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松阿扁叶蜂SSR-PCR反应体系的优化
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作者 金娜 南小宁 贺虹 《湖北农业科学》 北大核心 2012年第18期4126-4128,4157,共4页
以SDS-蛋白酶K法提取的松阿扁叶蜂(Acantholyda posticalis Matsumura)基因组DNA为模板,利用L16(45)正交设计对影响松阿扁叶蜂SSR-PCR反应的主要参数DNA模板、Taq DNA聚合酶、Mg2+、引物和dNTPs进行优化。结果表明,松阿扁叶蜂SSR-PCR最... 以SDS-蛋白酶K法提取的松阿扁叶蜂(Acantholyda posticalis Matsumura)基因组DNA为模板,利用L16(45)正交设计对影响松阿扁叶蜂SSR-PCR反应的主要参数DNA模板、Taq DNA聚合酶、Mg2+、引物和dNTPs进行优化。结果表明,松阿扁叶蜂SSR-PCR最优的反应体系为25μL体系中含1.00U Taq DNA聚合酶、3.00mmol/L Mg2+、3.75mmol/L dNTPs、25.00ng/μL DNA模板和10.00μmol/L引物。 展开更多
关键词 松阿扁叶蜂(Acantholyda posticalis Matsumura) SSR-PCR 正交设计
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AN OBSERVATION OF FORMICA FUKAII WHEELER PREDATORY BEHAVIOR IN MONGOLIAN SCOTS PINE STANDS
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作者 Hou AijuNortheast Forestry UniversityLou Wei Yan DunliangInstitute of Protective Forest of Heilongjiang ProvinceTian JinquanStation of the Prevention and Quarantine of Forest Diseases and Pest of Nehe County 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1991年第2期100-104,共5页
The pine sawfly (Acantholyda posticalis Matsumura) is a serious pest of Pinus sylvestris; P. tabulaeformis and P. koraiensis. Chemical control has been generally applied, but caused a lot of problems. We first found F... The pine sawfly (Acantholyda posticalis Matsumura) is a serious pest of Pinus sylvestris; P. tabulaeformis and P. koraiensis. Chemical control has been generally applied, but caused a lot of problems. We first found Formica fukaii (new record in China). The ants nesting on Carex sp. preying on pine sawflics is an important predator. When nests are removed on purpose, the ant's behavior is normal and the nest are expanded. It is a dominant species and a natural enemy of pine sawfly in Nehe County. 展开更多
关键词 Formica fukaii CONTROL Acanlholyda posticalis Mongolian scots pine Artificial removing
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