To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hyb...To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hybrid B and their parental inbreds in the primary roots. By using 5′ end random primers in combination with three one-base-anchored primers, it was found that 22.5% and 22.9% of 877 total displayed cDNAs were differentially expressed between hybrid A, B and their parents, respectively. Both quantitative and qualitative differences in gene expression between hybrids and their parental inbreds were obvious, indicating that the patterns of gene expression in hybrids alter significantly as compared to their corresponding parents. On the other hand, by using MADS-box gene specific 5′ end primer for DDRT-PCR, we found that nearly all of the displayed cDNA fragments were polymorphic between hybrids and their parents, and major difference occurred in qualitative level, in which hybrid specific-expressed and silenced genes are the major two patterns, suggesting that MADS-box gene may be important for manifestation of differential gene expression and wheat heterosis. In comparison with our previous results by using seedling leaves, it is indicated that differential gene expression between hybrids and parents is dependent on the tissues tested, and more differentially expressed genes were observed in the primary roots than in the seedling leaves. Therefore, it is concluded that the expressions of both randomly displayed cDNAs and transcription factor genes, such as MADS-box, alter significantly between hybrids and their parents, which might be responsible for the observed heterosis.展开更多
[Objective] The aim was to research the effect of concentration of NO-3-N on root vigor and rhizosphere pH of winter wheat seedlings under water culture.[Method]By selecting Hoagland's nutritional solution as cult...[Objective] The aim was to research the effect of concentration of NO-3-N on root vigor and rhizosphere pH of winter wheat seedlings under water culture.[Method]By selecting Hoagland's nutritional solution as cultural medium and winter wheat as material of experiment,on the basis,testing root vigor,nutrient solution NO-3 and change of pH values under the different level of disposal,such as high(containing NO-3-N 15 mmol/L),medium(containing NO-3-N 7.5 mmol/L)and lower(containing NO-3-N 2.5 mmol/L).[Result]The results of this research showed that the effect of different nitrogen level on the wastage of nutrient solution NO-3,the changes of pH values and root vigor is obvious under the hydroponics condition.[Conclusion]Though NO-3 is a safe nitrogen sources when it was supplied to plants too more,it would restrain assimilation on nitrate nitrogen farther,but when it was supplied to plants too little,it would lead to deficiency of NO-3 that plants uptake and decrease of root activity,so it isn't useful to wheat young seedling to absorb nitrogen nutrition.展开更多
Objective] The aim was to study the effects of cotton straw extract on wheat root growth and define the al elopathic effect of cotton straw on wheat root. [Method] A sand culture experiment was conducted under differe...Objective] The aim was to study the effects of cotton straw extract on wheat root growth and define the al elopathic effect of cotton straw on wheat root. [Method] A sand culture experiment was conducted under different cotton straw ex-tract levels （0%, 1.0%, 2.0%, 4.0%, 8.0% and 10.0%）, and the morphology and physiological activity of wheat （Yangmai 13） root were studied. [Result] The cotton straw extract inhibited the elongation of wheat roots, but increased the root diame-ter, resulting in the decreased root surface area and volume; The extract treatments induced the increase of root reactive oxygen species （ROS） level, which led to an increased root membrane lipid peroxidation, thereby reducing the root vigor and in-hibiting the root water uptake and formation of biomass; With the increase of con-centration of cotton straw extract, the total phenolic acid content in the extract in-creased continuously, and the total phenolic acid content was closely related to the morphogenesis and physiological activity of wheat root. [Conclusion] The cotton straw extract inhibited the morphogenesis and physiological activity of wheat root through affecting the root ROS level, and the total phenolic content was one of the main limiting factors.展开更多
Studies on root development, soil physical properties, grain yield, and water-use efficiency are important for identifying suitable soil management practices for sustainable crop production. A field experiment was con...Studies on root development, soil physical properties, grain yield, and water-use efficiency are important for identifying suitable soil management practices for sustainable crop production. A field experiment was conducted from 2006 through 2008 in arid northwestern China to determine the effects of four tillage systems on soil properties, root development, water-use efficiency, and grain yield of winter wheat （Triticum aestivum L.）. The cultivar Fan 13 was grown under four tillage systems：conventional tillage （CT） without wheat stubble, no-tillage without wheat stubble mulching （NT）, no-tillage with wheat stubble standing （NTSS）, and no-tillage with wheat stubble mulching （NTS）. The soil bulk density （BD） under CT system increased gradually from sowing to harvest, but that in NT, NTSS, and NTS systems had little change. Compared to the CT system, the NTSS and NTS systems improved total soil water storage （0-150 cm） by 6.1-9.6 and 10.5- 15.3% before sowing, and by 2.2-8.9 and 13.0-15.1% after harvest, respectively. The NTSS and NTS systems also increased mean dry root weight density （DRWD） as compared to CT system. The NTS system significantly improved water-use efficiency by 17.2-17.5% and crop yield by 15.6-16.8%, and the NTSS system improved that by 7.8-9.6 and 7.0-12.8%, respectively, compared with the CT system. Our results suggested that Chinese farmers should consider adopting conservation tillage practices in arid northwestern China because of benefits to soil bulk density, water storage, root system, and winter wheat yield.展开更多
Effects of root penetration restriction on the growth and Mn nutrition of different wheat genotypes were studied in paddy soils using a method of nylon net bags (400 mesh) buried in the soil. The results showed that t...Effects of root penetration restriction on the growth and Mn nutrition of different wheat genotypes were studied in paddy soils using a method of nylon net bags (400 mesh) buried in the soil. The results showed that the spatial distribution of Mn in paddy soils played an important role in the growth and Mn nutrition of wheat crops. In the treatment where wheat roots were restricted in the plough layer by nylon net bags, the symptoms of Mn deficiency in wheat occurred much earlier and more seriously than usual. Of the two tested wheat genotypes, 80-8 was tolerant to Mn deficiency while 3295 was sensitive to Mn deficiency, respectively. The restriction of root penetration intensified symptoms of Mn deficiency of the Mn-deficient sensitive genotype (3295). The experiment demonstrated that well-developed roots with a strong ability to penetrate into the Mn-rich deep soil layer might explain the better tolerance of Mn deficiency in the tolerant genotypes.展开更多
A solution culture experiment was performed to investigate the effects of oxytetracycline （OTC） on wheat （Triticum aestivum L.） growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and...A solution culture experiment was performed to investigate the effects of oxytetracycline （OTC） on wheat （Triticum aestivum L.） growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and 80 mmol L-1 OTC. OTC is toxic to wheat. The wheat growth, especially wheat root was significantly decreased. Further OTC also significantly decreased root activity, chlorophyll contents, and photosynthetic parameters except for intercellular CO2 concentrations. The different responses of indicators such as root number, root activity and so on to OTC were also observed. The IC50 values for the tested indicators to OTC ranged from 7.1 to 113.4 mmol L-1 OTC. The order of indicator sensitivity to OTC was root number stomatal conductance chlorophyll a total chlorophyll photosynthetic rates total surface area transpiration rate chlorophyll b fresh weight of root dry weight of root total length dry weight of shoot = fresh weight of shoot total volume. The root number was more sensitive than other indicators with the IC50 value of 7.1 mmol L-1 OTC, and could be taken as the sensitive indicator to predict the hazards of OTC to wheat.展开更多
The irrigation method used in winter wheat fields affects micro-environment factors, such as relative humidity（RH） within canopy, soil temperature, topsoil bulk density, soil matric potential, and soil nutrients, an...The irrigation method used in winter wheat fields affects micro-environment factors, such as relative humidity（RH） within canopy, soil temperature, topsoil bulk density, soil matric potential, and soil nutrients, and these changes may affect plant root growth.An experiment was carried out to explore the effects of irrigation method on micro-environments and root distribution in a winter wheat field in the 2007–2008 and 2008–2009 growing seasons.The results showed that border irrigation（BI）, sprinkler irrigation（SI）, and surface drip irrigation（SDI） had no significant effects on soil temperature.Topsoil bulk density, RH within the canopy, soil available N distribution, and soil matric potential were significantly affected by the three treatments.The change in soil matric potential was the key reason for the altered root profile distribution patterns.Additionally, more fine roots were produced in the BI treatment when soil water content was low and topsoil bulk density was high.Root growth was most stimulated in the top soil layers and inhibited in the deep layers in the SDI treatment, followed by SI and BI, which was due to the different water application frequencies.As a result, the root profile distribution differed, depending on the irrigation method used.The root distribution pattern changes could be described by the power level variation in the exponential function.A good knowledge of root distribution patterns is important when attempting to model water and nutrient movements and when studying soil-plant interactions.展开更多
Two pot experiments were conducted to study the effects of root pruning at the stem elongation stage on non-hydraulic root-sourced signals （nHRS）, drought tolerance and water use efficiency of winter wheat （Triticu...Two pot experiments were conducted to study the effects of root pruning at the stem elongation stage on non-hydraulic root-sourced signals （nHRS）, drought tolerance and water use efficiency of winter wheat （Triticum aestivum）. The root pruning significantly reduced the root weight of wheat, but had no effect on root/shoot ratio at the two tested stages. At booting stage, specific root respiration of root pruned plants was significantly higher than those with intact roots （1.06 and 0.94 mmol g-1 s-1, respectively）. The soil water content （SWC） at which nHRS for root pruned plants appeared was higher and terminated lower than for intact root plants, the threshold range of nHRS was markedly greater for root pruned plants （61.1-44.6% field water capacity） than for intact root plants （57.9-46.1% field water capacity）. At flowering stage, while there was no significant difference in specific root respiration. The SWCs at which nHRS appeared and terminated were both higher for root pruned plants than for intact root plants. The values of chlorophyll fluorescence parameters, i.e., the effective photosystem II quantum yield （F PS II ）, the maximum photochemical efficiency of PS II （F v /F m ）, coefficient of photochemical quenching （qP）, and coefficient of non-photochemical quenching （NPQ）, in root pruned plants were significantly higher than in intact root plants, 7 d after withholding of water. Root pruned plants had significantly higher water use efficiency （WUE） than intact root plants in well-watered and medium drought soil, but not in severe drought condition. In addition, root pruning had no significant effect on grain yield in well-watered and medium drought soil, but significantly decreased grain yield in severe drought condition. In conclusion, the current study showed that root pruning significantly altered nHRS sensitivity and improved WUE of winter wheat in well-watered and medium drought soil, but lowered drought tolerance of winter wheat in severe drought soil. This suggests a possible direction of drought- resistance breeding and potential agricultural measure to improve WUE of winter wheat under semiarid conditions.展开更多
The objective of this study was to understand the morphological,physiological,and molecular responses of wheat roots to nitrate supply at seedling stage.Two wheat genotypes,Jimai 22 and Shannong 15,were grown in Hoagl...The objective of this study was to understand the morphological,physiological,and molecular responses of wheat roots to nitrate supply at seedling stage.Two wheat genotypes,Jimai 22 and Shannong 15,were grown in Hoagland＇s nutrient solution with different nitrate levels at seedling stage.Results indicated that the plant dry weight and N accumulation increased with the increase of nitrate supply.The number of axial root,total uptake area （TUA）,and active uptake area （AUA） increased with more nitrate supply.Correlation analysis indicated that significant positive correlations existed between N accumulation and dry weight,N accumulation and AUA,and N accumulation and AUA/TUA.Although,the expressions of NRT2.1,NRT2.2,and NRT2.3 decreased with nitrate supply increased,the expressions of NRT1,NRT2.1,and NRT2.3 could maintain high level at N3 treatment.The free amino acid and NO3- content in shoot also increased with the increased nitrate application,but no significant difference was found in root among the treatments.These results implied that the increase of N uptake by nitrate supply was due to the morphological and physiological responses of wheat roots and the high expression level of TaNRT genes.Similarly,the contribution of morphological,physiological,and molecular parameters was different between two genotypes of wheat.展开更多
To reveal the period and after-effect of soil water stress on winter wheat, the article employs the experiment results carried out in the greenhouse. The results showed that the root-restricted weights varied with str...To reveal the period and after-effect of soil water stress on winter wheat, the article employs the experiment results carried out in the greenhouse. The results showed that the root-restricted weights varied with stress degrees and stress times during and after water stressing. In the course of stress, the chief reason resticting the weight of root was the stress intensity at this time, and that of severe stress treatment was larger than that of mild stress treatment. After water stress was relieved, the results of the after-effect of soil water stress on root growth were that, the stress intensity of short-time and mild stress was larger than that of long-time and severe stress. Comparing two-stage stress intensities, root-restricted weight resulted from after-effect intensity of stress under all of the short-time treatment, and the mild and the long-time stress treatments, while that resulted from the period stress intensity under the severe and the long-time stress treatments. In general, the effects of water stress on root were attributed to the three factors, a formed basis in the previous stage, the after-effect of water condition before this stage and influence of water in this stage, which lead to the characters of root in the whole growth stage.展开更多
Synthetic hexaploid wheat(SHW),possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties.We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived ...Synthetic hexaploid wheat(SHW),possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties.We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line(SHW-L1)and a common wheat line,under normal(NC)and polyethylene glycol-simulated drought stress conditions(DC).We mapped quantitative trait loci(QTLs)for root traits using an enriched high-density genetic map containing 120370 single nucleotide polymorphisms(SNPs),733 diversity arrays technology markers(DArT)and 119 simple sequence repeats(SSRs).With four replicates per treatment,we identified 19 QTLs for root traits under NC and DC,and 12 of them could be consistently detected with three or four replicates.Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7%of the phenotypic variation respectively,and six novel QTLs for root fresh weight,the ratio of root water loss,total root surface area,number of root tips,and number of root forks under DC explained 8.5–14%of the phenotypic variation.Here seven of eight novel QTLs could be consistently detected with more than three replicates.Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.展开更多
Differential expression of gene in iron-efficient wheat cultivar Jing411 and iron-inefficient cul-tivar SanshumaiS under iron-deficiency and iron-sufficiency conditions was revealed by differential display reverse tra...Differential expression of gene in iron-efficient wheat cultivar Jing411 and iron-inefficient cul-tivar SanshumaiS under iron-deficiency and iron-sufficiency conditions was revealed by differential display reverse transcript PCR (DDRT-PCR) method. Northern blotting was carried out using ATP-binding transporter (ABC) cDNA obtained from DDRT-PCR products of the cultivar Jing411 as probe. Our results suggested that ABC gene expression was suppressed under iron-deficiency condition.展开更多
Drought priming is a promising approach to improve tolerance to further drought in wheat.The root apex plays important roles in drought however,its contribution to drought priming remains unknown.To provide mechanisti...Drought priming is a promising approach to improve tolerance to further drought in wheat.The root apex plays important roles in drought however,its contribution to drought priming remains unknown.To provide mechanistic insights into this process,the transcriptomes and proteomes at three different zones along the root axis under drought stress were analyzed.Physiological assessment of root growth indicated that priming augmented roots growth in response to drought and also the levels of protective proline and glycine betaine.Scanning across the proximal to the distal zones of the root apex indicated increases the transcription of genes involved in primary and secondary metabolism.Conversely,genes related to translation,transcription,folding,sorting and degradation,replication and repair were increased in the apex compared to the proximal zone.A single drought episode suppressed their expression but prior drought priming served to maintain expression with recurrent drought stress.The differentially primed responses genes were mainly involved in the pathways related to plant hormone signaling,stress defense and cell wall modification.The prediction of regulatory hubs using Cytoscape implicated signaling components such as the ABA receptor PYL4 as influencing antioxidant status and the cell cycle.Based our integrative transcriptomic-proteomic assessments we present a model for drought priming protected plant hormone signaling transduction pathways to drive the cell cycle and cell wall loosening to confer beneficial effects on roots to counter the effects of drought.This model provides a theoretical basis for improvement of drought tolerance in wheat,via an increased understanding of drought priming induced drought tolerance.展开更多
Yield losses of bread wheat due to crown rot can be more severe when drought conditions occur during the grain-filling period.Root architecture characteristics are important for soil exploration and belowground resour...Yield losses of bread wheat due to crown rot can be more severe when drought conditions occur during the grain-filling period.Root architecture characteristics are important for soil exploration and belowground resource acquisition and are essential for adaptation to water-limited environments.Traits such as root angle,length and density have been strongly associated with acquisition efficiency and contribute to yield stability of the crop.The impact of crown rot pathogens on wheat root architecture is poorly understood.We examined differences in root angle,length and number,as well as dry root weight of the crown rot-susceptible bread wheat cultivar,Livingston inoculated with one of two crown rot pathogens Fusarium culmorum or Fusarium pseudograminearum in a transparent-sided root observation chamber.Significant adverse impacts on plant health and growth were revealed by visual discolouration of the leaf sheaths;fresh and dry shoot weight;leaf area of the oldest and the youngest fully expanded leaf and leaf number.Values of most recorded root system measurements were reduced when inoculated with either F.culmorum or F.pseudograminearum.In contrast,root angle was increased in the presence of F.culmorum but was not significantly changed by F.pseudograminearum.The development of whiteheads and grain losses in bread wheat caused by crown rot have previously been associated with blockages of the vascular systems.The method employed here was able to identify differences in the pathogen impacts on roots,which were not detected using previous systems.This research indicates that in the presence of F.culmorum and F.pseudograminearum infection,not only reductions in the size and biomass of the shoot system but also changes in the length,biomass and architecture of the root system could play an important role in yield loss.展开更多
Root growth traits for different wheat types varied during the growth cycle. The root system of 93 Zhong 6, which is a dwarf, big-ear variety, reached its highest density at anthesis, while the root density of Zhoumai...Root growth traits for different wheat types varied during the growth cycle. The root system of 93 Zhong 6, which is a dwarf, big-ear variety, reached its highest density at anthesis, while the root density of Zhoumai 13, a medium-type variety, demonsrated its highest value during the node elongation stage and decreased rapidly at later growth stages, which resulted in lower yield. The root density of Zhongyu 6 and 98 Zhong 18, high yield potential, multiple ears varieties, did not show observable variation in their root systems during their growth cycles.展开更多
Fusarium crown rot(FCR) is a soilborne disease causing severe yield losses in many wheat-growing areas of the world. Diseased plants show browning and necrosis of roots and stems causing white heads at maturity. Littl...Fusarium crown rot(FCR) is a soilborne disease causing severe yield losses in many wheat-growing areas of the world. Diseased plants show browning and necrosis of roots and stems causing white heads at maturity. Little is known about the molecular processes employed by wheat roots to respond to the disease. We characterized morphological, transcriptional and hormonal changes in wheat seedling roots following challenge with Fusarium pseudograminearum(Fp), the main pathogen of FCR. The pathogen inhibited root development to various extents depending on plants' resistance level. Many genes responsive to FCR infection in wheat roots were enriched in plant hormone pathways. The contents of compounds involved in biosynthesis and metabolism of jasmonic acid, salicylic acid, cytokinin and auxin were drastically changed in roots at five days post-inoculation. Presoaking seeds in methyl jasmonate for 24 h promoted FCR resistance, whereas presoaking with cytokinin 6-benzylaminopurine made plants more susceptible. Overexpression of TaOPR3, a gene involved in jasmonic acid biosynthesis, enhanced plant resistance as well as root and shoot growth during infection.展开更多
The equilibrium between root, shoot and growth stability under different soil water conditions were investigated in a tube experiment of winter wheat. The water supplying treatments included： sufficient irrigation at...The equilibrium between root, shoot and growth stability under different soil water conditions were investigated in a tube experiment of winter wheat. The water supplying treatments included： sufficient irrigation at whole growth phase, moderate deficiency irrigation at whole growth phase, serious deficiency irrigation at whole growth phase, sufficient irrigation at jointing stage, tillering stage, flowering stage, and fillering respectively, after moderate and serious water deficit during their previous growth stage. Root and shoot biomass were measured. On the basis of the cooperative root-shoot interactions model, the equilibrium and growth stability were studied on the strength of the kinetics system theory. There was only one varying equilibrium point between the root and shoot over the life time of the winter wheat plant. Water stress prolonged the duration of stable growth, the more serious the water deficit, the longer the period of stable growth. The duration of stable growth was shortened and that of unstable growth was prolonged after water recovery. The growth behavior of the plants exposed to moderate water deficit shifted from stable to unstable until the end of the growth, after rewatering at flowering. In the life-time of the crop, the root and shoot had been adjusting themselves in structure and function so as to maintain an equilibrium, but could not achieve the equilibrium state for long. They were always in an unbalanced state from the beginning to the end of growth. This was the essence of root-shoot equilibrium. Water stress inhibited the function of root and shoot, reduced root shoot interactions, and as a result, the plant growth gradually tended to stabilize. Rewatering enhanced root shoot interactions, prolonged duration of instable growth. Rewatering at flowering could upset the inherent relativity during the long time of stable growth from flowering to filling stage, thus leading to unstable growth and enhanced dry matter accumulating rate in the whole plant.展开更多
Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is respons...Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is responsible for water and nutrient acquisition,thus crucial for competitive fitness and crop yield in challenging environments(Karlova et al.,2021;Liu et al.,2022).Over the past decades,extensive research has focused on identifying genes accountable for root growth and development in plants(Rogers and Benfey,2015).However,only a limited number of genes have been cloned in wheat(Li et al.,2021).展开更多
To elucidate the response of oxidative metabolism,triggered by elevated ammonium(NH_(4)^(+))concentrations,on root growth of wheat seedlings,Yumai 49(NH_(4)^(+)-tolerant)and Lumai 15(NH_(4)^(+)-sensitive)cultivars wer...To elucidate the response of oxidative metabolism,triggered by elevated ammonium(NH_(4)^(+))concentrations,on root growth of wheat seedlings,Yumai 49(NH_(4)^(+)-tolerant)and Lumai 15(NH_(4)^(+)-sensitive)cultivars were supplied with either 5.0 mmol L^(–1)NH_(4)^(+)-N(EAC)or 5.0 mmol L–1 NO_(3)–-N(CON)under hydroponic conditions.Root growth in both cultivars was significantly reduced under EAC,and the negative effect was greater in Lumai 15.EAC enhanced the activities of monodehydroascorbate reductase and dehydroascorbate reductase in the roots of both cultivars,while it decreased ascorbic acid(ASA)content and GDP-mannose pyrophosphorylase(GMPase)activity at the 12 th day after treatment in Lumai 15 by 62.0 and 71.4%;and in Yumai 49 by 38.8 and 62.2%,respectively,indicating that the regeneration of ASA was increased,but the biosynthesis of ASA was reduced under EAC treatment.Moreover,EAC increased DHA/ASA,reactive oxygen species(ROS),and malondialdehyde contents,as well as antioxidant enzyme activities in the roots of both cultivars.Relatively greater increases in ROS and soluble sugar,and lower antioxidant enzyme activities in Lumai 15 indicate severe disruption of oxidative metabolism when compared to Yumai 49.Results reveal that the reduction of ASA biosynthesis via decreased GMPase activity under the EAC condition probably acts as a trigger for accumulated ROS and imbalanced redox status,resulting in root growth inhibition during wheat seedling growth stage.Yumai 49,being an NH_(4)^(+)-tolerant cultivar,had the stronger capacity to protect itself from oxidative stress,which allowed it to retain a lower DHA to ASA ratio by maintaining a better redox homeostasis than could be maintained in the NH_(4)^(+)-sensitive cultivar Lumai 15.展开更多
The root system architecture(RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive response...The root system architecture(RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat(Triticum aestivum L.). Here, a high-throughput germination paper-based plant phenotyping system was used to identify seedling traits in a wheat doubled haploid mapping population, Savannah×Rialto. Significant genotypic and nitrate-N treatment variation was found across the population for seedling traits with distinct trait grouping for root size-related traits and root distribution-related traits. Quantitative trait locus(QTL) analysis identified a total of 59 seedling trait QTLs. Across two nitrate treatments, 27 root QTLs were specific to the nitrate treatment. Transcriptomic analyses for one of the QTLs on chromosome 2 D, which was found under low nitrate conditions, revealed gene enrichment in N-related biological processes and 28 differentially expressed genes with possible involvement in a root angle response. Together, these findings provide genetic insight into root system architecture and plant adaptive responses to nitrate, as well as targets that could help improve N capture in wheat.展开更多
文摘To provide an insight into the molecular basis of heterosis, differential display of mRNA was used to analyze the difference of gene expression between wheat (Triticum aestivum L.) heterotic hybrid A, nonheterotic hybrid B and their parental inbreds in the primary roots. By using 5′ end random primers in combination with three one-base-anchored primers, it was found that 22.5% and 22.9% of 877 total displayed cDNAs were differentially expressed between hybrid A, B and their parents, respectively. Both quantitative and qualitative differences in gene expression between hybrids and their parental inbreds were obvious, indicating that the patterns of gene expression in hybrids alter significantly as compared to their corresponding parents. On the other hand, by using MADS-box gene specific 5′ end primer for DDRT-PCR, we found that nearly all of the displayed cDNA fragments were polymorphic between hybrids and their parents, and major difference occurred in qualitative level, in which hybrid specific-expressed and silenced genes are the major two patterns, suggesting that MADS-box gene may be important for manifestation of differential gene expression and wheat heterosis. In comparison with our previous results by using seedling leaves, it is indicated that differential gene expression between hybrids and parents is dependent on the tissues tested, and more differentially expressed genes were observed in the primary roots than in the seedling leaves. Therefore, it is concluded that the expressions of both randomly displayed cDNAs and transcription factor genes, such as MADS-box, alter significantly between hybrids and their parents, which might be responsible for the observed heterosis.
基金Supported by the Key Programfromthe National Natural Science Foundation of China(30230230)the National Natural Science Foundation Agricultural Program of China(30070429)Scientific Research Program for Universities in Inner Mongolia Autonomous Region(NJZY07120)~~
文摘[Objective] The aim was to research the effect of concentration of NO-3-N on root vigor and rhizosphere pH of winter wheat seedlings under water culture.[Method]By selecting Hoagland's nutritional solution as cultural medium and winter wheat as material of experiment,on the basis,testing root vigor,nutrient solution NO-3 and change of pH values under the different level of disposal,such as high(containing NO-3-N 15 mmol/L),medium(containing NO-3-N 7.5 mmol/L)and lower(containing NO-3-N 2.5 mmol/L).[Result]The results of this research showed that the effect of different nitrogen level on the wastage of nutrient solution NO-3,the changes of pH values and root vigor is obvious under the hydroponics condition.[Conclusion]Though NO-3 is a safe nitrogen sources when it was supplied to plants too more,it would restrain assimilation on nitrate nitrogen farther,but when it was supplied to plants too little,it would lead to deficiency of NO-3 that plants uptake and decrease of root activity,so it isn't useful to wheat young seedling to absorb nitrogen nutrition.
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund(CX(12)3034)Jiangsu Three Agricultural Innovation Project(SXGC[2014]299)National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2014BAD11B02)~~
文摘Objective] The aim was to study the effects of cotton straw extract on wheat root growth and define the al elopathic effect of cotton straw on wheat root. [Method] A sand culture experiment was conducted under different cotton straw ex-tract levels （0%, 1.0%, 2.0%, 4.0%, 8.0% and 10.0%）, and the morphology and physiological activity of wheat （Yangmai 13） root were studied. [Result] The cotton straw extract inhibited the elongation of wheat roots, but increased the root diame-ter, resulting in the decreased root surface area and volume; The extract treatments induced the increase of root reactive oxygen species （ROS） level, which led to an increased root membrane lipid peroxidation, thereby reducing the root vigor and in-hibiting the root water uptake and formation of biomass; With the increase of con-centration of cotton straw extract, the total phenolic acid content in the extract in-creased continuously, and the total phenolic acid content was closely related to the morphogenesis and physiological activity of wheat root. [Conclusion] The cotton straw extract inhibited the morphogenesis and physiological activity of wheat root through affecting the root ROS level, and the total phenolic content was one of the main limiting factors.
基金funded by the Ph D Programs Foundation, Ministry of Education, China(20106202110002)the National Public Welfare Foundation for Industry Scheme of China (201103001)the National Natural Science Foundation of China (201131160265)
文摘Studies on root development, soil physical properties, grain yield, and water-use efficiency are important for identifying suitable soil management practices for sustainable crop production. A field experiment was conducted from 2006 through 2008 in arid northwestern China to determine the effects of four tillage systems on soil properties, root development, water-use efficiency, and grain yield of winter wheat （Triticum aestivum L.）. The cultivar Fan 13 was grown under four tillage systems：conventional tillage （CT） without wheat stubble, no-tillage without wheat stubble mulching （NT）, no-tillage with wheat stubble standing （NTSS）, and no-tillage with wheat stubble mulching （NTS）. The soil bulk density （BD） under CT system increased gradually from sowing to harvest, but that in NT, NTSS, and NTS systems had little change. Compared to the CT system, the NTSS and NTS systems improved total soil water storage （0-150 cm） by 6.1-9.6 and 10.5- 15.3% before sowing, and by 2.2-8.9 and 13.0-15.1% after harvest, respectively. The NTSS and NTS systems also increased mean dry root weight density （DRWD） as compared to CT system. The NTS system significantly improved water-use efficiency by 17.2-17.5% and crop yield by 15.6-16.8%, and the NTSS system improved that by 7.8-9.6 and 7.0-12.8%, respectively, compared with the CT system. Our results suggested that Chinese farmers should consider adopting conservation tillage practices in arid northwestern China because of benefits to soil bulk density, water storage, root system, and winter wheat yield.
文摘Effects of root penetration restriction on the growth and Mn nutrition of different wheat genotypes were studied in paddy soils using a method of nylon net bags (400 mesh) buried in the soil. The results showed that the spatial distribution of Mn in paddy soils played an important role in the growth and Mn nutrition of wheat crops. In the treatment where wheat roots were restricted in the plough layer by nylon net bags, the symptoms of Mn deficiency in wheat occurred much earlier and more seriously than usual. Of the two tested wheat genotypes, 80-8 was tolerant to Mn deficiency while 3295 was sensitive to Mn deficiency, respectively. The restriction of root penetration intensified symptoms of Mn deficiency of the Mn-deficient sensitive genotype (3295). The experiment demonstrated that well-developed roots with a strong ability to penetrate into the Mn-rich deep soil layer might explain the better tolerance of Mn deficiency in the tolerant genotypes.
基金supported by the National Natural Science Foundation of China (40701163)the Natural Science Foundation of Beijing City, China (6092019)+1 种基金the International Foundation for Science, Stockholm, Sweden, the Organization for the Prohibition of Chemical Weapons, the Hague, and the Netherlands, through a grant to Li Zhaojun (C/4076)the National Basic Research Program of China (973 Program, 2007CB109305)
文摘A solution culture experiment was performed to investigate the effects of oxytetracycline （OTC） on wheat （Triticum aestivum L.） growth, chlorophyll contents, and photosynthesis at five levels of 0, 10, 20, 40, and 80 mmol L-1 OTC. OTC is toxic to wheat. The wheat growth, especially wheat root was significantly decreased. Further OTC also significantly decreased root activity, chlorophyll contents, and photosynthetic parameters except for intercellular CO2 concentrations. The different responses of indicators such as root number, root activity and so on to OTC were also observed. The IC50 values for the tested indicators to OTC ranged from 7.1 to 113.4 mmol L-1 OTC. The order of indicator sensitivity to OTC was root number stomatal conductance chlorophyll a total chlorophyll photosynthetic rates total surface area transpiration rate chlorophyll b fresh weight of root dry weight of root total length dry weight of shoot = fresh weight of shoot total volume. The root number was more sensitive than other indicators with the IC50 value of 7.1 mmol L-1 OTC, and could be taken as the sensitive indicator to predict the hazards of OTC to wheat.
基金the National Natural Science Foundation of China(51109214,31101074 and 51309211)the National Key Technology Research and Development Program of China(2011BAD32B)the Basic Scientific Research Foundation of National Non-Profit Scientific Institute of China(BSRF201303)
文摘The irrigation method used in winter wheat fields affects micro-environment factors, such as relative humidity（RH） within canopy, soil temperature, topsoil bulk density, soil matric potential, and soil nutrients, and these changes may affect plant root growth.An experiment was carried out to explore the effects of irrigation method on micro-environments and root distribution in a winter wheat field in the 2007–2008 and 2008–2009 growing seasons.The results showed that border irrigation（BI）, sprinkler irrigation（SI）, and surface drip irrigation（SDI） had no significant effects on soil temperature.Topsoil bulk density, RH within the canopy, soil available N distribution, and soil matric potential were significantly affected by the three treatments.The change in soil matric potential was the key reason for the altered root profile distribution patterns.Additionally, more fine roots were produced in the BI treatment when soil water content was low and topsoil bulk density was high.Root growth was most stimulated in the top soil layers and inhibited in the deep layers in the SDI treatment, followed by SI and BI, which was due to the different water application frequencies.As a result, the root profile distribution differed, depending on the irrigation method used.The root distribution pattern changes could be described by the power level variation in the exponential function.A good knowledge of root distribution patterns is important when attempting to model water and nutrient movements and when studying soil-plant interactions.
基金supported by the Fund of State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau,China(10501-1201)the Key Technologies R&D Program of China during the 11th Five-Year Plan period(2012BAD14B08)the Innovation Team Program,Ministry of Education of China
文摘Two pot experiments were conducted to study the effects of root pruning at the stem elongation stage on non-hydraulic root-sourced signals （nHRS）, drought tolerance and water use efficiency of winter wheat （Triticum aestivum）. The root pruning significantly reduced the root weight of wheat, but had no effect on root/shoot ratio at the two tested stages. At booting stage, specific root respiration of root pruned plants was significantly higher than those with intact roots （1.06 and 0.94 mmol g-1 s-1, respectively）. The soil water content （SWC） at which nHRS for root pruned plants appeared was higher and terminated lower than for intact root plants, the threshold range of nHRS was markedly greater for root pruned plants （61.1-44.6% field water capacity） than for intact root plants （57.9-46.1% field water capacity）. At flowering stage, while there was no significant difference in specific root respiration. The SWCs at which nHRS appeared and terminated were both higher for root pruned plants than for intact root plants. The values of chlorophyll fluorescence parameters, i.e., the effective photosystem II quantum yield （F PS II ）, the maximum photochemical efficiency of PS II （F v /F m ）, coefficient of photochemical quenching （qP）, and coefficient of non-photochemical quenching （NPQ）, in root pruned plants were significantly higher than in intact root plants, 7 d after withholding of water. Root pruned plants had significantly higher water use efficiency （WUE） than intact root plants in well-watered and medium drought soil, but not in severe drought condition. In addition, root pruning had no significant effect on grain yield in well-watered and medium drought soil, but significantly decreased grain yield in severe drought condition. In conclusion, the current study showed that root pruning significantly altered nHRS sensitivity and improved WUE of winter wheat in well-watered and medium drought soil, but lowered drought tolerance of winter wheat in severe drought soil. This suggests a possible direction of drought- resistance breeding and potential agricultural measure to improve WUE of winter wheat under semiarid conditions.
基金supported by the National Natural Science Foundation of China (30871477)the National Basic Research Program of China (973 Program,2009CB118602)the National Department of Public Benefit Research Foundation,China (200803037)
文摘The objective of this study was to understand the morphological,physiological,and molecular responses of wheat roots to nitrate supply at seedling stage.Two wheat genotypes,Jimai 22 and Shannong 15,were grown in Hoagland＇s nutrient solution with different nitrate levels at seedling stage.Results indicated that the plant dry weight and N accumulation increased with the increase of nitrate supply.The number of axial root,total uptake area （TUA）,and active uptake area （AUA） increased with more nitrate supply.Correlation analysis indicated that significant positive correlations existed between N accumulation and dry weight,N accumulation and AUA,and N accumulation and AUA/TUA.Although,the expressions of NRT2.1,NRT2.2,and NRT2.3 decreased with nitrate supply increased,the expressions of NRT1,NRT2.1,and NRT2.3 could maintain high level at N3 treatment.The free amino acid and NO3- content in shoot also increased with the increased nitrate application,but no significant difference was found in root among the treatments.These results implied that the increase of N uptake by nitrate supply was due to the morphological and physiological responses of wheat roots and the high expression level of TaNRT genes.Similarly,the contribution of morphological,physiological,and molecular parameters was different between two genotypes of wheat.
基金the Ministry of Science and Technology of China （1999011700） the National Natural Science Foundation of China （49971042）.
文摘To reveal the period and after-effect of soil water stress on winter wheat, the article employs the experiment results carried out in the greenhouse. The results showed that the root-restricted weights varied with stress degrees and stress times during and after water stressing. In the course of stress, the chief reason resticting the weight of root was the stress intensity at this time, and that of severe stress treatment was larger than that of mild stress treatment. After water stress was relieved, the results of the after-effect of soil water stress on root growth were that, the stress intensity of short-time and mild stress was larger than that of long-time and severe stress. Comparing two-stage stress intensities, root-restricted weight resulted from after-effect intensity of stress under all of the short-time treatment, and the mild and the long-time stress treatments, while that resulted from the period stress intensity under the severe and the long-time stress treatments. In general, the effects of water stress on root were attributed to the three factors, a formed basis in the previous stage, the after-effect of water condition before this stage and influence of water in this stage, which lead to the characters of root in the whole growth stage.
基金supported by the National Natural Science Foundation of China(31771794,91731305 and 31560388)the outstanding Youth Foundation of the Department of Science and Technology of Sichuan Province,China(2016JQ0040)+1 种基金the Key Technology Research and Development Program of the Department of Science and Technology of Sichuan Province,China(2016NZ0057)the International Science&Technology Cooperation Program of the Bureau of Science and Technology of Chengdu,China(2015DFA306002015-GH03-00008-HZ)。
文摘Synthetic hexaploid wheat(SHW),possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties.We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line(SHW-L1)and a common wheat line,under normal(NC)and polyethylene glycol-simulated drought stress conditions(DC).We mapped quantitative trait loci(QTLs)for root traits using an enriched high-density genetic map containing 120370 single nucleotide polymorphisms(SNPs),733 diversity arrays technology markers(DArT)and 119 simple sequence repeats(SSRs).With four replicates per treatment,we identified 19 QTLs for root traits under NC and DC,and 12 of them could be consistently detected with three or four replicates.Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7%of the phenotypic variation respectively,and six novel QTLs for root fresh weight,the ratio of root water loss,total root surface area,number of root tips,and number of root forks under DC explained 8.5–14%of the phenotypic variation.Here seven of eight novel QTLs could be consistently detected with more than three replicates.Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.
文摘Differential expression of gene in iron-efficient wheat cultivar Jing411 and iron-inefficient cul-tivar SanshumaiS under iron-deficiency and iron-sufficiency conditions was revealed by differential display reverse transcript PCR (DDRT-PCR) method. Northern blotting was carried out using ATP-binding transporter (ABC) cDNA obtained from DDRT-PCR products of the cultivar Jing411 as probe. Our results suggested that ABC gene expression was suppressed under iron-deficiency condition.
基金supported by the National Key Research and Development Program of China (2016YFD0300107)the National Natural Science Foundation of China (31771693, U1803235)+3 种基金the China Agriculture Research System (CARS-03)JCIC-MCPthe 111 Project (B16026)the UK Biotechnology and Biological Sciences Research Council (BBSRC) Exchange Grant (BB/R02118X/1)。
文摘Drought priming is a promising approach to improve tolerance to further drought in wheat.The root apex plays important roles in drought however,its contribution to drought priming remains unknown.To provide mechanistic insights into this process,the transcriptomes and proteomes at three different zones along the root axis under drought stress were analyzed.Physiological assessment of root growth indicated that priming augmented roots growth in response to drought and also the levels of protective proline and glycine betaine.Scanning across the proximal to the distal zones of the root apex indicated increases the transcription of genes involved in primary and secondary metabolism.Conversely,genes related to translation,transcription,folding,sorting and degradation,replication and repair were increased in the apex compared to the proximal zone.A single drought episode suppressed their expression but prior drought priming served to maintain expression with recurrent drought stress.The differentially primed responses genes were mainly involved in the pathways related to plant hormone signaling,stress defense and cell wall modification.The prediction of regulatory hubs using Cytoscape implicated signaling components such as the ABA receptor PYL4 as influencing antioxidant status and the cell cycle.Based our integrative transcriptomic-proteomic assessments we present a model for drought priming protected plant hormone signaling transduction pathways to drive the cell cycle and cell wall loosening to confer beneficial effects on roots to counter the effects of drought.This model provides a theoretical basis for improvement of drought tolerance in wheat,via an increased understanding of drought priming induced drought tolerance.
基金supported by a USQ Postgraduate Research Scholarship and the Queensland Government。
文摘Yield losses of bread wheat due to crown rot can be more severe when drought conditions occur during the grain-filling period.Root architecture characteristics are important for soil exploration and belowground resource acquisition and are essential for adaptation to water-limited environments.Traits such as root angle,length and density have been strongly associated with acquisition efficiency and contribute to yield stability of the crop.The impact of crown rot pathogens on wheat root architecture is poorly understood.We examined differences in root angle,length and number,as well as dry root weight of the crown rot-susceptible bread wheat cultivar,Livingston inoculated with one of two crown rot pathogens Fusarium culmorum or Fusarium pseudograminearum in a transparent-sided root observation chamber.Significant adverse impacts on plant health and growth were revealed by visual discolouration of the leaf sheaths;fresh and dry shoot weight;leaf area of the oldest and the youngest fully expanded leaf and leaf number.Values of most recorded root system measurements were reduced when inoculated with either F.culmorum or F.pseudograminearum.In contrast,root angle was increased in the presence of F.culmorum but was not significantly changed by F.pseudograminearum.The development of whiteheads and grain losses in bread wheat caused by crown rot have previously been associated with blockages of the vascular systems.The method employed here was able to identify differences in the pathogen impacts on roots,which were not detected using previous systems.This research indicates that in the presence of F.culmorum and F.pseudograminearum infection,not only reductions in the size and biomass of the shoot system but also changes in the length,biomass and architecture of the root system could play an important role in yield loss.
文摘Root growth traits for different wheat types varied during the growth cycle. The root system of 93 Zhong 6, which is a dwarf, big-ear variety, reached its highest density at anthesis, while the root density of Zhoumai 13, a medium-type variety, demonsrated its highest value during the node elongation stage and decreased rapidly at later growth stages, which resulted in lower yield. The root density of Zhongyu 6 and 98 Zhong 18, high yield potential, multiple ears varieties, did not show observable variation in their root systems during their growth cycles.
基金supported by the State Key Laboratory of North China Crop Improvement and RegulationNational Key Research and Development Program of China (2018YFD0300501)National Natural Science Foundation of China (31872865)。
文摘Fusarium crown rot(FCR) is a soilborne disease causing severe yield losses in many wheat-growing areas of the world. Diseased plants show browning and necrosis of roots and stems causing white heads at maturity. Little is known about the molecular processes employed by wheat roots to respond to the disease. We characterized morphological, transcriptional and hormonal changes in wheat seedling roots following challenge with Fusarium pseudograminearum(Fp), the main pathogen of FCR. The pathogen inhibited root development to various extents depending on plants' resistance level. Many genes responsive to FCR infection in wheat roots were enriched in plant hormone pathways. The contents of compounds involved in biosynthesis and metabolism of jasmonic acid, salicylic acid, cytokinin and auxin were drastically changed in roots at five days post-inoculation. Presoaking seeds in methyl jasmonate for 24 h promoted FCR resistance, whereas presoaking with cytokinin 6-benzylaminopurine made plants more susceptible. Overexpression of TaOPR3, a gene involved in jasmonic acid biosynthesis, enhanced plant resistance as well as root and shoot growth during infection.
基金supported by the National Basic Research Program of China(973 Program,G1999011709)the Natural Science Foundation of Guangdong Province,China(33135).
文摘The equilibrium between root, shoot and growth stability under different soil water conditions were investigated in a tube experiment of winter wheat. The water supplying treatments included： sufficient irrigation at whole growth phase, moderate deficiency irrigation at whole growth phase, serious deficiency irrigation at whole growth phase, sufficient irrigation at jointing stage, tillering stage, flowering stage, and fillering respectively, after moderate and serious water deficit during their previous growth stage. Root and shoot biomass were measured. On the basis of the cooperative root-shoot interactions model, the equilibrium and growth stability were studied on the strength of the kinetics system theory. There was only one varying equilibrium point between the root and shoot over the life time of the winter wheat plant. Water stress prolonged the duration of stable growth, the more serious the water deficit, the longer the period of stable growth. The duration of stable growth was shortened and that of unstable growth was prolonged after water recovery. The growth behavior of the plants exposed to moderate water deficit shifted from stable to unstable until the end of the growth, after rewatering at flowering. In the life-time of the crop, the root and shoot had been adjusting themselves in structure and function so as to maintain an equilibrium, but could not achieve the equilibrium state for long. They were always in an unbalanced state from the beginning to the end of growth. This was the essence of root-shoot equilibrium. Water stress inhibited the function of root and shoot, reduced root shoot interactions, and as a result, the plant growth gradually tended to stabilize. Rewatering enhanced root shoot interactions, prolonged duration of instable growth. Rewatering at flowering could upset the inherent relativity during the long time of stable growth from flowering to filling stage, thus leading to unstable growth and enhanced dry matter accumulating rate in the whole plant.
基金supported by funds of the National Natural Science Foundation of China(U22A6009,32201824).
文摘Common wheat is a staple food for 35%of the global population,therefore increasing wheat yield in an ever-changing environment is essential for food security in the present day(Peng et al.,2011).Root system is responsible for water and nutrient acquisition,thus crucial for competitive fitness and crop yield in challenging environments(Karlova et al.,2021;Liu et al.,2022).Over the past decades,extensive research has focused on identifying genes accountable for root growth and development in plants(Rogers and Benfey,2015).However,only a limited number of genes have been cloned in wheat(Li et al.,2021).
基金funded by a project of the National Natural Science Foundation of China(31471443)the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)。
文摘To elucidate the response of oxidative metabolism,triggered by elevated ammonium(NH_(4)^(+))concentrations,on root growth of wheat seedlings,Yumai 49(NH_(4)^(+)-tolerant)and Lumai 15(NH_(4)^(+)-sensitive)cultivars were supplied with either 5.0 mmol L^(–1)NH_(4)^(+)-N(EAC)or 5.0 mmol L–1 NO_(3)–-N(CON)under hydroponic conditions.Root growth in both cultivars was significantly reduced under EAC,and the negative effect was greater in Lumai 15.EAC enhanced the activities of monodehydroascorbate reductase and dehydroascorbate reductase in the roots of both cultivars,while it decreased ascorbic acid(ASA)content and GDP-mannose pyrophosphorylase(GMPase)activity at the 12 th day after treatment in Lumai 15 by 62.0 and 71.4%;and in Yumai 49 by 38.8 and 62.2%,respectively,indicating that the regeneration of ASA was increased,but the biosynthesis of ASA was reduced under EAC treatment.Moreover,EAC increased DHA/ASA,reactive oxygen species(ROS),and malondialdehyde contents,as well as antioxidant enzyme activities in the roots of both cultivars.Relatively greater increases in ROS and soluble sugar,and lower antioxidant enzyme activities in Lumai 15 indicate severe disruption of oxidative metabolism when compared to Yumai 49.Results reveal that the reduction of ASA biosynthesis via decreased GMPase activity under the EAC condition probably acts as a trigger for accumulated ROS and imbalanced redox status,resulting in root growth inhibition during wheat seedling growth stage.Yumai 49,being an NH_(4)^(+)-tolerant cultivar,had the stronger capacity to protect itself from oxidative stress,which allowed it to retain a lower DHA to ASA ratio by maintaining a better redox homeostasis than could be maintained in the NH_(4)^(+)-sensitive cultivar Lumai 15.
基金supported by the Biotechnology and Biological Sciences Research Council,UK(BB/M001806/1,BB/L026848/1,BB/P026834/1,and BB/M019837/1)(MJB,DMW,and MPP)the Leverhulme Trust,UK(RPG-2016–409)(MJB and DMW)+1 种基金the European Research Council FUTUREROOTS Advanced Investigator Grant,UK(294729)to MG,JAA,DMW,and MJBthe University of Nottingham Future Food Beacon of Excellence,UK。
文摘The root system architecture(RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat(Triticum aestivum L.). Here, a high-throughput germination paper-based plant phenotyping system was used to identify seedling traits in a wheat doubled haploid mapping population, Savannah×Rialto. Significant genotypic and nitrate-N treatment variation was found across the population for seedling traits with distinct trait grouping for root size-related traits and root distribution-related traits. Quantitative trait locus(QTL) analysis identified a total of 59 seedling trait QTLs. Across two nitrate treatments, 27 root QTLs were specific to the nitrate treatment. Transcriptomic analyses for one of the QTLs on chromosome 2 D, which was found under low nitrate conditions, revealed gene enrichment in N-related biological processes and 28 differentially expressed genes with possible involvement in a root angle response. Together, these findings provide genetic insight into root system architecture and plant adaptive responses to nitrate, as well as targets that could help improve N capture in wheat.
