L, beta and ZSM-5, the ordered mesoporous aluminosilicates with distinguished acidic strength were obtained, being dependent on the type of preformed zeolite precursors. Therefore, the acidic strength of these mesoporous aluminosilicates could be tai-lored and controlled.
基金The work was supported by the National Natural Science Foundation of China(No.51674208)Sichuan Provincial Key Labo-ratory of Applied Chemistry for Oil and Gas Field Open Fund(YQKF202010)Southwest Petroleum University College Students Open Experimental Fund(2020KSZ33001,2020KSZ04047).
文摘Inspired by the viscoelastic displacement theory,a product called preformed particle gel(PPG)is developed as conformance control agent to enhance oil recovery and control excess water production.The migration law of PPG suspension in porous media is related to its deep profile control and displacement capability.Laboratory experiments indicate that PPG suspension has good viscosity increasing,and the apparent viscosity decreases with the increase of shear rate.PPG suspension is mainly elastic,and its network structure makes it have certain shear stability.PPG particles realize migration in porous media in the way of“accumulation and blockage/pressure increase/deformation and migration”.When the ratio of the PPG particle size to the pore throat diameter d ranges from 35.52 to 53.38,the particles can match through the porous medium.When the permeability difference of the parallel model is 5,PPG suspension has the highest profile improvement rate,69.10%.PPG suspension can adjust the planar heterogeneity,and increase the oil recovery rate by 20.75%.The PPG suspension can effectively start“cluster"、“film”and“blind end residual oil”,and has a high oil washing efficiency.The core NMR T2 spectrum shows that PPG suspension mainly reduces oil saturation in mesopores and macropores.After PPG flooding,the EOR capacity of small pores is the highest,39.11%.
基金supported in part by the National Natural Science Foundation of China(Nos.12175100 and 11975132)Construct Program of the Key Discipline in Hunan Province,Research Foundation of Education Bureau of Hunan Province,China(Nos.21B0402,18A237 and 22A0305)+3 种基金Natural Science Foundation of Hunan Province,China(No.2018JJ2321)Innovation Group of Nuclear and Particle Physics in USC,Shandong Province Natural Science Foundation,China(No.ZR2022JQ04)Opening Project of Cooperative Innovation Center for Nuclear Fuel Cycle Technology and Equipment,University of South China(No.2019KFZ10)Hunan Provincial Innovation Foundation for Postgraduate(No.CX20230962).
文摘By combining experimental α-decay energies and half-lives, the α-particle preformation factor for nuclei around neutron magic numbers N of 126, 152, and 162 were extracted using the two-potential approach. The nuclei around the shell closure were more tightly bound than adjacent nuclei. Additionally, based on the WS4 mass model (Wang et al., Phys. Lett.B 734, 215 (2014)), we extended the two-potential approach to predict the α-decay half-lives of nuclei around N values of178 and 184 with Z of 119 and 120. We believe that our findings will serve as guidelines for future experimental studies.
基金supported by Jiangsu Provincial Key Research and Development Program(No.BE2023014-4).
文摘During the automated placement process of dry fibers,the positioning and fixation of dry fiber gauze belts are achieved by spraying setting agents.The amount of the setting agent is difficult to control when it is sprayed manually.Furthermore,it can also affect the permeability of the preform,resin injection and the quality of the vacuum assisted resin infusion(VARI)molding,resulting in a decrease in the mechanical properties of composite materials.This study utilizes dry fiber automated placement equipment and an automated spraying system to manufacture preform structures,followed by VARI process to prepare composite samples with varying setting agent contents.Subsequently,mechanical characterization including interlaminar shear,bending and tensile testing is conducted to investigate the influence of setting agent content on both the manufacturing process and the mechanical properties of composite products.The results show that the interlaminar shear strength,bending strength and tensile strength of the sample gradually decrease with the increase of the content of the setting agent.The optimal setting agent content for automated laying of dry fiber is determined to be 4%-6%,balancing the preformed body’s layup quality and its impact on mechanical properties.Compared with agent-free samples,this range results in reductions of 3% in interlaminar shear strength,9% in bending strength,11% in bending modulus,and 13%-16% in tensile strength.
基金the National Natural Science Foundation of China(52275378).
文摘The final quality of complex conical-section rings depends on co-design of multiple processes in forming process chain.In this study,for a complex aeroengine casing ring with a large slope and a flange on its end,a co-design method of the forming process chain is put forward towards the objective of precision forming,which not only proposes a standard process route composed of multiple processes of upsetting,punching,rectangular ring rolling,loose tooling forging and profiled ring rolling,but also presents co-design methods of dies and blanks for all the processes.For profiled ring rolling,a design method of preformed blank that makes the blank and the target conical-section ring have the same axial volume distribution is proposed.By the method,the axial metal redistribution during the process can be alleviated greatly thus improving the forming stability and precision of the ring.Based on the geometric features of designed preformed blank,design methods of blanks and dies for loose tolling forging,rectangular ring rolling,punching and upsetting are proposed sequentially.In view of the key roles of loose tooling forging(manufacturing the preformed blank)and profiled ring rolling on the final quality of the conical ring parts,inherited FE simulations for these two processes are performed to verify the proposed design methods and determine appropriate design parameter.It is demonstrated that the proposed design method has significant advantages in improving forming precision.Besides,a suggestive value 1.5 of the rolling ratio for profiled ring rolling(a key design parameter)is given based on comprehensive consideration of multiple indicators such as ring roundness,deformation uniformity and forming load.The corresponding industrial experiments performed illustrate that a high forming precision of the conical-section aeroengine casing ring is achieved.
基金Supported by Jiangsu Provincial Frontier Leading Technology Basic Research Project(Grant No.BK20212007)Aero-Engine and Gas Turbine Basic Science Center(Grant No.P2022-B-IV-014-001)+1 种基金China Postdoctoral Program Fund(Grant No.1005/YBA23044)China Postdoctoral Assistance Fund(Grant No.1005/YBA23031)。
文摘Variable-diameter deployable carbon fiber reinforced polymer(CFRP)composites possess deformation and load-bearing functions and are composed of stiff-flexible coupled preforms and matrix.The stiff-flexible coupled preform,serving as the reinforcing structure,directly determines the deployable properties,and its forming technology is currently a research challenge.This paper designs a braiding and needle-punching(BNP)composite preform forming technology suitable for stiff-flexible coupled preforms.Before forming,the preform is partitioned into flexible and rigid zones,with braiding and needle-punching performed layer by layer in the respective zones.A retractable rotating device is developed to form the stiff-flexible coupled preform,achieving a diameter variation rate of up to 26.6%for the BNP preform.A structural parameter model is also established to describe the geometric parameter changes in the deformation and load-bearing areas of the preform during deployment as a function of the braiding angle.Based on experiments,this paper explains the performance changes of BNP composites concerning the structural parameters of the preform.Experimental analysis shows that as the braiding angle increases,the tensile performance of BNP composites significantly decreases,with the change rate of tensile strength first decreasing and then increasing.Additionally,when the braiding angle is less than 21.89°,the impact toughness of BNP composites remains within the range of 83.66±2 kJ/m^(2).However,when the braiding angle exceeds 21.89°,the impact toughness of BNP composites gradually decreases with increasing braiding angle.Furthermore,a hybrid agent model based on Latin hypercube sampling and error back-propagation neural network is developed to predict the tensile and impact properties of BNP composites with different structural parameters,with maximum test relative errors of 1.89%for tensile strength and 2.37%for impact toughness.
文摘This article, in order to improve the deformation homogeneity in aerospace forgings, proposes an approach that combines the fnite element method （FEM） and the response surface method （RSM） to optimize the preform shapes. New expressions that take into account the influences of equivalent effective strain distribution are developed to evaluate the homogeneity of deformation distribution in aerospace forgings. In order to reduce the number of design variables, the domain-division method is put forward to determine the optimal design variables. On the basis of FEM results, the RSM is used to establish an approximate model to depict the relationship between the responses （deformation homogeneity and die underfilling） and the design variables repre- sented by geometric parameters of the preform shape. With a typical aeroengine disk as an example, the proposed method is verified by achieving an optimal combination of design variables. By comparing the preform shape obtained with the proposed method to that with the existing one, it is evidenced that the former could achieve more homogeneous deformation in forging.
基金Project (50802115) supported by the National Natural Science Foundation of ChinaProject (2011CB605801) supported by the National Basic Research Program of China
文摘C/C composites with banded structure pyrocarbon were fabricated by fast chemical vapor infiltration（CVI）,with C3H6 as carbon source,N2 as carrier gas,and three-dimensional（3D） 12K PAN-based carbon fabric with high density of 0.94 g/cm3 as preform.Experimental results indicated that the fracture characteristics of C/C composites were closely related to the frequency of high-temperature treatment（HTT） at the break of CVI process.According to the load？displacement curves,C/C composites showed a pseudoplastic fracture after twice of HTT.After three times of HTT,load？displacement curves tended to be stable with a decreasing bending strength at 177.5 MPa.Delamination failure and intrastratal fiber fracture were observed at the cross-section of C/C composites by scanning electronic microscope.Because the content of pyrocarbon and fibers has a different distribution in layers,the C/C composites show different fracture characteristics at various regions,which leads to good toughness and bending strength.
基金supported by the National Natural Science Foundation of China (No’s. 51575448 and 51135007)
文摘The cast preformed forming process(CPFP) is increasingly considered and applied in the metal forming industries due to its short process, low cost, and environmental friendliness, especially in the aerospace field. However, how to establish a unified model of a non-uniform as-cast billet depicting the flow stress and microstructure evolution behaviors during hot working is the key to microstructure prediction and parameter optimization of the CPFP. In this work, hot compression tests are performed using a non-uniform as-cast 42 CrMo billet at 1123–1423 K and 0.01–1sà1. The effect laws of the non-uniform state of the as-cast billet with different initial grain sizes on the flow stress and microstructure are revealed deeply. Based on experimental results, a unified model of flow stress and grain size evolutions is developed by the internal variable modeling method. Verified results show that the model can well describe the responses of the flow stress and microstructure to deformation conditions and initial grain sizes. To further evaluate its reliability, the unified model is applied to FE simulation of the cast preformed ring rolling process.The predictions of the rolling force and grain size indicate that it could well describe the flow stress and microstructure evolutions during the process.
基金Aeronautical Science Foundation of China (03H53048)
文摘On the basis of the minimum energy principle and the minimum resistance law,this article proposes a new method,termed equipotential field method,to design the proper preform for producing isothermo forged P/M superalloy disks. Using this new method,six variant preform contours are acquired with software ANSYS. The isothermal forging process of the P/M superalloy disk is simulated by using the industrial software MSC/Superform with thus obtained preforms so as to achieve the equivalent strain distribution in...
文摘In this paper, microscopic characteristics of preformed gels （PGs） and secondary cross-linked gels （SCG） with the same concentration were analyzed by atomic force microscopy （AFM）. Experimental results indicate that the microstructure of secondary cross-linked gels is a thick 3-D network, in which micro-holes and irregular macro-holes are embedded. The maximum width of the irregular macro-holes is 200 nm. In the SCG two different chemical bonds were formed, which leads to the structural inhomogeneity and the asymmetry of the crosslinking density. The structural inhomogeneity of SCG results in the formation of irregular macro-holes. The excessive cross-linking density is the primary reason for dehydration of SCG and the presence of irregular macro-holes in SCG can facilitate dehydration.
