Pathogenic Escherichia coli cause chicken colibacillosis, which is economically devastating to the poultry in- dustry worldwide （Bagheri et al., 2014）. Owing to in- creasing antibiotic resistance, phage therapy reag...Pathogenic Escherichia coli cause chicken colibacillosis, which is economically devastating to the poultry in- dustry worldwide （Bagheri et al., 2014）. Owing to in- creasing antibiotic resistance, phage therapy reagents have been developed to treat bacterial infections （Xu et al., 2015）.展开更多
Objective:To isolate virulent bacteriophage from environment samples and explore the potential way of decontaminating the environmental wastewater. Methods: The standard plaque assay, negative staining transmission el...Objective:To isolate virulent bacteriophage from environment samples and explore the potential way of decontaminating the environmental wastewater. Methods: The standard plaque assay, negative staining transmission electron microscopy (TEM), genome extraction and nucleases test were used to isolate bacteriophages. Then its morphology and characteristics were examined. Results: A novel bacteriophage XY-1 was isolated from a sewage pond in a hospital. It infected and killed 6 E.coli reference strains. The phage had a round head (diameter 40-50 nm), no tail and the genome was ssRNA of approximately 5.0 kb. It was able to reduce E.coli to an extent of 44.63% to 67.00% when being added into the samples of different raw sewage water, depending on the contact time, the temperature and the phage dose. Conclusion: From the morphology typical and nucleotide characteristics (RNA) of the genome of phage, phage XY-1 appears to be closely related to phage f2. It may have some effects for the control of E.coli in sewage water.展开更多
DNA水凝胶具有序列可编程性、分子识别能力、刺激响应性、生物相容性和生物降解性等独特性质,广泛应用于材料科学与生物医用领域。滚环扩增(rolling circle amplification,RCA)反应是一种简单高效的等温酶促扩增策略,可合成具有大量重...DNA水凝胶具有序列可编程性、分子识别能力、刺激响应性、生物相容性和生物降解性等独特性质,广泛应用于材料科学与生物医用领域。滚环扩增(rolling circle amplification,RCA)反应是一种简单高效的等温酶促扩增策略,可合成具有大量重复功能单元的超长DNA单链。基于RCA技术,研究人员构建了一系列智能DNA水凝胶材料,具有广阔的生物医学应用前景。本文综述了通过RCA反应制备具有多功能的智能DNA水凝胶的方法,含多聚适配体的DNA水凝胶和生物颗粒的特异性识别与分离,及其后续在疾病诊疗领域的应用研究。展开更多
基金supported by grants from the Nature Science Foundation of Shandong Province of China (grant nos.ZR2013CQ024 and ZR2015CM020)
文摘Pathogenic Escherichia coli cause chicken colibacillosis, which is economically devastating to the poultry in- dustry worldwide （Bagheri et al., 2014）. Owing to in- creasing antibiotic resistance, phage therapy reagents have been developed to treat bacterial infections （Xu et al., 2015）.
基金Supported by the National Natural Science Foundation ofChina (No. 30571637) the Scientific Research Project ofthe Tenth Five-year Plan of PLA (No. 01MA170)
文摘Objective:To isolate virulent bacteriophage from environment samples and explore the potential way of decontaminating the environmental wastewater. Methods: The standard plaque assay, negative staining transmission electron microscopy (TEM), genome extraction and nucleases test were used to isolate bacteriophages. Then its morphology and characteristics were examined. Results: A novel bacteriophage XY-1 was isolated from a sewage pond in a hospital. It infected and killed 6 E.coli reference strains. The phage had a round head (diameter 40-50 nm), no tail and the genome was ssRNA of approximately 5.0 kb. It was able to reduce E.coli to an extent of 44.63% to 67.00% when being added into the samples of different raw sewage water, depending on the contact time, the temperature and the phage dose. Conclusion: From the morphology typical and nucleotide characteristics (RNA) of the genome of phage, phage XY-1 appears to be closely related to phage f2. It may have some effects for the control of E.coli in sewage water.
文摘DNA水凝胶具有序列可编程性、分子识别能力、刺激响应性、生物相容性和生物降解性等独特性质,广泛应用于材料科学与生物医用领域。滚环扩增(rolling circle amplification,RCA)反应是一种简单高效的等温酶促扩增策略,可合成具有大量重复功能单元的超长DNA单链。基于RCA技术,研究人员构建了一系列智能DNA水凝胶材料,具有广阔的生物医学应用前景。本文综述了通过RCA反应制备具有多功能的智能DNA水凝胶的方法,含多聚适配体的DNA水凝胶和生物颗粒的特异性识别与分离,及其后续在疾病诊疗领域的应用研究。
