Dear Editor,Small extracellular vesicles(sEVs)are membranous nanovesicles involved in intercellular,communication that carry distinct cellderived molecular cargo.1 We previously characterised sEVs from human non-malig...Dear Editor,Small extracellular vesicles(sEVs)are membranous nanovesicles involved in intercellular,communication that carry distinct cellderived molecular cargo.1 We previously characterised sEVs from human non-malignant pancreatic duct cells(HPDE,hTERT-HPNE)and from PDAC cells(AsPC-1,BxPC-3 and MIA PaCa-2)2 and identified protein cargo-specific to cancer-associated sEVs?Among the proteins uniquely expressed in cancer sEVs but not in those from non-malignant cells,we focused on SLC5A3,also known as SMIT1(sodium-coupled Myo-inositol transporter-1).展开更多
Objective To investigate the status of evoked potentials in obsessive-compulsive disorder(OCD). Methods Evoked potentials P300, auditory brainstem response (ABR) and visual evoked potential ( VEP) were recorded from 3...Objective To investigate the status of evoked potentials in obsessive-compulsive disorder(OCD). Methods Evoked potentials P300, auditory brainstem response (ABR) and visual evoked potential ( VEP) were recorded from 35 OCD patients and 28 normal controls (NC) with a Nicolet Spirit Instrument. 23 of the OCD patients were followed up after 5 months with the same markers. Results Compared with NC, OCD patients showed decreased P3 of P300 amplitude (OCD group 3. 5 ±1. 6μv vs. NC group 5.9 ±2. 1μv, P <0. 01), delayed V latency (6.4±0. 4ms vs. 5. 5 ±0. 3ms, P <0. 01) and increased V amplitude(0. 35±0. 1μv vs. 0. 16 ±0.09μv, P <0. 05) of ABR and delayed P2 of VEP latency (199±39ms vs. 183±28ms, P <0. 05). The follow-up measures of evoked potentials suggested that decreased P3 of P300 amplitude and delayed P2 of VEP latency might be state markers of OCD, while decreased V amplitude and delayed V of ABR latency might be trait markers of OCD. Conclusion The changes of P300 and VEP are related to clinical status of OCD patients, while the association between ABR and OCD symptoms need to be further investigated.展开更多
Pancreatic cancer has a tough early diagnosis and awful prognosis among all human cancers.Tumor heterogeneity can hinder the exploration of tumor progression because it can induce various discrepancies in tumor growth...Pancreatic cancer has a tough early diagnosis and awful prognosis among all human cancers.Tumor heterogeneity can hinder the exploration of tumor progression because it can induce various discrepancies in tumor growth rate,invasion and metastasis ability,and drug susceptibility.Here,we proposed 3D-hetero-type multicellular spheroids(3D-HMS)and investigated the spatial distribution of lipids during the spatiotemporal progression of pancreatic cancer.The results showed that the malignant degree of 3D-HMS increased during the tumor progression,including an increase in the positively expressed micro-region of CK,the absence of FN positive expression,and significant spatial heterogeneity of cell states,which were consistent with the growth state and internal structure of solid tumor tissues in pancreatic cancer.Additionally,spatial distributions of lipids in 3D-HMS exhibited spatiotemporal heterogeneity.MSI-based 3D-HMS construction and lipid analysis will provide a new insight into the occurrence of metastasis,spatiotemporal progression,as well as early cancer detection.展开更多
基金supported by National Health and Medical Research Council(NHMRC,MRF2015523,APP1141946)Helen Amelia Hains Fellowship(D.W.G.)and Department of Defense(PR230065)supported by the Snow Medical Research Fellowship,NHMRC Investigator 2027300 and Philip Hemstritch Fellowship.
文摘Dear Editor,Small extracellular vesicles(sEVs)are membranous nanovesicles involved in intercellular,communication that carry distinct cellderived molecular cargo.1 We previously characterised sEVs from human non-malignant pancreatic duct cells(HPDE,hTERT-HPNE)and from PDAC cells(AsPC-1,BxPC-3 and MIA PaCa-2)2 and identified protein cargo-specific to cancer-associated sEVs?Among the proteins uniquely expressed in cancer sEVs but not in those from non-malignant cells,we focused on SLC5A3,also known as SMIT1(sodium-coupled Myo-inositol transporter-1).
基金Supported by the Fund of ' 100 Distinguished Investigator' of Shanghai Sanitary Authorities(97BR030)Fund of Clinical Medicine Center of Shanghai Mental Disorder(ZX02A14).
文摘Objective To investigate the status of evoked potentials in obsessive-compulsive disorder(OCD). Methods Evoked potentials P300, auditory brainstem response (ABR) and visual evoked potential ( VEP) were recorded from 35 OCD patients and 28 normal controls (NC) with a Nicolet Spirit Instrument. 23 of the OCD patients were followed up after 5 months with the same markers. Results Compared with NC, OCD patients showed decreased P3 of P300 amplitude (OCD group 3. 5 ±1. 6μv vs. NC group 5.9 ±2. 1μv, P <0. 01), delayed V latency (6.4±0. 4ms vs. 5. 5 ±0. 3ms, P <0. 01) and increased V amplitude(0. 35±0. 1μv vs. 0. 16 ±0.09μv, P <0. 05) of ABR and delayed P2 of VEP latency (199±39ms vs. 183±28ms, P <0. 05). The follow-up measures of evoked potentials suggested that decreased P3 of P300 amplitude and delayed P2 of VEP latency might be state markers of OCD, while decreased V amplitude and delayed V of ABR latency might be trait markers of OCD. Conclusion The changes of P300 and VEP are related to clinical status of OCD patients, while the association between ABR and OCD symptoms need to be further investigated.
基金supported by the National Natural Science Foundation of China(22176195,82127801)the Guangdong Province Zhu Jiang Talents Plan(2021QN02Y028)+4 种基金the National Key R&D Program of China(2022YFF0705003)the Key Program of Fundamental Research in Shenzhen(JCYJ20210324115811031)the National Key R&D Program of China(2022YFF0705003)the Shen-zhen Key Laboratory of Precision Diagnosis and Treatment of Depression(ZDSYS20220606100606014)the Guangdong Science and Technology Department(2021B1212030004)。
文摘Pancreatic cancer has a tough early diagnosis and awful prognosis among all human cancers.Tumor heterogeneity can hinder the exploration of tumor progression because it can induce various discrepancies in tumor growth rate,invasion and metastasis ability,and drug susceptibility.Here,we proposed 3D-hetero-type multicellular spheroids(3D-HMS)and investigated the spatial distribution of lipids during the spatiotemporal progression of pancreatic cancer.The results showed that the malignant degree of 3D-HMS increased during the tumor progression,including an increase in the positively expressed micro-region of CK,the absence of FN positive expression,and significant spatial heterogeneity of cell states,which were consistent with the growth state and internal structure of solid tumor tissues in pancreatic cancer.Additionally,spatial distributions of lipids in 3D-HMS exhibited spatiotemporal heterogeneity.MSI-based 3D-HMS construction and lipid analysis will provide a new insight into the occurrence of metastasis,spatiotemporal progression,as well as early cancer detection.
