A total of 472 isozyme samples and about 1 000 myogen, esterase and morphyological samples of nine spawning stocks were collected from coastal waters of Hainan, Guangdong, Fujian, Zhejiang, Jiangsu and Sbandong Provin...A total of 472 isozyme samples and about 1 000 myogen, esterase and morphyological samples of nine spawning stocks were collected from coastal waters of Hainan, Guangdong, Fujian, Zhejiang, Jiangsu and Sbandong Provinces of China during 1976￣1992. Eleven isozymes were analysed by starch-gel and polyacrylamide-gel electrophoresis. Biochemical genetic structures of 11 isozymes were analysed and 24 loci were recorded. According to the allele frequencies of individual isozymes of each sampling stock, the mean proportion of polymorphic (P) and average heterozygosity (H) were calculated; then the genetic similarity (I) and genetic distance (D) were obtained. The results of cluster for O and D of all the samplied stocks show that the hairtail fish from Chemise coastal waters should be classified into three different sped6s (D: 0. 223￣0. 382) and eight populations (D: 0. 007￣0. 048).展开更多
For comprehensive understanding of practical application and evaluation on the power of30 commonly used InDeis(Qiagen Investigator DIPplex®kit),we captured population data from 25 Chinese populations and employed...For comprehensive understanding of practical application and evaluation on the power of30 commonly used InDeis(Qiagen Investigator DIPplex®kit),we captured population data from 25 Chinese populations and employed F-statistics for population genetics analysis.The results indicated that the distributions of allelic frequencies among populations were in different levels.Furthermore,the phylogeny confoiming pairwise FST distances showed that the difierentiation of majority populations were consistent with their geographic locations and historic dispersals.We conduct the comprehensive correlation analysis between FST and heterozygosity of30 InDel loci and provided strong evidence for ongoing InDei loci selection.The Fst values of 30 InDels were calculated within 25 Chinese populations,and then,these loci were characterized definitely based on their roles in population genetics or individual identification.Data indicated that 17 InDels with FST<0.01 could be utilized regarding Chinese individual identification(total discrimination power=0.999985 and cumulative matching probability=0.00000009).We comprehensively reconstructed the population structure and filled the gap of evaluating the ability of InDels in personal as well as population identification.The application of InDel loci in the forensic area would convincingly promote the development matter of forensic population identification and personal discrimination.展开更多
文摘A total of 472 isozyme samples and about 1 000 myogen, esterase and morphyological samples of nine spawning stocks were collected from coastal waters of Hainan, Guangdong, Fujian, Zhejiang, Jiangsu and Sbandong Provinces of China during 1976￣1992. Eleven isozymes were analysed by starch-gel and polyacrylamide-gel electrophoresis. Biochemical genetic structures of 11 isozymes were analysed and 24 loci were recorded. According to the allele frequencies of individual isozymes of each sampling stock, the mean proportion of polymorphic (P) and average heterozygosity (H) were calculated; then the genetic similarity (I) and genetic distance (D) were obtained. The results of cluster for O and D of all the samplied stocks show that the hairtail fish from Chemise coastal waters should be classified into three different sped6s (D: 0. 223￣0. 382) and eight populations (D: 0. 007￣0. 048).
基金This study was financially supported by The Fundamental Research Funds for the Central Research Institutes with project number“2017JB004.”
文摘For comprehensive understanding of practical application and evaluation on the power of30 commonly used InDeis(Qiagen Investigator DIPplex®kit),we captured population data from 25 Chinese populations and employed F-statistics for population genetics analysis.The results indicated that the distributions of allelic frequencies among populations were in different levels.Furthermore,the phylogeny confoiming pairwise FST distances showed that the difierentiation of majority populations were consistent with their geographic locations and historic dispersals.We conduct the comprehensive correlation analysis between FST and heterozygosity of30 InDel loci and provided strong evidence for ongoing InDei loci selection.The Fst values of 30 InDels were calculated within 25 Chinese populations,and then,these loci were characterized definitely based on their roles in population genetics or individual identification.Data indicated that 17 InDels with FST<0.01 could be utilized regarding Chinese individual identification(total discrimination power=0.999985 and cumulative matching probability=0.00000009).We comprehensively reconstructed the population structure and filled the gap of evaluating the ability of InDels in personal as well as population identification.The application of InDel loci in the forensic area would convincingly promote the development matter of forensic population identification and personal discrimination.
