Aiming at improving the seed spacing uniformity of maize planter at high forward speed,a seed precise delivery mechanism driven by electric motor has been developed and evaluated in laboratory.The mechanism was design...Aiming at improving the seed spacing uniformity of maize planter at high forward speed,a seed precise delivery mechanism driven by electric motor has been developed and evaluated in laboratory.The mechanism was designed to deliver single seed from seed meter to furrow.Seed’s movement from the seed release point in a seed meter to the seed delivery cavity in the belt was analyzed,the mathematical model of seed movement in the seed precise delivery mechanism was established based on seed delivery time analysis.A mechanical prototype was designed and associated control system was developed.The performance contrast experiment was conducted between a traditional seed tube and newly developed seed precise delivery mechanism,and test results indicated that the qualities of feed index of the seed precise delivery mechanism were higher than those of the traditional seed tube delivery mechanism,and the coefficients of variation of the seed precise delivery mechanism were lower than those of the traditional seed tube delivery mechanism when the forward speed of planter was set at 10 km/h,12 km/h and 14 km/h,respectively.It indicated the seed precise delivery mechanism significantly improved seed spacing uniformity of maize planter compared with the traditional seed tube delivery mechanism.展开更多
Long non-coding RNAs(lncRNAs)play pivotal roles in the regulation of gene expression,particularly in maintaining pluripotency and directing stem cell.By orchestrating stem cell fate decisions and lineage commitment th...Long non-coding RNAs(lncRNAs)play pivotal roles in the regulation of gene expression,particularly in maintaining pluripotency and directing stem cell.By orchestrating stem cell fate decisions and lineage commitment through epigenetic,transcriptional,and post-transcriptional mechanisms,lncRNAs have emerged as key modulators in developmental biology.Their therapeutic potential has garnered increasing interest,especially in the contexts of regenerative medicine,disease modeling,targeted delivery systems,and precision therapeutics.This review presents a comprehensive overview of the mechanisms by which lncRNAs govern stem cell differentiation and examines emerging lncRNA-based therapeutic strategies,emphasizing major challenges and prospective research directions in this rapidly advancing field.展开更多
Thrombosis is a leading cause of mortality worldwide.As important gaseous signaling molecules,both nitric oxide(NO)and hydrogen sulfide(H_(2)S)demonstrate antiplatelet and anticoagulant functions,but little attention ...Thrombosis is a leading cause of mortality worldwide.As important gaseous signaling molecules,both nitric oxide(NO)and hydrogen sulfide(H_(2)S)demonstrate antiplatelet and anticoagulant functions,but little attention has been given to their synergistic effect and the underlying mechanism.In the present study,we developed an NO/H_(2)S codelivery system based on enzyme prodrug therapy(EPT)strategy in which the prodrugs are specif-ically recognized by the engineeredβ-galactosidase.Targeted codelivery of NO and H_(2)S in vivo was demonstrated by near-infrared fluorescence imaging and confirmed by measuring plasma and tissue levels;as a result,the side effects caused by systemic delivery,such as bleeding time,were reduced.Delivery of an optimized combination of NO and H_(2)S with a low combination index(CI)results in a synergistic effect on the inhibition of platelet adhesion and activation.Mechanistically,NO and H_(2)S cooperatively enhance the cGMP level through redoxbased posttranslational modifications of phosphodiesterase 5A(PDE5A),which leads to activation of the cGMP/PKG signaling pathway.Furthermore,targeted codelivery of NO and H_(2)S demonstrates enhanced thera-peutic efficacy for thrombosis in two mouse models of FeCl_(3)-induced arterial thrombosis and deep vein thrombosis.Collectively,these results confirm the synergistic efficacy of NO and H_(2)S for antithrombotic therapy,and the codelivery system developed in this study represents a promising candidate for clinical translation.展开更多
Particle formulation engineering stands as a focal point of research and a critical trajectory within the chemical industry.In response to the challenges associated with antigen/drug delivery,our research group has pr...Particle formulation engineering stands as a focal point of research and a critical trajectory within the chemical industry.In response to the challenges associated with antigen/drug delivery,our research group has proposed a suite of strategies centered on micro/nanoparticle platforms.This review integrates our investigations into the applications of particles across various dimensions in biomedical delivery systems.Specifically,it delineates the mechanisms by which particles augment vaccine-induced immune responses,notably through antigen cross-presentation,and the pivotal roles they play in facilitating drug-mediated targeting of cancer cells via confined mass transfer.This review also encompasses recent advancements in particle formulations,offering prospective insights into the utilization of chemical engineering principles in the design of nextgeneration biomedical delivery systems.展开更多
The meticulous regulation of mechanochemical activation in the depths of tissue using non-invasive ultrasound technology harbors significant potential to enhance our comprehension of core biomedical sciences and to tr...The meticulous regulation of mechanochemical activation in the depths of tissue using non-invasive ultrasound technology harbors significant potential to enhance our comprehension of core biomedical sciences and to transform the landscape of disease treatment methodologies[1].Despite this promise,the scientific community has yet to uncover a mechanoresponsive materials system that is guided by robust theoretical frameworks and characterized by clearly defined ultrasound activation parameters.Such a system would not only deepen our understanding of the intricate interplay between mechanical forces and biological responses but also pave the way for innovative therapeutic strategies that could be precisely controlled and tailored to individual patient needs.The exploration of this uncharted territory stands as a beacon,calling for interdisciplinary collaboration to unlock the full potential of ultrasound in medical science and treatment paradigms.展开更多
基金The authors disclosed receipt of the following financial support for the research,authorship,and/or publication of this article:This work was funded by the National Natural Science Foundation of China(Grant No.51575515)the National key research and development program in 13th Five-Year(Grant No.2017YFD0700703)the Soil-Machine-Plant Key Laboratory of the Ministry of Agriculture of PR China and Project of the Modern National Industry System of Maize Industrial Technology(CARS-02).
文摘Aiming at improving the seed spacing uniformity of maize planter at high forward speed,a seed precise delivery mechanism driven by electric motor has been developed and evaluated in laboratory.The mechanism was designed to deliver single seed from seed meter to furrow.Seed’s movement from the seed release point in a seed meter to the seed delivery cavity in the belt was analyzed,the mathematical model of seed movement in the seed precise delivery mechanism was established based on seed delivery time analysis.A mechanical prototype was designed and associated control system was developed.The performance contrast experiment was conducted between a traditional seed tube and newly developed seed precise delivery mechanism,and test results indicated that the qualities of feed index of the seed precise delivery mechanism were higher than those of the traditional seed tube delivery mechanism,and the coefficients of variation of the seed precise delivery mechanism were lower than those of the traditional seed tube delivery mechanism when the forward speed of planter was set at 10 km/h,12 km/h and 14 km/h,respectively.It indicated the seed precise delivery mechanism significantly improved seed spacing uniformity of maize planter compared with the traditional seed tube delivery mechanism.
基金Supported by the National Natural Science Foundation of China,No.32200755 and No.32200621the Natural Science Foundation of Gansu Province,No.23JRRA696。
文摘Long non-coding RNAs(lncRNAs)play pivotal roles in the regulation of gene expression,particularly in maintaining pluripotency and directing stem cell.By orchestrating stem cell fate decisions and lineage commitment through epigenetic,transcriptional,and post-transcriptional mechanisms,lncRNAs have emerged as key modulators in developmental biology.Their therapeutic potential has garnered increasing interest,especially in the contexts of regenerative medicine,disease modeling,targeted delivery systems,and precision therapeutics.This review presents a comprehensive overview of the mechanisms by which lncRNAs govern stem cell differentiation and examines emerging lncRNA-based therapeutic strategies,emphasizing major challenges and prospective research directions in this rapidly advancing field.
基金supported by grants from the National Natural Sci-ence Foundation of China(Nos.81925021,82330066,U21A20391,823B2048,82370343)Natural Science Foundation of Tianjin of China and the Natural Science Foundation of Heilongjiang Province of China(ZD2023H005).
文摘Thrombosis is a leading cause of mortality worldwide.As important gaseous signaling molecules,both nitric oxide(NO)and hydrogen sulfide(H_(2)S)demonstrate antiplatelet and anticoagulant functions,but little attention has been given to their synergistic effect and the underlying mechanism.In the present study,we developed an NO/H_(2)S codelivery system based on enzyme prodrug therapy(EPT)strategy in which the prodrugs are specif-ically recognized by the engineeredβ-galactosidase.Targeted codelivery of NO and H_(2)S in vivo was demonstrated by near-infrared fluorescence imaging and confirmed by measuring plasma and tissue levels;as a result,the side effects caused by systemic delivery,such as bleeding time,were reduced.Delivery of an optimized combination of NO and H_(2)S with a low combination index(CI)results in a synergistic effect on the inhibition of platelet adhesion and activation.Mechanistically,NO and H_(2)S cooperatively enhance the cGMP level through redoxbased posttranslational modifications of phosphodiesterase 5A(PDE5A),which leads to activation of the cGMP/PKG signaling pathway.Furthermore,targeted codelivery of NO and H_(2)S demonstrates enhanced thera-peutic efficacy for thrombosis in two mouse models of FeCl_(3)-induced arterial thrombosis and deep vein thrombosis.Collectively,these results confirm the synergistic efficacy of NO and H_(2)S for antithrombotic therapy,and the codelivery system developed in this study represents a promising candidate for clinical translation.
基金supported by the National Natural Science Foundation of China(Grant Nos.82341405,32030062,and U20A20361)the National Key Research and Development Program of China(Grant Nos.2021YFC2302603 and 2023YFC2307700)+1 种基金the CAS Project for Young Scientists in Basic Research(Grant No.YSBR-083)the IPE Project for Frontier Basic Research(Grant No.QYJC-2022-012).
文摘Particle formulation engineering stands as a focal point of research and a critical trajectory within the chemical industry.In response to the challenges associated with antigen/drug delivery,our research group has proposed a suite of strategies centered on micro/nanoparticle platforms.This review integrates our investigations into the applications of particles across various dimensions in biomedical delivery systems.Specifically,it delineates the mechanisms by which particles augment vaccine-induced immune responses,notably through antigen cross-presentation,and the pivotal roles they play in facilitating drug-mediated targeting of cancer cells via confined mass transfer.This review also encompasses recent advancements in particle formulations,offering prospective insights into the utilization of chemical engineering principles in the design of nextgeneration biomedical delivery systems.
文摘The meticulous regulation of mechanochemical activation in the depths of tissue using non-invasive ultrasound technology harbors significant potential to enhance our comprehension of core biomedical sciences and to transform the landscape of disease treatment methodologies[1].Despite this promise,the scientific community has yet to uncover a mechanoresponsive materials system that is guided by robust theoretical frameworks and characterized by clearly defined ultrasound activation parameters.Such a system would not only deepen our understanding of the intricate interplay between mechanical forces and biological responses but also pave the way for innovative therapeutic strategies that could be precisely controlled and tailored to individual patient needs.The exploration of this uncharted territory stands as a beacon,calling for interdisciplinary collaboration to unlock the full potential of ultrasound in medical science and treatment paradigms.
