Preliminary studies on emerging herbal ingredients have highlighted alternative pathways that inflammation and modulate perturbed immunity as valuable strategies for treating depression.Previous studies have shown tha...Preliminary studies on emerging herbal ingredients have highlighted alternative pathways that inflammation and modulate perturbed immunity as valuable strategies for treating depression.Previous studies have shown that 5-O-methylvisammioside,a bioactive compound derived from Saposhnikoviae Radix,possesses excellent anti-inflammatory and antioxidant biological functions,exhibits a neuroprotective effect.The purpose of this study was to explore the targets and signaling pathways of 5-O-methylvisammioside in the potential treatment of major depressive disorder using a combination of network pharmacology analysis and biological experiments.The network pharmacological analysis results indicated that the proto-oncogene tyrosine-protein kinase Src and the nuclear factor kappa B signaling pathway were highly correlated with the treatment of major depressive disorder with 5-O-methylvisammioside.Further experiments indicated that 5-O-methylvisammioside significantly improved lipopolysaccharide-induced depression-like behaviors in mice,ameliorated microglial polarization in the hippocampal CA1 and CA3 regions,and inhibited Src phosphorylation and nuclear factor kappa B pathway activation.The effects of 5-O-methylvisammioside were similar to those of the Src inhibitor PP2.When 5-O-methylvisammioside was administered with PP2,no effects were observed on lipopolysaccharide-induced depression-like behaviors in mice,nuclear factor kappa B pathway proteins,and microglial polarization.These findings indicate that 5-O-methylvisammioside may exert its antidepressant potential by inhibiting Src-mediated activation of the nuclear factor kappa B signaling pathway.Therefore,5-O-methylvisammioside might serve as a promising Chinese herbal medicine for the prevention and treatment of depression.展开更多
针对香菇育种过程中表型性状考察困难、人工测量费时费力的问题,提出一种适用于Jetson Orin Nano平台部署的香菇菌棒表型测量方法。使用手机和工业相机对3种不同数据来源的香菇菌棒进行图像采集,标注香菇菌落数据集并进行增强;比较了Bis...针对香菇育种过程中表型性状考察困难、人工测量费时费力的问题,提出一种适用于Jetson Orin Nano平台部署的香菇菌棒表型测量方法。使用手机和工业相机对3种不同数据来源的香菇菌棒进行图像采集,标注香菇菌落数据集并进行增强;比较了Bisenet、Stdcseg、U-net、Deeplabv3p、PP-liteseg 5个分割模型在Test-A、Test-B和Test-C测试集上的分割效果,结果表明PP-liteseg的普适性优于其他网络,PP-liteseg分割模型在3个测试集上的平均交并比超过97.53%,平均像素准确率高于99.49%,推理单幅图像耗时660 ms;为进一步平衡模型准确性和实时性,采用量化蒸馏方式对PP-liteseg模型进行压缩,并部署到Jetson Orin Nano平台上,压缩后的模型在Test-B测试集上的平均交并比和平均像素准确率分别为97.50%和99.51%,推理单幅图像耗时43.63 ms,比压缩前缩短近64%;采用PP-liteseg分割香菇菌棒图像,提取菌落表型,再根据菌落径向长度和轴向宽度得到菌丝生长长度,与人工测量值相比,菌丝生长长度平均绝对百分比误差、均方根误差和决定系数分别为1.874%、0.148 cm、0.918;采用该方法测量了6个菌株在连续4 d内的菌丝生长长度,结果表明,6个菌株的菌丝生长速度无论在单日还是在整个培养期内均有差异,其中49号和168号菌株差异最大。研究表明,本文方法适用于香菇菌棒表型测量,并能够以良好的准确性和实时性在Jetson Orin Nano平台上运行。展开更多
基金supported by a grant from Department of Science and Technology of Jilin Province,No.20230204039YY(to RC)the Construction Project for Antidepressant Screening Engineering Laboratory of Jilin Province,No.2023C010(to BL).
文摘Preliminary studies on emerging herbal ingredients have highlighted alternative pathways that inflammation and modulate perturbed immunity as valuable strategies for treating depression.Previous studies have shown that 5-O-methylvisammioside,a bioactive compound derived from Saposhnikoviae Radix,possesses excellent anti-inflammatory and antioxidant biological functions,exhibits a neuroprotective effect.The purpose of this study was to explore the targets and signaling pathways of 5-O-methylvisammioside in the potential treatment of major depressive disorder using a combination of network pharmacology analysis and biological experiments.The network pharmacological analysis results indicated that the proto-oncogene tyrosine-protein kinase Src and the nuclear factor kappa B signaling pathway were highly correlated with the treatment of major depressive disorder with 5-O-methylvisammioside.Further experiments indicated that 5-O-methylvisammioside significantly improved lipopolysaccharide-induced depression-like behaviors in mice,ameliorated microglial polarization in the hippocampal CA1 and CA3 regions,and inhibited Src phosphorylation and nuclear factor kappa B pathway activation.The effects of 5-O-methylvisammioside were similar to those of the Src inhibitor PP2.When 5-O-methylvisammioside was administered with PP2,no effects were observed on lipopolysaccharide-induced depression-like behaviors in mice,nuclear factor kappa B pathway proteins,and microglial polarization.These findings indicate that 5-O-methylvisammioside may exert its antidepressant potential by inhibiting Src-mediated activation of the nuclear factor kappa B signaling pathway.Therefore,5-O-methylvisammioside might serve as a promising Chinese herbal medicine for the prevention and treatment of depression.
文摘针对香菇育种过程中表型性状考察困难、人工测量费时费力的问题,提出一种适用于Jetson Orin Nano平台部署的香菇菌棒表型测量方法。使用手机和工业相机对3种不同数据来源的香菇菌棒进行图像采集,标注香菇菌落数据集并进行增强;比较了Bisenet、Stdcseg、U-net、Deeplabv3p、PP-liteseg 5个分割模型在Test-A、Test-B和Test-C测试集上的分割效果,结果表明PP-liteseg的普适性优于其他网络,PP-liteseg分割模型在3个测试集上的平均交并比超过97.53%,平均像素准确率高于99.49%,推理单幅图像耗时660 ms;为进一步平衡模型准确性和实时性,采用量化蒸馏方式对PP-liteseg模型进行压缩,并部署到Jetson Orin Nano平台上,压缩后的模型在Test-B测试集上的平均交并比和平均像素准确率分别为97.50%和99.51%,推理单幅图像耗时43.63 ms,比压缩前缩短近64%;采用PP-liteseg分割香菇菌棒图像,提取菌落表型,再根据菌落径向长度和轴向宽度得到菌丝生长长度,与人工测量值相比,菌丝生长长度平均绝对百分比误差、均方根误差和决定系数分别为1.874%、0.148 cm、0.918;采用该方法测量了6个菌株在连续4 d内的菌丝生长长度,结果表明,6个菌株的菌丝生长速度无论在单日还是在整个培养期内均有差异,其中49号和168号菌株差异最大。研究表明,本文方法适用于香菇菌棒表型测量,并能够以良好的准确性和实时性在Jetson Orin Nano平台上运行。
