Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals...Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge challenge.In this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMPs,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first time.Benefit from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMPs,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported biomaterials.Leveraging the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum samples.We also established an LC-MS/MS method using the MMPs for tracking rituximab biotransformation in patient serum.Intriguingly,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful monitoring.Overall,these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.展开更多
Since the Reform and Opening-up, remarkable achievements in poverty alleviation have been made in China. China's success in mass poverty reduction can be attributed to its rapid economic growth, large-scale region...Since the Reform and Opening-up, remarkable achievements in poverty alleviation have been made in China. China's success in mass poverty reduction can be attributed to its rapid economic growth, large-scale regional poverty alleviation and development, sound social security policies, widereaching farmer-benefiting policies and the equal land allotment system. However, with income inequality on the rise, poverty reduction effect made by the economic growth is declining: More targeted poverty alleviation policies are needed by China. Targeted poverty alleviation aims to improve the effect and efficiency of poverty alleviation through precise identification of poverty-stricken populations and comprehensive supportive measures. To tackle a variety of challenges such as the poor cultivation among povertystricken populations, the complex causes for poverty, and inflexible capital management, China should improve its poverty alleviation mechanism by innovating poverty identification methods, support approaches, capital management and performance assessment.展开更多
Molecular imaging was developed from basic molecular recognition.It can visualize not only the expression levels of specific molecules in a living system but also specific biological processes,thus providing guidance ...Molecular imaging was developed from basic molecular recognition.It can visualize not only the expression levels of specific molecules in a living system but also specific biological processes,thus providing guidance for early detection and treatment of diseases.As a noninvasive method,imaging agents are one of the foundations of high spatial resolution imaging,and their sensitivity and specificity can be improved by coupling targeting ligands to imaging probes.Among the various targeting ligands(antibodies,aptamers,etc.),targeting peptides are widely used in various modalities of molecular imaging due to their high affinities toward the molecular target and their excellent physicochemical properties.In this review,we summarize the design concepts and methods of targeting peptides in molecular imaging,introduce the combination of targeting peptides and imaging probes in different imaging modalities(e.g.,fluorescence imaging,radionuclide imaging),and provide examples of their applications in bioimaging.Finally,the challenges and strategies for clinical translation and practical application of targeting peptide-based imaging reagents are briefly discussed.展开更多
基金supported by the National Natural Science Foundation of China(82173773,82273893,82373829)the Natural Science Foundation of Guangdong Province,China(2021A0505030039,2021A0505020014)+1 种基金the High-End Foreign Experts Project,China(G2021199005L)the Science and Technology Program of Guangdong Provincial Medical Products Administration,China(2023TDZ11)。
文摘Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge challenge.In this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMPs,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first time.Benefit from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMPs,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported biomaterials.Leveraging the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum samples.We also established an LC-MS/MS method using the MMPs for tracking rituximab biotransformation in patient serum.Intriguingly,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful monitoring.Overall,these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.
基金"Studies in the Mechanism and Policies on Targeted Poverty Alleviation and Elimination"(15ZDC026)-a major program of the National Social Sciences Fund
文摘Since the Reform and Opening-up, remarkable achievements in poverty alleviation have been made in China. China's success in mass poverty reduction can be attributed to its rapid economic growth, large-scale regional poverty alleviation and development, sound social security policies, widereaching farmer-benefiting policies and the equal land allotment system. However, with income inequality on the rise, poverty reduction effect made by the economic growth is declining: More targeted poverty alleviation policies are needed by China. Targeted poverty alleviation aims to improve the effect and efficiency of poverty alleviation through precise identification of poverty-stricken populations and comprehensive supportive measures. To tackle a variety of challenges such as the poor cultivation among povertystricken populations, the complex causes for poverty, and inflexible capital management, China should improve its poverty alleviation mechanism by innovating poverty identification methods, support approaches, capital management and performance assessment.
基金support from Beijing Natural Science Foundation(No.2222029)National Natural Science Foundation of China(Nos.22322403 and 22074006)Beijing Institute of Technology Research Fund Program for Young Scholars.
文摘Molecular imaging was developed from basic molecular recognition.It can visualize not only the expression levels of specific molecules in a living system but also specific biological processes,thus providing guidance for early detection and treatment of diseases.As a noninvasive method,imaging agents are one of the foundations of high spatial resolution imaging,and their sensitivity and specificity can be improved by coupling targeting ligands to imaging probes.Among the various targeting ligands(antibodies,aptamers,etc.),targeting peptides are widely used in various modalities of molecular imaging due to their high affinities toward the molecular target and their excellent physicochemical properties.In this review,we summarize the design concepts and methods of targeting peptides in molecular imaging,introduce the combination of targeting peptides and imaging probes in different imaging modalities(e.g.,fluorescence imaging,radionuclide imaging),and provide examples of their applications in bioimaging.Finally,the challenges and strategies for clinical translation and practical application of targeting peptide-based imaging reagents are briefly discussed.
