Three crucial hurdles hinder studies on human cytomegalovirus(HCMV): strict species specificity, differences between in vivo and in vitro infection, and the complexity of gene regulation. Ever since the sequencing of ...Three crucial hurdles hinder studies on human cytomegalovirus(HCMV): strict species specificity, differences between in vivo and in vitro infection, and the complexity of gene regulation. Ever since the sequencing of the whole genome was first accomplished, functional studies on individual genes have been the mainstream in the CMV field. Gene regulation has therefore been elucidated in a more detailed fashion. However, viral gene regulation is largely controlled by both cellular and viral components. In other words, viral gene expression is determined by the virus–host interaction. Generally, cells respond to viral infection in a defensive pattern; at the same time, viruses try to counteract the cellular defense or else hide in the host(latency). Viruses evolve effective strategies against cellular defense in order to achieve replicative success. Whether or not they are successful, cellular defenses remain in the whole viral replication cycle: entry, immediate–early(IE) gene expression, early gene expression, DNA replication, late gene expression, and viral egress. Many viral strategies against cellular defense, and which occur in the immediate–early time of viral infection, have been documented. In this review, we will summarize the documented biological functions of IE1 and pp71 proteins, especially with regard to how they counteract cellular intrinsic defenses.展开更多
PP71 gene of the Human cytomegalor virur(HCMV AD-169) strain was amplified by PCR.After digestion by BamH I and Hind Ⅲ, the fragment was cloned into the high level expression vector pET28a.Recombinant plasmid pET28a-...PP71 gene of the Human cytomegalor virur(HCMV AD-169) strain was amplified by PCR.After digestion by BamH I and Hind Ⅲ, the fragment was cloned into the high level expression vector pET28a.Recombinant plasmid pET28a-PP71 was transformed into E.coli BL21(DE3) and lnduced by IPTG,high level protein was produced,and the target protein was 40% of all proteins.Purification recovery rate was high and up to 92.4% of the target protein before purification.which provides scientific basis on research of HCMV pathogenesis and diagnosis.展开更多
基金supported by a pilot grant from the Research Center for Minority Institutes (RCMI) program (2G12RR003050-24/8G12MD007579-27) (Q.T.)an American Cancer Society grant (RSG-090289-01MPC) (Q.T)+1 种基金NIH/NIAID SC1AI112785 (Q.T.)the Ponce Health Sciences University/RCMI Publications Office (G12 RR003050/8G12MD007579-27)
文摘Three crucial hurdles hinder studies on human cytomegalovirus(HCMV): strict species specificity, differences between in vivo and in vitro infection, and the complexity of gene regulation. Ever since the sequencing of the whole genome was first accomplished, functional studies on individual genes have been the mainstream in the CMV field. Gene regulation has therefore been elucidated in a more detailed fashion. However, viral gene regulation is largely controlled by both cellular and viral components. In other words, viral gene expression is determined by the virus–host interaction. Generally, cells respond to viral infection in a defensive pattern; at the same time, viruses try to counteract the cellular defense or else hide in the host(latency). Viruses evolve effective strategies against cellular defense in order to achieve replicative success. Whether or not they are successful, cellular defenses remain in the whole viral replication cycle: entry, immediate–early(IE) gene expression, early gene expression, DNA replication, late gene expression, and viral egress. Many viral strategies against cellular defense, and which occur in the immediate–early time of viral infection, have been documented. In this review, we will summarize the documented biological functions of IE1 and pp71 proteins, especially with regard to how they counteract cellular intrinsic defenses.
文摘PP71 gene of the Human cytomegalor virur(HCMV AD-169) strain was amplified by PCR.After digestion by BamH I and Hind Ⅲ, the fragment was cloned into the high level expression vector pET28a.Recombinant plasmid pET28a-PP71 was transformed into E.coli BL21(DE3) and lnduced by IPTG,high level protein was produced,and the target protein was 40% of all proteins.Purification recovery rate was high and up to 92.4% of the target protein before purification.which provides scientific basis on research of HCMV pathogenesis and diagnosis.
