子痫前期(preeclampsia,PE)是一种严重的妊娠期并发症,其主要症状包括高血压和多系统器官损害,严重影响母儿健康。终止妊娠是目前治疗PE的首选方法。尽管PE的发病机制尚未完全明确,但近年研究表明,不同来源的外泌体(exosomes)及其携带...子痫前期(preeclampsia,PE)是一种严重的妊娠期并发症,其主要症状包括高血压和多系统器官损害,严重影响母儿健康。终止妊娠是目前治疗PE的首选方法。尽管PE的发病机制尚未完全明确,但近年研究表明,不同来源的外泌体(exosomes)及其携带的微小RNA(microRNA,miRNA)在PE的发展中发挥关键作用,尤其是miRNA作为外泌体小RNA中最具代表性的一种,可通过调节滋养细胞功能和信号通路参与PE的病理生理过程。特别是,外泌体miRNA能够抑制细胞因子信号传导抑制因子1(suppressor of cytokine signaling 1,SOCS1),从而调节母体的免疫反应。此外,外泌体miRNA在PE的早期诊断中显示出潜力,有望成为预测PE的生物标志物。展开更多
Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs wer...Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.展开更多
目的:探讨血清 miR- 210和 miR- 155水平对先兆子痫(PE)早期诊断的临床价值。方法:选取PE孕妇(PE组)和健康孕妇(对照组)各60例,收集所有孕妇的临床资料(年龄、孕周、身体质量指数、收缩压、舒张压、血红蛋白、尿素氮和肌酐等),采用实时...目的:探讨血清 miR- 210和 miR- 155水平对先兆子痫(PE)早期诊断的临床价值。方法:选取PE孕妇(PE组)和健康孕妇(对照组)各60例,收集所有孕妇的临床资料(年龄、孕周、身体质量指数、收缩压、舒张压、血红蛋白、尿素氮和肌酐等),采用实时定量荧光(PCR)法检测孕妇血清 miR -210和 miR -155的相对表达水平,采用接受者操作特性曲线( ROC )分析候选 mi RNA作为PE潜在诊断标志物的准确性。结果:两组孕妇的年龄、身体质量指数(BMI)、孕周、血红蛋白和尿素氮差异无统计学意义( P >0.05),PE组孕妇收缩压、舒张压和肌酐水平均明显高于对照组孕妇,差异有高度统计学意义( P <0.01);PE组孕妇血清 miR -210和 miR -155水平均高于对照组孕妇,差异有统计学意义( P <0.05);孕妇血清 miR -210的 ROC 曲线下面积为0.904(95% CI为 0.847~0.961, P <0.01), miR -155的 ROC 曲线下面积为0.843(95% CI 为0.766~0.920, P <0.01),孕妇血清 miR -210水平用于预测PE的灵敏度和特异性均高于 miR -155。结论:孕妇血清 miR -210和 miR -155水平可为PE的早期诊断提供参考, miR -210的诊断价值高于 miR -155。展开更多
文摘子痫前期(preeclampsia,PE)是一种严重的妊娠期并发症,其主要症状包括高血压和多系统器官损害,严重影响母儿健康。终止妊娠是目前治疗PE的首选方法。尽管PE的发病机制尚未完全明确,但近年研究表明,不同来源的外泌体(exosomes)及其携带的微小RNA(microRNA,miRNA)在PE的发展中发挥关键作用,尤其是miRNA作为外泌体小RNA中最具代表性的一种,可通过调节滋养细胞功能和信号通路参与PE的病理生理过程。特别是,外泌体miRNA能够抑制细胞因子信号传导抑制因子1(suppressor of cytokine signaling 1,SOCS1),从而调节母体的免疫反应。此外,外泌体miRNA在PE的早期诊断中显示出潜力,有望成为预测PE的生物标志物。
基金supported by the National Natural Science Foundation of China (No.30830080 and 30800779)the Natural Science Foundation of Beijing (No.5072035)the Ministry of Science and Technology of China (No.2006AA10Z135,2008AA10Z143,2006CB102105 and 2009CB941604)
文摘Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.
文摘目的:探讨血清 miR- 210和 miR- 155水平对先兆子痫(PE)早期诊断的临床价值。方法:选取PE孕妇(PE组)和健康孕妇(对照组)各60例,收集所有孕妇的临床资料(年龄、孕周、身体质量指数、收缩压、舒张压、血红蛋白、尿素氮和肌酐等),采用实时定量荧光(PCR)法检测孕妇血清 miR -210和 miR -155的相对表达水平,采用接受者操作特性曲线( ROC )分析候选 mi RNA作为PE潜在诊断标志物的准确性。结果:两组孕妇的年龄、身体质量指数(BMI)、孕周、血红蛋白和尿素氮差异无统计学意义( P >0.05),PE组孕妇收缩压、舒张压和肌酐水平均明显高于对照组孕妇,差异有高度统计学意义( P <0.01);PE组孕妇血清 miR -210和 miR -155水平均高于对照组孕妇,差异有统计学意义( P <0.05);孕妇血清 miR -210的 ROC 曲线下面积为0.904(95% CI为 0.847~0.961, P <0.01), miR -155的 ROC 曲线下面积为0.843(95% CI 为0.766~0.920, P <0.01),孕妇血清 miR -210水平用于预测PE的灵敏度和特异性均高于 miR -155。结论:孕妇血清 miR -210和 miR -155水平可为PE的早期诊断提供参考, miR -210的诊断价值高于 miR -155。
