To make up an engineered E.coli strain for expression of antigenic determinants of pp150 of human cytomegalovirus,the gene encoding the 420-752 amino acid peptides of pp150 was amplified from human cytomegalovirus AD1...To make up an engineered E.coli strain for expression of antigenic determinants of pp150 of human cytomegalovirus,the gene encoding the 420-752 amino acid peptides of pp150 was amplified from human cytomegalovirus AD169 by RT-PCR and cloned into Nde I/BamHⅠ sites in pET30a.The recombinant plasmid was transformed into E.coli BL21(DE3)and a foreign protein about 35 kD were detected by SDS-PAGE and Western blotting assay.The antiserum prepared by using prokaryotic expressed product as antigen turned to be against pp150 specifically and had low neutralization effect to HCMV.展开更多
文摘To make up an engineered E.coli strain for expression of antigenic determinants of pp150 of human cytomegalovirus,the gene encoding the 420-752 amino acid peptides of pp150 was amplified from human cytomegalovirus AD169 by RT-PCR and cloned into Nde I/BamHⅠ sites in pET30a.The recombinant plasmid was transformed into E.coli BL21(DE3)and a foreign protein about 35 kD were detected by SDS-PAGE and Western blotting assay.The antiserum prepared by using prokaryotic expressed product as antigen turned to be against pp150 specifically and had low neutralization effect to HCMV.
