Although prebiotic activities of alginate and agar oligosaccharides isolated from seaweeds have been reported, it remains unknown whether seaweed polysaccharides have prebiotic activity. In this study, we isolated pol...Although prebiotic activities of alginate and agar oligosaccharides isolated from seaweeds have been reported, it remains unknown whether seaweed polysaccharides have prebiotic activity. In this study, we isolated polysaccharides from four species of seaweeds, such as Grateloupia fi licina(GFP), Eucheuma spinosum(ESP), Ulva pertusa(UPP), and A scophyllum nodosum(ANP), and characterized their structures and prebiotic ef fects in vitro. The results showed that these polysaccharides were dif ferent in total sugar and sulfate contents as well as monosaccharide composition. GFP and ESP significantly promoted bifi dobacterium proliferation and 0.1% ESP and 0.4% GFP resulted in the highest proliferation rates of beneficial bacteria, whereas UPP and ANP inhibited the growth of beneficial bacteria at all tested concentrations(0.1%–0.5%). The different behaviors of the four seaweed-originated polysaccharides might be refl ected by differences in monosaccharide composition and structure. Therefore, polysaccharides isolated from GFP and ESP could be utilized as prebiotics. However, more studies must be carried out in vivo.展开更多
Sialylated immunoglobulin G(IgG)is a kind of glycoproteins in breast milk with the property of anti-inflammation.Our previous work showed that sialylated IgG could significantly promote the growth of Bifidobacterium i...Sialylated immunoglobulin G(IgG)is a kind of glycoproteins in breast milk with the property of anti-inflammation.Our previous work showed that sialylated IgG could significantly promote the growth of Bifidobacterium in the gut microbiota of healthy volunteers.Nevertheless,whether sialylated IgG can benefit patients with inflammatory bowel disease(IBD)as prebiotics remains unclear.Therefore,its prebiotic effect on the gut microbiota of IBD patients was investigated by in vitro fermentation in this study.The results showed that sialylated IgG could significantly enhance the diversity,richness and composition of gut microbiota in IBD samples compared with non-sialylated IgG.In detail,sialylated IgG not only improved the growth of three common genera(Romboutsia,Prevotella and Akkermansia)with low relative abundance in IBD samples but also increased the genera(Bifidobacterium,Actinomyces,Atopobium and Citrobacter)with low relative abundance both in healthy and IBD fecal samples.Correspondingly,the contents of short-chain fatty acids for fermentation group of fecal sample from IBD patients with sialylated IgG as the carbon source were higher than those for fermentation group of fecal sample from IBD patients with non-sialylated IgG as the carbon source.In addition,the metabolic pathway results confirmed that sialylated IgG upregulated the functional genes related to hydrolysis,absorption and utilization of carbohydrates.The transcriptome sequencing results further showed that sialylated IgG could induce particular gene expression of Bifidobacterium bifidum to modulate the gut microbiota of IBD patients.Thus,as a kind of natural dietary component,sialylated IgG has potential applications in treating IBD.展开更多
In this study, bio-yogurts, which contain L. acidophilus and Bifidobacterium animalis subsp. lactis, were produced with cellulose fiber (CF) addition at a various level (0%, 0.5%, 1% and 2%) and stored at 4°C ...In this study, bio-yogurts, which contain L. acidophilus and Bifidobacterium animalis subsp. lactis, were produced with cellulose fiber (CF) addition at a various level (0%, 0.5%, 1% and 2%) and stored at 4°C ± 1°C for 20 days. Bio-yogurts were analysed 1, 10 and 20 days after production. The storage period significantly influenced overall properties of the samples. Addition of CF has positively affected the physical and textural properties of yoghurt, such as serum separation, water holding capacity, viscosity, firmness, adhesiveness, cohesiveness, springiness, gumminess and chewiness (p < 0.05). It also slightly stimulated the development of probiotic bacteria (p < 0.05). Bio-yogurt, which contains 0.5% CF in sensory aspect, is the most appreciated.展开更多
The objectives of this research work were to evaluate the effect of in vitro gastrointestinal digestion (GIT) on melon peel juice (MPJ) powder from fruit processing industry by-products,considering (i) the recovery an...The objectives of this research work were to evaluate the effect of in vitro gastrointestinal digestion (GIT) on melon peel juice (MPJ) powder from fruit processing industry by-products,considering (i) the recovery and accessibility indexes,(ii) the changes on antioxidant activity,and (iii) the prebiotic effect.Throughout exposition to GIT conditions a decrease on the total phenolic content (TPC = 65.31%) and antioxidant activity by ABTS = 39.77% and DPPH = 45.91% were observed.However,these both parameters exhibited stable accessibility,accounting with 81.89%,76.55%,and 54.07% for TPC,ABTS and DPPH,respectively.After gastrointestinal digestion,the non-absorbed fraction exhibited a positive impact on the growth of Bifidobacterium and Lactobacillus strains,possibly associated with the high content of simple sugar (glucose and fructose).This fraction also showed to be safe on Caco-2 intestinal cells.These findings suggest that MPJ might be used as a potential food functional ingredient.展开更多
基金Supported by the Commonwealth Item of the State Oceanic Administration of the People’s Republic of China(Nos.201405038-2,201505033)Qingdao People’s Livelihood Science and Technology Projects(No.16-6-2-41-nsh)
文摘Although prebiotic activities of alginate and agar oligosaccharides isolated from seaweeds have been reported, it remains unknown whether seaweed polysaccharides have prebiotic activity. In this study, we isolated polysaccharides from four species of seaweeds, such as Grateloupia fi licina(GFP), Eucheuma spinosum(ESP), Ulva pertusa(UPP), and A scophyllum nodosum(ANP), and characterized their structures and prebiotic ef fects in vitro. The results showed that these polysaccharides were dif ferent in total sugar and sulfate contents as well as monosaccharide composition. GFP and ESP significantly promoted bifi dobacterium proliferation and 0.1% ESP and 0.4% GFP resulted in the highest proliferation rates of beneficial bacteria, whereas UPP and ANP inhibited the growth of beneficial bacteria at all tested concentrations(0.1%–0.5%). The different behaviors of the four seaweed-originated polysaccharides might be refl ected by differences in monosaccharide composition and structure. Therefore, polysaccharides isolated from GFP and ESP could be utilized as prebiotics. However, more studies must be carried out in vivo.
基金The study was supported by the National Key Research and Development Program of China(2017YFD0400600)the National Natural Science Foundation of China(No.31901617)+5 种基金the Top Talent Project of Department of Education of Anhui Province(gxyqZD2021125)the Excellent Talent project of Anhui Science and Technology University(XJYXRC202101)the Stable Talent Personnel Project of Anhui Science and Technology University(SPWD202101)the Graduate Innovation and Entrepreneurship Training Program of Anhui Province(2022cxcysj199)the Natural Science Research Foundation of Department of Education of Anhui Province(2022AH040236)the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘Sialylated immunoglobulin G(IgG)is a kind of glycoproteins in breast milk with the property of anti-inflammation.Our previous work showed that sialylated IgG could significantly promote the growth of Bifidobacterium in the gut microbiota of healthy volunteers.Nevertheless,whether sialylated IgG can benefit patients with inflammatory bowel disease(IBD)as prebiotics remains unclear.Therefore,its prebiotic effect on the gut microbiota of IBD patients was investigated by in vitro fermentation in this study.The results showed that sialylated IgG could significantly enhance the diversity,richness and composition of gut microbiota in IBD samples compared with non-sialylated IgG.In detail,sialylated IgG not only improved the growth of three common genera(Romboutsia,Prevotella and Akkermansia)with low relative abundance in IBD samples but also increased the genera(Bifidobacterium,Actinomyces,Atopobium and Citrobacter)with low relative abundance both in healthy and IBD fecal samples.Correspondingly,the contents of short-chain fatty acids for fermentation group of fecal sample from IBD patients with sialylated IgG as the carbon source were higher than those for fermentation group of fecal sample from IBD patients with non-sialylated IgG as the carbon source.In addition,the metabolic pathway results confirmed that sialylated IgG upregulated the functional genes related to hydrolysis,absorption and utilization of carbohydrates.The transcriptome sequencing results further showed that sialylated IgG could induce particular gene expression of Bifidobacterium bifidum to modulate the gut microbiota of IBD patients.Thus,as a kind of natural dietary component,sialylated IgG has potential applications in treating IBD.
文摘In this study, bio-yogurts, which contain L. acidophilus and Bifidobacterium animalis subsp. lactis, were produced with cellulose fiber (CF) addition at a various level (0%, 0.5%, 1% and 2%) and stored at 4°C ± 1°C for 20 days. Bio-yogurts were analysed 1, 10 and 20 days after production. The storage period significantly influenced overall properties of the samples. Addition of CF has positively affected the physical and textural properties of yoghurt, such as serum separation, water holding capacity, viscosity, firmness, adhesiveness, cohesiveness, springiness, gumminess and chewiness (p < 0.05). It also slightly stimulated the development of probiotic bacteria (p < 0.05). Bio-yogurt, which contains 0.5% CF in sensory aspect, is the most appreciated.
基金National Council of Science and Technology(CONACyT,Mexico)for PhD fellowship support granted to G´omez-García Ricardothe project MultiBiorefinery:Estrat´egias multiuso para a valorizaç˜ao de uma gama alargada de subprodutos agroflorestais e das pescas:Um passo em frente na criaç˜ao de uma biorrefinaria"financiado pelo Programa Operacional Competitividade e Internacionalizaç˜ao(POCI-01-0145-FEDER-016403)e pelo Programa Operacional Regional de Lisboa(LISBOA-01-0145-FEDER-016403)na sua componente FEDER e pela Fundaç˜ao para a Ciˆencia e Tecnologia,I.P.na componente nacional(SAICTPAC/0040/2015).
文摘The objectives of this research work were to evaluate the effect of in vitro gastrointestinal digestion (GIT) on melon peel juice (MPJ) powder from fruit processing industry by-products,considering (i) the recovery and accessibility indexes,(ii) the changes on antioxidant activity,and (iii) the prebiotic effect.Throughout exposition to GIT conditions a decrease on the total phenolic content (TPC = 65.31%) and antioxidant activity by ABTS = 39.77% and DPPH = 45.91% were observed.However,these both parameters exhibited stable accessibility,accounting with 81.89%,76.55%,and 54.07% for TPC,ABTS and DPPH,respectively.After gastrointestinal digestion,the non-absorbed fraction exhibited a positive impact on the growth of Bifidobacterium and Lactobacillus strains,possibly associated with the high content of simple sugar (glucose and fructose).This fraction also showed to be safe on Caco-2 intestinal cells.These findings suggest that MPJ might be used as a potential food functional ingredient.
