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牛副流感病毒3型NP蛋白在昆虫杆状病毒系统中的表达
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作者 项钰 郭衍冰 +7 位作者 孙兴忠 郝良玉 张天宇 张艺馨 姜乐凡 周子博 王楠 曹利利 《畜牧与兽医》 北大核心 2026年第1期101-106,共6页
为表达牛副流感病毒3型(BPIV3)核衣壳蛋白(NP),利用昆虫表达系统构建pFastBAC-NP重组质粒,经转座获得重组杆粒Bacmid-NP,转染昆虫细胞(Sf9)后获得重组杆状病毒。通过Bacmid-NP感染Sf9细胞表达NP蛋白,利用高浓度咪唑洗脱纯化NP蛋白,利用... 为表达牛副流感病毒3型(BPIV3)核衣壳蛋白(NP),利用昆虫表达系统构建pFastBAC-NP重组质粒,经转座获得重组杆粒Bacmid-NP,转染昆虫细胞(Sf9)后获得重组杆状病毒。通过Bacmid-NP感染Sf9细胞表达NP蛋白,利用高浓度咪唑洗脱纯化NP蛋白,利用半数组织培养感染剂量(TCID_(50))测定病毒滴度,SDS-PAGE和Western blot分析NP蛋白的纯化效果,BCA测定蛋白浓度,并将蛋白免疫小鼠,检测免疫后抗体水平。结果:构建的Bacmid-NP采用pUC/M13通用引物验证,证实NP基因成功克隆至Bacmid杆粒;P3代重组病毒的TCID_(50)为10^(-6.86)/0.1 mL;Bacmid-NP传代后Western blot检测,可在67 ku出现特异性条带;通过SDS-PAGE对感染Bacmid-NP的Sf9细胞进行检测,结果表明上清液与沉淀均可表达;Western blot鉴定结果表明67 ku左右有特异性反应条带,说明NP蛋白可以分泌表达;采用镍柱纯化,SDSPAGE鉴定纯化效果,并将纯化蛋白与BPIV3阳性血清进行Western blot试验,结果显示纯化蛋白为单条带,可以与阳性血清特异性结合,蛋白浓度为0.596 mg/mL,免疫小鼠后血清效价可达1∶64000。综上,本试验利用昆虫表达系统成功表达了NP蛋白,为进一步建立检测方法和疫苗研制提供了技术基础。 展开更多
关键词 牛副流感病毒3型 杆状病毒表达系统 np蛋白
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从能否去掉“的”再探“NP的VP”结构
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作者 闫晓凤 《现代语言学》 2026年第1期755-761,共7页
从“这本书的出版”引发的关于“NP的VP”的结构问题学术界已展开众多讨论。不同于大多聚焦于词类及其转换的研究,本文从实际语料出发,将重点讨论能否去掉“的”,分析这一特殊用法的表现特征及形成的原因。本文发现,在去掉“的”不影响... 从“这本书的出版”引发的关于“NP的VP”的结构问题学术界已展开众多讨论。不同于大多聚焦于词类及其转换的研究,本文从实际语料出发,将重点讨论能否去掉“的”,分析这一特殊用法的表现特征及形成的原因。本文发现,在去掉“的”不影响语用的句子中,VP主要由一般或具体的动词构成,并与前面的名词构成主动关系;在去掉“的”后不成立或难以成立的句子中,动词多为抽象的动词,转化为名词义的能力较强,对“的”依赖性较强,此时动词与“的”前面的名词多构成被动关系。后者中的动词很可能是兼类词,其名词义与动词义都被广泛应用。上述现象表明,“NP的VP”结构中“的”的可省略性,与动词的名物化倾向及其兼类属性密切相关。 展开更多
关键词 名物化 np的VP 动词 兼类
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Revealing the dynamic responses of Pb under shock loading based on DFT-accuracy machine learning potential
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作者 Enze Hou Xiaoyang Wang Han Wang 《Chinese Physics B》 2026年第1期57-64,共8页
Lead(Pb)is a typical low-melting-point ductile metal and serves as an important model material in the study of dynamic responses.Under shock-wave loading,its dynamic mechanical behavior comprises two key phenomena:pla... Lead(Pb)is a typical low-melting-point ductile metal and serves as an important model material in the study of dynamic responses.Under shock-wave loading,its dynamic mechanical behavior comprises two key phenomena:plastic deformation and shock-induced phase transitions.The underlying mechanisms of these processes are still poorly understood.Revealing these mechanisms remains challenging for experimental approaches.Non-equilibrium molecular dynamics(NEMD)simulations are an alternative theoretical tool for studying dynamic responses,as they capture atomic-scale mechanisms such as defect evolution and deformation pathways.However,due to the limited accuracy of empirical interatomic potentials,the reliability of previous NEMD studies has been questioned.Using our newly developed machine learning potential for Pb-Sn alloys,we revisited the microstructural evolution in response to shock loading under various shock orientations.The results reveal that shock loading along the[001]orientation of Pb exhibits a fast,reversible,and massive phase transition and stacking-fault evolution.The behavior of Pb differs from previous studies by the absence of twinning during plastic deformation.Loading along the[011]orientation leads to slow,irreversible plastic deformation,and a localized FCC-BCC phase transition in the Pitsch orientation relationship.This study provides crucial theoretical insights into the dynamic mechanical response of Pb,offering a theoretical input for understanding the microstructure-performance relationship under extreme conditions. 展开更多
关键词 interatomic potentials molecular dynamics shock impacts machine learning
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Sequential phase transformations in Ta_(0.4)Ti_(2)Zr alloy via tensile molecular dynamics simulations with deep potential
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作者 Hongyang Liu Rong Chen +3 位作者 Bo Chen Jingzhi He Dongdong Kang Jiayu Dai 《Chinese Physics B》 2026年第1期46-55,共10页
Understanding the complex deformation mechanisms of non-equimolar multi-principal element alloys(MPEAs)requires high-fidelity atomic-scale simulations.This study develops a deep potential(DP)model to enable molecular ... Understanding the complex deformation mechanisms of non-equimolar multi-principal element alloys(MPEAs)requires high-fidelity atomic-scale simulations.This study develops a deep potential(DP)model to enable molecular dynamics simulations of the Ta_(0.4)Ti_(2)Zr(Ta_(0.4))alloy.Monte Carlo simulations using this potential reveal Ta atom precipitation in the Ta_(0.4)alloy.Under uniaxial tensile loading along the[100]direction in the NPT ensemble,the alloy undergoes a remarkable sequence of phase transformations:an initial body-centered cubic(BCC_(1))to face-centered cubic(FCC)transformation,followed by a reverse transformation from FCC to a distinct BCC phase(BCC_(2)),and finally a BCC_(2) to hexagonal close-packed(HCP)transformation.Critically,the reverse FCC to BCC_(2) transformation induces significant volume contraction.We demonstrate that the inversely transformed BCC_(2) phase primarily accommodates compressive stress.Concurrently,the reorientation of BCC_(2) crystals contributes substantially to the observed high strain hardening.These simulations provide atomic-scale insights into the dynamic structural evolution,sequential phase transformations,and stress partitioning during deformation of the Ta_(0.4)alloy.The developed DP model and the revealed mechanisms offer fundamental theoretical guidance for accelerating the design of high-performance MPEAs. 展开更多
关键词 multi-principal element alloys machine-learning potential phase transformation stress partitioning
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Structures and dynamics of helium in liquid lithium:A study by deep potential molecular dynamics
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作者 Xinyu Zhu Jianchuan Liu +5 位作者 Tao Chen Xinyue Xie Jin Wang Yi Xie Chenxu Wang Mohan Chen 《Chinese Physics B》 2026年第1期25-33,共9页
Current experimental techniques still face challenges in clarifying the structural and dynamic properties of helium(He)in liquid lithium(Li).A critical example of this technical hurdle is the formation of He bubbles,w... Current experimental techniques still face challenges in clarifying the structural and dynamic properties of helium(He)in liquid lithium(Li).A critical example of this technical hurdle is the formation of He bubbles,which significantly affects the transport of He within liquid Li—a vital aspect when considering liquid Li as a plasma-facing material in nuclear fusion reactors.We develop a machine-learning-based deep potential(DP)with ab initio accuracy for the Li-He system and perform molecular dynamics simulations at temperatures ranging from 470 K to 1270 K with a wide range of He concentrations.We observe that He atoms exhibit a tendency to aggregate and form clusters and bubbles in liquid Li.Notably,He clusters exhibit a significant increase in size at elevated temperatures and high concentrations of He,accompanied by the phase separation of Li and He atoms.We also observe an anomalous non-linear relationship between the diffusion coefficient of He and temperature,which is attributed to the larger cluster size at higher temperatures.Our study provides a deeper understanding of the behavior of He in liquid Li and further supports the potential application of liquid Li under extreme conditions. 展开更多
关键词 MD simulation machine-learning-based deep potential plasma-facing material He in liquid Li
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Oxidative potential of PM_(2.5) in Guangzhou,Southern China:Source apportionment and association with airborne bacteria
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作者 Yuxin Huang Senchao Lai +7 位作者 Baoling Liang Jinpu Zhang Chenglei Pei Dachi Hong Xiaoluan Lin Zhaokang Ruan Leitao Sun Yingyi Zhang 《Journal of Environmental Sciences》 2026年第1期64-72,共9页
Oxidative potential(OP)can be used as an indicator of the health risks of particulate matter in the air.To study the variation and sources of OP,we conducted an observation of PM_(2.5) in a megacity in southern China ... Oxidative potential(OP)can be used as an indicator of the health risks of particulate matter in the air.To study the variation and sources of OP,we conducted an observation of PM_(2.5) in a megacity in southern China in winter and spring of 2021.The results show that the average concentration of PM_(2.5) decreased by 47%from winter to spring,while volume-normalized and mass-normalized OP(i.e.,OP_(v) and OP_(m))increased by 6%and 69%,respectively.It suggests that the decline of PM_(2.5) may not necessarily decrease the health risks and the intrinsic toxicity of PM_(2.5).Variations of OP_(v) and OP_(m) among different periods were related to the different source contributions and environmental conditions.The positive matrix factorization model was used to identify the major sources of OP_(v).OP_(v) was mainly contributed by biomass burning/industrial emissions(29%),soil/road dust(20%),secondary sulfate(14%),and coal combustion(13%)in winter.Different major sources were resolved to be secondary sulfate(36%),biological sources(21%),and marine vessels(20%)in spring,presenting the substantial contribution of biological sources.The analysis shows strong associations between OP_(v) and both live and dead bacteria,further confirming the important contribution of bioaerosols to the enhancement of OP.This study highlights the importance of understanding OP in ambient PM_(2.5) in terms of public health impact and provides a new insight into the biological contribution to OP. 展开更多
关键词 Oxidative potential PM_(2.5) Source apportionment Positive matrix factorization Airborne bacteria
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Transmission pathways and potential source regions for atmospheric fine particulate matter and ozone in Urumqi
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作者 Aifang Gao Wanting Sun +4 位作者 Xi You Chenglong Liao Baojun Zhang Mengyue Wang Hongliang Zhang 《Journal of Environmental Sciences》 2026年第1期683-693,共11页
To understand the transmission paths and potential source areas of fine particulate matter(PM_(2.5))and ozone(O_(3))in Urumqi,using the monitoring data from 2020 to 2022,the pollution characteristics and the transmiss... To understand the transmission paths and potential source areas of fine particulate matter(PM_(2.5))and ozone(O_(3))in Urumqi,using the monitoring data from 2020 to 2022,the pollution characteristics and the transmission paths of PM_(2.5) and O_(3) were studied.Based on the MeteoInfo software,the potential source areas and concentration contributions via the weighted potential source contribution function(WPSCF)and the weighted concentration weighted trajectory(WCWT)were analyzed.Besides,trajectory distribution at different starting heights were compared.The results of the backward trajectory(500 m)showed that the PM_(2.5) and O_(3) clustering trajectories were mainly derived from the northwest and passed through Yining/Ili and Tacheng.The air flow proportion of PM_(2.5) pollution was 44.83%in winter.With the highest pollution concentration(119.2μg/m^(3)),the pollution airflow proportion of O_(3) was 30.52%in summer.According to an analysis of the pressure profile,the atmospheric pressure was below 850 hPa in winter,indicating that the near-surface air mass had a substantial impact on PM_(2.5) concentrations,whereas in summer the pressure for O_(3) rose above 750 hP1,leading to higher pollutant concentrations.The WPSCF/WCWT results of PM_(2.5) demonstrated that the largest potential areas were identified in winter and were mainly distributed in Bozhou,Kuitun,and Shihezi(west of Urumqi,cultivated land/grassland),while the largest potential areas of O_(3) were distributed in Changji(east of Urumqi,barren land)and Turpan(southeast of Urumqi,grassland)in summer.The study indicates that the government should implement stricter measures to control regional transmission and air pollution. 展开更多
关键词 URUMQI Air pollution PM_(2.5)and O_(3) potential sourceanalysis Backward trajectory
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Retraction: Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+ Ovarian Cancer Stem Cells
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作者 Oncology Research Editorial Office 《Oncology Research》 2026年第1期620-620,共1页
The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Re... The published article titled“Truncated Bid Overexpression Induced by Recombinant Adenovirus Cre/LoxP System Suppresses the Tumorigenic Potential of CD133+Ovarian Cancer Stem Cells”has been retracted from Oncology Research,Vol.25,No.4,2017,pp.595–603. 展开更多
关键词 recombinant adenovirus Cre Loxp system CD ovarian cancer stem cells recombinant adenovirus truncated Bid overexpression bid overexpression tumorigenic potential
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Big Screen Boom:The robust growth of China’s film market points to a strong postpandemic recovery and reflects its immense potential
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作者 LU JIAJUN 《ChinAfrica》 2026年第2期54-55,共2页
The year 2025 marks the 120th anniversary of the birth of Chinese filmmaking.From the first film Dingjun Mountain released in 1905,which captured scenes from Peking opera,to the present day where artificial intelligen... The year 2025 marks the 120th anniversary of the birth of Chinese filmmaking.From the first film Dingjun Mountain released in 1905,which captured scenes from Peking opera,to the present day where artificial intelligence(AI)is utilised in film production,the Chinese film industry has been developing for over a century.Data from the China Film Administration shows that China’s 2025 box o"ce revenue topped 51.8 billion yuan($7.4 billion),realising a year-on-year increase of nearly 22 percent. 展开更多
关键词 postpandemic recovery robust growth box office revenue chinese film industry artificial intelligence ai th anniversary film dingjun mountain immense potential
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NPP vulnerability of the potential vegetation of China to climate change in the past and future 被引量:28
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作者 YUAN Quanzhi WU Shaohong +3 位作者 DAI Erfu ZHAO Dongsheng REN Ping ZHANG Xueru 《Journal of Geographical Sciences》 SCIE CSCD 2017年第2期131-142,共12页
Using the Integrated Biosphere Simulator, a dynamic vegetation model, this study initially simulated the net primary productivity(NPP) dynamics of China's potential vegetation in the past 55 years(1961–2015) and... Using the Integrated Biosphere Simulator, a dynamic vegetation model, this study initially simulated the net primary productivity(NPP) dynamics of China's potential vegetation in the past 55 years(1961–2015) and in the future 35 years(2016–2050). Then, taking the NPP of the potential vegetation in average climate conditions during 1986–2005 as the basis for evaluation, this study examined whether the potential vegetation adapts to climate change or not. Meanwhile, the degree of inadaptability was evaluated. Finally, the NPP vulnerability of the potential vegetation was evaluated by synthesizing the frequency and degrees of inadaptability to climate change. In the past 55 years, the NPP of desert ecosystems in the south of the Tianshan Mountains and grassland ecosystems in the north of China and in western Tibetan Plateau was prone to the effect of climate change. The NPP of most forest ecosystems was not prone to the influence of climate change. The low NPP vulnerability to climate change of the evergreen broad-leaved and coniferous forests was observed. Furthermore, the NPP of the desert ecosystems in the north of the Tianshan Mountains and grassland ecosystems in the central and eastern Tibetan Plateau also had low vulnerability to climate change. In the next 35 years, the NPP vulnerability to climate change would reduce the forest–steppe in the Songliao Plain, the deciduous broad-leaved forests in the warm temperate zone, and the alpine steppe in the central and western Tibetan Plateau. The NPP vulnerability would significantly increase of the temperate desert in the Junggar Basin and the alpine desert in the Kunlun Mountains. The NPP vulnerability of the subtropical evergreen broad-leaved forests would also increase. The area of the regions with increased vulnerability would account for 27.5% of China. 展开更多
关键词 climate change VULNERABILITY potential vegetation net primary productivity IBIS China
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A novel SVPWM strategy considering neutral-point potential balancing for three-level NPC inverter 被引量:2
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作者 陈国呈 宋文祥 +1 位作者 武慧 孙承波 《Journal of Shanghai University(English Edition)》 CAS 2006年第1期53-58,共6页
This paper proposes a novel SVPWM (space vector pulse width modulation) strategy for the three-level neutral-point-clamped voltage source inverter, based on the particular disposition of all the redundant voltage ve... This paper proposes a novel SVPWM (space vector pulse width modulation) strategy for the three-level neutral-point-clamped voltage source inverter, based on the particular disposition of all the redundant voltage vectors. The new modulation approach shows superior performance for harmonic voltage and balancing control of neutral-point potential compared to the popular eight-stage centered SVPWM. It realizes suppression of inverter neutral-point potential variation by accurately modifying redundant factor of small vectors pairs, only requiring information of DC-link capacitor voltages and three-phase load currents. This is convenient to apply and is compatible of digital computer realization. Feasibility of the proposed control approach is verified by simulation and experimental results. 展开更多
关键词 neutral-point-clamped inverter neutral-point potential control SVPWM distribution factor.
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Effect of Potential Energy Stored in Reactor Facility Coolant on NPP Safety and Economic Parameters 被引量:1
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作者 G. I. Toshinsky O. G. Komlev +1 位作者 I. V. Tormyshev V. V. Petrochenko 《World Journal of Nuclear Science and Technology》 2013年第2期59-64,共6页
Potential (non-nuclear) energy stored in reactor facility coolant is a crucial factor determining the NPP safety/hazard characteristics as it is inherent property of the material and cannot be changed. Enhancing safet... Potential (non-nuclear) energy stored in reactor facility coolant is a crucial factor determining the NPP safety/hazard characteristics as it is inherent property of the material and cannot be changed. Enhancing safety of the NPP with traditional type reactor facilities, in which potential energy is stored in large quantities, requires buildup of the number of safety systems and in-depth defense barriers, which reduce the probability of severe accidents (but do not exclude the opportunity of their realization) and seriousness of their consequences. Keeping the risk of radioactivity release for different type reactor facilities at a same level of social acceptability, the number of safety systems and in-depth defense barriers, which determine essentially the NPP economical parameters, can be reduced with diminishing the potential energy stored in the reactor facility. To analyze the effect of potential energy on reactor facility safety/hazard, a diagram of reactor facility hazard has been proposed. It presents a probability of radioactivity release as a function of radioactivity release values for reactor facilities with identical radiation potential, which differ by values of potential energy stored in coolant. It is proposed to account NPP safety/hazard effect on economics by adding a certain interest on the electricity cost for making payments in a special insurance fund assigned to compensate the expenses for elimination of consequences of a possible accident. 展开更多
关键词 potential Energy npP SAFETY COOLANT
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Ionization Potentials and Quantum Defects of 1s^2np^2p Rydberg States of Lithium Atom 被引量:1
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作者 CHEN Chao 《Communications in Theoretical Physics》 SCIE CAS CSCD 2008年第9期733-737,共5页
Abstract In this work, ionization potentials and quantum effects of ls^2 np^2 P Rydberg states of lithium are calculated based on the calibrated quantum defect function. Energy levels and quantum defects for ls^2np^2P... Abstract In this work, ionization potentials and quantum effects of ls^2 np^2 P Rydberg states of lithium are calculated based on the calibrated quantum defect function. Energy levels and quantum defects for ls^2np^2P bound states and their adjacent continuum states are calculated with the R-matrix theory, and then the quantum defect function of the ls^2np (n ≥ 7) channel is obtained, which varies smoothly with the energy based on the quantum defect theory. The accurate quantum defect of the ls^2 7p^2P state derived from the experimental data is used to calibrate the original quantum defect function. The new function is used to calculate ionization potentials and quantum effects of ls^2np ^2P (n ≥ 7) Rydberg states. Present calculations are in agreement with recent experimental data in whole. 展开更多
关键词 ionization potential quantum effect R-matrix theory
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环境水样中^(237)Np和^(241)Am分析方法的研究进展
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作者 文雯 娄海林 +3 位作者 文富平 王绍林 丛日俐 李玉芹 《核电子学与探测技术》 北大核心 2025年第10期1569-1584,共16页
环境样品中放射性核素的分析测量是辐射防护和辐射监测的关键过程,对评估放射性核素的环境影响和放射性风险具有重要意义。本文综述了环境水样中^(237)Np和^(241)Am分析方法的研究现状,在目前环境中^(237)Np和^(241)Am的放射性水平以及N... 环境样品中放射性核素的分析测量是辐射防护和辐射监测的关键过程,对评估放射性核素的环境影响和放射性风险具有重要意义。本文综述了环境水样中^(237)Np和^(241)Am分析方法的研究现状,在目前环境中^(237)Np和^(241)Am的放射性水平以及Np和Am化学性质的基础上,对水样预处理、分离纯化和测量三个方面进行了详细综述,总结分析了目前环境水样^(237)Np和^(241)Am分析方法的特点和难点,并展望了未来环境水样中^(237)Np和^(241)Am分析技术的发展方向。 展开更多
关键词 水样 ^(237)np ^(241)Am 分析 测量
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基于矢量分类的NPC型三电平双三相PMSM的改进模型预测电压控制 被引量:4
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作者 袁庆庆 步凡 +1 位作者 谢晓彤 夏鲲 《中国电机工程学报》 北大核心 2025年第2期758-768,I0030,共12页
多电平多相电机驱动系统存在电压矢量数目多、冗余复杂及多目标非线性约束等问题。以二极管钳位型三电平六相逆变器驱动双三相永磁同步电机为研究对象,以电机谐波电流抑制和逆变器直流侧中点电位平衡控制为目标,提出一种基于电压矢量三... 多电平多相电机驱动系统存在电压矢量数目多、冗余复杂及多目标非线性约束等问题。以二极管钳位型三电平六相逆变器驱动双三相永磁同步电机为研究对象,以电机谐波电流抑制和逆变器直流侧中点电位平衡控制为目标,提出一种基于电压矢量三角区域分类的改进模型预测电压控制策略。首先,以基波子平面大投影幅值为原则,对729个电压矢量进行初筛;接着,基于三角区域分类方法对筛选后的电压矢量进行分类,并对期望电压进行所属三角区域定位、确定候选矢量。设计满足电机定子电流谐波抑制及逆变器中点电位平衡控制的目标函数;根据电机数学模型和逆变器特征研究多目标控制权重因子的理论设计方法。不同工况下的有效性及对比实验表明,所研究的控制策略不仅能改善多电平多相电机驱动系统的多目标控制性能,还能有效降低控制器计算负担。 展开更多
关键词 二极管钳位型三电平六相逆变器 双三相永磁同步电机 矢量分类 谐波电流 中点电位 模型预测电压控制 权重因子
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基于NP语言的证据加密研究综述
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作者 王玉珠 张明武 《密码学报(中英文)》 北大核心 2025年第2期247-264,共18页
基于NP语言的证据加密是一种无需密钥生成阶段的新型加密方案,解密者拥有某NP问题实例对应的证据而不是密钥,这意味着接收者不需要事先指定,仅有拥有解密能力者(证据)才能解密.由于双方在通信之前不需要交换密钥,证据加密有很多有趣的... 基于NP语言的证据加密是一种无需密钥生成阶段的新型加密方案,解密者拥有某NP问题实例对应的证据而不是密钥,这意味着接收者不需要事先指定,仅有拥有解密能力者(证据)才能解密.由于双方在通信之前不需要交换密钥,证据加密有很多有趣的应用场景.另一方面,证据加密不仅是独立的加密原语,还可作为基础部件用于构造其他强大的密码学方案.目前,证据加密已受到研究人员的广泛重视,其研究方向主要分为两个分支.其一是通用证据加密,这类方案能支持所有NP问题,但大多依赖于强假设条件.其二是仅支持特定NP语言的证据加密,该分支着重基于经过深入研究的密码学假设,致力于实现实用性强的构造方案.本文对证据加密的安全模型、方案设计等作综述性研究和比较分析,探讨了经典的证据加密方案,归纳了证据加密的不同安全框架,剖析了证据加密的典型变体,同时对证据加密在构建其他密码原语方面的应用进行比较分析.最后结合证据加密相关的类似原语对今后的研究方向进行展望. 展开更多
关键词 证据加密 np语言 np完全语言 多线性映射 不可区分性混淆
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“NP的VP”式的DP结构重议
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作者 曹道根 潘海华 《语言科学》 北大核心 2025年第4期362-375,共14页
文章重新论证"NP的VP"式(如"这本书的出版")具有"内动外名"的句法构造。主要观点如下:1)只要承认其中"NP的"是领有成分,就必须承认此式有(零形)D功能投射,即此式有外层名词性句法构造。2)此式... 文章重新论证"NP的VP"式(如"这本书的出版")具有"内动外名"的句法构造。主要观点如下:1)只要承认其中"NP的"是领有成分,就必须承认此式有(零形)D功能投射,即此式有外层名词性句法构造。2)此式内层谓词性结构通常为词汇层VP或题元层vP/VoiceP,但名物化层级也存在向形态层最为有限的功能扩展。3)按所述物化事件是否涉及可数性特征核验或是否有数量描述,此式外层名词性架构区分为两类,差别在于DP内是否包含量词性轻名投射;量词性轻名词同时在语义和句法层面实现名物化。4)DP不含轻名投射的情形下(即[DP-VP]型式),领有名词移位生成;反之(即[DP-nP-VP]型式),领有名词基础生成于[Spec,DP],并成为VP中空主语或空宾语Pro的控制成分。5)"的"在此式中只是领格标记,既不构成此式作为名物化结构的结构中心,也不构成"NP的"这一领有短语的结构中心;"的"在此式中没有发生功能投射。 展开更多
关键词 名物化 np的VP DP结构
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与新城疫病毒NP蛋白互作宿主细胞蛋白的筛选和鉴定
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作者 韩银 谢紫葳 +2 位作者 严舒可 徐飞 陈瑞爱 《畜牧兽医学报》 北大核心 2025年第8期3942-3957,共16页
新城疫是由新城疫病毒(Newcastle disease virus,NDV)强毒株引起的一种急性接触性传染病,临床上常导致呼吸系统和消化系统的临床症状,NDV的广泛流行给家禽养殖业造成了巨大经济损失。NP(nucleocapsid)是NDV的一种核衣壳蛋白,在病毒复制... 新城疫是由新城疫病毒(Newcastle disease virus,NDV)强毒株引起的一种急性接触性传染病,临床上常导致呼吸系统和消化系统的临床症状,NDV的广泛流行给家禽养殖业造成了巨大经济损失。NP(nucleocapsid)是NDV的一种核衣壳蛋白,在病毒复制、介导免疫应答和引发细胞自噬方面具有重要作用,其是否存在其他相互作用的宿主蛋白尚不清楚。为了筛选与NDV NP互作的宿主蛋白,初步探索互作宿主蛋白对NDV复制的影响,为NDV抗病药物新靶点的筛选提供理论基础。利用真核表达载体pXJ40成功构建了NDV NP的真核表达质粒,通过免疫共沉淀、质谱分析、GST-pull down以及激光共聚焦等技术筛选出能与NP相互作用的宿主蛋白,进一步在DF-1细胞中通过敲低、过表达等方法探究互作宿主蛋白对NDV复制的影响。质谱结果筛选到与NP发生相互作用的潜在蛋白211个,对差异蛋白进行富集分析揭示了其可能发挥的生物学功能和参与的生物学过程,进一步验证了热休克蛋白家族成员70(HSP70)与NP相互作用,并且这种互作是由HSP70的SBD结构域介导的;NDV感染能下调宿主细胞内HSP70的表达;过表达HSP70能在蛋白和转录水平显著抑制病毒复制;敲低内源性HSP70和HSP70抑制剂均能显著促进NDV复制。本研究筛选出NDV NP的互作宿主蛋白HSP70,并且验证了过表达HSP70能显著抑制NDV复制,作为NDV感染的负调控因子,为设计以HSP70为靶点的抗病毒药物提供了新思路。 展开更多
关键词 新城疫病毒 np HSP70蛋白 免疫共沉淀技术
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CHARACTERISTICS OF ACTIONPOTENTIAL AND THEIR UNDERLYING OUTWARD CURRENTS IN MAMMALIAN TASTE RECEPTOR CELLS
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作者 陈玉社 《Journal of Pharmaceutical Analysis》 CAS 1995年第2期189-189,共1页
Many rat taste receptor cells conduct action potentials(APs).APs had a mean threshold of -35 mV(n=95 cells)and a spike height of 52mV above threshold in current clamp(hold= -80mV).Aps could be classified into two sign... Many rat taste receptor cells conduct action potentials(APs).APs had a mean threshold of -35 mV(n=95 cells)and a spike height of 52mV above threshold in current clamp(hold= -80mV).Aps could be classified into two significantly different (P<0.001) groups-fast,with short half-time durations and large outward currents (mean1.3 ms and 2.7nA),and slow,with long duration and small outward currents(mean9.2ms and 0. 29nA).AP upstrokes were conducted by TTX-sensitive sodium currents whereas the downstroke by TEA-blockable outward currents. Voltage dependent analysis of outward current separated transient and sustained components.The transient component was specifically blocked by 4-AP(1mmol/L).A calcium-dependent outward component was also revealed modulating voltage and external calcium concentration.The fast recovery phase of the AP appears related the sustained outward current whereas the after hyperpolarization(AHP) was blocked by 4AP suggesting a significant contribution of the transient component.Forskolin (FSK),which elevates cAMP,reversibly blocked the majority of the sustained current without influencing the transient. FSK greatly exaggerated the AHP without changing the spike height or duration. These data suggest that several components of the outward current contribute specifically to the gustatory AP and that the AP may be modulated by cyclic nucleotides. 展开更多
关键词 TASTE patch-clamps action potential potassium currents FORSKOLIN
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新兴评价构式“谁还不是NP”的话语互动功能及事件类型
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作者 武蕾旭 李思旭 《新疆大学学报(哲学社会科学版)》 北大核心 2025年第3期164-172,共9页
“谁还不是NP”是一个活跃在对话语境中的半图式新兴评价构式。该构式主要用在“陈述-反应”“评价-回应”“询问-回答”“质疑-反驳”相邻对中。话语互动功能在四类相邻对中分别是为陈述信息或回应信息提供理由、寻求认同、为询问或回... “谁还不是NP”是一个活跃在对话语境中的半图式新兴评价构式。该构式主要用在“陈述-反应”“评价-回应”“询问-回答”“质疑-反驳”相邻对中。话语互动功能在四类相邻对中分别是为陈述信息或回应信息提供理由、寻求认同、为询问或回答提供理由、直接进行反驳回应或为反驳回应提供理由。“谁还不是NP”在语境中主要涉及A-events、B-events、AB-events、AB-events四种事件信息类型,在不同相邻对中涉及的事件信息类型略有不同。 展开更多
关键词 评价构式 “谁还不是np 话语互动功能 事件类型
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