This paper highlights the innovative approach and findings of the recently published study by Xu et al,which underscores the integration of radiomics and clinicoradiological factors to enhance the preoperative predict...This paper highlights the innovative approach and findings of the recently published study by Xu et al,which underscores the integration of radiomics and clinicoradiological factors to enhance the preoperative prediction of microvascular invasion in patients with hepatitis B virus-related hepatocellular carcinoma(HBV-HCC).The study’s use of contrast-enhanced computed tomography radiomics to construct predictive models offers a significant advancement in the surgical planning and management of HBV-HCC,potentially transforming patient outcomes through more personalized treatment strategies.This editorial commends the study's contribution to precision medicine and discusses its implic-ations for future research and clinical practice.展开更多
Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging sene...Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associatedβ-galactosidase(SA-β-Gal),which is proved to be closely associated with senescent cells,the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associatedβ-Gal(OA-β-Gal)will interfere with imaging accuracy.Herein,a near-infrared(NIR)hemicyanine based fluorescent probe HCyXA-βGal was designed for light-up imaging of live cells containingβ-Gal.With the organelle-targeting morpholinyl and positive charge moieties,HCyxA-βGal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells.Furthermore,inspired by the fast response performance,fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal:time-dependent intensity.展开更多
In situ precise detection of bioactive molecules with high sensitivity and spatiotemporal resolution is essential for studying physiological events and disease diagnosis.The utilization of versatile fluorescent probes...In situ precise detection of bioactive molecules with high sensitivity and spatiotemporal resolution is essential for studying physiological events and disease diagnosis.The utilization of versatile fluorescent probes in fluorescence imaging offers a powerful tool for in vivo imaging of biomarkers closely associated with pathological conditions.However,the dynamic behavior leading to rapid clearance of small molecule probes from regions of interest severely compromises their potential for precise imaging.Notably,self-immobilizing fluorescent probes that selectively recognize diseased tissues while improving in situ retention and enrichment enable accurate high-fidelity fluorescence imaging.In this review,we aim to summarize the strategies employed for recent advances in the performance and precision of in vivo fluorescence imaging using self-immobilizing techniques.Lastly,we discuss the prospects and potential challenges associated with selfimmobilizing fluorescent probes to promote further development and application of more delicate fluorescent probes.展开更多
Atomic force microscope(AFM)systems rely on silicon(Si)probes for precise nanoscale characterization across diverse environments.However,fabricating high-aspect-ratio(HAR)and sharp Si tips and optimizing the handle ge...Atomic force microscope(AFM)systems rely on silicon(Si)probes for precise nanoscale characterization across diverse environments.However,fabricating high-aspect-ratio(HAR)and sharp Si tips and optimizing the handle geometries remain significant challenges.Conventional HAR probe fabrication methods lack scalability,precision,and cost efficiency,while cuboid-shaped handles risk obstructing laser detection and limiting compatibility.This study presents an innovative batch-fabrication strategy for high-performance Si AFM probes that integrate ultra-sharp HAR tips,rectangular cantilevers,and universally compatible stair-shaped handles.Notably,the tip fabrication process employs only low-cost microscale ultraviolet(UV)lithography,while still achieving nanoscale structural resolution.The fabricated probes exhibit a tip apex radius of 5 nm and a half-cone angle of 7.5°,enabling high-resolution and highfidelity imaging.The novel stair-shaped handle geometry is introduced and fabricated via a single-step dry etching process,which provides unobstructed laser detection and ensures compatibility with a broad range of commercial AFM platforms.Durability testing demonstrates stable scanning performance for up to 8 hours within the 100 nm precision range,confirming the mechanical reliability of the design.This scalable,reproducible,and high-yield fabrication strategy represents a significant advancement in HAR AFM probe development,providing enhanced performance and extended applicability for diverse nanoscale imaging applications.展开更多
Molecular imaging has emerged as a transformative tool in cancer diagnosis,enabling the visualization of biological processes at the cellular and molecular levels.Aptamers,single-stranded oligonucleotides with high af...Molecular imaging has emerged as a transformative tool in cancer diagnosis,enabling the visualization of biological processes at the cellular and molecular levels.Aptamers,single-stranded oligonucleotides with high affinity and specificity for target molecules,have gained significant attention as versatile probes for molecular imaging due to their unique properties,including small size,ease of modification,low immunogenicity,and rapid tissue penetration.This review explores the integration of aptamers with various imaging agents to enhance cancer diagnosis and therapy.Aptamer-based imaging probes offer high sensitivity and real-time visualization of tumor markers.Aptamer-based fluorescence probes and aptamer-conjugated magnetic resonance imaging(MRI)probes,including gadolinium-based contrast agents,improve tumor targeting and imaging resolution.Additionally,aptamers have been utilized in single-photon emission computed tomography(SPECT)and positron emission tomography(PET)imaging to enhance the specificity of radiotracers for cancer detection.Furthermore,aptamer-targeted ultrasound and computed tomography(CT)imaging demonstrate the potential for noninvasive and precise tumor localization.By leveraging the unique advantages of aptamers,these imaging strategies not only improve diagnostic accuracy but also pave the way for image-guided cancer therapies.This review highlights the significant role of aptamers in advancing molecular imaging and their potential to revolutionize cancer diagnosis and treatment.展开更多
Nanobodies,single-domain antibodies derived from camelid heavy-chain antibodies,have emerged as powerful molecular tools in cancer diagnosis and therapy due to their unique structural and functional attributes,includi...Nanobodies,single-domain antibodies derived from camelid heavy-chain antibodies,have emerged as powerful molecular tools in cancer diagnosis and therapy due to their unique structural and functional attributes,including small size,high antigen specificity,robust stability,and ease of genetic and chemical modification.This Review provides an extensive overview of the nanobody structure,characteristics,and production methods,highlighting their advantages over conventional antibodies.We comprehensively discuss various applications of nanobodies in cancer diagnosis and therapy,encompassing their roles as antagonists or agonists in modulating oncogenic and immune-related signaling pathways,targeted delivery systems for chemotherapeutics,radionuclides,immunotoxins,and photo-sensitizers,and their integration into advanced imaging technologies such as PET,SPECT,and optical imaging for accurate tumor detection and monitoring.Additionally,we explore the development of innovative cell-based immunotherapies leveraging nanobody-engineered chimeric antigen receptor(CAR)-modified T cells,natural killer cells,and macrophages,illustrating their potential to enhance therapeutic precision and efficacy.Finally,we discuss the current challenges and provide perspectives on emerging nanobody technologies and their translational prospects,underscoring the potential of nanobody-based toolkits to drive next-generation precision cancer diagnostics and therapies.展开更多
This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth obser...This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth observation program. In addition, this paper expounded the transformation mechanism and procedure from earth observation data to geospatial information and geographical knowledge, and examined the key scientific and technological issues, including earth observation networks, high-precision image positioning, image understanding, automatic spatial information extraction, and focus services. These analyses provide a new impetus for pushing the application of China's high-resolution earth observation system from a "quantity" to "quality" change, from China to the world, from providing products to providing online service.展开更多
基金Supported by Shandong Province Medical and Health Science and Technology Development Plan Project,No.202203030713Science and Technology Program of Yantai Affiliated Hospital of Binzhou Medical University,No.YTFY2022KYQD06。
文摘This paper highlights the innovative approach and findings of the recently published study by Xu et al,which underscores the integration of radiomics and clinicoradiological factors to enhance the preoperative prediction of microvascular invasion in patients with hepatitis B virus-related hepatocellular carcinoma(HBV-HCC).The study’s use of contrast-enhanced computed tomography radiomics to construct predictive models offers a significant advancement in the surgical planning and management of HBV-HCC,potentially transforming patient outcomes through more personalized treatment strategies.This editorial commends the study's contribution to precision medicine and discusses its implic-ations for future research and clinical practice.
基金supported by National Natural Science Foundation of China(Nos.22122803 and 21788102)the National Natural Science Foundation of Jiangsu Province(No.BK20220644).
文摘Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associatedβ-galactosidase(SA-β-Gal),which is proved to be closely associated with senescent cells,the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associatedβ-Gal(OA-β-Gal)will interfere with imaging accuracy.Herein,a near-infrared(NIR)hemicyanine based fluorescent probe HCyXA-βGal was designed for light-up imaging of live cells containingβ-Gal.With the organelle-targeting morpholinyl and positive charge moieties,HCyxA-βGal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells.Furthermore,inspired by the fast response performance,fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal:time-dependent intensity.
基金support from the National Natural Science Foundation of China(No.22274005,U2167222)the Science Fund for Creative Research Groups of Nature Science Foundation of Hebei Province(B2021201038)+4 种基金Natural Science Foundation of Hebei Province(B2023201108)Hebei Province Higher Education Science and Technology Research Project(JZX2023001)Research and Innovation Team of Hebei University(IT2023A01)Hebei Province Innovation Capability Enhancement Plan Project(22567632H)Open Research Fund of School of Chemistry and Chemical Engineering,Henan Normal University.
文摘In situ precise detection of bioactive molecules with high sensitivity and spatiotemporal resolution is essential for studying physiological events and disease diagnosis.The utilization of versatile fluorescent probes in fluorescence imaging offers a powerful tool for in vivo imaging of biomarkers closely associated with pathological conditions.However,the dynamic behavior leading to rapid clearance of small molecule probes from regions of interest severely compromises their potential for precise imaging.Notably,self-immobilizing fluorescent probes that selectively recognize diseased tissues while improving in situ retention and enrichment enable accurate high-fidelity fluorescence imaging.In this review,we aim to summarize the strategies employed for recent advances in the performance and precision of in vivo fluorescence imaging using self-immobilizing techniques.Lastly,we discuss the prospects and potential challenges associated with selfimmobilizing fluorescent probes to promote further development and application of more delicate fluorescent probes.
基金funded by the Canada Foundation for Innovation the Ontario Ministry of Research and Innovation,and Industry Canada.
文摘Atomic force microscope(AFM)systems rely on silicon(Si)probes for precise nanoscale characterization across diverse environments.However,fabricating high-aspect-ratio(HAR)and sharp Si tips and optimizing the handle geometries remain significant challenges.Conventional HAR probe fabrication methods lack scalability,precision,and cost efficiency,while cuboid-shaped handles risk obstructing laser detection and limiting compatibility.This study presents an innovative batch-fabrication strategy for high-performance Si AFM probes that integrate ultra-sharp HAR tips,rectangular cantilevers,and universally compatible stair-shaped handles.Notably,the tip fabrication process employs only low-cost microscale ultraviolet(UV)lithography,while still achieving nanoscale structural resolution.The fabricated probes exhibit a tip apex radius of 5 nm and a half-cone angle of 7.5°,enabling high-resolution and highfidelity imaging.The novel stair-shaped handle geometry is introduced and fabricated via a single-step dry etching process,which provides unobstructed laser detection and ensures compatibility with a broad range of commercial AFM platforms.Durability testing demonstrates stable scanning performance for up to 8 hours within the 100 nm precision range,confirming the mechanical reliability of the design.This scalable,reproducible,and high-yield fabrication strategy represents a significant advancement in HAR AFM probe development,providing enhanced performance and extended applicability for diverse nanoscale imaging applications.
基金support by the National Natural Science Foundation of China(32171429)the Natural Science Foundation of Hunan Province(2022JJ30672,2024JJ9218).
文摘Molecular imaging has emerged as a transformative tool in cancer diagnosis,enabling the visualization of biological processes at the cellular and molecular levels.Aptamers,single-stranded oligonucleotides with high affinity and specificity for target molecules,have gained significant attention as versatile probes for molecular imaging due to their unique properties,including small size,ease of modification,low immunogenicity,and rapid tissue penetration.This review explores the integration of aptamers with various imaging agents to enhance cancer diagnosis and therapy.Aptamer-based imaging probes offer high sensitivity and real-time visualization of tumor markers.Aptamer-based fluorescence probes and aptamer-conjugated magnetic resonance imaging(MRI)probes,including gadolinium-based contrast agents,improve tumor targeting and imaging resolution.Additionally,aptamers have been utilized in single-photon emission computed tomography(SPECT)and positron emission tomography(PET)imaging to enhance the specificity of radiotracers for cancer detection.Furthermore,aptamer-targeted ultrasound and computed tomography(CT)imaging demonstrate the potential for noninvasive and precise tumor localization.By leveraging the unique advantages of aptamers,these imaging strategies not only improve diagnostic accuracy but also pave the way for image-guided cancer therapies.This review highlights the significant role of aptamers in advancing molecular imaging and their potential to revolutionize cancer diagnosis and treatment.
基金supported by National Key R&D Program of China(No.2023YFF071)the National Natural Science Foundation of China(Nos.92163214,52333003 and 52373288)+1 种基金the Basic Research Program of Jiangsu(BK20244003)the Natural Science Foundation of Jiangsu Province(No.BK20202002).
文摘Nanobodies,single-domain antibodies derived from camelid heavy-chain antibodies,have emerged as powerful molecular tools in cancer diagnosis and therapy due to their unique structural and functional attributes,including small size,high antigen specificity,robust stability,and ease of genetic and chemical modification.This Review provides an extensive overview of the nanobody structure,characteristics,and production methods,highlighting their advantages over conventional antibodies.We comprehensively discuss various applications of nanobodies in cancer diagnosis and therapy,encompassing their roles as antagonists or agonists in modulating oncogenic and immune-related signaling pathways,targeted delivery systems for chemotherapeutics,radionuclides,immunotoxins,and photo-sensitizers,and their integration into advanced imaging technologies such as PET,SPECT,and optical imaging for accurate tumor detection and monitoring.Additionally,we explore the development of innovative cell-based immunotherapies leveraging nanobody-engineered chimeric antigen receptor(CAR)-modified T cells,natural killer cells,and macrophages,illustrating their potential to enhance therapeutic precision and efficacy.Finally,we discuss the current challenges and provide perspectives on emerging nanobody technologies and their translational prospects,underscoring the potential of nanobody-based toolkits to drive next-generation precision cancer diagnostics and therapies.
基金supported by National Basic Research Program of China(Grant No. 2012CB719906)
文摘This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth observation program. In addition, this paper expounded the transformation mechanism and procedure from earth observation data to geospatial information and geographical knowledge, and examined the key scientific and technological issues, including earth observation networks, high-precision image positioning, image understanding, automatic spatial information extraction, and focus services. These analyses provide a new impetus for pushing the application of China's high-resolution earth observation system from a "quantity" to "quality" change, from China to the world, from providing products to providing online service.
